scholarly journals Simultaneous nitrification and phosphate removal by bioaugmented aerobic granules treating a fluoroorganic compound

2021 ◽  
Author(s):  
Anouk F. Duque ◽  
Vânia S. Bessa ◽  
Udo van Dongen ◽  
Merle K. de Kreuk ◽  
Raquel B. R. Mesquita ◽  
...  
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Amoa Gene ◽  
Granular Sludge ◽  
Phosphate Removal ◽  
Aerobic Granular Sludge ◽  
Ammonia Oxidizing Bacteria ◽  
Aerobic Granules ◽  
N Removal ◽  
Nitrite Oxidizing Bacteria ◽  
Gradient Gel Electrophoresis

Abstract The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated. Phosphate (P) and ammonium (N) removal efficiencies were high (>93%) and, after bioaugmentation with 2-FP degrading strain FP1, 2-FP was completely degraded. Neither N nor P removal were affected by 50 mg L−1 of 2-FP in the feed stream. Changes in the aerobic granule bacterial communities were followed. Numerical analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed low diversity for the amoA gene with an even distribution of species. PAOs, including denitrifying PAO (dPAO), and AOB were present in the 2-FP degrading granules, although dPAO population decreased throughout the 444 days reactor operation. The results demonstrated that the aerobic granules bioaugmented with FP1 strain successfully removed N, P and 2-FP simultaneously.

Elimination of NH3 and odor from composting by biotrickling filter and preliminary exploration on molecular biology

2011 ◽  
Vol 63 (4) ◽  
pp. 747-753 ◽  
Author(s):  
Niantao Xue ◽  
Qunhui Wang ◽  
Chuanfu Wu ◽  
Peng Zhao ◽  
Weimin Xie
Keyword(s):  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Biotrickling Filter ◽  
Ammonia Oxidizing Bacteria ◽  
Exhaust Gas ◽  
Chain Reaction ◽  
Volatile Organic ◽  
Nitrite Oxidizing Bacteria ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain

A biotrickling filter was focused on treatment of odorous NH3 gas, along with volatile organic compounds (VOCs) and other odorous gases during cattle manure composting. The biotrickling filter could treat NH3, the main composition, with removal efficiencies of close to 100% because NH4+ was bio-oxidized in the biofilm to NO3− resulting final NO3− concentrations of about 1000 mg/L. Results of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) revealed that ammonia-oxidizing bacteria, Nitrosospira sp, and nitrite-oxidizing bacteria, Nitrococcus mobilis, coexisted in the biofilm. There were 23 kinds of VOCs detected in the exhaust gas. A significant observation was that the biofilm itself of the biotrickling biofilter released VOCs. The odor concentrations of inlet and outlet gases were 1425 and 110, respectively, with removal efficiency of 92.2%, suggesting that the biotrickling filter was effective for treatment of odorous gases during composting.

scholarly journals Effect of Elevated Salt Concentrations on the Aerobic Granular Sludge Process: Linking Microbial Activity with Microbial Community Structure

2011 ◽  
Vol 77 (22) ◽  
pp. 7942-7953 ◽  
Author(s):  
J. P. Bassin ◽  
M. Pronk ◽  
G. Muyzer ◽  
R. Kleerebezem ◽  
M. Dezotti ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Granular Sludge ◽  
Phosphate Removal ◽  
Aerobic Granular Sludge ◽  
Nitrogen And Phosphorus ◽  
Content Type

ABSTRACTThe long- and short-term effects of salt on biological nitrogen and phosphorus removal processes were studied in an aerobic granular sludge reactor. The microbial community structure was investigated by PCR-denaturing gradient gel electrophoresis (DGGE) on 16S rRNA andamoAgenes. PCR products obtained from genomic DNA and from rRNA after reverse transcription were compared to determine the presence of bacteria as well as the metabolically active fraction of bacteria. Fluorescencein situhybridization (FISH) was used to validate the PCR-based results and to quantify the dominant bacterial populations. The results demonstrated that ammonium removal efficiency was not affected by salt concentrations up to 33 g/liter NaCl. Conversely, a high accumulation of nitrite was observed above 22 g/liter NaCl, which coincided with the disappearance ofNitrospirasp. Phosphorus removal was severely affected by gradual salt increase. No P release or uptake was observed at steady-state operation at 33 g/liter NaCl, exactly when the polyphosphate-accumulating organisms (PAOs), “CandidatusAccumulibacter phosphatis” bacteria, were no longer detected by PCR-DGGE or FISH. Batch experiments confirmed that P removal still could occur at 30 g/liter NaCl, but the long exposure of the biomass to this salinity level was detrimental for PAOs, which were outcompeted by glycogen-accumulating organisms (GAOs) in the bioreactor. GAOs became the dominant microorganisms at increasing salt concentrations, especially at 33 g/liter NaCl. In the comparative analysis of the diversity (DNA-derived pattern) and the activity (cDNA-derived pattern) of the microbial population, the highly metabolically active microorganisms were observed to be those related to ammonia (Nitrosomonassp.) and phosphate removal (“CandidatusAccumulibacter”).

