scholarly journals Antifungal activity of some Mediterranean seaweed against Macrophomina phaseolina and Fusarium oxysporum in Vitro

2020 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Asmaa Lotfi ◽  
Metwally Kottb ◽  
Ahmed Elsayed ◽  
Hesham Shafik
2017 ◽  
Vol 6 (9) ◽  
pp. 1676 ◽  
Author(s):  
Ramaraju Cherkupally ◽  
Srinivasa Reddy Kota ◽  
Hindumathi Amballa ◽  
Bhumi Narasimha Reddy

The antifungal activity of aqueous extracts of nine plants viz, Azadirachta indica, Parthenium hysterophorus, Momordica charantia, Allium sativum, Eucalyptus globules, Calotropis procera, Aloe vera, Beta vulgaris and Datura stramonium were assessed in vitro against Fusarium oxysporum f. sp. melongenae, Rhizoctonia solani and Macrophomina phaseolina, the soil borne phytopathogens. The assessment of fungitoxic effect was carried out by using three different concentrations i.e., 5, 10 and 20% against the test fungi, in terms of percentage of mycelial growth inhibition. The extract of A. sativum completely inhibited the mycelial growth of M. phaseolina at all the concentrations. The extracts of D. stramonium and E. globulus inhibited the mycelial growth of R. solani of 72%, and 70.7% respectively at 20% concentration, that of A. sativum, E. globulus and D. stramonium exhibited inhibition percentage of 63.3%, 61.8% and 61.1% respectively at 20% concentration on Fusarium oxysporum f. sp. melongenae. The application of plant extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.


2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Vivek Ahluwalia ◽  
Nandini Garg ◽  
Birendra Kumar ◽  
Suresh Walia ◽  
Om P. Sati

Vanillin oxime- N-O-alkanoates were synthesized following reaction of vanillin with hydroxylamine hydrochloride, followed by reaction of the resultant oxime with acyl chlorides. The structures of the compounds were confirmed by IR, 1H, 13C NMR and mass spectral data. The test compounds were evaluated for their in vitro antifungal activity against three phytopathogenic fungi Macrophomina phaseolina, Rhizoctonia solani and Sclerotium rolfsii by the poisoned food technique. The moderate antifungal activity of vanillin was slightly increased following its conversion to vanillin oxime, but significantly increased after conversion of the oxime to oxime- N-O-alkanoates. While vanillin oxime- N-O-dodecanoate with an EC50 value 73.1 μg/mL was most active against M. phaseolina, vanillin oxime- N-O-nonanoate with EC50 of value 66.7 μg/mL was most active against R. solani. The activity increased with increases in the acyl chain length and was maximal with an acyl chain length of nine carbons.


2015 ◽  
Vol 75 ◽  
pp. 150-158 ◽  
Author(s):  
D. Jasso de Rodríguez ◽  
F.A. Trejo-González ◽  
R. Rodríguez-García ◽  
M.L.V. Díaz-Jimenez ◽  
A. Sáenz-Galindo ◽  
...  

2010 ◽  
Vol 65 (7-8) ◽  
pp. 433-436
Author(s):  
Hui Xu ◽  
Huan Qu

Several 2,6-bis-(un)substituted phenoxymethylpyridines were synthesized and evaluated in vitro against Fusarium graminearum, Helminthosporium sorokinianum, Alternaria brassicae, Alternaria alternata, and Fusarium oxysporum f. sp. vasinfectum. Among all derivatives, compound 3 a exhibited a broad-spectrum antifungal activity against the five phytopathogenic fungi.


2020 ◽  
Vol 11 (5) ◽  
pp. 1135-1147
Author(s):  
Talina Olivia Martínez-Martínez ◽  
Brenda Zulema Guerrero-Aguilar ◽  
Víctor Pecina-Quintero ◽  
Enrique González-Pérez ◽  
Juan Gabriel Angeles-Núñez

