Measurement of microorganisms with PHA production capability in activated sludge and its implication in Activated Sludge Model No. 3

2002 ◽  
Vol 45 (6) ◽  
pp. 107-113 ◽  
Author(s):  
S. Hanada ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Activated Sludge ◽  
Environmental Conditions ◽  
Nile Blue ◽  
Activated Sludge Model ◽  
Nile Blue A ◽  
Production Capability ◽  
Heterotrophic Microorganisms ◽  
Storage Rate ◽  
Pha Production ◽  
Dual Staining

In Activated Sludge Model No.3 (ASM3), it is hypothesized that all heterotrophic microorganisms (XH) can store substrate. However, in reality, both microorganisms with and without substrate storage capability (XH/STO and XH/S, respectively) could exist. If the ratio of XH/STO in activated sludge is influenced by operational and environmental conditions, kSTO (storage rate constant of heterotrophic microorganisms which is defined in ASM3) may not be a universal parameter and can change from case to case. In this study, polyhydroxyalkanoate (PHA) is assumed as the principal storage product, and the ratio of microorganisms with PHA production capability (XH/PHA) in various activated sludges was estimated by the dual staining of Nile Blue A (NB) and DAPI. It was shown that the ratio of XH/PHA in sludge varied among different municipal and laboratory activated sludges.

Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A

2011 ◽  
Vol 64 (3) ◽  
pp. 747-753 ◽  
Author(s):  
M. Oshiki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Nile Blue ◽  
Maximum Level ◽  
Fluorescence Measurement ◽  
Batch Reactors ◽  
Floc Size ◽  
Nile Blue A ◽  
On Line ◽  
Rapid Quantification

The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated sludge samples collected from laboratory scale sequencing batch reactors and full-scale wastewater treatment plants were stained with Nile blue A and their fluorescence intensities were determined. There was a high correlation (R2 > 0.97) between the fluorescence intensities of Nile blue A and PHA concentrations in activated sludge determined by gas chromatography. The Nile blue A staining and fluorescence measurement method allows us to determine PHA concentrations in activated sludge within only five minutes and up to 96 samples can be measured at once by using microplate reader. On-line monitoring of PHA concentrations in activated sludge was achieved by using a fluorometer equipped with a flow cell and the time point at which PHA concentration in activated sludge reached the maximum level could be identified. In addition, we examined the influence of pH, floc size and co-existing chemicals in activated sludge suspension on the fluorescence intensities of Nile blue A.

Acetate uptake by PHA-accumulating and non-PHA-accumulating organisms in activated sludge from an aerobic sequencing batch reactor fed with acetate

2010 ◽  
Vol 62 (1) ◽  
pp. 8-14 ◽  
Author(s):  
M. Oshiki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Activated Sludge ◽  
Sequencing Batch Reactor ◽  
Nile Blue ◽  
Batch Reactor ◽  
Reducing Power ◽  
Dapi Staining ◽  
Microbial Cells ◽  
Nile Blue A ◽  
Acetate Uptake ◽  
Uptake Rates

The present study was conducted to evaluate the specific acetate uptake rates of microorganisms with and without polyhydroxyalkanoates (PHA) accumulation. Activated sludge was aerobically incubated with 75 mgC L−1 radiolabeled or non-labeled acetate, and acetate consumption and PHA accumulation were monitored. Microorganisms were quantified as follows: all microbial cells by DAPI staining, whole acetate utilizing organisms by microautoradiography, and PHA-accumulating organisms by staining with Nile blue A. The abundance of acetate-utilizing organisms without PHA accumulation was also calculated from the outcomes. The estimate of acetate utilized by PHAAOs included both the acetate converted to PHA and that used to supply reducing power and ATP. Acetate utilized by PHAAOs and non-PHAAOs were divided by their respective abundances to obtain their respective specific acetate uptake rates: PHAAOs ranged between 5.3 and 8.0 × 10−10 mgC cell−1 h−1, and non-PHAAOs ranged between 2.8 and 4.2 × 10−10 mgC cell−1 h−1.

scholarly journals Evidence for Dual Site Binding of Nile Blue A toward DNA: Spectroscopic, Thermodynamic, and Molecular Modeling Studies

ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2613-2625
Author(s):  
Mukti Mohammad ◽  
Harun Al Rasid Gazi ◽  
Kumud Pandav ◽  
Prateek Pandya ◽  
Md. Maidul Islam
Keyword(s):  
Molecular Modeling ◽  
Nile Blue ◽  
Nile Blue A ◽  
Modeling Studies ◽  
Molecular Modeling Studies ◽  
Dual Site ◽  
Site Binding

