In vitro assessment of some deodorant ingredients and determination of malodor inhibiting potentials of ascorbic acid, orlistat and mastic gum

Although methods exist to readily determine the particle size distribution (PSD) of an active pharmaceutical ingredient (API) before its formulation into a final product, the primary challenge is to develop a method to determine the PSD of APIs in a finished tablet. To address the limitations of existing PSD methods, we used hot-stage microscopy to observe tablet disintegration during temperature change and, thus, reveal the API particles in a tablet. Both mechanical and liquid disintegration were evaluated after we had identified optimum milling time for mechanical disintegration and optimum volume of water for liquid disintegration. In each case, hot-stage micrographs, taken before and after the API melting point, were compared with image analysis software to obtain the PSDs. Then, the PSDs of the APIs from the disintegrated tablets were compared with the PSDs of raw APIs. Good agreement was obtained, thereby confirming the robustness of our methodology. The availability of such a method equips pharmaceutical scientists with an in vitro assessment method that will more reliably determine the PSD of active substances in finished tablets.

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Utilization of a new gold/Schiff-base iron(iii) complex composite as a highly sensitive voltammetric sensor for determination of epinephrine in the presence of ascorbic acid

RSC Advances ◽

10.1039/c6ra22028b ◽

2016 ◽

Vol 6 (104) ◽

pp. 101888-101899 ◽

Cited By ~ 5

Author(s):

Adam Gorczyński ◽

Maciej Kubicki ◽

Klaudia Szymkowiak ◽

Teresa Łuczak ◽

Violetta Patroniak

Keyword(s):

Ascorbic Acid ◽

Schiff Base ◽

Schiff Base Complex ◽

Voltammetric Sensor ◽

Base Complex ◽

Highly Sensitive

A new voltammetric sensor based on an iron(iii) Schiff-base complex/Au composite is synthesized and applied for the in vitro detection of epinephrine.

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DETERMINATION OF THE HYDROLYTIC ACTIVITY OF WHOLE SALIVA USING CHITOSAN ASCORBATE AS A SUBSTRATE

Progress on Chemistry and application of Chitin and its Derivatives ◽

10.15259/pcacd.23.009 ◽

2018 ◽

Vol XXIII ◽

pp. 97-102

Author(s):

Barbara Kochańska ◽

Mirela Łukaszewska ◽

Jolanta Ochocińska

Keyword(s):

Ascorbic Acid ◽

In Vitro Study ◽

Hydrolytic Activity ◽

Vitro Study ◽

Whole Saliva ◽

Unstimulated Whole Saliva

The aim of this work was to evaluate of hydrolytic activity of whole saliva using chitosan ascorbate as a substrate. In this aim, the concentrations of N-acetyl-D-glucosamine were determined in saliva before addition of chitosan ascorbate, directly after addition and during incubation with chitosan ascorbate by 20 hrs. In this in vitro study were used sterile chitosan ascorbate in the form of powder. Chitosan was obtained from krill chitin. The ratio of ascorbic acid to chitosan was 1:1. The unstimulated whole saliva showed the hydrolytic activity in the presence of the chitosan ascorbate as a substrate.

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In-vitro Assessment of Effectiveness and Photostability Avobenzone in Cream Formulations by Combination Ethyl Ascorbic acid and alpha Tocopherol Acetate

Journal of Applied Pharmaceutical Science ◽

10.7324/japs.2015.50611 ◽

2015 ◽

pp. 070-074 ◽

Cited By ~ 2

Author(s):

Marline Abdassah ◽

Ratih Aryani ◽

Emma Surachman ◽

Muchtaridi Muchtaridi

Keyword(s):

Ascorbic Acid ◽

Alpha Tocopherol ◽

Tocopherol Acetate ◽

In Vitro Assessment

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Nutritional and Anti Oxidant Potential of Commonly Growing Plant Foods from Southern India

Defence Life Science Journal ◽

10.14429/dlsj.5.13959 ◽

2020 ◽

Vol 5 (1) ◽

pp. 31-37

Author(s):

C. Rajendran ◽

S. KirtanaKirtana ◽

Rashmi Venkatesh ◽

Pooja Yadav ◽

Anilakumar Kandankath Raghavan

Keyword(s):

