EFFECT OF CULTIVARS, AUXINS AND ACTIVATED CHARCOAL ON IN VITRO ROOTS FORMATION OF STRAWBERRY PLANTLETS

Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

Download Full-text

The Effect of Activated Charcoal Supplemented Media to Browning of In Vitro Cultures of Piper species

Biologia Plantarum ◽

10.1023/a:1002729032527 ◽

2000 ◽

Vol 43 (2) ◽

pp. 297-299 ◽

Cited By ~ 21

Author(s):

K. Madhusudhanan ◽

B.A. Rahiman

Keyword(s):

Activated Charcoal ◽

In Vitro Cultures

Download Full-text

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n4p1779 ◽

2016 ◽

Vol 37 (4) ◽

pp. 1779 ◽

Cited By ~ 1

Author(s):

Herick Fernando de Jesus Silva ◽

Simone Abreu Asmar ◽

Rayssa Camargo de Oliveira ◽

Berildo De Melo ◽

José Magno Queiroz Luz ◽

...

Keyword(s):

Activated Charcoal ◽

Large Scale ◽

Culture Media ◽

Germination Rate ◽

Woody Species ◽

Chlorophyll Contents ◽

Fruit Species ◽

In Vitro Establishment ◽

Dipteryx Alata

The barueiro (Dipteryx alata Vog.) is a native fruit species of the Cerrado ecoregion that has multiple uses. It is a wild species, and its cultivation is difficult. Furthermore, it is threatened with extinction. Plant tissue culture is a major tool for the conservation of germplasm, as well as a means of propagating high-quality seedlings on a large scale. However, this technique has not been used with barueiro, although it might provide valuable contributions to the process of barueiro domestication. The most popular method of cultivation is the use of the Murashige and Skoog medium (MS), which is considered one of the most nutritionally complete media. Woody plant medium (WPM) is indicated for the propagation of woody species, but there are no reports of its use for barueiro cultivation. Woody plants tend to have problems with rust in vitro during the establishment phase. Activated charcoal acts as an adjuvant for the adsorption of phenolic compounds, mitigating its effects in the medium. Thus, the objective of this study was to test four activated charcoal doses (0, 2, 4 and 6 g L-1) and three culture media: MS, WPM, and AA (over water agar) in the in vitro establishment of barueiro. The experimental design was a completely randomised (DIC), 4 × 3 factorial design with three replications. At 60 days after inoculation, the explants were evaluated for dry matter, fresh weight, stem diameter, shoot length, number of leaves, longest root length, germination rate, and chlorophyll contents. The MS medium supplemented with 3,0 g L-1 activated charcoal appeared to be the best for in vitro establishment of barueiro.

Download Full-text

In Vitro Adsorption of Oxalic Acid and Glyoxylic Acid onto Activated Charcoal, Resins and Hydrous Zirconium Oxide

The International Journal of Artificial Organs ◽

10.1177/039139888200500110 ◽

1982 ◽

Vol 5 (1) ◽

pp. 33-36 ◽

Cited By ~ 1

Author(s):

R. Scholtens ◽

J. Scholten ◽

H.W.M. de Koning ◽

J. Tijssen ◽

H.W.M. ten Hoopen ◽

...

Keyword(s):

Oxalic Acid ◽

Activated Charcoal ◽

Zirconium Oxide ◽

Ionic Composition ◽

Plasma Concentrations ◽

Glyoxylic Acid ◽

Primary Hyperoxaluria ◽

Hydrous Zirconium Oxide ◽

Hydrous Zirconium

Patients suffering from primary hyperoxaluria show elevated plasma concentrations of oxalic acid and glyoxylic acid. The in vitro adsorption of these compounds onto activated charcoal, a series of neutral and ion exchange resins and onto hydrous zirconium oxide has been investigated. Hydrous zirconium oxide was the most effective sorbent studied for the removal of both oxalic acid and glyoxylic acid. In batch experiments, the zirconium oxide was capable of binding 5.5 μ mol oxalic acid and 8 μ mol glyoxylic acid per gram sorbent using 0.5 gram sorbent and 50 ml of solutions with initial concentrations of 100 μ mol. L−1 and an ionic composition resembling that of plasma. Recirculation of 2 L of the same solutions through 12 gram of a mixture of hydrous zirconium oxide and alumina for 6 hours at a flow rate of 12 ml.min−1, resulted in a final concentration of 70 μ mol. L−1 of oxalic acid and 50 μ mol. L−/ of glyoxylic acid.

Download Full-text

Studies of Adsorbents for Hemoperfusion in Artificial Liver Support. I. Preparation and in Vitro Studies of Cross-Linked Agarose Beads Entrapped Activated Charcoal (CAAC)

The International Journal of Artificial Organs ◽

10.1177/039139888100400411 ◽

1981 ◽

Vol 4 (4) ◽

pp. 200-204 ◽

Cited By ~ 23

Author(s):

C.X. Xu ◽

X.J. Tang ◽

Z. Niu ◽

Z.M. Li

Keyword(s):

Thermal Stability ◽

Blood Flow ◽

High Temperature ◽

Mechanical Strength ◽

Activated Charcoal ◽

Artificial Liver ◽

Liver Support ◽

Artificial Liver Support ◽

Agarose Beads

A new method for the preparation of the cross-linked agarose beads entrapped activated charcoal (CAAC) is reported. Since the agarose-encapsulated adsorbents reported elsewhere cannot stand high temperature for sterilization, the CAAC has the advantage of thermal stability to withstand autoclave at 121°C. for ½ hour without breaking up or melting. A further advantage of CAAC is that the adsorbent has a much better consistency with good mechanical strength and elasticity, so that it can be formed into beads of a diameter less than 1 mm. This will not only give a better adsorption capacity than larger beads, but can also assure a better blood flow than soft beads which usually interfere in hemoperfusion due to compacting and sludging. Preliminary investigations indicate that the CAAC is relatively hemocompatible.

Download Full-text