Degradation of xenobiotic substances using sulfate-reducing bacteria in a UASB reactor

2004 ◽  
Vol 48 (11-12) ◽  
pp. 227-234 ◽  
Author(s):  
S. Tsuneda ◽  
T. Shiono ◽  
K. Nakamura ◽  
T. Dogan ◽  
A. Hirata
Keyword(s):  
Removal Efficiency ◽  
Yeast Extract ◽  
Sulfate Reducing Bacteria ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Ozone Treatment ◽  
Anaerobic Sludge ◽  
Equivalent Diameter ◽  
Sulfate Reducing ◽  
Reducing Bacteria

An upflow anaerobic sludge blanket (UASB) reactor was successfully applied to continuous degradation of ferric ethylene diamine tetraacetate (Fe-EDTA) as a typical xenobiotic substance contained in photo-processing wastewater. The sludge in the UASB reactor had an abundance of sulfate-reducing bacteria (SRB), which had been anaerobically cultivated in a sulfate-rich culture medium including Fe-EDTA and yeast-extract as the carbon sources. Since the prominent reductions of Fe-EDTA and sulfate ion were observed, the contribution of SRB to Fe-EDTA degradation in the UASB reactor was confirmed. The aggregated sludge in the UASB reactor became gradually large reaching steady state with an equivalent diameter of 60-90 μm after 124 days operation. An increase of the amount of yeast extract addition to feed solution improved the Fe-EDTA removal efficiency up to 90%. Moreover, the combination of ozone treatment with SRB treatment further improved removal efficiency of total organic carbon (TOC) in an actual photo-processing wastewater composed of fixing and developing wastes.

Enhanced Performance of Sulfate Reducing Bacteria (SRB) Granules Process by Using Diatomite as Functional Carrier Material

2017 ◽  
Vol 730 ◽  
pp. 206-211 ◽  
Author(s):  
Qi Yuan Gu ◽  
Xing Yu Liu ◽  
Zi Ning Wang
Keyword(s):  
Heavy Metals ◽  
Sulfate Reducing Bacteria ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Technical Solution ◽  
Carrier Material ◽  
Sulfate Reducing ◽  
Freely Suspended ◽  
Reducing Bacteria

Removal of heavy metals by sulfate reducing bacteria (SRB) granules in upflow anaerobic sludge blanket (UASB) reactor was investigated. Diatomite was adding to stimulate the formation of SRB sludge granule as a kind of functional carrier material. The diatomite was found to be the good bacteria carrier, due to its high porous structure, which promoted the granulation in the UASB. The results indicated that the Bio-diatomite SRB granule process can be operated at HRT of 15 h, and achieve high removal rates of total Fe, Cu2+, Zn2+ and Cd2+. Compared to the Bio-diatomite SRB granule, freely suspended SRB could be washed out of the reactor. The Bio-diatomite SRB granule process can be a technical solution for heavy metal containing wastewater treatment with an efficient reduction of heavy metals.

scholarly journals Microbial Diversity Dynamics in a Methanogenic-Sulfidogenic UASB Reactor

2021 ◽  
Vol 18 (3) ◽  
pp. 1305
Author(s):  
E. Fernández-Palacios ◽  
Xudong Zhou ◽  
Mabel Mora ◽  
David Gabriel
Keyword(s):  
Paper Industry ◽  
Chemical Species ◽  
Methanogenic Archaea ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Sulfate Reducing ◽  
Long Run

In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42−L−1 and a constant C/S ratio of 1.5 ± 0.3 g Cg−1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB.

scholarly journals Removal and Survival of Fecal Indicators in a Constructed Wetland after UASB Pre-Treatment

2021 ◽  
Vol 13 (16) ◽  
pp. 9302
Author(s):  
Fabio Conti ◽  
Elena Cristina Rada ◽  
Paolo Viotti ◽  
Massimo Raboni
Keyword(s):  
Removal Efficiency ◽  
Fecal Coliforms ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Experimental Plant ◽  
Fecal Indicators ◽  
Increased Risk ◽  
North Brazil ◽  
Pre Treatment