Ascorbic Acid ◽

Nutritional Composition ◽

Water Soluble ◽

Food Supplementation ◽

Nutrient Deficiencies ◽

Fruit Pulp ◽

Mineral Contents ◽

Curry Leaves

Certain varieties of plants are abundantly available with rich in nutrients, but the complete sets of nutritional composition studies have not been carried out. Among them, the matured leaves of Murrya keonigii; seeds and leaves of Moringa oleifera, and fruit pulp of Aegle marmelos were chosen. The proximate analysed for above samples showed that the moisture varies from 2.4% -8.2%, crude proteins from 18.75%- 34.57 %, fat content from 5.6% -19.6%, ash content from 3.87%-15.6% and carbohydrate from 28.65% - 41.23%, respectively. Estimation of mineral contents revealed that the leaves and seeds of moringa had higher concentrations than others. Determination of total antioxidant (total flavonoid) was higher (63.550 μg (RU)/ml of samples) in matured curry leaves than others. Determination of in vitro antioxidant activity with FRAP revealed highest activity in curry leaves (174.5μg) to a least activity with seeds of moringa (45.725 μg), whereas the DPPH revealed highest activity for fruit pulp of bael (1680.6μg ascorbic acid) to the least in leaves of moringa (263.15 μg ascorbic acid equivalent/mg). The ABTS showed IC50 value of 210.52 ±0.5774 for curry leaf, 487.8 ±0.3347 and 205.36 moringa seeds and leaves and 513.24 IC50 μg for pulp of bael fruit. Quantitative analysis of water soluble vitamins such as thiamine (B1) varied from 2.172 -5.558 mg; riboflavin (B2) from 2.201 -11.354 mg; pyridoxine (B6) from 4.608 -194.001 mg; biotin (B7) from 36.864 -153.027 mg, respectively. Further, the vitamin C (ascorbic acid) varied from 6.733 -23.142 mg /100g of oven dried samples, respectively. Therefore, the above foods are well intended to be included in routine diet regime and may also be willing to utilise for food supplementation with other suitable diets to manage the conditions such as malnutrition and nutrient deficiencies.

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Determination of eight carbonyl compounds in aerosols trapped in phosphate buffer saline solutions to support in vitro assessment studies

Talanta ◽

10.1016/j.talanta.2018.02.048 ◽

2018 ◽

Vol 184 ◽

pp. 42-49 ◽

Cited By ~ 2

Author(s):

Roberto Buratto ◽

Daniela Correia ◽

Monique Parel ◽

Maude Crenna ◽

Mickaël Bilger ◽

...

Keyword(s):

Phosphate Buffer ◽

Carbonyl Compounds ◽

Phosphate Buffer Saline ◽

In Vitro Assessment ◽

Saline Solutions

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SUN PROTECTION FACTOR DETERMINATION STUDIES OF SOME SUNSCREEN FORMULATIONS USED IN COSMETICS FOR THEIR SELECTION

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v8i5-s.1924 ◽

2018 ◽

Vol 8 (5-s) ◽

pp. 149-151 ◽

Cited By ~ 1

Author(s):

Masheer Ahmed Khan

Keyword(s):

Sun Protection ◽

Protection Factor ◽

Dynamic View ◽

Laboratory Measure ◽

In Vitro Assessment ◽

Sun Protection Factor ◽

Uv Dose ◽

Selection Of

The study involves determination of sun protection factor (SPF) values of some sunscreen formulations for their use in cosmetics. The Sun Protection Factor (SPF) is a very popular instrument in the marketing of sunscreens. Sun protection factor is a laboratory measure of the effectiveness of sunscreen, the higher the SPF, the more protection a sunscreen offers against the ultraviolet radiations causing sunburn. It is often not understood how sunscreens work and where the limitations of the SPF are. A lot of aspects of the SPF are confusing, e.g. the race for higher and higher numbers, the effect on SPF when less sunscreen is applied and if sunscreen should be used at all because they may block the Vitamin D synthesis. The study explains how sunscreens work, how the SPF is determined and where the limitations of the current methods exist. The dynamic view of 'UV radiation applied' and the 'UV dose transmitted' through the sunscreen onto the skin as well as onto a substrate in vitro help in the understanding and are also promising approaches in the in vitro assessment. The study is helpful in selection of some sunscreens formulations used in cosmetics with better safety and high SPF values. Keywords: Sun Protection Factor, SPF, Sunscreens

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