Inactivation of health-related microorganisms in water using UV light-emitting diodes

2019 ◽  
Vol 19 (5) ◽  
pp. 1507-1514 ◽  
Author(s):  
Kumiko Oguma ◽  
Surapong Rattanakul ◽  
Mie Masaike
Keyword(s):  
Spectral Sensitivity ◽  
Legionella Pneumophila ◽  
Light Emitting Diodes ◽  
Uv Light ◽  
Feline Calicivirus ◽  
Response Curves ◽  
K Value ◽  
Light Emitting ◽  
Uv Led ◽  
Uv Leds

Abstract UV light-emitting diodes (UV-LEDs) offer various wavelength options, while microorganisms have spectral sensitivity, or so-called action spectra, which can be different among species. Accordingly, matching properly the emission spectra of UV-LEDs and the spectral sensitivity of microorganisms is a reasonable strategy to enhance inactivation. In this study, UV-LEDs with nominal peak emissions at 265, 280 and 300 nm were applied to pathogens including Legionella pneumophila, Pseudomonas aeruginosa, Vibrio parahaemolyticus and feline calicivirus, in comparison with indicator species including Escherichia coli, Bacillus subtilis spores, bacteriophage Qβ and MS2. The results indicated that, for all species tested, 265 nm UV-LED was highest in the fluence-based inactivation rate constant k, followed by 280 nm and 300 nm was much lower. The k value at 280 nm was close to that at 265 nm for feline calicivirus and MS2, suggesting that 280 nm UV-LED can be as good an option as 265 nm UV-LED to inactivate these viruses. Bacteria tended to show fluence-response curves with shoulder and tailing, while viruses followed log-linear profiles at all wavelengths tested. This study indicates the fluence-response profiles and the fluence required for a target inactivation of microorganisms, which would serve as reference data for future study and applications of UV-LEDs.

scholarly journals Using UVC Light-Emitting Diodes at Wavelengths of 266 to 279 Nanometers To Inactivate Foodborne Pathogens and Pasteurize Sliced Cheese

2015 ◽  
Vol 82 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Soo-Ji Kim ◽  
Do-Kyun Kim ◽  
Dong-Hyun Kang
Keyword(s):  
Foodborne Pathogens ◽  
Light Emitting Diodes ◽  
Uv Light ◽  
Content Type ◽  
Warm Up ◽  
Light Emitting ◽  
Uv Led ◽  
Serovar Typhimurium ◽  
Uv Lamps ◽  
Uv Leds

ABSTRACTUVC light is a widely used sterilization technology. However, UV lamps have several limitations, including low activity at refrigeration temperatures, a long warm-up time, and risk of mercury exposure. UV-type lamps only emit light at 254 nm, so as an alternative, UV light-emitting diodes (UV-LEDs) which can produce the desired wavelengths have been developed. In this study, we validated the inactivation efficacy of UV-LEDs by wavelength and compared the results to those of conventional UV lamps. Selective media inoculated withEscherichia coliO157:H7,Salmonella entericaserovar Typhimurium, andListeria monocytogeneswere irradiated using UV-LEDs at 266, 270, 275, and 279 nm in the UVC spectrum at 0.1, 0.2, 0.5, and 0.7 mJ/cm2, respectively. The radiation intensity of the UV-LEDs was about 4 μW/cm2, and UV lamps were covered with polypropylene films to adjust the light intensity similar to those of UV-LEDs. In addition, we applied UV-LED to sliced cheese at doses of 1, 2, and 3 mJ/cm2. Our results showed that inactivation rates after UV-LED treatment were significantly different (P< 0.05) from those of UV lamps at a similar intensity. On microbiological media, UV-LED treatments at 266 and 270 nm showed significantly different (P< 0.05) inactivation effects than other wavelength modules. For sliced cheeses, 4- to 5-log reductions occurred after treatment at 3 mJ/cm2for all three pathogens, with negligible generation of injured cells.

ACCURATE MEASUREMENT OF ULTRAVIOLET LIGHT-EMITTING DIODES

2021 ◽  
Author(s):  
C. Yuqin Zong ◽  
Cameron Miller
Keyword(s):  
Ultraviolet Light ◽  
Light Emitting Diodes ◽  
Luminous Flux ◽  
Junction Temperature ◽  
Integrating Sphere ◽  
Light Emitting ◽  
Type D ◽  
Uv Led ◽  
Uv Leds ◽  
Ultraviolet Light Emitting Diodes

We have developed a new calibration capability for 200 nm to 400 nm ultraviolet light-emitting diodes (UV LEDs) using a Type D gonio-spectroradiometer. The recently-introduced mean differential continuous pulse (M-DCP) method is used to overcome the measurement difficulty associated with the initial forward voltage, VF, anomaly of a UV LED, which makes it impossible to use VF to infer junction temperature, TJ, during pulsed operation. The new measurement facility was validated indirectly by comparing the measured total luminous flux of a white LED with that measured using the NIST’s 2.5 m absolute integrating sphere. The expanded calibration uncertainty for the total radiant flux is approximately 2 % to 3 % (k = 2) depending the wavelength of the UV LED.

