Post-treatment of a slaughterhouse wastewater: stability of the microbial community of a sequencing batch reactor operated under oxygen limited conditions

2006 ◽  
Vol 54 (2) ◽  
pp. 215-221 ◽  
Author(s):  
A. Cabezas ◽  
P. Draper ◽  
L. Muxí ◽  
C. Etchebehere
Keyword(s):  
Microbial Community ◽  
Organic Nitrogen ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Anaerobic Treatment ◽  
Post Treatment ◽  
Ammonia Oxidizing Bacteria ◽  
Slaughterhouse Wastewater ◽  
Nitrite Oxidizing Bacteria ◽  
The Stability

Slaughterhouse wastewater is a complex effluent with an important content of organic nitrogen. After an anaerobic treatment where most of the organic matter is removed, the nitrogen, remains as ammonium and post-treatment of the effluent is necessary. Sequencing batch reactor (SBR) technology has been developed to completely remove nitrogen in one single reactor combining aerobic and anoxic stages. Under oxygen limited conditions only nitrite is produced with concomitant energy saving. The stability and diversity of the microbial community from a nitrifying denitrifying SBR operated under oxygen limited conditions were studied using molecular and respirometric methods. The AOB (ammonia oxidizing bacteria) community was relatively stable Nitrosomonas being the dominant genera although Nitrosospira and Nitrosococcus were detected in low proportions. Nitrite oxidizing bacteria were out competed during the operation under oxygen-limited conditions. After an increase of the DO in the reactor Nitrobacter spp were detected suggesting that they remained in the system. Changes in the AOB and denitrifying communities were observed after the DO increase. Sedimentation problems were detected during operation, this could be related to the predominance of Thauera spp detected by FISH and T-RFLP.

Sequencing batch reactor as a post-treatment of anaerobically treated dairy effluent

2006 ◽  
Vol 54 (2) ◽  
pp. 199-206 ◽  
Author(s):  
A. Benítez ◽  
A. Ferrari ◽  
S. Gutierrez ◽  
R. Canetti ◽  
A. Cabezas ◽  
...  
Keyword(s):  
Batch Reactor ◽  
Anaerobic Treatment ◽  
Post Treatment ◽  
Operating Conditions ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Operational Parameters ◽  
Reactor Operation ◽  
Set Up ◽  
The Stability

Wastewater from dairy industries, characterized by its high COD content and relative high COD/TKN ratio, requires post-treatment after anaerobic treatment to complete the removal of organic matter and nutrients. Due to its simplicity, robustness and low maintenance costs, sequencing batch reactors (SBR) result in an attractive system, especially in case of small dairy industries in order to comply with the emission standards. The goal of this work was to determine the operational parameters, optimize the performance, and study the stability of the microbial population of a SBR system for the post-treatment of an anaerobic pond effluent. High and stable removal of COD and TKN was achieved in the reactor, which can easily be set up in dairy industries. An active nitrifying population was selected during reactor operation and maintained relatively stable, while the heterotrophic (total and denitrifying) communities were more unstable and susceptible to changes in the operating conditions.

Inhibitory effect of erythromycin, tetracycline and sulfamethoxazole antibiotics on anaerobic treatment of a pharmaceutical wastewater

2015 ◽  
Vol 71 (11) ◽  
pp. 1620-1628 ◽  
Author(s):  
Sevcan Aydın ◽  
Bahar Ince ◽  
Orhan Ince
Keyword(s):  
Microbial Community ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Anaerobic Treatment ◽  
Inhibitory Effect ◽  
Organic Content ◽  
Pharmaceutical Wastewater ◽  
Research Support ◽  
Anaerobic Sequencing Batch Reactor ◽  
High Organic Content

Pharmaceuticals enter ecosystems, which causes changes to microbial community structure and development of resistant genes. Anaerobic treatments can be an alternative application for treatment of pharmaceutical wastewaters, which has high organic content. This study aims to develop an understanding of the effects of sulfamethoxazole–erythromycin–tetracycline (ETS), sulfamethoxazole–tetracycline (ST), erythromycin–sulfamethoxazole (ES) and erythromycin–tetracycline (ET) combinations on the anaerobic treatment of pharmaceutical industry wastewater. The results of this investigation revealed that bacteria have a competitive advantage over archaea under all antibiotic combinations. The ET reactor showed a better performance compared to other reactors; this could be due to antagonistic effects of sulfamethoxazole. Acute inhibition in the microbial community was also strongly affected by antibiotics concentrations. This indicated that the composition of the microbial community changed in association with anaerobic sequencing batch reactor performances. The results of this research support the idea that an acute test could be used to control and improve the anaerobic treatment system.

