Insecticidal Activity of seed extracts of Annona squamosa L., against the cotton leafworm Spodoptera littoralis (Boisd.)

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Mona Abdelzaher ◽  
Abdel-naser Hassan
Keyword(s):  
Insecticidal Activity ◽  
Spodoptera Littoralis ◽  
Annona Squamosa ◽  
Cotton Leafworm ◽  
Seed Extracts

scholarly journals Insecticidal Activity of Annona squamosa l. Seed Extracts against the Red Flour Beetle, Tribolium castaneum (Herbst)

1970 ◽  
Vol 14 ◽  
pp. 107-112 ◽  
Author(s):  
M Khalequzzaman ◽  
Shajia Sultana
Keyword(s):  
Tribolium Castaneum ◽  
Insecticidal Activity ◽  
Methanol Extract ◽  
Red Flour Beetle ◽  
Regression Equations ◽  
Annona Squamosa ◽  
Flour Beetle ◽  
Custard Apple ◽  
Larval Bioassay ◽  
Seed Extracts

Insecticidal activity of the seed extracts of custard apple, Annona squamosa L. in petroleum spirit, ethyl acetate, acetone and methanol against Raj, CR 1, FSS II and CTC-12 strains of the red flour beetle, Tribolium castaneum (Herbst) was studied. The seeds were dried, powdered and extracted in Soxhlet`s apparatus in the solvents below 60°C. Extractions were applied on larvae and adult beetles in film residue methods and mortality was recorded after 24 h. For larval bioassay the highest toxicity was recorded for petroleum spirit extract (LD50= 0.03?g cm-2) in Raj strain and the lowest toxicity was for methanol extract (LD50=15.697?g cm-2) in FSS II strain. In adults petroleum spirit extract offered highest toxicity (LD50= 58.697?g cm-2) in CTC 12 strain and the lowest toxicity (LD50=22004.710?g cm-2) was for acetone extract in CR 1 strain. LD50, 95% confidence limits and regression equations are presented. Key words: Tribolium castaneum, Annona squamosa, custard apple, LD50 J. bio-sci. 14: 107-112, 2006

Plant-lectins as insecticidal agents against cotton leaf worm Spodoptera littoralis and their potential applications in crop protection

2020 ◽  
Vol 10 ◽  
Author(s):  
Soad F. E. Mohsen ◽  
Moustafa A. Abbassy ◽  
Hamdy K Abou-Taleb ◽  
Entsar I. Rabea
Keyword(s):  
Insecticidal Activity ◽  
Spodoptera Littoralis ◽  
Crop Protection ◽  
Defense Responses ◽  
Kidney Bean ◽  
Cotton Leaf ◽  
Larval Duration ◽  
Cotton Leafworm ◽  
Wide Range

Background: The production of insecticidal proteins is one of the greatest vital direct defense responses in plants against the attack by phytophagous insects. One particular class of entomotoxic proteins present in many plant species is lectins that used against a wide range of important insects. Aim: The insecticidal activity of two lectins from seeds of white kidney bean (Phaseolus vulgaris) and soybean (Glycine max) on the growth, development and survival of the cotton leafworm, Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) was studied. Methods: Lectin products were isolated and purified using ammonium sulfate precipitation. Insecticidal activity, growthinhibitory and physiological effects on the differential haemocyte count of lectin products were evaluated against S. littoralis. In vivo effects of lectin products on some S. littoralis enzymes were also determined. Results: Lectin contents in 250 g seeds was 700 and 500 mg in soybean and white kidney bean, respectively. The total protein concentrations were 160-163 and 118-120 mg/mL in soybean and white kidney bean, respectively. The insecticidal activity of lectin products exerted a strong effect on the larval weight gain and the reduction of the weight reached to 75.25%. Furthermore, the other developmental stages of pupation and adult formation were also affected. Elongated larval duration was noticed since larval duration was 24.8 days compared with control (12.0 days). A complete pupation (100%) was recorded in the control however, a significant decrease in pupation was found to be 54.0% in treated larvae. Lectin products increased the average number of 123.9 haemocytes (123.9) compared to the control (94.4 haemocytes/field). In addition, lectin products inhibited the activity of total proteases, α-amylase, GST and total esterases. Conclusion: It can be concluded that the lectins with high potential for the use in pest control strategies based on their activity towards the cotton leafworm, S. littoralis. These findings suggest that lectin products are interesting tools for integrated pest management in important agriculture crops.

scholarly journals Insecticidal activity of chitosans of different molecular weights and chitosan-metal complexes against cotton leafworm Spodoptera littoralis and oleander aphid Aphis nerii

2012 ◽  
Vol 48 (No. 3) ◽  
pp. 131-141 ◽  
Author(s):  
M.E.I. Badawy ◽  
A.F. El-Aswad
Keyword(s):  
Metal Complexes ◽  
Insecticidal Activity ◽  
Spodoptera Littoralis ◽  
Larval Mortality ◽  
Systemic Effect ◽  
Agricultural Pests ◽  
Aphis Nerii ◽  
Cotton Leafworm ◽  
Molecular Weights ◽  
Chemically Modified