Optimizing the Step-Feed Ratio and Modeling the Effluents of Aerobic Tank with ASM1 in ECOSUNIDE Process

2012 ◽  
Vol 178-181 ◽  
pp. 526-530
Author(s):  
Ruo Gu Li ◽  
Yan Qiu Zhang
Keyword(s):  
Organic Matter ◽  
Activated Sludge ◽  
Ammonia Nitrogen ◽  
Feed Ratio ◽  
Optimum Ratio ◽  
Matlab Simulink ◽  
Activated Sludge Model ◽  
Model Based ◽  
Autotrophic Bacteria ◽  
Optimum Model

The step feed model based on the Activated Sludge Model No.1 (ASM1) and the optimum model of the ammonia nitrogen (SNH) removal in wastewater were established. Four aeration tanks under the different step feed ratios were simulated by Matlab Simulink. The results show that single-feeding is conducive to the removal of readily biodegradable substrate (SS) and the growth of heterotrophic organisms (XBH), and to lower the biodegradable substrate (XS) at the same time. The SS, XS, and SNH concentrations are 1.36, 5.98, and 3.02 mg/L respectively in effluent. However, the step-feeding is conducive to the SNH removal, and the autotrophic bacteria (XBA) growth. Under the step feed ratio (25/25/25/25%), the SS, XS, and SNH concentrations are 2.64, 10.79, and 2.61 mg/L respectively. Under the optimum ratio (28.7/23.6/20.4/27.2%), step-feeding could further facilitate the removal of SNH and hinder the removal of organic matter, their concentrations are 2.70, 10.98, and 2.47 mg/L respectively.

Application of activated sludge model for phosphorus recovery potential simulation

2021 ◽  
Vol 232 ◽  
pp. 199-207
Author(s):  
Michal Preisner ◽  
Marzena Smol ◽  
Elena Neverova-Dziopak ◽  
Zbigniew Kowalewski
Keyword(s):  
Activated Sludge ◽  
Phosphorus Recovery ◽  
Activated Sludge Model ◽  
Recovery Potential

Towards a Comprehensive Model of Activated Sludge Bulking and Foaming

1992 ◽  
Vol 25 (6) ◽  
pp. 215-230 ◽  
Author(s):  
David Jenkins
Keyword(s):  
Activated Sludge ◽  
Comprehensive Model ◽  
Activated Sludge Model ◽  
Sludge Bulking ◽  
Physiological Properties

The physiological properties of filamentous and floc-forming microorganisms found in activated sludge are reviewed. From this review four model microorganisms - two floc formers and two filamentous organisms - are proposed for use in an activated sludge model that predicts activated sludge “quality”.

Interpretation of Experimental Data with Regard to the Activated Sludge Model No.1 and Calibration of the Model for Municipal Wastewater Treatment Plants

1992 ◽  
Vol 25 (6) ◽  
pp. 167-183 ◽  
Author(s):  
H. Siegrist ◽  
M. Tschui
Keyword(s):  
Experimental Data ◽  
Growth Rate ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Municipal Wastewater Treatment ◽  
Activated Sludge Model

The wastewater of the municipal treatment plants Zürich-Werdhölzli (350000 population equivalents), Zürich-Glatt (110000), and Wattwil (20000) have been characterized with regard to the activated sludge model Nr.1 of the IAWPRC task group. Zürich-Glatt and Wattwil are partly nitrifying treatment plants and Zürich-Werdhölzli is fully nitrifying. The mixing characteristics of the aeration tanks at Werdhölzli and Glatt were determined with sodium bromide as a tracer. The experimental data were used to calibrate hydrolysis, heterotrophic growth and nitrification. Problems arising by calibrating hydrolysis of the paniculate material and by measuring oxygen consumption of heterotrophic and nitrifying microorganisms are discussed. For hydrolysis the experimental data indicate first-order kinetics. For nitrification a maximum growth rate of 0.40±0.07 d−1, corresponding to an observed growth rate of 0.26±0.04 d−1 was calculated at 10°C. The half velocity constant found for 12 and 20°C was 2 mg NH4-N/l. The calibrated model was verified with experimental dam of me Zürich-Werdhölzli treatment plant during ammonia shock load.

Activated Sludge Model No. 3

1999 ◽  
Vol 39 (1) ◽  
pp. 183-193 ◽  
Author(s):  
Willi Gujer ◽  
Mogens Henze ◽  
Takahashi Mino ◽  
Mark van Loosdrecht
Keyword(s):  
Activated Sludge ◽  
Activated Sludge Model
Sign in / Sign up

Export Citation Format

Share Document