Formulasi Sediaan Salep Ekstrak Daun Puring (Codiaeum variegatum L.) Dengan Basis Hidrokarbon Dan Larut Air

Daun puring (Codiaeum variegatum L.) merupakan tanaman hias yang banyak digunakan sebagai bahan obat tradisional. Daun puring mengandung senyawa metabolit sekunder diantaranya terpenoida, flavonoida, saponin, alkaloida, dan tannin. Senyawa metabolit flavonoida diketahui memiliki aktivitas antibakteri. Tujuan dari penelitian adalah untuk memformulasikan sediaan salep ekstrak daun puring dan mengetahui pengaruh basis formula terhadap stabilitas fisiknya. Penelitian ini merupakan penelitian eksperimental laboratorium yang dilakukan dengan pengujian stabilitas fisik metode freeze thaw dan sifat fisik salep yang meliputi uji organoleptis, homogenitas, pH, daya lekat, daya sebar, dan viskositas. Data dianalisis secara statistika dengan metode uji ANOVA dan Kruskal walis. Hasil penelitian menunjukkan bahwa ekstrak daun puring dapat diformulasikan menjadi sediaan salep dengan basis hidrokarbon dan basis larut air. Formulasi ekstrak daun puring terhadap basis hidrokarbon dan larut air berpengaruh terhadap stabilitas fisik seperti uji organoleptis, pH, homogenitas, dan daya sebar namun tidak stabil pada uji viskositas dan daya lekat.

Uji Efektivitas Antibakteri Sediaan Salep Ekstrak Daun Puring (Codiaeum variegatum (L.) Rumph. Ex A.Juss) Terhadap Bakteri Staphylococus aureus

Indonesia is one of the rich countries among the plant’s potential medicinal properties is the puring leaf (Codiaem variegatum (L.) Rumph. Ex A.Jus) as an antibacterial. The puring leaves contain flavonoid, steroids, alkaloids, tannins, saponins and fenols. which serve as antibacterial. The use of antibacterial ointments can treat bacterial skin infections. Balm is a half-denser is intended for topical wear on the skin or the mucous membranes. Among the causes of infection is the Staphylococus aureus bacteria. The study aims to test the effectiveness of a suppressive antibacterias, the leaf extract ointment against the Staphylococus aureus bacteria. Extraction methods maceration method use a 96% solution. The research method used was oriental. The extract of the puring leaves used is 2,5%, 5%, 7,5%, 10%, the negative cotrol of the ointment without the extract and the positive control with the gentamicin ointment. Zone research is inhibiting every single formula. Averag buffer zone on formula 1 6.25 mm, formula 2 7.55 mm, formula 3 9.23 mm and formula 4 11.68 mm. Data acquired by the formation of a clear buffer zone around the commonwealth for 24 hours after treatment. The data was analyzed with one way ANOVA continues the post hoc test (Tukey). The result is that partial extract of puring leave can be made into unguent and effective Staphylocous aureus ATCC 25923Keywords: Extract of puring leaves; antibacterial; ointment and staphylococus aureus AbstrakIndonesia merupakan salah satu negara yang kaya akan keaneragaman tanaman yang memiliki potensi sebagai bahan obat, salah satunya yaitu daun puring (Codiaeum variegatum (L.) Rumph. Ex A.Juss) sebagai antibakteri. Daun puring memiliki senyawa flavonoid, steroid, alkaloid, tannin, saponin dan fenol yang berfungsi sebagai antibakteri. Penggunaan salep antibakteri dapat mengobati infeksi kulit yang disebabkan oleh bakteri. Salep merupakan sediaan setengah padat yang ditujukan untuk pemakaian topikal pada kulit atau selaput lendir. Salah satu penyebab infeksi adalah bakteri Staphylococcus aureus. Penelitian ini bertujuan untuk menguji efektivitas antibakteri sediaan salep ekstrak daun puring terhadap bakteri Staphylococus aureus. Metode ekstraksi menggunakan metode maserasi dengan pelarut 96%. Metode penelitian yang digunakan yaitu sumuran. Konsentrasi ekstrak daun puring yang digunakan adalah 2,5%, 5%, 7,5%, 10%, kontrol negatif yaitu salep tanpa ekstrak dan kontrol positif dengan salep gentamicin. Hasil penelitian zona hambat setiap formula berbeda. Rata-rata zona hambat pada formula 1 6,25 mm, formula 2 7,55 mm, formula 3 9,23 mm dan formula 4 11,68 mm. Data yang diperoleh dengan terbentuknya zona hambat bening di sekeliling sumuran selama 24 jam setelah perlakuan. Data dianalisa dengan one way ANOVA dilanjutkan uji post Hoc (Tukey). Kesimpulannya Ekstrak daun puring bisa dibuat sediaan salep dan ekstrak daun puring efektif terhadap bakteri Staphylococus aureus ATCC 25923Kata Kunci: Ekstrak daun puring; antibakteri; salep dan staphylococus aureus