The experimentation plant, based on a sub-surface horizontal flow phytodepuration (SSHFP) unit with a pre-treatment by an upflow anaerobic sludge blanket (UASB) reactor, proved valuable in treating the sewage of a small rural community located in north Brazil. During a six-month trial, the plant achieved an average removal efficiency of 98.2% (1.74 log removal) for fecal coliforms (FC) and 96.0% (1.40 log removal) for Enterococci (EN), as well as 95.6% for BOD5, 91.0% for COD,00 and 95.4% for suspended solids (SS). The contribution of the UASB reactor to this overall performance was very significant as, alone, it achieved a yield of 62.7% for FC and 60% for EN, in addition to 65.2% for BOD5 and 65.0% for SS. EN was chosen, in addition to FC, because of its higher specificity and strong environmental persistence, leading to an increased risk to human health. In fact, the experimental results confirmed its lower removal efficiency compared to FC. The mechanical and biological mechanisms that led to such a removal efficiency of the two fecal indicators (FIs) are outlined in the article. The same mechanisms led to a good level of equivalence between the removal efficiency of the two FIs with the removal efficiency of SS and BOD5, for both the whole plant and the UASB reactor alone. The research demonstrated the close correlation between the concentrations of EN and FC for the plant effluent. This correlation can be explained by the following mathematical expression of the regression line Log EN = 0.2571 Log FC + 3.5301, with a coefficient of determination R2 = 0.912. This implies that the concentration of the more specific indicator EN could be calculated, with acceptable approximation, from the simple analysis of FC and vice versa. The experimental plant brought important health benefits to the local population. In particular, there were no significant odor emissions; moreover, the risk of fecal pathogenic diseases was drastically reduced; finally, there was no proliferation of insects and other disease vectors, due to the absence of stagnant or semi-stagnant water exposed to the atmosphere.

Arsenic removal and activity of a sulfate reducing bacteria-enriched anaerobic sludge using zero valent iron as electron donor

2020 ◽  
Vol 384 ◽  
pp. 121392 ◽  
Author(s):  
Olga Lidia Zacarías-Estrada ◽  
Lourdes Ballinas-Casarrubias ◽  
María Elena Montero-Cabrera ◽  
Rene Loredo-Portales ◽  
Erasmo Orrantia-Borunda ◽  
...  
Keyword(s):  
Electron Donor ◽  
Arsenic Removal ◽  
Sulfate Reducing Bacteria ◽  
Zero Valent Iron ◽  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals Black water treatment by an upflow anaerobic sludge blanket (UASB) reactor: a pilot study

2019 ◽  
Vol 80 (8) ◽  
pp. 1505-1511 ◽  
Author(s):  
Nathalie Dyane Miranda Slompo ◽  
Larissa Quartaroli ◽  
Grietje Zeeman ◽  
Gustavo Henrique Ribeiro da Silva ◽  
Luiz Antonio Daniel
Keyword(s):  
Removal Efficiency ◽  
Phase 1 ◽  
Oxygen Demand ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Organic Loading Rate ◽  
Anaerobic Sludge ◽  
Phase 3 ◽  
Black Water ◽  
Anaerobic Sludge Blanket

Abstract Decentralized sanitary wastewater treatment has become a viable and sustainable alternative, especially for developing countries and small communities. Besides, effluents may present variations in chemical oxygen demand (COD), biochemical oxygen demand (BOD) and total nitrogen values. This study describes the feasibility of using a pilot upflow anaerobic sludge blanket (UASB) reactor to treat wastewater with different organic loads (COD), using black water (BW) and sanitary wastewater, in addition to its potential for preserving nutrients for later recovery and/or reuse. The UASB reactor was operated continuously for 95 weeks, with a hydraulic retention time of 3 days. In Phase 1, the reactor treated simulated BW and achieved 77% CODtotal removal. In Phase 2, treating only sanitary wastewater, the CODtotal removal efficiency was 60%. Phase 3 treated simulated BW again, and CODtotal removal efficiency was somewhat higher than in Phase 1, reaching 81%. In Phase 3, the removal of pathogens was also evaluated: the efficiency was 1.96 log for Escherichia coli and 2.13 log for total coliforms. The UASB reactor was able to withstand large variations in the organic loading rate (0.09–1.49 kg COD m−3 d−1), in continuous operation mode, maintaining a stable organic matter removal.

