In-vitro Antibiotic Resistance Profile of E. coli Strains Isolated from Community-acquired Paediatric Urinary Tract Infections in Karabük Province

Aims: This study investigated the antibiotic resistance profile of Escherichia coli from the urine of patients with suspected urinary tract infections in Federal Medical Centre, Keffi, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: Sample was obtained from the Federal Medical Center, Keffi and analyzed at Nasarawa State University, Keffi, Nigeria, between January and April 2018. Methodology: Three hundred and eighty urine samples were collected and E. coli was isolated and identified using standard microbiological methods. Antimicrobial Susceptibility Testing for the isolates was carried out and interpreted as described by the Clinical and Laboratory Standards Institute. Results: The occurrence of the bacterium was 12.9% (49/380). The occurrence in relation to the gender of the patients was higher in the female (15.5%) than the male (9.8%); in relation to age, it was highest at 11-20 years (23.5%) but lowest at > 50 years (2.3%). The isolates were more resistant to ampicillin (81.6%), streptomycin and sulphamethoxazole/ trimethoprime (75.0%) but less resistant to gentamycin (30.6%), and imipenem (22.4%). The occurrences of different classes of resistance were multidrug resistance (MDR) (93.9%) and pan drug resistance (4.2%). Most of the isolates were more resistant to the commonly prescribed antibiotic and were also MDR isolates. Conclusion: The need to review antibiotic use by the hospital is thus justified.

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Bacteriological Profile of Urinary Tract Infections and Antibiotic Resistance Profile in the Telagh Region (West Algeria)

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology ◽

10.21608/eajbsc.2021.211890 ◽

2021 ◽

Vol 13 (2) ◽

pp. 275-296

Author(s):

Zouaouia Chama ◽

Amina Benabbou ◽

Hadria Derras ◽

Nawel Benchiha ◽

Bouzid khadra ◽

...

Keyword(s):

Antibiotic Resistance ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Resistance Profile ◽

Antibiotic Resistance Profile ◽

Bacteriological Profile ◽

Tract Infections ◽

West Algeria

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In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

Applied Sciences ◽

10.3390/app11094315 ◽

2021 ◽

Vol 11 (9) ◽

pp. 4315

Author(s):

Emanuel Vamanu ◽

Laura Dorina Dinu ◽

Cristina Mihaela Luntraru ◽

Alexandru Suciu

Keyword(s):

Urinary Tract ◽

Bioactive Compounds ◽

Urinary Tract Infections ◽

Antimicrobial Effect ◽

Biologically Active ◽

Bacterial Strains ◽

E Coli ◽

Functional Product ◽

Tract Infections

Bioactive compounds and phenolic compounds are viable alternatives to antibiotics in recurrent urinary tract infections. This study aimed to use a natural functional product, based on the bioactive compounds’ composition, to inhibit the uropathogenic strains of Escherichia coli. E. coli ATCC 25922 was used to characterize the IVCM (new in vitro catheterization model). As support for reducing bacterial proliferation, the cytotoxicity against a strain of Candida albicans was also determined (over 75% at 1 mg/mL). The results were correlated with the analysis of the distribution of biologically active compounds (trans-ferulic acid-268.44 ± 0.001 mg/100 g extract and an equal quantity of Trans-p-coumaric acid and rosmarinic acid). A pronounced inhibitory effect against the uropathogenic strain E. coli 317 (4 log copy no./mL after 72 h) was determined. The results showed a targeted response to the product for tested bacterial strains. The importance of research resulted from the easy and fast characterization of the functional product with antimicrobial effect against uropathogenic strains of E. coli. This study demonstrated that the proposed in vitro model was a valuable tool for assessing urinary tract infections with E. coli.

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Determination of nitrofurantoin and fosfomycin susceptibility among urinary Escherichia coli isolates

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20203676 ◽

2020 ◽

Vol 8 (9) ◽

pp. 3274

Author(s):

Rachana Kanaujia ◽

Amit Kumar ◽

Malay Bajpai

Keyword(s):

Escherichia Coli ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Urine Samples ◽

E Coli ◽

Tract Infections ◽

Therapeutic Alternatives ◽

Micro Organisms ◽

Staphylococcus Spp

Background: Urinary tract infections (UTIs) are one of the most common infections. For treatment of UTIs, there are limited antibiotics due to increased resistance among uropathogens. Two older antibiotics; Nitrofurantoin and Fosfomycin have become novel oral therapeutic options against uropathogens. Aim of the study was to identify UTI causing micro-organisms and evaluate in-vitro activity of nitrofurantoin and fosfomycin against most common isolated organism (E. coli).Methods: Results of urine samples culture and susceptibility testing over a period of 1 year were analysed and included in this study.Results: Micro-organisms were isolated from 568 urine samples. Most commonly isolated organism was Escherichia coli (40.50%), followed by Klebsiella spp. (20.07%) and Staphylococcus spp. (17.07%). Susceptibility of E. coli to nitrofurantoin and fosfomycin was 91.74% and 65.65% respectively. Conclusion: Good activity of nitrofurantoin and fosfomycin against E. coli indicates that these two drugs are potential therapeutic alternatives for urinary tract infections.

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