Uji Aktivitas Antikolesterol Ekstrak Daun Puring (Codiaeum variegatum (L.) Rumph. Ex. A.Juss) Secara In Vitro

Cholesterol is an essential building material for the body to synthesize important substances such as cell membranes and insulation materials around nerve fiber as well as genital hormones and kidney of vitamin D and bile acids. Puring plants iclusive varieties of flowering plants who most of society interest because have varied colour leaf and have compound secondary metabolic substances of flavonoid, phenolic, triterpenoid, steroid and alkaloids. The purpose of this study was to determine the anticholesterol activity of puring leaves in vitro. The method used in vitro with Lieberman-Burchad reagent using a UV-Vis spectrophotometer measuring device at a wavelength of 665.0 nm. The concentration series used are 100; 200; 300; 400 and 500 ppm. The results showed that at a concentration of 500 ppm it can decrease cholesterol levels by 52.20% and The EC50 value obtained was 449.87 g/mL. The increase in each extract concentration showed an increase in the percent decrease in cholesterol levels.Keywords: cholesterol; puring leaf; lieberman-burchard AbstrakKolesterol merupakan bahan bangun esensial bagi tubuh untuk sintesis zat-zat penting, seperti membran sel dan bahan isolasi sekitar serat saraf, begitu pula hormon kelamin dan anak ginjal, vitamin D, serta asam empedu. Tanaman puring termasuk jenis tanaman hias yang banyak diminati masyarakat karena memiliki warna daun yang beragam dan memiliki kandungan senyawa metabolit sekunder berupa flavonoid, fenolik, triterpenoid, steroid dan alkaloid. Tujuan penelitian ini untuk mengetahui aktivitas antikolesterol pada daun puring secara in vitro. Metode yang digunakan secara in vitro dengan pereaksi Lieberman-Burchad menggunakan alat pengukur spektrofotometer UV-Vis pada panjang gelombang 665,0 nm. Seri konsentrasi yang digunakan yaitu 100; 200; 300; 400 dan 500 ppm. Hasil penelitian menunjukkan pada konsentrasi 500 ppm dapat menurun kadar kolesterol sebesar 52,20% dan nilai EC50 yang didapatkan sebesar 449,87 µg/mL. Peningkatan setiap konsentrasi ekstrak menunjukan peningkatan persen penurunan kadar kolesterol.Kata kunci: daun puring; kolesterol; Lieberman-burchard

The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro

Production problems related to aquaculture cannot be separated from diseases that can cause death in fish. Efforts to overcome the bacterial disease Edwardsiella tarda can be done by using natural materials such as the leaves of croton (Codiaeum variegatum). This study aimed to analyze the content of active compounds contained in croton leaf extract which is antibacterial against E. tarda bacteria. Inhibition test was performed by dilution method Minimum Inhibition Concentration (MIC test) and agar diffusion (disc test). The MIC test used five concentrations: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L, and 1000 mg/L on Tryptone Soy Broth (TSB) media, while the disc test used five doses of croton leaf extract, namely: A (100 mg/L), B ( 200 mg/L), C (300 mg/L), D (400 mg/L) and E (500 mg/L), 2 controls (positive and negative), and three replications on Tryptone Soy Agar (TSA) media and incubated for 2x24 h. The positive control used chloramphenicol (5 mg/L) antibiotic. Croton leaf extract contains several compounds, namely saponins, flavonoids, tannins, steroids, and polyphenols, which are antibacterial and could kill the test bacteria with the highest average inhibition zone results obtained at a dose of E (500 mg/L) of 6.34 mm, while the lowest results were obtained at dose A (100 mg/L) of 3.12 mm.