Settleability and kinetics of a nitrifying sludge in a sequencing batch reactor

2004 ◽  
Vol 50 (11) ◽  
pp. 943-949 ◽  
Author(s):  
A -C Texier ◽  
J Gomez
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Operation Time ◽  
Volume Index ◽  
Specific Rate ◽  
Ammonia Oxidizing Bacteria ◽  
Microbial Protein ◽  
N Accumulation ◽  
Reactor Kinetics ◽  
Nitrite Oxidizing Bacteria

A physiological study of a nitrifying sludge was carried out in a sequencing batch reactor (SBR). Pseudo steady-state nitrification conditions were obtained with an ammonium removal efficiency of 99% ± 1% and 98% ± 2% conversion of NH4+-N to NO3–-N. The rate of biomass production was negligible (1.3 ± 0.1 mg microbial protein-N·L–1·d–1). The sludge presented good settling properties with sludge volume index values lower than 20 mL·g–1 and an exopolymeric protein/carbohydrate ratio of 0.53 ± 0.34. Kinetic results indicated that the nitrifying behavior of the sludge changed with the number of cycles. After 22 cycles, a decrease in the specific rate of NO3–-N production coupled with an increase in the NO2–-N accumulation were observed. These results showed that the activity of the nitrite oxidizing bacteria decreased at a longer operation time. Ammonia oxidizing bacteria were found to exhibit the best stability. After 4 months of operation, the specific rates of NH4+-N consumption and NO3–-N production were 1.72 NH4+-N per microbial protein-N per hour (g·g–1·h–1) and 0.54 NO3–-N per microbial protein-N per hour (g·g–1·h–1), respectively.Key words: nitrification, sequencing batch reactor, kinetics, settleability, exopolymeric substances.

The kinetics for ammonium and nitrite oxidation under the effect of hydroxylamine

2015 ◽  
Vol 73 (5) ◽  
pp. 1067-1073 ◽  
Author(s):  
Xinyu Wan ◽  
Pengying Xiao ◽  
Daijun Zhang ◽  
Peili Lu ◽  
Zongbao Yao ◽  
...  
Keyword(s):  
Sequencing Batch Reactor ◽  
Ammonia Oxidation ◽  
Oxygen Uptake Rate ◽  
Batch Reactor ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrite Oxidation ◽  
Kinetics Parameters ◽  
Nitrification Process ◽  
Nitrite Oxidizing Bacteria ◽  
No2 Oxidation

The kinetics for ammonium (NH4+) oxidation and nitrite (NO2−) oxidation under the effect of hydroxylamine (NH2OH) were studied by respirometry using the nitrifying sludge from a laboratory-scale sequencing batch reactor. Modified models were used to estimate kinetics parameters of ammonia and nitrite oxidation under the effect of hydroxylamine. An inhibition effect of hydroxylamine on the ammonia oxidation was observed under different hydroxylamine concentration levels. The self-inhibition coefficient of hydroxylamine oxidation and noncompetitive inhibition coefficient of hydroxylamine for nitrite oxidation was estimated by simulating exogenous oxygen-uptake rate profiles, respectively. The inhibitive effect of NH2OH on nitrite-oxidizing bacteria was stronger than on ammonia-oxidizing bacteria. This work could provide fundamental data for the kinetic investigation of the nitrification process.

Microbial community structure in aerobic and fluffy granules formed in a sequencing batch reactor supplied with 4-chlorophenol at different settling times

2018 ◽  
Vol 342 ◽  
pp. 606-616 ◽  
Author(s):  
Selene Gómez-Acata ◽  
Miguel Vital-Jácome ◽  
Mariana Vivian Pérez-Sandoval ◽  
Yendi E. Navarro-Noya ◽  
Frederic Thalasso ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Sequencing Batch Reactor ◽  
Batch Reactor

scholarly journals Partial nitritation treating nitrogen in old landfill leachate

2016 ◽  
Vol 19 (4) ◽  
pp. 39-49
Author(s):  
Nhat The Phan ◽  
Van Thi Thanh Truong ◽  
Son Thanh Le ◽  
Biec Nhu Ha ◽  
Dan Phuoc Nguyen
Keyword(s):  
Landfill Leachate ◽  
Retention Time ◽  
Sequencing Batch Reactor ◽  
Hydraulic Retention Time ◽  
Batch Reactor ◽  
Partial Nitritation ◽  
Time Operation ◽  
Nitrite Oxidizing Bacteria ◽  
Long Time ◽  
H 30

In this study, a lab-scale Partial Nitritation Sequencing Batch Reactor (PNSBR) was implemented for treating high-ammonium old landfill leachate to yield an appropriate NO2—N/ NH4+-N ratio from 1/1 to 1.32/1 mixture as a pretreatment for subsequent Anammox. The objective of this study was to determine the optimal hydraulic retention time (HRT) at different influent ammonia concentrations for 210 days. The experimental results showed that with the influent ammonia concentrations of 500, 1000, 1500 and 2000 mg/L, HRT is 12 h, 21 h, 30 h and 48 h, respectively. The range of free ammonia (FA) concentration from 17 to 44 mg/L completely inhibited nitrite oxidizing bacteria (NOB) for long time operation. The COD removal efficiency was very low (6±2) %.

Sign in / Sign up

Export Citation Format

Share Document