scholarly journals Determination of eight carbonyl compounds in aerosols trapped in phosphate buffer saline solutions to support in vitro assessment studies

Talanta ◽  
2018 ◽  
Vol 184 ◽  
pp. 42-49 ◽  
Author(s):  
Roberto Buratto ◽  
Daniela Correia ◽  
Monique Parel ◽  
Maude Crenna ◽  
Mickaël Bilger ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Carbonyl Compounds ◽  
Phosphate Buffer Saline ◽  
In Vitro Assessment ◽  
Saline Solutions

scholarly journals Development and validation of UV spectrophotometric method for quantitative estimation of Promethazine HCl in phosphate buffer saline pH 7.4

2013 ◽  
Vol 2 (8) ◽  
pp. 141-142
Author(s):  
Dwi Nurahmanto
Keyword(s):  
Phosphate Buffer ◽  
Quantitative Estimation ◽  
Pharmaceutical Journal ◽  
Analytical Validation ◽  
Phosphate Buffer Saline ◽  
Validation Procedure ◽  
Development And Validation ◽  
Light Absorbance

A new, sensitive, rapid, simple, specific and economical procedure has been developed for determination Promethazine HCl in phosphate buffer saline pH 7.4. The purpose of this analytical validation procedure is to determine a process of assessment and to validate it by laboratory experiments to prove that the method meets the minimum standard for laboratory use. This analytical method for the determination of Promethazine HCl in phosphate buffer saline pH 7.4 can be used to estimate the amount of promethazine HCl penetrated and dissolved in the blood vessels in vitro by penetration study. The method is based on the ultraviolet light absorbance at 251 nm which is the maximum wavelength of the concerned drug. This method can be succesfully applied for determination of drug in phosphate buffer saline pH 7.4 . The results of the analysis have been validated statistically and by recovery studies.DOI: http://dx.doi.org/10.3329/icpj.v2i8.15589 International Current Pharmaceutical Journal, July 2013, 2(8): 141-142

scholarly journals In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch

2017 ◽  
Vol 10 ◽  
pp. S3699-S3706 ◽  
Author(s):  
Laila Jafri ◽  
Samreen Saleem ◽  
Ihsan-ul-Haq ◽  
Nazif Ullah ◽  
Bushra Mirza
Keyword(s):  
Polyphenolic Compounds ◽  
Antioxidant Potential ◽  
In Vitro Assessment

scholarly journals Hot-Stage Microscopy for Determination of API Particles in a Formulated Tablet

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Michal Šimek ◽  
Veronika Grünwaldová ◽  
Bohumil Kratochvíl
Keyword(s):  
Active Pharmaceutical Ingredient ◽  
Assessment Method ◽  
Hot Stage Microscopy ◽  
Tablet Disintegration ◽  
Before And After ◽  
In Vitro Assessment ◽  
Active Substances ◽  
Good Agreement

Although methods exist to readily determine the particle size distribution (PSD) of an active pharmaceutical ingredient (API) before its formulation into a final product, the primary challenge is to develop a method to determine the PSD of APIs in a finished tablet. To address the limitations of existing PSD methods, we used hot-stage microscopy to observe tablet disintegration during temperature change and, thus, reveal the API particles in a tablet. Both mechanical and liquid disintegration were evaluated after we had identified optimum milling time for mechanical disintegration and optimum volume of water for liquid disintegration. In each case, hot-stage micrographs, taken before and after the API melting point, were compared with image analysis software to obtain the PSDs. Then, the PSDs of the APIs from the disintegrated tablets were compared with the PSDs of raw APIs. Good agreement was obtained, thereby confirming the robustness of our methodology. The availability of such a method equips pharmaceutical scientists with an in vitro assessment method that will more reliably determine the PSD of active substances in finished tablets.

