Application methods of Steinernema feltiae, Xenorhabdus bovienii and Purpureocillium lilacinum to control root-knot nematodes in greenhouse tomato systems

2018 ◽  
Vol 108 ◽  
pp. 31-38 ◽  
Author(s):  
Ilker Kepenekci ◽  
Selcuk Hazir ◽  
Ercin Oksal ◽  
Edwin E. Lewis
Keyword(s):  
Steinernema Feltiae ◽  
Root Knot Nematodes ◽  
Greenhouse Tomato ◽  
Purpureocillium Lilacinum ◽  
Control Root ◽  
Application Methods

scholarly journals Comparison of a Vintage and a Recently Released Nematicide for the Control of Root-Knot Nematodes and Side Effects on Two Entomopathogenic Nematodes

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1491
Author(s):  
Ioannis O. Giannakou ◽  
Stefanos Kamaras
Keyword(s):  
Chemical Control ◽  
Entomopathogenic Nematodes ◽  
No Reduction ◽  
The Other ◽  
Steinernema Feltiae ◽  
Vegetable Crops ◽  
Egg Hatching ◽  
Root Knot Nematodes ◽  
Tomato Plants ◽  
First Time

Root-knot nematodes can cause tremendous losses in vegetable crops. Farmers usually rely on synthetic nematicides to protect their crops. Recently, newly released nematicides are giving farmers an alternative in chemical control for nematodes. In the present study, the efficacy of vintage nematicide was compared to that of a relatively new nematicide, fluopyram. The latter was always more effective in substantially lower concentrations than oxamyl. Fluopyram paralyzed more than 80% J2s after 24 h immersion at the concentration of 0.25 μL L−1, while the percentage was increased close to 100% after immersion for 48 and 96 h. Similar levels of dead J2s were observed after immersion of J2s in oxamyl at concentrations higher than 8 μL L−1 (24 and 48 h) or 4 μL L−1 (96 h). An evident decrease of egg differentiation was observed when fluopyram concentration was increased to 8 μL L−1, while no significant decrease in egg differentiation was recorded at any concentration of oxamyl. Egg hatching was decreased at concentrations of fluopyram higher than 4 μL L−1, while no reduction was observed even when the concentration of oxamyl was increased to 64 μL L−1. The efficacy of fluopyram in soil was superior compared to that of oxamyl. For the first time, the systemic action of fluopyram is recorded in trials with tomato plants. On the other hand, compared to oxamyl, fluopyram seems to be more toxic to non-target organisms such as the entomopathogenic nematodes Steinernema feltiae and Heterorhabditis bacteriophora.

scholarly journals Prevalence and Potential Impact of Soil-dwelling Pests and Insect Pathogenic Nematodes in Strawberry Fields

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1366-1370 ◽  
Author(s):  
James A. LaMondia ◽  
Richard S. Cowles ◽  
Lorraine Los
Keyword(s):  
Steinernema Feltiae ◽  
Pratylenchus Penetrans ◽  
Naturally Occurring ◽  
Percent Loss ◽  
Otiorhynchus Sulcatus ◽  
Lesion Nematodes ◽  
Azinphos Methyl ◽  
Control Root ◽  
Lesion Nematode ◽  
Potential Impact

Surveys mailed to strawberry growers in 1999 determined the state of nematode and root weevil awareness and practices for their management. Based on the survey response, 41 fields representative of various practices were selected for sampling throughout Connecticut. Adult black vine weevils (Otiorhynchus sulcatus) were found in only 3 fields, but notched leaves characteristic of their feeding were found in 40 fields, indicating a greater prevalence than perceived by growers. The percentage of notched leaves was positively correlated with years in production, suggesting that it took some time for the flightless weevils to migrate into and to increase to damaging numbers in fields. In fields older than 2 years, bifenthrin insecticide reduced leaf feeding compared to untreated fields or to fields treated with endosulfan or azinphos-methyl. Lesion nematodes (Pratylenchus penetrans) were detected in 31 fields and were present in about 58% of plants. When present, nematode numbers were greater in the margins of poor areas than in adjacent healthy plants (735 vs. 428 per g root, respectively). Lesion nematode numbers were also greater in replanted strawberries than rotated fields (760 vs. 304, respectively). Soil fumigation with methyl bromide, but not methyl dithiocarbamate or the combination of 1,3-dichloropropene and chloropicrin, reduced nematode densities in the following strawberry crop. Based on an economic model, nematodes reduced accumulated profit over 4 fruiting years by more than the percent loss of fruit yield. Beneficial insect pathogenic nematodes, predominantly Heterorhabditis bacteriophora and Steinernema feltiae, were found in 75% of fields to which commercially obtained nematodes had been applied, and to 14% of the remaining fields. Presence of naturally occurring insect pathogenic nematodes in strawberry fields may control root weevil populations and lead to more years of productivity.

scholarly journals Linking Organic Metabolites as Produced by Purpureocillium Lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes

2020 ◽  
Vol 12 (19) ◽  
pp. 8276
Author(s):  
Abhishek Sharma ◽  
Aditi Gupta ◽  
Manu Dalela ◽  
Satyawati Sharma ◽  
R. Z. Sayyed ◽  
...  
Keyword(s):  
Sustainable Management ◽  
Ethyl Acetate Extract ◽  
In Vivo Studies ◽  
Egg Mass ◽  
Root Knot Nematodes ◽  
Second Stage ◽  
Purpureocillium Lilacinum ◽  
Wide Range ◽  
Phenyl Ethyl Alcohol

Root-knot nematodes pose a serious threat to agriculture and forest systems, causing significant losses of the crop worldwide. A wide range of chemical nematicides has traditionally been used to manage phyto-nematodes. However, due to their ill effects on the environment, biological control agents (BCAs) like Purpureocillium lilacinum that exhibit antagonistic effects on root-knot nematodes are preferred. The current study focused on identifying nematicidal metabolites produced by the fungus Purpureocillium lilacinum cultivated on akaranja deoiled cake-based liquid medium through bioactivity-guided fractionation against Meloidogyne incognita. Column chromatography of the ethyl acetate extract of fungal filtrate exhibited the most potent fraction (fraction 14–15), giving 94.6% egg mass hatching inhibition on the 5th day and a maximum nematicidal activity of 62% against second-stage juveniles after 48 h at 5000 mg/L. Gas chromatography coupled with mass spectrometry (GC-MS) analysis of this fraction revealed five major compounds, viz., 2-ethyl butyric acid, phenyl ethyl alcohol, benzoic acid, benzene acetic acid, and 3,5-Di-t-butylphenol. These biocompounds have potential biocontrol applications in agriculture, but further in vivo studies are warranted.

Sign in / Sign up

Export Citation Format

Share Document