scholarly journals The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

2017 ◽  
Vol 40 (4) ◽  
pp. 405-426 ◽  
Author(s):  
Nesrine Ebrahim ◽  
Ola Mohammed ◽  
Arigue Dessouky ◽  
Dina Abdel Fatah
Keyword(s):  
Stem Cells ◽  
Therapeutic Effect ◽  
Amniotic Membrane ◽  
Platelet Rich Plasma ◽  
Experimentally Induced

Therapeutic effect of mesenchymal stem cells on experimentally induced hypertensive cardiomyopathy in adult albino rats

2016 ◽  
Vol 41 (1) ◽  
pp. 36-50 ◽  
Author(s):  
Nariman A. Khater ◽  
Sally A. Selim ◽  
Samia A. Abd El-Baset ◽  
Samar H. Abd El Hameed
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Effect ◽  
Albino Rats ◽  
Experimentally Induced

scholarly journals Platelet-rich plasma enhanced MSCs repair for glycerin-induced acute kidney injury via AKT/Rab27 paracrine pathway

2020 ◽  
Author(s):  
Cheng Ji ◽  
Jiahui Zhang ◽  
Zixuan Zhou ◽  
Hui Shi ◽  
wanzhu Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Kidney Injury ◽  
Mesenchymal Stem Cells ◽  
Renal Function ◽  
Therapeutic Effect ◽  
Platelet Rich Plasma ◽  
Kidney Injury ◽  
Human Umbilical Cord ◽  
Renal Tubular Cells

Abstract Background: Acute kidney injury (AKI) was defined by rapid deterioration of renal function, as a common complication in hospitalized patients. Among the recent therapeutic options, mesenchymal stem cells (MSCs) were considered a promising therapeutic strategy for damaged tissues repair. Platelet rich plasma (PRP) regulates stromal cells to repair tissue damage through the release of growth factors. Here we proposed a possible therapeutic use of human umbilical cord mesenchymal stem cells stimulated by platelet-rich plasma (PRP-MSCs) in glycerin-induced acute kidney injury murine model.Methods: In vivo, we constructed glycerin-induced acute kidney injury rat models. On day 1 post injury, rat received a tail vein injection of 1×106 MSCs and 1×106 PRP-MSCs. All animals were sacrificed at Day 3 after glycerin injection. In vitro, NRK-52E cells were damaged by 20% glycerol for 12 hours, after that NRK-52E incubated with MSCs and PRP-MSCs for 24 hours in transwell co-culture system, DMEM as a negative control. NRK-52E cells were harvested for apoptosis assay, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR). Then the number of MSCs exosomes stimulated by PRP was detected by confocal microscopy and Nanosight tracking analysis (NTA), PRP-MSCs-Ex (10mg/kg) and MSCs-Ex (10mg/kg) were injected intravenously, saline as control. The therapeutic effect of PRP-MSCs was evaluated by analyzing renal function (serum BUN, Creatinine), histopathological structure changes and apoptosis and proliferation of renal tubular cells. Results: In vivo and vitro studies confirmed that the PRP induced YAP nucleus expression to promoting the proliferation and reinforces the stemness of MSCs, and PRP could promoted the paracrine secretion of exosomes by MSCs to repair AKI though AKT/Rab27 pathway.Conclusions: Our results revealed that PRP stimulated MSCs paracrine pathway could effectively alleviate glycerin-induced acute kidney injury. Therefore, RPP pretreatment may be a new method to improve the therapeutic effect of MSCs.

scholarly journals Comparative Histological Study on the Therapeutic Effect of Green Tea and Stem Cells in Alzheimer’s Disease Complicating Experimentally Induced Diabetes

2015 ◽  
Vol 8 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Hend Shafik Bassiony ◽  
Maha Baligh Zickri ◽  
Hala Gabr Metwally ◽  
Hala Ahmed Elsherif ◽  
Sarah Mohammed Alghandour ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Stem Cells ◽  
Therapeutic Effect ◽  
Green Tea ◽  
Histological Study ◽  
Experimentally Induced
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