scholarly journals EFFECTS OF DIFFERENT CROTON (Codiaeum variegatum L.) GENOTYPES AND GROWTH REGULATORS ON CALLUS INDUCTION, MICRO PROPAGATION AND ANTIBACTERIAL ACTIVITIES

2018 ◽  
Vol 45 (1) ◽  
pp. 331-347
Author(s):  
Gehad Bakheet ◽  
S. Soliman ◽  
M. Abdelkader ◽  
M. Elashtokhy
2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.


Author(s):  
Nisha Malik ◽  
Rakesh Singh Sengar ◽  
Manoj Kumar Yadav ◽  
Shiv Kumar Singh ◽  
Gopal Singh ◽  
...  

2020 ◽  
Vol 2 (1) ◽  
pp. 48-56
Author(s):  
Salem Hammud ◽  
Moohamed Abosneena ◽  
Munder Abugnia ◽  
Ahmed Shaaban ◽  
Adel Elmograbi

This experiment was conducted in Biotechnology research center BTRC for the purpose of studying the possibility of propagate Adiantum Capillus plant by using spores through plant tissues culture technology, which is one of the endangered plants in Libya. MS media was used in this study supplemented with some growth regulators (cytokines) of benzyl adenine BA and Kinten K at different concentrations (0.0, 0.5, 1.0, 2.0) mg / l. The results indicate that the growth of spores represented by the increase in the length and size of the vegetative masses in the MS nutritional medium equipped with BA growth regulator at a concentration of 2.0 mg / l is significantly higher than other treatments. The obtained plants were adapted by using growth medium of the Betmos and sand mixture in a ratio of 1: 2 in the growth room. The branches and roots formed when the plant moved to the greenhouse in the same medium of development


2001 ◽  
Vol 1 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Hamid Rashid ◽  
Rizwana Abdul Ghani . ◽  
Zubeda Chaudhry .

2004 ◽  
Vol 7 (3) ◽  
pp. 331-334 ◽  
Author(s):  
Md. Monirul Islam ◽  
Sanjay Kumar Adhikar . ◽  
Purnendu Gain . ◽  
Md. Mizanur Rahman . ◽  
Noor-e-Alam Siddique .

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   


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