Assessment of lectinic activity potentials in extracts of some tropical Euphorbiaceace

Lectins bind a variety of cells having cell surface glycoprotein or glycolipid, such as erythrocytes, leukemic cells, yeast and several types of bacteria. Several specificity groups have been identified such as mannose, galactose, N-acetyl glucoseamine, N-acetyl galactoseamine, L-fucose and N-acetyl neuraminic acid. The presence of two or more binding sites for each Lectin molecule allows the agglutination of many cell type. Sixteen (16) species of some tropical Euphorbiaceae plants were assessed for the presence of Lectins. The leaves of Acalypha torta, Acalypha wiskesiana, Acalypha hispida, Codiaeum variegatum, Euphorbia milli, Euphorbia pulcherima, Jatropha curcas and Jatropha gossypifola; the seeds of Croton tiglium, Ricinus comminus and Tetracarpidium conophorum; the stem of Euphorbia tirucalli and the tubers of Manihot esculenta (Cassava, vitamin A variety), Manihot esculenta (cassava, NR 8082 variety), Manihot esculenta (Cassava, TMX 419 variety), Manihot esculenta (Cassava, TMX 4(2) 1425 variety) were used for sourcing the Lectins. A. torta, A. wiskesiana, C. variegatum, C. tiglium, E. milli, E. pulcherima, E. tirucalli, J. curcas, J. gossypifola, R. comminus and T. conophorum agglutinated pooled washed human ABO cells in saline (direct haemagglutination) while A. hispida and the four varieties of M. esculenta showed no agglutination reaction. E. pulcherima showed specificity for B cells only while E. tirucalli showed specificity for O cells only, hence could be rightly indicated by referring to them as anti-B Ep and anti-H Et Lectins respectively (where Ep=Euphorbia pulcherima and Et= Euphorbia tirucalli). However A. torta and T. conophorum cross-reacted with pooled washed ABO cells in differing strengths and when standardized, showed that A. torta at a titre of 16 reacted specifically with O cells and T. conophorum at a titre of 128 reacted specifically with B cells. Based on this, these Lectins could be indicated as anti-H At and anti-B Tc respectively (Where At= Acalypha torta and Tc= Tetracarpidium conophorum). The protein content of the crude extracts of the sixteen (16) species were also assayed using Biuret protein assay method and the results revealed that there are no relation or association between the quantity of protein content and agglutination patterns of the extracts. This research has therefore succeeded in revealing presence of Lectinic properties in extracts of some tropical Euphorbiaceae.

Codiaeum variegatum (garden croton).

A datasheet on Codiaeum variegatum covering, as an economically important tree, its taxonomy, importance, silviculture, distribution, biology and ecology, uses, products and pests.

Antipyretic effect of ethanol extract of plant leaves of garden croton (Codiaeum variegatum) in male mice

Codiaeum variegatum (L.) is known to contain bioactive compounds that have many pharmacological properties such as abortifacient, antiamoebic, antibacterial, anticancer, antifungal, and antioxidant. This study aims to reveal whether the ethanol fraction of leaf extract of this plant has antipyretic activity. The male mice (n=25) with a weigh range of 20 – 30 g were divided into five groups (5 mice each). To make pyrexia condition, the animals were induced with DPT-HB-HIB vaccine. The ingredients given to the animals were as follows: distilled water as the negative control; standard drug (paracetamol) as positive control; and three different dosage of croton leaves extract namely: 71.5, 143 and 286 mg/kg BW. The body temperature of the mice was measured using a digital thermometer through the rectal opening every 30 minutes for 180 minutes. The results showed that ethanol fraction of croton plant leaf extract at the dosage of 143 and 286 mg/kg BW statistically have the same effect as paracetamol in decreasing body temperature of mice. Therefore, it can be concluded that plant extract of garden croton is potential to be used as antipyretic remedy.