Thermophilic Anaerobic Treatment of Sulfate-Rich Pulp and Paper Integrate Process Water

1991 ◽  
Vol 24 (3-4) ◽  
pp. 149-160 ◽  
Author(s):  
J. Rintala ◽  
J. L. Sanz Martin ◽  
G. Lettinga
Keyword(s):  
Sulfate Reduction ◽  
Removal Efficiency ◽  
Anaerobic Treatment ◽  
Cod Removal ◽  
Process Water ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Cod Removal Efficiency ◽  
Uasb Reactors

Anaerobic treatment of sulfate - rich (COD/SO4 ratio 1.4-2.1) clarified Whitewater from a thermomechanical pulping (TMP) process was studied in three laboratory-scale upflow anaerobic sludge blanket (UASB) reactors at 55°C and in batch digesters at 55° and 65°C. Different seed materials were used in the UASB reactors. The highest COD removal efficiency (effluent sulfide stripped) achieved was approximately 65 % in the UASB reactors. About 55 % COD removal efficiency was obtained at a loading rate of about 41 kgCODm−3d−1 in the UASB reactor seeded with thermophilic sludge cultivated with volatile fatty acids (VFAs). The total sulfide present in the liquid phase after anaerobic treatment accounted for approximately 65-78 % and 15-61 % of the removed COD in the batch digesters and the UASB reactors, respectively. Sulfate reduction was almost complete in the batch digesters, whereas about 24-64 % of sulfate was reduced in the UASB reactors. Acetate utilization for sulfate reduction was apparent in the batch digesters, whereas that could not be demonstrated in the UASB reactors. Sulfate reduction in the UASB reactors was obviously substrate limited. In conclusion, thermophilic anaerobic treatment is an alternative for the treatment of warm sulfate rich TMP process water.

Competition for H2 between sulfate reducers, methanogens and homoacetogens in a gas-lift reactor

2002 ◽  
Vol 45 (10) ◽  
pp. 75-80 ◽  
Author(s):  
J. Weijma ◽  
F. Gubbels ◽  
L.W. Hulshoff Pol ◽  
A.J.M. Stams ◽  
P. Lens ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Sulfate Reduction ◽  
Growth Kinetic ◽  
Settling Velocity ◽  
Sulfate Reducing Bacteria ◽  
Anaerobic Sludge ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Gas Lift

Reported values for growth kinetic parameters show an order in competitivity of heterotrophic sulfate reducing bacteria>methanogens>homoacetogens for the substrate hydrogen. This order suggests that methanogens can succesfully compete with consortia of heterotrophic SRB and homoacetogens when H2/CO2 is present as sole substrate. However, we found in experiments using gas-lift reactors inoculated with anaerobic sludge and fed with H2/CO2 and sulfate, that heterotrophic sulfate reduction rapidly and completely outcompeted methanogenesis, whereas a low amount of acetate was formed. Thus, in disagreement with the above competitivity order, hydrogen is more readily consumed by homoacetogenesis than by methanogenesis, indicating that the competition is not kinetically determined. The superior settling velocity of sulfidogenic-acetogenic sludge compared to that of methanogenic sludge suggests that the former sludge is better retained, which can explain the predominance of sulfate reduction/homoacetogenesis over methanogenesis.

Desulfovibrio mexicanus sp. nov., a Sulfate-reducing Bacterium Isolated from an Upflow Anaerobic Sludge Blanket (UASB) Reactor Treating Cheese Wastewaters

Anaerobe ◽  
2000 ◽  
Vol 6 (5) ◽  
pp. 305-312 ◽  
Author(s):  
Guadalupe Hernandez-Eugenio ◽  
Marie-Laure Fardeau ◽  
Bharat K.C. Patel ◽  
Hervé Macarie ◽  
Jean-Louis Garcia ◽  
...  
Keyword(s):  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Anaerobic Sludge Blanket
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