scholarly journals Transcriptome and metabolome analyses revealed that narrowband 280 and 310 nm UV-B induce distinctive responses in Arabidopsis

2021 ◽  
Author(s):  
Tomohiro Tsurumoto ◽  
Yasuo Fujikawa ◽  
Daisaku Ohta ◽  
Atsushi Okazawa
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Uv Light ◽  
Current Model ◽  
Phenylpropanoid Pathway ◽  
Metabolic Responses ◽  
Uv Resistance ◽  
Light Emitting ◽  
Uv Led ◽  
Uv Leds ◽  
Led Irradiation

SUMMARYIn plants, the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) perceives UV-B and induces UV-B responses including synthesis of UV-B absorbing phenolic compounds such as anthocyanins. UVR8 absorbs a range of UV-B (260–335 nm). However, the responsiveness of plants to each UV-B wavelength has not been intensively studied so far. Here, we performed transcriptome and metabolome analyses of Arabidopsis using UV light emitting diodes (LEDs) with peak wavelengths of 280 and 310 nm to investigate the differences in the wavelength-specific UV-B responses. Irradiation with both UV-LEDs induced gene expression of the transcription factor ELONGATED HYPOCOTYL 5 (HY5), which has a central role in the UVR8 signaling pathway. However, the overall transcriptomic and metabolic responses to 280 and 310 nm UV-LED irradiation were different. Most of the known UV-B-responsive genes, such as salicylic acid, jasmonic acid, and defense-related genes, responded only to 280 nm UV-LED irradiation. Lipids, polyamines and organic acids were the metabolites most affected by 280 nm UV-LED irradiation, whereas the effect of 310 nm UV-LED irradiation on the metabolome was considerably less. Enzymatic genes involved in the phenylpropanoid pathway upstream in anthocyanin biosynthesis were up-regulated only by 280 nm UV-LED irradiation. On the other hand, no enzymatic genes downstream in anthocyanin biosynthesis were induced by the UV-LEDs, but rather, they were down-regulated by 310 nm UV-LED irradiation. These results revealed that the responsivenesses of Arabidopsis to 280 and 310 nm UV-B were significantly different, suggesting that UV-B signaling is mediated by more complex pathways than the current model.

scholarly journals Double-Sided Freeform Lens for Light Collimation of Light Emitting Diodes

2019 ◽  
Vol 9 (24) ◽  
pp. 5452
Author(s):  
Yong-Sin Syu ◽  
Chun-Ying Wu ◽  
Yung-Chun Lee
Keyword(s):  
Light Emitting Diodes ◽  
Uv Light ◽  
Design Parameters ◽  
Injection Molding Process ◽  
Light Sources ◽  
Molding Process ◽  
Light Emitting ◽  
Uv Led ◽  
Simulation Results ◽  
Freeform Lens

A double-sided freeform lens is proposed for collimating light emitted from light emitting diodes (LEDs). The surface profiles of the lens are mathematically characterized and precisely determined based on a point-source assumption and differential geometry theory. The proposed lens design method is straightforward, flexible, and effective. Moreover, the optical performance of the lens can be intuitively adjusted by tuning just a small number of design parameters. The simulation results showed that the proposed lens achieved an excellent collimating effect for a commercial ultraviolet (UV) LED. A prototype lens is fabricated in UV-grade poly(methyl methacrylate) material using a standard injection molding process. The light collimating effect of the lens/UV-LED assembly was measured experimentally and was shown to be in good agreement with the simulation results. The collimating angle at the half-energy level was equal to 1.88°. The performance of the UV-LED is thus comparable to that of conventional lithography UV light sources based on mercury arc lamps. Consequently, the proposed double freeform lens showed significant potential for photolithography applications within the industry.

Current Injection UV-Emission from InAlGaN Multi-Quantum-Well Light-Emitting Diodes

2000 ◽  
Vol 639 ◽  
Author(s):  
A. Kinoshita ◽  
H. Hirayama ◽  
M. Ainoya ◽  
J. S. Kim ◽  
A. Hirata ◽  
...  
Keyword(s):  
Quantum Well ◽  
Light Emitting Diodes ◽  
Uv Light ◽  
Current Injection ◽  
Light Emitting ◽  
Organic Vapor ◽  
Uv Emission ◽  
Metal Organic ◽  
Uv Leds ◽  
Multi Quantum Well

ABSTRACTInAlGaN quaternary material is very attractive for realizing ultraviolet (UV) emitting devices working at 300 – 350 nm wavelength range. We demonstrate current injection into 340 nm-band InAlGaN based UV light emitting diodes (LEDs), for the first time, fabricated by metal organic vapor phase epitaxy (MOVPE). We performed current injection into AlGaN/AlGaN multi quantum well (MQW), bulk InAlGaN quaternary and InAlGaN/InAlGaN MQW LEDs through Mg-doped AlGaN/GaN superlattice hole conductive layers. The injected current density was ranging 0 – 0.5 kA/cm2 under pulsed or CW operation. The intensity of both photoluminescence (PL) and electroluminescence for InAlGaN quaternary-based LED was much higher than that for AlGaN based LEDs at room temperature. From these results InAlGaN quaternary-based QWs are expected to realize high intensity UV LEDs and LDs.

