Micro propagation of Adiantum Capillus plant through a culture of plant spores

2020 ◽  
Vol 2 (1) ◽  
pp. 48-56
Author(s):  
Salem Hammud ◽  
Moohamed Abosneena ◽  
Munder Abugnia ◽  
Ahmed Shaaban ◽  
Adel Elmograbi
Keyword(s):  
Growth Regulators ◽  
Plant Tissues ◽  
Endangered Plants ◽  
Benzyl Adenine ◽  
Sand Mixture ◽  
Biotechnology Research ◽  
Nutritional Medium ◽  
Growth Room ◽  
Micro Propagation ◽  
Culture Technology

This experiment was conducted in Biotechnology research center BTRC for the purpose of studying the possibility of propagate Adiantum Capillus plant by using spores through plant tissues culture technology, which is one of the endangered plants in Libya. MS media was used in this study supplemented with some growth regulators (cytokines) of benzyl adenine BA and Kinten K at different concentrations (0.0, 0.5, 1.0, 2.0) mg / l. The results indicate that the growth of spores represented by the increase in the length and size of the vegetative masses in the MS nutritional medium equipped with BA growth regulator at a concentration of 2.0 mg / l is significantly higher than other treatments. The obtained plants were adapted by using growth medium of the Betmos and sand mixture in a ratio of 1: 2 in the growth room. The branches and roots formed when the plant moved to the greenhouse in the same medium of development

Micropropagation of Capparis spinosa through plant tissue culture technology

2020 ◽  
Vol 2 (1) ◽  
pp. 56-64
Author(s):  
Elmundr Abughnia ◽  
Salem Hammud ◽  
Ahmed Shaaban ◽  
Mohamed Abosneena ◽  
Adel Elmograbi
Keyword(s):  
Tissue Culture ◽  
Average Length ◽  
The Other ◽  
Peat Moss ◽  
Benzyl Adenine ◽  
Nutritional Medium ◽  
Capparis Spinosa ◽  
Culture Technology ◽  
Number Of Branches ◽  
Plant Length

The study was conducted to investigate the effect of adding different concentrations of plant growth regulators of Benzyl adenine BA, KIN and 2 iP to MS nutritional medium on the growth of single nodes of Capparis spinosa plant. The results showed that in plant length factor the treatments of 2 and 1 mg / liter 2iP were significantly higher compared with the other treatments and the obtained plants reached average length of 3,3.3,1 cm respectively in these treatments, while for the average number of branches and leaves factors, the treatment 2 mg / liter of BA was significantly higher compared with other treatments, the produced plants arrive to 3.6 branches and 14.1 leaves. For rooting system, the treatment of MS supplemented with IBA growth regulator with a concentration of 2 mg / liter was significantly higher for both length and number of roots. The obtained plants were adapted using different treatments but the treatment of mixture peat moss and sand 1: 2 was the best

scholarly journals Micropropagation of Zingiber officinal roscoe

2019 ◽  
pp. 38-50
Author(s):  
Kheiry Keer ◽  
Elmundr Abughnia ◽  
Salem Hammud ◽  
Ahmed shaaban ◽  
Mohamed Abusanina ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Control Treatment ◽  
Peat Moss ◽  
Local Market ◽  
Biotechnology Research ◽  
Micro Propagation ◽  
Culture Technology

This study was conducted in plant tissue culture In Biotechnology which belong to biotechnology research center (Tripoli – for Micropropagation of in order to study the response of Zingiber plant to In vitro micro propagation through plant tissue culture technology , while the study was beginning by Samples were collected , the samples from the local market and directly were put in dark For sprouting in order to obtain plant tissue which will be used for plant micro propagation. Sprouted buds growth were obtained the plant tissue were sterilized by use 2.5% Clorox and 70% ethanol in hood cabinet with sterilized conditions , then sterilized plant tissue were cultured in small gars contain Murashige and Skoog MS medim as control treatment and MS media supplemented with different concentrations of BA and NAA plant growth regulators while the treatments were ( 2 , 4mg/l BA ) and ( 2 mg/l BA + 0.5mg/l NAA) . Results of this study showed present a good response of Zingiber plant to micro propagation by tissue culture technology in all the treatments event control treatment moreover the results showed that the treatment of 2 mg/l BA gave the highest average of obtained number of brunches and root system growth , finally the obtained plants from the experiments were moved to adaptation stage by placed the plants in small puts contain peat moss fertilizer

β-Oxidation of certain growth regulators by plant tissues

1970 ◽  
Vol 1 (4) ◽  
pp. 132-137 ◽  
Author(s):  
J. L. Garraway
Keyword(s):  
Growth Regulators ◽  
Plant Tissues

Total chlorophyll, growth and productivity of soybean [Glycine Max (L) Merril] under different levels and sources of phosphorus and plant growth regulators in south Rajasthan, India

2016 ◽  
Author(s):  
Shankar Lal Khaswan ◽  
R. K. Dubey ◽  
R. C. Tiwari ◽  
S. K. Dubey ◽  
Raj Kumari Chaudhary
Keyword(s):  
Plant Growth ◽  
Chlorophyll Content ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Total Biomass ◽  
Total Chlorophyll ◽  
Water Spray ◽  
Benzyl Adenine ◽  
Total Chlorophyll Content ◽  
Different Levels

