Comparison between estrogenicities estimated from DNA recombinant yeast assay and from chemical analyses of endocrine disruptors during sewage treatment

2001 ◽  
Vol 43 (2) ◽  
pp. 125-132 ◽  
Author(s):  
H. Tanaka ◽  
Y. Yakou ◽  
A. Takahashi ◽  
T. Higashitani ◽  
K. Komori

This study discusses the estrogenicity and the extent of estrogenic effects, of sewage and treated sewage in public sewage treatment plants in Japan. The estrogenicity in this study was measured with a DNA recombinant yeast strain. Using this method, 43 chemicals that are suspected to have estrogen-like effects were measured and their estrogenicities were evaluated in terms of 17β-estradiol equivalents by comparison with the estrogenicity of 17β-estradiol. 17β-estradiol equivalents of influent and effluent sampled from 20 sewage treatment plants (STPs) were measured with this method. Because the concentrations of endocrine disruptors (EDs) in the STPs were monitored by the Ministry of Construction (MOC), the estrogenic effects estimated from the chemical data were obtained as a theoretical estrogenicity in terms of 17β-estradiol equivalent. The results suggest that STPs effectively reduce the estrogenicity and the theoretical estrogenicity during treatment, and that there were some differences between the estrogenicity assayed by the yeast and the theoretical estrogenicity in many STPs, particularly in influent sewage. Therefore, it is implied that unknown estrogen-like substances or antagonists might exist in influent sewage and treated sewage in STPs.

2008 ◽  
Vol 37 (2) ◽  
Author(s):  
Maciej Walczak

Changes of microbial indices of water quality in the Vistula and Brda rivers as a result of sewage treatment plant operationThis paper reports the results of studies of microbiological changes in the water quality of the Vistula and Brda rivers after the opening of sewage treatment plants in Bydgoszcz. The study involved determining the microbiological parameters of water quality. Based on the results obtained, it was found that the quality of the water in both rivers had improved decidedly after the opening of the plants, although an increased number of individual groups of microorganisms was found at the treated sewage outlet from one of the plants.


Author(s):  
Juliana Pinheiro Gomes ◽  
Giulia Oliveira Timo ◽  
Lícia Murito de Paula ◽  
Thales Viana Labourdette Costa ◽  
Paulo Roberto de Souza Rocha Júnior ◽  
...  

Emerging contaminants such as 17α-ethynylestradiol (EE2) can be discharged from sewage systems and contaminate water supplies. Paranoá Lake is a strategic water reservoir in Brasília (Brazil) that receives treated sewage from sewage treatment plants (STPs) and has recently become a water supply. This study monitored EE2 residues in different matrices from Paranoá Lake watershed using the ELISA method. This monitoring was performed together with the local environmental agency in two periods. EE2 was detected in all sewage samples showing that this residue is continually being discharged into the lake. However, EE2 was found in only one freshwater sample (0.07 ng L-1), which is below the predicted no-effect concentration considered as a risk for aquatic animals. EE2 was not detected in treated water. Nevertheless, the increased use of freshwater as a water supply signals the need for continuous EE2 monitoring in the lake.


1993 ◽  
Vol 27 (5-6) ◽  
pp. 357-367 ◽  
Author(s):  
J. Hultgren ◽  
L. G. Reinius

The requirements for the sewage treatment plants in Sweden will be more stringent in the future. The three plants in Stockholm, Henriksdal, Bromma and Loudden have to be extended to meet requirements for nitrogen removal and increased phosphorus removal. Since 1989 the plants have the same recipient for the treated sewage and to save money during the future extension the city would like to regard the three effluents as one common. The authorities have not yet decided to admit this but there is a good chance for it. The paper describes the strategy for extension and how the plants have been designed.


2012 ◽  
Vol 18 (2) ◽  
pp. 317-323 ◽  
Author(s):  
Raphael Bastão de Souza ◽  
Carmem Silvia Fontanetti

AbstractThe final disposal of residues generated at sewage treatment plants (STPs) has become a major problem for cities, due to the increase in the amount of treated sewage. One of the alternatives for the residue, labeled “sewage sludge,” is its reuse in agriculture and in degraded soil. However, not all pathogens and metals present in it are eliminated during treatment. Diplopods have been used as bioindicators in ecotoxicological tests as they are constantly in close contact with the soil. Owing to this fact, the purpose of this study was to expose specimens of the diplopod Rhinocricus padbergi to substrate containing sewage sludge collected at STPs to analyze morphological alterations in their parietal and perivisceral fat body, where substances are stored. The exposures were held for 7, 15, or 90 days at different concentrations of sewage sludge (control, 1%, 10%, and 50%). The parietal fat body showed no alterations in any of the three exposure periods or concentrations. Alterations in the perivisceral fat body were observed for all exposure periods. According to the results, we suggest that the sludge used has toxic agents responsible for changing the animal's perivisceral fat body.


2003 ◽  
Vol 47 (9) ◽  
pp. 71-76 ◽  
Author(s):  
A. Takahashi ◽  
T. Higashitani ◽  
Y. Yakou ◽  
M. Saitou ◽  
H. Tamamoto ◽  
...  