El garbanzo es una leguminosa, que se cultiva en dos regiones de México principalmente, noroeste (Sonora, Sinaloa y Baja california) y la región de El Bajío (Guanajuato, Michoacán y Jalisco); sin embargo, cada año la producción del cultivo está comprometida con la fusariosis vascular, una de las principales enfermedades que afectan al cultivo y que está asociada al complejo fúngico Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Macrophomina phaseolina y Sclerotium rolfsii. Una alternativa de control biológico es la aplicación de Trichoderma, la que además tiene un efecto indirecto en la nutrición de la planta. El objetivo de este estudio fue determinar el antagonismo in vitro de dos cepas de Trichoderma harzianum (T1 y T2) y su efecto como biofertilizante. Se realizaron confrontaciones in vitro contra cepas de las razas de Fusariumoxysporum f. sp. ciceris (Foc 0, 1B/C, 5 y 6), Fusarium solani, Macrophomina phaseolina (MSonora y M-GTO) y Sclerotium rolfsii. Se evaluó el efecto de T2 como biofertilizante (TB) midiendo las variables: número de flores, vainas, altura de la planta, diámetro del tallo, longitud de la raíz y rendimiento de grano. Las dos cepas de T. harzianum mostraron antagonismo en diferente escala contra los patógenos. Adicionalmente, con el tratamiento donde se aplicó T. harzianum (TB) se presentaron incrementos en el número de flores (30%), vainas (24%), altura (3%), diámetro de las plantas (3.5%), así como la longitud de la raíz (13%) y rendimiento del grano (23%).


2012 ◽  
Vol 59 (2) ◽  
pp. 51-58 ◽  
Author(s):  
Alicja Saniewska ◽  
Anna Jarecka ◽  
Zbigniew Biały ◽  
Marian Jurzysta

Antifungal activity of total saponins originated from roots of <i>Medicago hybrida</i> (Pourret) Trautv. were evaluated <i>in vitro</i> against six pathogenic fungi and eight individual major saponin glycosides were tested against one of the most susceptible fungi. The total saponins showed fungitoxic effect at all investigated concentrations (0.01%, 0.05% and 0.1%) but their potency was different for individual fungi. The highest saponin concentration (0.1%) was the most effective and the inhibition of <i>Fusarium oxysporum</i> f. sp. <i>callistephi</i>, <i>Botrytis cinerea</i>, <i>Botrytis tulipae</i>, <i>Phoma narcissi</i>, <i>Fusarium oxysporum</i> f. sp. <i>narcissi</i> was 84.4%, 69.9%, 68.6%, 57.2%, 55.0%, respectively. While <i>Fusarium oxysporum</i> Schlecht., a pathogen of <i>Muscari armeniacum</i>, was inhibited by 9.5% only. Eight major saponin glycosides isolated from the total saponins of <i>M. hybrida</i> roots were tested against the mycelium growth of <i>Botrytis tulipae</i>. The mycelium growth of the pathogen was greatly inhibited by hederagenin 3-O-<i>β</i>-D-glucopyranoside and medicagenic acid 3-O-<i>β</i>-D-glucopyranoside. Medicagenic acid 3-O-<i>β</i>-D-glucuronopyranosyl-28-O-<i>β</i>-D-glucopyranoside and oleanolic acid 3-O-[<i>β</i>-D-glucuronopyranosyl(1→2)-<i>α</i>-L-galactopyranosyl]-28-O-<i>β</i>-D-glucopyranoside showed low fungitoxic activity. Medicagenic acid 3-O-a-D-glucopyranosyl- 28-O-β-D-glucopyranoside, hederagenin 3-O-[α-L- hamnopyranosyl(1→2)-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]- 28-O-α-D-glucopyranoside and hederagenin 3-O-<i>β</i>-D-glucuronopyranosyl-28-O-<i>β</i>-D- lucopyranoside did not limit or only slightly inhibited growth of the tested pathogen. While 2<i>β</i>, 3<i>β</i>-dihydroxyolean-12 ene-23-al-28-oic acid 3-O-<i>β</i>-D-glucuronopyranosyl-28-O-<i>β</i>-D-glucopyranoside slightly stimulated mycelium growth of <i>B. tulipae</i>.


2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Laila Muñoz Castellanos ◽  
Nubia Amaya Olivas ◽  
Juan Ayala-Soto ◽  
Carmen Miriam De La O Contreras ◽  
Miriam Zermeño Ortega ◽  
...  

In this study, hydrodistillation was used to obtain essential oils (EOs) from pepper (Piper nigrum L.) and clove (Eugenia caryophyllata) and co-hydrodistillation (addition of fatty acid ethyl esters as extraction co-solvents) was used to obtain functional extracts (FEs). Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger. The results showed that pepper (Piper nigrum) and clove (Eugenia caryophyllata) essential oils and their functional extracts are effective in vitro at concentrations from 400 to 500 ppm after 10 days of culturing. The essential oils and functional extracts were used on tomato fruit samples at three different concentrations: 350, 400, and 450 ppm5. Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%. The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C10), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi. Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β-caryophyllene in pepper oil.


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