Distribution of 14C-labelled Atrazine, Methoxychlor, Glyphosate, and Bisphenol-A in Goldfish Studied by Whole-Body Autoradiography (WBARG)

2008 ◽  
Vol 43 (4) ◽  
pp. 265-274 ◽  
Author(s):  
Claude Rouleau ◽  
Jagmohan Kohli
Keyword(s):  
Bisphenol A ◽  
Carassius Auratus ◽  
Environmental Risks ◽  
Whole Body ◽  
Intestinal Lumen ◽  
Whole Body Autoradiography ◽  
Goldfish Carassius Auratus ◽  
Uveal Tract ◽  
Target Organs ◽  
Freeze Dried

Abstract Nonpersistent contaminants represent thousands of chemicals used as pesticides, pharmaceuticals, personal care products, additives, etc. Because of this diversity, the assessment of the environmental risks they may pose for the environment represents a formidable task. Identification of target organs is key information needed to orient further research on newlyinvestigated organic xenobiotics. We used whole-body autoradiography to visualize the distribution of 14C-labelled atrazine, methoxychlor, glyphosate, and bisphenol-A in goldfish (Carassius auratus) and identify target organs. Fish were exposed for 2 days (glyphosate and bisphenol-A) and 7 days (atrazine and methoxychlor) to the radiolabelled compounds at a concentration of 15 nM. They were then frozen, embedded in carboxymethylcellulose gel, 20-μm-thick cryosections were collected, freeze-dried, and exposed to phosphor screens to visualize the tissue distribution of radioactivity. Goldfish did not accumulate glyphosate. The three other compounds were accumulated, mostly in the gall bladder. Nevertheless, unforeseen accumulation sites were observed; atrazine accumulated in the uveal tract of the eye, high levels of radioactivity were found in the cerebrospinal fluid of goldfish exposed to methoxychlor, and an important accumulation of bisphenol-A was seen in urine, oral mucosa, esophagus, and intestinal lumen. The potential toxicological consequences of the accumulation of these chemicals at very specific locations within the fish body are discussed and further research suggested.

Whole body autoradiography and microautoradiography in eels after intra-arterial administration of 125I-labeled eel ANP

1996 ◽  
Vol 271 (4) ◽  
pp. R926-R935 ◽  
Author(s):  
H. Sakaguchi ◽  
H. Suzuki ◽  
H. Hagiwara ◽  
H. Kaiya ◽  
Y. Takei ◽  
...  
Keyword(s):  
Binding Sites ◽  
Head Kidney ◽  
Dorsal Aorta ◽  
Whole Body ◽  
Whole Body Autoradiography ◽  
Target Organs ◽  
Secondary Lamellae ◽  
Ventral Aorta ◽  
Interrenal Cells ◽  
Kidney Liver

125I-labeled eel atrial natriuretic peptide (ANP) was administered into the ventral or dorsal aorta of freshwater (FW) and seawater (SW) eels, Anguilla japonica, and the major target organs were explored by whole body autoradiography. Localization of the ANP binding in the target organs was also examined at tissue and cell levels by microautoradiography using tissue sections. Whole body autoradiography revealed that the specific label was accumulated predominantly in the gill, with lesser amounts in the atrium, kidney, liver, and urinary bladder. Autoradiographic grains were most dense in the secondary lamellae of the gill, particularly on the side of the efferent filamental artery. Other binding sites in target tissues were the glomerulus of the kidney, epicardium and endocardium of the atrium, bile duct/blood vessels of the liver, and interrenal cells of the head kidney. There was no difference in the distribution and density of grains between injections into the ventral aorta and dorsal aorta, although, in the former, injected 125I-labeled eel ANP passes through the gill before reaching peripheral target tissues. There was a tendency for downregulation of ANP binding sites in SW eels, especially in the gill. These results show that specific ANP binding sites are present in organs that are implicated in osmoregulation and cardiovascular regulation in eels and further suggest that the number of ANP binding sites varies according to changes in the environmental salinity.

The heart of the adult goldfish Carassius auratus as a target of Bisphenol A: a multifaceted analysis

2021 ◽  
Vol 269 ◽  
pp. 116177
Author(s):  
Mariacristina Filice ◽  
Serena Leo ◽  
Rosa Mazza ◽  
Daniela Amelio ◽  
Filippo Garofalo ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Carassius Auratus ◽  
Goldfish Carassius Auratus ◽  
Multifaceted Analysis

scholarly journals Whole-body amino acid composition of adult fancy ranchu goldfish (Carassius auratus)

2011 ◽  
Vol 106 (S1) ◽  
pp. S110-S112 ◽  
Author(s):  
Donna L. Snellgrove ◽  
Lucille G. Alexander
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Compositional Data ◽  
Essential Amino Acids ◽  
Performic Acid ◽  
Whole Body ◽  
Acid Oxidation ◽  
Farmed Fish ◽  
Goldfish Carassius Auratus ◽  
Freeze Dried

Aqua feeds should be formulated to provide complete and balanced nutrition to achieve optimal health and growth in fish, including adequate levels of essential amino acids (EAA). There are few or no data relating to the EAA requirements for ornamental fish species, with the majority of quantitative data for these nutrients being available for commercially farmed fish. The determination of EAA requirements is usually established through dose–response studies, which can be costly and time consuming, especially if determining the requirement for many amino acids (AA). An alternative method for predicting the EAA of fish, which is also relatively fast and inexpensive, is the assessment of whole-body AA composition. A total of eight goldfish with a mean wet weight of 34·2 (sem 1·4) g were obtained as a result of a routine cull by breeders. The fish were freeze-dried and AA was content analysed by hydrolysis or performic ‘acid’ oxidation. EAA values ranged between 0·97 (sem 0·02) for tryptophan and 7·9 (sem 0·14) for lysine (g/100 g AA). Compositional data were also used to estimate the essential amino acid ratios of these fish. The findings are in agreement with those for juvenile common goldfish, suggesting that there are no differences in whole-body AA composition between juvenile and adult, or fancy and common goldfish. However, these indices do not provide a quantitative total amount of each AA required by the fish, but can be used proportionally to provide guidelines to formulate diets for ornamental species.

Effects of bisphenol A and light conditions on the circadian rhythm of the goldfish Carassius auratus

2017 ◽  
Vol 49 (4) ◽  
pp. 502-514 ◽  
Author(s):  
Ji Yong Choi ◽  
Jong Ryeol Choe ◽  
Tae Ho Lee ◽  
Cheol Young Choi
Keyword(s):  
Circadian Rhythm ◽  
Bisphenol A ◽  
Carassius Auratus ◽  
Light Conditions ◽  
Goldfish Carassius Auratus

SEM of Hepatocytes and Biliary Passages in Goldfish Liver

1977 ◽  
Vol 35 ◽  
pp. 556-557
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Carassius Auratus ◽  
Cell Layer ◽  
Three Dimensional ◽  
Cell Types ◽  
Biliary System ◽  
Fish Food ◽  
Commercial Fish ◽  
Goldfish Carassius Auratus ◽  
Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

Induction of Gynogenesis and Androgenesis in Goldfish Carassius auratus (var. oranda)

2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas
Keyword(s):  
Carassius Auratus ◽  
Goldfish Carassius Auratus
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