Bacteriological Profile of Urinary Tract Infections and Antibiotic Resistance Profile in the Telagh Region (West Algeria)

Aims: This study investigated the antibiotic resistance profile of Escherichia coli from the urine of patients with suspected urinary tract infections in Federal Medical Centre, Keffi, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: Sample was obtained from the Federal Medical Center, Keffi and analyzed at Nasarawa State University, Keffi, Nigeria, between January and April 2018. Methodology: Three hundred and eighty urine samples were collected and E. coli was isolated and identified using standard microbiological methods. Antimicrobial Susceptibility Testing for the isolates was carried out and interpreted as described by the Clinical and Laboratory Standards Institute. Results: The occurrence of the bacterium was 12.9% (49/380). The occurrence in relation to the gender of the patients was higher in the female (15.5%) than the male (9.8%); in relation to age, it was highest at 11-20 years (23.5%) but lowest at > 50 years (2.3%). The isolates were more resistant to ampicillin (81.6%), streptomycin and sulphamethoxazole/ trimethoprime (75.0%) but less resistant to gentamycin (30.6%), and imipenem (22.4%). The occurrences of different classes of resistance were multidrug resistance (MDR) (93.9%) and pan drug resistance (4.2%). Most of the isolates were more resistant to the commonly prescribed antibiotic and were also MDR isolates. Conclusion: The need to review antibiotic use by the hospital is thus justified.

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Community- and Hospital-Acquired Klebsiella pneumoniae Urinary Tract Infections in Portugal: Virulence and Antibiotic Resistance

Microorganisms ◽

10.3390/microorganisms7050138 ◽

2019 ◽

Vol 7 (5) ◽

pp. 138 ◽

Cited By ~ 17

Author(s):

Cátia Caneiras ◽

Luis Lito ◽

José Melo-Cristino ◽

Aida Duarte

Keyword(s):

Antibiotic Resistance ◽

Urinary Tract ◽

Klebsiella Pneumoniae ◽

Urinary Tract Infections ◽

Multidrug Resistant ◽

Hospital Environment ◽

Virulence Determinants ◽

Antibiotic Resistance Profile ◽

Tract Infections ◽

Hospital Acquired

Klebsiella pneumoniae is a clinically relevant pathogen and a frequent cause of hospital-acquired (HA) and community-acquired (CA) urinary tract infections (UTI). The increased resistance of this pathogen is leading to limited therapeutic options. To investigate the epidemiology, virulence, and antibiotic resistance profile of K. pneumoniae in urinary tract infections, we conducted a multicenter retrospective study for a total of 81 isolates (50 CA-UTI and 31 HA-UTI) in Portugal. The detection and characterization of resistance and virulence determinants were performed by molecular methods (PCR, PCR-based replicon typing, and multilocus sequence typing (MLST)). Out of 50 CA-UTI isolates, six (12.0%) carried β-lactamase enzymes, namely blaTEM-156 (n = 2), blaTEM-24 (n = 1), blaSHV-11 (n = 1), blaSHV-33 (n = 1), and blaCTX-M-15 (n = 1). All HA-UTI were extended-spectrum β-lactamase (ESBL) producers and had a multidrug resistant profile as compared to the CA-UTI isolates, which were mainly resistant to ciprofloxacin, levofloxacin, tigecycline, and fosfomycin. In conclusion, in contrast to community-acquired isolates, there is an overlap between virulence and multidrug resistance for hospital-acquired UTI K. pneumoniae pathogens. The study is the first to report different virulence characteristics for hospital and community K. pneumoniae pathogens, despite the production of β-lactamase and even with the presence of CTX-M-15 ESBL, a successful international ST15 clone, which were identified in both settings. This highlights that a focus on genomic surveillance should remain a priority in the hospital environment.

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