As an alternative to synthetic pesticides, chitosan has received much attention as a biopolymer active against some agricultural pests. The, insecticidal activity of chitosans of four molecular weights (2.27&nbsp;&times;&nbsp;10<sup>5</sup>,3.60 &times; 10<sup>5</sup>,5.97 &times; 10<sup>5</sup>,and9.47 &times; 10<sup>5 </sup>g/mol) was investigated against two species of arthropod pests: oleander aphid Aphis nerii and cotton leafworm Spodoptera littoralis. In addition, the most active chitosan of 2.27 &times; 10<sup>5 </sup>g/mol was chemically modified with metals of Ag(I), Cu(II), Ni(II), and Hg(II) to give corresponding chitosan-metal complexes. Larval mortality, growth inhibition, and antifeedant activities for third instar larvae of S. littoralis were evaluated at 4 g (a.i.) chitosan/kg diet. Chitosan of 2.27 &times; 10<sup>5 </sup>g/mol and its complexes with Ni and Hg were the most active compounds. The results against A. nerii indicated that chitosans of 3.60&nbsp;&times; 10<sup>5</sup> and 5.97 &times; 10<sup>5&nbsp;</sup>g/mol showed high activity among the different molecular weights in leaf-dip bioassay after 24&nbsp;h of treatment with 48 and 49% mortalities, respectively, at 1000 mg/l. All compounds had a systemic effect against A.&nbsp;nerii. Chitosans of 2.27 &times; 10<sup>5</sup>,3.60 &times; 10<sup>5</sup>,and5.97 &times; 10<sup>5 </sup>g/mol showed the highest efficacy at all concentrations tested; however, chitosan-Cu was significantly the most active among the complexes.

scholarly journals Sublethal effects of emamectin benzoate on life table parameters of the cotton leafworm, Spodoptera littoralis (Boisd.)

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
El-Sayed Mokbel ◽  
Amal Huesien
Keyword(s):  
Life Table ◽  
Spodoptera Littoralis ◽  
Sublethal Effects ◽  
Lethal Effect ◽  
Reproductive Rate ◽  
Relative Fitness ◽  
Emamectin Benzoate ◽  
Cotton Leafworm ◽  
Sublethal Concentrations ◽  
Preovipositional Period

Abstract Background The cotton leafworm, Spodoptera littoralis (Boisd.), is a serious economic pest in Egypt. Pest control depends mainly on chemical control with several pesticides include conventional and modern insecticides. Comprehensive analysis of pesticides impacts needs to investigate sublethal effects in addition to lethal effect. Results In the current study, the leaf-dip bioassay method was used to evaluate emamectin benzoate (EMB) sublethal concentrations. Results showed that EMB proved high toxicity against S. littorals with LC50 value of 0.019 mg liter−1. Life table analysis showed that treatments with LC5 and LC15 prolonged larval period, mean longevity of males and females, mean generation time (T), doubling time (DT), adult preovipositional period (APOP), and total preovipositional period (TPOP) compared with control. On the contrary, net reproduction rates (R0), intrinsic rates of increase (r), finite rate (λ), fecundity, gross reproductive rate (GRR), and relative fitness were decreased compared to control. Conclusions The current study clarified that sublethal concentrations of EMB induce adverse effects and suppress the population growth of S. littorals. Our results would be useful to assess the overall effects of EMB on S. littorals and can contribute effectively in pest management.

scholarly journals Efficacy of the parasitoid, Trichogrammatoidea bactrae Nagaraja (Hymenoptera: Trichogrammatidae) on the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) egg masses

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Hend O. Mohamed
Keyword(s):  
Spodoptera Littoralis ◽  
Insect Pests ◽  
Single Layer ◽  
Control Agent ◽  
Physical Characteristics ◽  
Great Ability ◽  
Egg Masses ◽  
Cotton Leafworm ◽  
Lepidoptera Noctuidae ◽  
Trichogrammatoidea Bactrae

Abstract Background The Egyptian cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the major insect pests, causing a significant damage on different cultivated agricultural crops. Developing an alternative non-chemical tool, an effective and environmentally friendly method to suppress pest's infestation is essentially needed. Therefore, biological control by releasing the egg parasitoids could be the most promising tool for integrated pest management. Results This study was designed to evaluate the efficacy of the egg parasitoid, Trichogrammatoidea bactrae Nagaraja (Hymenoptera: Trichogrammatidae) as a bio-control agent against S. littoralis egg masses with different physical characteristics (number of egg layer and degree of scale density) in a no-choice and choice tests, under laboratory conditions. Also, the parasitoids’ fitness in terms of parasitism percentage, developmental period, adults’ emergence percentage, female offspring percentage, and longevity were investigated. The results revealed that T. bactrae wasps had a great ability to parasitize S. littoralis egg masses, but with different rates, related to their layers and scales’ thickness in both tests. The highest parasitism percentage was observed on one-layer eggs, followed by two layers. However, 3-layer eggs were the least preferable one. High numbers of adult emergencies (> 80%) were observed in all tested egg masses, except in the case of 3 layers with high scales. Furthermore, female-biased sex ratios were noticed at all examined eggs, with only the exception of high-scaly eggs with a single layer that recorded the lowest rate (≤ 45%). Besides, the survival of adult female parasitoids was not significantly affected in both tests. Conclusions T. bactrae could be used as a bio-control agent against S. littoralis egg masses with different physical characteristics based on the achieved results.

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