Competition for H2 between sulfate reducers, methanogens and homoacetogens in a gas-lift reactor

2002 ◽  
Vol 45 (10) ◽  
pp. 75-80 ◽  
Author(s):  
J. Weijma ◽  
F. Gubbels ◽  
L.W. Hulshoff Pol ◽  
A.J.M. Stams ◽  
P. Lens ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Sulfate Reduction ◽  
Growth Kinetic ◽  
Settling Velocity ◽  
Sulfate Reducing Bacteria ◽  
Anaerobic Sludge ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Gas Lift

Reported values for growth kinetic parameters show an order in competitivity of heterotrophic sulfate reducing bacteria>methanogens>homoacetogens for the substrate hydrogen. This order suggests that methanogens can succesfully compete with consortia of heterotrophic SRB and homoacetogens when H2/CO2 is present as sole substrate. However, we found in experiments using gas-lift reactors inoculated with anaerobic sludge and fed with H2/CO2 and sulfate, that heterotrophic sulfate reduction rapidly and completely outcompeted methanogenesis, whereas a low amount of acetate was formed. Thus, in disagreement with the above competitivity order, hydrogen is more readily consumed by homoacetogenesis than by methanogenesis, indicating that the competition is not kinetically determined. The superior settling velocity of sulfidogenic-acetogenic sludge compared to that of methanogenic sludge suggests that the former sludge is better retained, which can explain the predominance of sulfate reduction/homoacetogenesis over methanogenesis.

scholarly journals Two Marine Desulfotomaculum spp. of Different Origin are Capable of Utilizing Acetone and Higher Ketones

2021 ◽  
Author(s):  
Jasmin Frey ◽  
Sophie Kaßner ◽  
Bernhard Schink
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Thiamine Diphosphate ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria ◽  
Different Origin

AbstractDegradation of acetone and higher ketones has been described in detail for aerobic and nitrate-reducing bacteria. Among sulfate-reducing bacteria, degradation of acetone and other ketones is still an uncommon ability and has not been understood completely yet. In the present work, we show that Desulfotomaculum arcticum and Desulfotomaculum geothermicum are able to degrade acetone and butanone. Total proteomics of cell-free extracts of both organisms indicated an involvement of a thiamine diphosphate-dependent enzyme, a B12-dependent mutase, and a specific dehydrogenase during acetone degradation. Similar enzymes were recently described to be involved in acetone degradation by Desulfococcus biacutus. As there are so far only two described sulfate reducers able to degrade acetone, D. arcticum and D. geothermicum represent two further species with this capacity. All these bacteria appear to degrade acetone via the same set of enzymes and therefore via the same pathway.

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

1998 ◽  
Vol 37 (4-5) ◽  
pp. 599-603 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja. Kofi. Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Sulfate Reduction Rates ◽  
Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

Degradation of xenobiotic substances using sulfate-reducing bacteria in a UASB reactor

2004 ◽  
Vol 48 (11-12) ◽  
pp. 227-234 ◽  
Author(s):  
S. Tsuneda ◽  
T. Shiono ◽  
K. Nakamura ◽  
T. Dogan ◽  
A. Hirata
Keyword(s):  
Removal Efficiency ◽  
Yeast Extract ◽  
Sulfate Reducing Bacteria ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Ozone Treatment ◽  
Anaerobic Sludge ◽  
Equivalent Diameter ◽  
Sulfate Reducing ◽  
Reducing Bacteria

An upflow anaerobic sludge blanket (UASB) reactor was successfully applied to continuous degradation of ferric ethylene diamine tetraacetate (Fe-EDTA) as a typical xenobiotic substance contained in photo-processing wastewater. The sludge in the UASB reactor had an abundance of sulfate-reducing bacteria (SRB), which had been anaerobically cultivated in a sulfate-rich culture medium including Fe-EDTA and yeast-extract as the carbon sources. Since the prominent reductions of Fe-EDTA and sulfate ion were observed, the contribution of SRB to Fe-EDTA degradation in the UASB reactor was confirmed. The aggregated sludge in the UASB reactor became gradually large reaching steady state with an equivalent diameter of 60-90 μm after 124 days operation. An increase of the amount of yeast extract addition to feed solution improved the Fe-EDTA removal efficiency up to 90%. Moreover, the combination of ozone treatment with SRB treatment further improved removal efficiency of total organic carbon (TOC) in an actual photo-processing wastewater composed of fixing and developing wastes.

scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

scholarly journals Enhanced sulfate reduction with acidogenic sulfate-reducing bacteria

2008 ◽  
Vol 154 (1-3) ◽  
pp. 1060-1065 ◽  
Author(s):  
Aijie Wang ◽  
Nanqi Ren ◽  
Xu Wang ◽  
Duujong Lee
Keyword(s):  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM BIOFILM SULFATE REDUCTION AND CU PRECIPITATION BIOREACTOR (BATCH CULTURE).

2018 ◽  
Vol 2 (2) ◽  
pp. 1
Author(s):  
Tyas Nyonita Punjungsari
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Sulfate Reduction ◽  
Batch Culture ◽  
Microbial Community Structure ◽  
Sulfate Reducing Bacteria ◽  
Zeolite Surface ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The biofilm is a microbial community structure formed on the zeolite surface in a sulfate reduction bioreactor and Cu deposition using a SRB consortium . The biofilm soluble microbial solvent is expected to have the capability in sulfate reduction and Cu deposition. Characterization of isolates is required for the optimization of pure culture . The aim of this study is to isolate and characterize the biofilm sulfate reducing bacteria in the sulfate reduction bioreactor and the precipitation of Cu in Batch Culture by a consortium of Sulfate Reducing Bacteria. The method used in this study cultivation was done by using postgate B medium, isolation was done by diluting biofilm on NaCl salt, bacteria grown on NB and postgate B media, characterization done by morphology and biochemistry. There were 3 isolates of B1 (Metylobacterium ), B3 ( Desulfucoccus ), and B2 ( Desulfobacter ). B3 ( Desulfococcus) has the best ability to reduce sulfate and Cu precipitation.Keywords : Sulfur Reducing Bacteria (SRB), Biofilm, Sulfate, Cu. Received: 26 August, 2017; Accepter: 10 September, 2017 

Arsenic removal and activity of a sulfate reducing bacteria-enriched anaerobic sludge using zero valent iron as electron donor

2020 ◽  
Vol 384 ◽  
pp. 121392 ◽  
Author(s):  
Olga Lidia Zacarías-Estrada ◽  
Lourdes Ballinas-Casarrubias ◽  
María Elena Montero-Cabrera ◽  
Rene Loredo-Portales ◽  
Erasmo Orrantia-Borunda ◽  
...  
Keyword(s):  
Electron Donor ◽  
Arsenic Removal ◽  
Sulfate Reducing Bacteria ◽  
Zero Valent Iron ◽  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Reducing Bacteria
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