Progress and Perspective of Near-Ultraviolet and Deep-Ultraviolet Light-Emitting Diode Packaging Technologies

2019 ◽  
Vol 141 (4) ◽  
Author(s):  
Yang Peng ◽  
Renli Liang ◽  
Yun Mou ◽  
Jiangnan Dai ◽  
Mingxiang Chen ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Light Emitting Diodes ◽  
Light Emitting ◽  
Near Ultraviolet ◽  
Uv Led ◽  
Duv Led ◽  
Deep Ultraviolet ◽  
Uv Leds ◽  
Ultraviolet Light Emitting Diodes ◽  
Light Efficiency

Abstract Ultraviolet light-emitting diodes (UV-LEDs) have drawn considerable attention in environment, life science, and industry fields, such as the applications of near UV-LEDs in resin curing, illumination, and identification, and deep UV-LEDs in disinfection, medical treatment, and biochemical inspection. However, due to the limitation of packaging technology, UV-LED devices exhibit low light efficiency and poor reliability compared with visible LEDs. The organic encapsulation materials are prone to UV aging, thermal degradation, and nonairtightness, which significantly reduce the performances of UV-LEDs. In order to solve this issue, UV-LED packaging technology has been proposed for UV-LED devices instead of conventional LED packaging. In this review, we investigated in detail the overview and challenges of near-ultraviolet light-emitting diodes (NUV-LED)/deep-ultraviolet light-emitting diodes (DUV-LED) packaging. For the packaging of UV-LEDs, all inorganic encapsulation materials, hermetic packaging structures with low-temperature bonding, reduced reflection losses, UV stable and transparent materials, and effective thermal management are key progresses to enhance the light efficiency and reliability of UV-LEDs. In addition, the summary and perspectives of NUV-LED/DUV-LED packaging were introduced and discussed.

scholarly journals Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses

2020 ◽  
Vol 8 (7) ◽  
pp. 1014
Author(s):  
Mizuki Kojima ◽  
Kazuaki Mawatari ◽  
Takahiro Emoto ◽  
Risa Nishisaka-Nonaka ◽  
Thi Kim Ngan Bui ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Influenza A ◽  
Light Emitting Diodes ◽  
Strongly Correlated ◽  
Influenza A Viruses ◽  
Light Emitting ◽  
Uv Led ◽  
Uv Lamp ◽  
Uv Leds ◽  
Ultraviolet Light Emitting Diodes

Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock. This study aimed to determine the ideal irradiation by ultraviolet-light emitting diodes (UV-LEDs) for IAV disinfection. We irradiated the IAV H1N1 subtype with 4.8 mJ/cm2 UV using eight UV-LEDs [peak wavelengths (WL) = 365, 310, 300, 290, 280, 270, and 260 nm)] or a mercury low pressure (LP)-UV lamp (Peak WL = 254 nm). Inactivation was evaluated by the infection ratio of Madin–Darby canine kidney (MDCK) cells or chicken embryonated eggs. Irradiation by the 260 nm UV-LED showed the highest inactivation among all treatments. Because the irradiation-induced inactivation effects strongly correlated with damage to viral RNA, we calculated the correlation coefficient (RAE) between the irradiant spectrum and absorption of viral RNA. The RAE scores strongly correlated with the inactivation by the UV-LEDs and LP-UV lamp. To increase the RAE score, we combined three different peak WL UV-LEDs (hybrid UV-LED). The hybrid UV-LED (RAE = 86.3) significantly inactivated both H1N1 and H6N2 subtypes to a greater extent than 260 nm (RAE = 68.6) or 270 nm (RAE = 42.2) UV-LEDs. The RAE score is an important factor for increasing the virucidal effects of UV-LED irradiation.

Performance of vertical type deep UV light-emitting diodes depending on the Ga-face n-contact hole density

2021 ◽  
Vol 118 (23) ◽  
pp. 231102
Author(s):  
Youn Joon Sung ◽  
Dong-Woo Kim ◽  
Geun Young Yeom ◽  
Kyu Sang Kim
Keyword(s):  
Light Emitting Diodes ◽  
Uv Light ◽  
Hole Density ◽  
Light Emitting ◽  
Deep Uv

New Results on Electroluminescence from Porous Silicon

1992 ◽  
Vol 283 ◽  
Author(s):  
Peter Steiner ◽  
Frank Kozlowski ◽  
Hermann Sandmaier ◽  
Walter Lang
Keyword(s):  
Porous Silicon ◽  
Quantum Efficiency ◽  
Light Emitting Diodes ◽  
Uv Light ◽  
Green Light ◽  
Light Emitting ◽  
Porous Region ◽  
First Results

ABSTRACTFirst results on light emitting diodes in porous silicon were reported in 1991. They showed a quantum efficiency of 10-7 to 10-5 and an orange spectrum. Over the last year some progress was achieved:- By applying UV-light during the etching blue and green light emitting diodes in porous silicon are fabricated.- When a p/n junction is realized within the porous region, a quantum efficiency of 10-4 is obtained.

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