Abstract
BackgroundThe high content of oil and protein makes peanut the main oil and edible crop in the world. Root-knot nematode forms root-knot by infecting peanut roots, which lead to poor development of peanut roots and seriously restricts the yield of peanut in the world. With the release of peanut genome, a large number of genetic loci controlling peanut root-knot nematode have been detected, but the molecular mechanism of root-knot nematode is still unclear. ResultsThe whole transcriptome RNA-seq was used to reveal the divergent response to root-knot nematode stress in peanut roots. A total of 430 mRNAs, 111 miRNAs, 4453 lncRNAs and 123 circRNAs were identified differential expression between infected and no-infected peanut, respectively. To understand the potential mechanisms in response to root-knot nematodes in peanut roots, the expression profiles of lncRNA/circRNA-miRNA-mRNA network were constructed. A total of 10 lncRNAs, 4 circRNAs, 5 miRNAs and 13 mRNAs can regularly the expression of mRNA during root-knot nematodes stress by forming competing endogenous RNA and participate in oxidation-reduction process and other various biological metabolism pathways in peanut. The results gained will reveal the role of ceRNAs of peanut in response to root-knot nematodes.ConclusionThe GO classification and KEGG pathway enrichment analysis of core regulatory networks revealing the ceRNAs participate in oxidation-reduction, peroxidase activity, lignin synthesis in xylem and flavonoid synthesis process. Overall, those results could gain the knowledge of the role of no-coding RNAs in response to root-knot nematodes.
ROLE OF SPENT MUSHROOM AND COMPOST TO CONTROL ROOT-KNOT NEMATODES ON EGGPLANT