scholarly journals Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium

2015 ◽  
Vol 15 (2) ◽  
pp. 72-78
Author(s):  
Rini Widyastuti ◽  
Siti Darodjah Rasad
Keyword(s):  
Phosphate Buffer ◽  
Bovine Oocyte ◽  
Oocyte Maturity ◽  
Phosphate Buffer Saline ◽  
Bovine Oocytes ◽  
Meiotic Competence

(Nuclear maturity of bovine oocyte after 24 hours ovary preservation)ABSTRACT. The objective of the research was to investigate  their meiotic competence or nuclear maturity of bovine oocytes maturity in vitro after 24 hour preservation on 5°C. Oocytes  were collected by slicing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) homogenous ooplasm  were cultured in maturity medium at 38°C in humidified atmosphere of 5% CO2 incubator. After 24 hours, oocytes stained for nuclear maturity’s evaluation. The proportion of oocytes at metaphase II (MII) was significantly difference (P 0.05) on oocytes that 24 hours preservated   (44.21 ± 3.04%) vs oocytes from fresh ovary (73.97 ± 9.32%) (P0.05). These results indicated that 24 hour’s preservation  bovine’s ovary on 50C  cause  decreases of nuclear oocyte maturity.

scholarly journals Investigation of surface topography of different root-end filling materials: An in vitro study

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Mine Koruyucu ◽  
Hazal Özcan ◽  
Merve Bayram ◽  
Abdullah Burak Cankaya ◽  
Nurullah Keklikoglu ◽  
...  
Keyword(s):  
Surface Topography ◽  
Normal Saline ◽  
Phosphate Buffer ◽  
Saline Solution ◽  
In Vitro Study ◽  
Phosphate Buffer Saline ◽  
Filling Materials ◽  
Retrograde Filling ◽  
Group B

Aim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials.Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group dimidiated two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling.Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plague. A2,B2,C2 samples have more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2.Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution was more than normal saline solution for all groups as a hydroxiapatite crystals.  

Validation of RP-HPLC Method for Determination of Omeprazole in Dissolution Media and Application to Study in-vitro Release from Solid-SNEDDS

2021 ◽  
Vol 17 ◽  
Author(s):  
Suhair S. Al-Nimry ◽  
Khouloud A. Alkhamis ◽  
Bashar M. Altaani
Keyword(s):  
Dissolution Rate ◽  
Phosphate Buffer ◽  
Water Solubility ◽  
In Vitro Release ◽  
Hplc Method ◽  
In Vitro Dissolution ◽  
Limit Of Quantification ◽  
Rp Hplc

Background: Omeprazole has poor water solubility, is unstable in acidic solutions, and undergoes first pass metabolism which results in lowering its bioavailability. A solid Self-Nano Emulsifying Drug Delivery System (SNEDDS) was previously prepared to enhance its dissolution. Objective: Development and validation of a RP-HPLC method with UV detection for the determination of omeprazole in 0.1N HCl and in 0.01 M phosphate buffer (pH 7.4). Methods: Validation was according to the ICH Q2 (R1) guidelines in terms of linearity, accuracy and precision, lower limit of quantification, sensitivity, specificity, and robustness. The developed and validated method was used to study the in-vitro dissolution of the drug from the solid-SNEDDS, commercial products and of the unprocessed drug. The dissolution was studied in 500 ml of 0.1N HCl during the first 2 hours, and 900 mL of 0.01 M phosphate buffer (pH 7.4) during the last hour (37 ± 0.5 oC and 100 rpm). Results: The method was linear in the range 1-50 μg/ml, accurate and precise as indicated by the ANOVA test. It was specific to the drug and the pharmaceutical excipients did not affect the determination of its concentration. The method was robust to small changes in pH, composition, and flow rate of the mobile phase. The dissolution rate of omeprazole from the Solid-SNEDDS was faster than that from two commercial dosage forms and than the dissolution rate of the unprocessed drug. Conclusion: The method met the acceptance criteria and was applied successfully in studying the rate of dissolution of the drug.

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