There are two major routes through which fish are exposed to endocrine disruptors (EDs); one route is through water that is a habitat; the other is through aquatic food such as algae and benthos. Few studies on the bioaccumulation of EDs in food have been conducted. Therefore, we evaluated the concentration in food of nonylphenol (NP), bisphenol A (BPA) and 17β-estradiol (E2), which were frequently detected in river water and in final discharge of Wastewater Treatment Plants (WWTPs) in Japan. We also evaluated the estrogenicity of samples using recombinant yeast. NP concentrations ranged 0.1-0.4 mg/L in the river water, while they ranged 8-130 mg/kg-wet in the periphytons and 8-140 μg/kg-wet in the benthos. BPA concentrations ranged 0.02-0.15 μg/L in the river water, while they ranged 2-8.8 μg/kg-wet in the periphytons and 0.3-12 μg/kg-wet in the benthos. E2 concentrations ranged 0.0001-0.0076 μg/L in the water, while they ranged 0.09-2.26 μg/kg-wet in the periphytons and <0.01-0.22 μg/kg-wet in the benthos. The estrogenicity ranged 0.0001-0.0464 μg-E2equivalent/L in the water, while it ranged 3.4-66.8 μg-E2equivalent/kg-wet in the periphytons and 7.4-5458 μg-E2equivalent/kg-wet in the benthos. Bioaccumulation factors of NP are estimated as 160-650 for the periphytons, and 63-990 for the benthos, respectively. Bioaccumulation factors of BPA are estimated as 18-650 for the periphytons, and 8-170 for the benthos, respectively. Bioaccumulation factors of E2 are estimated as 64-1,200 for the periphytons, and 100-160 for the benthos, respectively. The ratios of the periphytons and the benthos to the water in terms of the estrogenicity were larger than those in terms of the chemicals. In particularly, the ratio of the benthos to the water is about 106 in the maximum. The results suggest that food may be a more important route for fish exposed to EDs in water environment.


2008 ◽  
Vol 58 (11) ◽  
pp. 2187-2194 ◽  
Author(s):  
H. M. Coleman ◽  
S. J. Khan ◽  
G. Watkins ◽  
R. M. Stuetz

There are limited studies on the fate and levels of endocrine disrupting chemicals in sewage treatment plants in Australia. Research undertaken in Europe and North America has shown biologically significant levels of both oestrogenic and androgenic chemicals in sewage effluent. The aim of this work was to determine the oestrogenic and androgenic activities of raw and treated sewage from sewage treatment plants run by MidCoast Water, New South Wales, Australia. Oestrogenic and androgenic activities were measured using a yeast screen bioassay. Results showed that the raw effluent contained biologically significant levels of both oestrogenic (0.58–2.91 ng/l) and androgenic (216–480 ng/l) activities. Androgenic activity was significantly higher than oestrogenic activity, which was consistent with other Australian studies and was attributed to the higher levels of androgens in domestic waste from human excretion compared to oestrogens. Secondary treatment (using activated sludge) removed the majority of the oestrogenic and androgenic activity (up to 99%). Tertiary treatment by UV removed varying levels of oestrogenic (19–69%) and androgenic (5–55%) activities. A Membrane Bioreactor (MBR) at one of the STPs, which consists of an MBR followed by electrochlorination removed over 87% of the oestrogenic activity and over 98% of androgenic activity from raw sewage samples. However, levels which could be biologically significant still remained after secondary and tertiary treatment (>0.1 ng/l oestrogenic activity and >1 ng/l androgenic activity).


Author(s):  
Giuliano Gabrielli ◽  
Jorge L. da Paixão ◽  
Bruno Coraucci ◽  
Adriano L. Tonetti

<title>ABSTRACT</title><p>Effluents from secondary sewage treatment plants may contain amount of nutrients with the potential to cause eutrophication of water bodies. One of the ways to decrease this influx of nutrients would be the agricultural reuse. Therefore, the objective of this study was to evaluate the irrigation of roses of the Ambiance variety with the effluent from an anaerobic reactor and an intermittent sand filter (nitrified effluent). The trial lasted for 152 days. The dose of nitrogen applied via effluent was 91% of the crop requirement; of phosphorus, 3.5% (very low); and of potassium, 23%. There was no difference among treatments for the evaluated variables: diameter and length of stem, diameter and length of bud, and fresh weight of flower buds. The production of floral stems was higher in treatments with effluents without conventional fertilization. It is concluded that the irrigation with a treated effluent is an alternative for the fertilization of ambiance roses.</p>


2021 ◽  
Vol 2021 ◽  
Author(s):  
Karolina Wójcicka

This article presents a detailed analysis of the functioning of municipal sewage treatment plants. The presented findings are based on questionnaires from over seventy wastewater treatment facilities, covering from several hundred to several hundred thousand inhabitants. The required quality of treated sewage and the necessary efficiency level of the treatment plant were determined in the context of the content of the applicable regulations, and were then compared with the actual data obtained from sewage treatment plants. The findings provided the basis for formulating an evaluation of the efficiency of municipal sewage treatment plants and for further analyses of the possibility of the recovery of water from sewage and its reuse.


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