An experiment was conducted at the Instructional Farm of Rajasthan College of Agriculture, Udaipur during Kharif 2009 and 2010 on medium clay loam soils to assess the influence of different levels and sources of phosphorus fertilization as well as plant growth regulators on productivity of soybean. Twenty seven treatments i.e. three levels (20, 30 and 40 kg P2O5 ha-1) and three sources (Single super phosphate or SSP; phosphorus rich organic manure or PROM and di-ammonium phosphate or DAP) of phosphorus in main plots and three PGRs (water spray, benzyl adenine 50 ppm and NAA 100 ppm) in sub plots of a split plot design having three replications. Results revealed that application of 40 kg P2O5 ha-1 recorded significantly higher pooled yield (q ha-1) of grain (25.95), stover (37.34) and total biomass (63.29); crop growth rate or CGR (g m-2day-1) between 30-60 days after sowing ( DAS)(18.83) and 61-90 DAS (11.96); total chlorophyll content at 45 DAS (2.26%) and 60 DAS (2.80%); Leaf area index(LAI) at 45 DAS (1.56) and 60 DAS (3.44) and green leaves plant-1 at 45 DAS (11.98) and 60 DAS (21.43). Among different phosphorus sources, SSP outperformed DAP and PROM on pooled yield (q ha-1) of grain (25.12), stover (36.23) and total biomass (61.35); CGR (g m-2day-1) between 30-60 DAS (11.22) and 61-90 DAS (10.94); total chlorophyll content at 45 DAS (2.26%) and 60 DAS (2.76%); LAI at 45 DAS (1.51) and 60 DAS (3.42) and green leaves plant-1 at 45 DAS (11.77) and 60 DAS (21.35). Foliar application of NAA recorded significantly higher pooled yield (q ha-1) of grain (24.23), stover (35.73) and total biomass (60.01) than benzyl adenine and water spray. However, variations recorded under NAA and benzyl adenine in CGR, total chlorophyll content, LAI and green leave plant-1at different stages of soybean crop were statistically at par.

scholarly journals USE OF FORCING SOLUTIONS TO STUDY PLANT GROWTH REGULATOR EFFECTS ON VANHOUTTE'S SPIRAEA CULTURED IN VITRO

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124e-1124
Author(s):  
Guochen Yang ◽  
P. E. Read
Keyword(s):  
Plant Physiology ◽  
Plant Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Plant Tissues ◽  
Forcing Solution

Vanhoutte's spiraea has been propagated in vitro using explants from softwood growth of dormant stems forced in a solution containing 200 mg/l 8-hydroxyquinoline citrate (8-HQC) and 2% sucrose (Yang and Read, 1989). Objectives to further utilize this system were to determine the feasibility of applying plant growth regulators (PGR) via the forcing solution to softwood growth from forced dormant stems and to study the resulting influence on in vitro culture. BA and GA3 were placed in the forcing solution at various concentrations, including a zero PGR control. Explants were cultured on Linsmaier and Skoog (LS) medium containing zero PGR or different amounts of BA or thidiazuron (TDZ) or combinations of BA and IAA. Control explants placed on LS medium supplemented with 5uM BA with or without 1 or 5uM IAA, or with 0.5 or 0.75 uM TDZ alone produced the best shoot proliferation. BA in the forcing solution stimulated micropropagation, while GA3 caused less proliferation than explants from control solutions. Forcing solutions containing PGR are useful for manipulating responses of plant tissues cultured in vitro and for studying PGR influence on woody plant physiology.

scholarly journals Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona

2016 ◽  
Vol 8 (2) ◽  
pp. 535-540
Author(s):  
Priyadarshani P. Mohapatra ◽  
V.K. Batra ◽  
Subhash Kajla ◽  
Anil K. Poonia ◽  
N. Manoj Kumar
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Basal Medium ◽  
Potato Cultivar ◽  
Shoot Tips ◽  
Scale Production ◽  
Survival Percentage ◽  
Planting Material ◽  
Micro Propagation

In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.

Effects of growth regulators and controlled atmosphere on stored carrots

1974 ◽  
Vol 82 (2) ◽  
pp. 245-249 ◽  
Author(s):  
M. Abdel-Rahman ◽  
F. M. R. Isenberg
Keyword(s):  
Gibberellic Acid ◽  
Growth Regulators ◽  
Dichlorophenoxyacetic Acid ◽  
Trimethylammonium Chloride ◽  
Controlled Atmosphere ◽  
Benzyl Adenine ◽  
Large Size ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Better Than

SummaryPreharvest sprays with 6-hydroxy-3-(2H)-pyridazinone (MH), or immersion in solutions of MH, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-chloroethylphosphonic acid (ethephon), coumarin or (2-chloroethyl) trimethylammonium chloride (CCC) inhibited both sprouting and rooting of treated carrots during storage at 0 °C whencompared with water-dipped or untreated carrots, while N-6-benzyl-adenine (BA) dips stimulated extensive rooting and sprouting. In contrast, immersion in gibberellic acid (GA3) solutions had very little effect.Storage atmospheres containing 1, 2.5, 5 or 10% oxygen inhibited both sprouting and rooting but increased mould infection. Atmospheres containing 21% (air) or 40% oxygen reduced mould infection, but increased sprouting and rooting of stored carrots. Washing carrots in water before storage did not significantly affect mould infection, while washing in 0.5% clorox increased it. However, washing in water followed by immersion in MH inhibited both sprouting and rooting and also reduced mould infection. ‘Jumbo’ and large-size carrots stored better than medium and small carrots.

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