Community analysis of nitrifying bacteria in an advanced and compact Gappei-Johkasou by FISH and PCR-DGGE

2002 ◽  
Vol 46 (11-12) ◽  
pp. 105-111 ◽  
Author(s):  
Y. Ebie ◽  
M. Matsumura ◽  
N. Noda ◽  
S. Tsuneda ◽  
A. Hirata ◽  
...  
Keyword(s):  
Community Structure ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Quantitative Estimation ◽  
Community Analysis ◽  
Nitrifying Bacteria ◽  
Ammonia Oxidizing Bacteria ◽  
Load Fluctuation ◽  
New Information ◽  
Nitrite Oxidizing Bacteria ◽  
Gradient Gel Electrophoresis

Fluorescent in situ hybridization (FISH) method with 16S rRNA-targeted oligonucleotide probes was used for quantitative estimation of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in a Johkasou. Although the occupation ratios of AOB and NOB increased as nitrification progressed, about one month later, the occupation ratios decreased, despite showing good nitrification ability. Furthermore, even when urea was added to the feeding wastewater to raise the amount of T-N, the occupation ratios of both nitrifying bacteria remained constant. For further investigation, denaturing gradient gel electrophoresis (DGGE) was used to study the community structure of AOB in the Johkasou. As a result, DGGE band patterns and following sequence analysis revealed that the community structure of AOB was complicated and changed during this experiment. It was suggested that even if the occupation ratio of AOB to eubacteria was constant, the majorities of AOB were changed through temperature and load fluctuation. The combination of FISH and PCR-DGGE provides new information that was not available by conventional cultivationbased methods.

Microbial community structure of activated sludge during aerobic granulation in an annular gap bioreactor

2006 ◽  
Vol 54 (1) ◽  
pp. 139-146 ◽  
Author(s):  
J.C. Williams ◽  
F.L. de los Reyes
Keyword(s):  
Loading Rate ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Annular Gap ◽  
Gradient Gel Electrophoresis ◽  
And Behavior ◽  
Cultivation Experiment

A novel annular gap reactor was designed to create a controlled shear environment in which aerobic granular sludge could be developed. The bacterial and eukaryal community structures during two aerobic granular sludge experiments were tracked using denaturing gradient gel electrophoresis (DGGE). The first granule cultivation experiment, using an organic loading rate of 1.6 kg/m3d COD, resulted in biomass that was dominated by filamentous bacteria and Zoogloea ramigera colonies. A second experiment with a higher organic loading rate of 6 kg/m3d COD developed a granule-like morphology but was ultimately dominated by filamentous fungi. Species identification via DGGE band purification and DNA sequencing closely matched the observed sludge morphology and behavior.

Bioaugmentation of Aerobic Granular Sludge with the Addition of a Bioflocculant-Producing Consortium

2013 ◽  
Vol 726-731 ◽  
pp. 2530-2535 ◽  
Author(s):  
Zhi Wei Song ◽  
Ting Ting Ning ◽  
Yu Ping Chen ◽  
Xiao Xia Cheng ◽  
Nan Qi Ren
Keyword(s):  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Bacterial Species ◽  
Granular Sludge ◽  
Extracellular Polysaccharide ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Aerobic Granular Sludge ◽  
Gradient Gel Electrophoresis ◽  
The Stability

A consortium of bioflocculant-producing bacteria (BPA), mainly consisting of previously enriched Devosia hwasunensis and Tetrasphaera elongata, was inoculated into a sequencing batch airlift reactor during aerobic granular sludge cultivation to determine the effects of BPA on the formation of aerobic granular sludges. The results indicate that granulation time was substantially shortened from 56 to 28 days with the addition of BPA. Microbial community analysis of granular sludge based on denaturing gradient gel electrophoresis revealed diversity. Dominant populations belonged to Actinobacteria and α-and γ-proteobacteria. The added bacterial species, D. hwasunensis and T. elongata, which have been proposed to secrete extracellular polysaccharide mucus, played an important role in particle formation and in the maintenance of the stability and physicochemical properties of the granular sludge.

Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge

2005 ◽  
Vol 90 (6) ◽  
pp. 761-769 ◽  
Author(s):  
M.K. de Kreuk ◽  
J.J. Heijnen ◽  
M.C.M. van Loosdrecht
Keyword(s):  
Granular Sludge ◽  
Phosphate Removal ◽  
Aerobic Granular Sludge ◽  
Nitrogen And Phosphate Removal

A comparitive study of ammonia-oxidizing bacteria in lab-scale industrial wastewater treatment reactors

2003 ◽  
Vol 48 (3) ◽  
pp. 17-24 ◽  
Author(s):  
A.K. Rowan ◽  
G. Moser ◽  
N. Gray ◽  
J.R. Snape ◽  
D. Fearnside ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Direct Sequencing ◽  
Ammonia Oxidizing Bacteria ◽  
Industrial Wastewater Treatment ◽  
Phylogenetic Affiliation ◽  
Gradient Gel Electrophoresis ◽  
Nitrosococcus Mobilis ◽  
Ammonia Oxidising Bacteria

The diversity and community structure of the b-proteobacterial ammonia oxidising bacteria (AOB) in a range of different lab-scale industrial wastewater treatment reactors were compared. Three of the reactors treat waste from mixed domestic and industrial sources whereas the other reactor treats waste solely of industrial origin. PCR with AOB selective primers was combined with denaturing gradient gel electrophoresis to allow comparative analysis of the dominant AOB populations and the phylogenetic affiliation of the dominant AOB was determined by cloning and sequencing or direct sequencing of bands excised from DGGE gels. Different AOB were found within and between different reactors. All AOB sequences identified were grouped within the genus Nitrosomonas. Within the lab-scale reactors there appeared to be selection for a low diversity of AOB and predominance of a single AOB population. Furthermore, the industrial input in both effluents apparently selected for salt tolerant AOB, most closely related to Nitrosococcus mobilis and Nitrosomonas halophila.

Study of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA gene

2001 ◽  
Vol 43 (1) ◽  
pp. 77-82 ◽  
Author(s):  
O.-C. Chan ◽  
W.-T. Liu ◽  
H. H. Fang
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Related Sequence ◽  
Gradient Gel Electrophoresis

The microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). Twelve major bands were observed in the DGGE fingerprint for the Bacteria domain and four bands for the Archaea domain. Of the bacterial bands observed, six were successfully purified and sequenced. Among them, three were related to the gram-positive low G+C group, one to the Delta subclass of the Proteobacteria, one to the Gamma subclass, and one to the Cytophaga group with no close related sequence. The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum.

scholarly journals Molecular Analysis of Ammonia-Oxidizing Bacteria of the β Subdivision of the Class Proteobacteria in Compost and Composted Materials

1999 ◽  
Vol 65 (2) ◽  
pp. 396-403 ◽  
Author(s):  
George A. Kowalchuk ◽  
Zinaida S. Naoumenko ◽  
Piet J. L. Derikx ◽  
Andreas Felske ◽  
John R. Stephen ◽  
...  
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Ammonia Oxidizer ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrogen Transformations ◽  
Rt Pcr ◽  
Gene Encoding ◽  
Specific Pcr ◽  
Pcr Products ◽  
Gradient Gel Electrophoresis ◽  
Almost All

ABSTRACT Although the practice of composting animal wastes for use as biofertilizers has increased in recent years, little is known about the microorganisms responsible for the nitrogen transformations which occur in compost and during the composting process. Ammonia is the principle available nitrogenous compound in composting material, and the conversion of this compound to nitrite in the environment by chemolithotrophic ammonia-oxidizing bacteria is an essential step in nitrogen cycling. Therefore, the distribution of ammonia-oxidizing members of the β subdivision of the class Proteobacteriain a variety of composting materials was assessed by amplifying 16S ribosomal DNA (rDNA) and 16S rRNA by PCR and reverse transcriptase PCR (RT-PCR), respectively. The PCR and RT-PCR products were separated by denaturing gradient gel electrophoresis (DGGE) and were identified by hybridization with a hierarchical set of oligonucleotide probes designed to detect ammonia oxidizer-like sequence clusters in the genera Nitrosospira and Nitrosomonas. Ammonia oxidizer-like 16S rDNA was detected in almost all of the materials tested, including industrial and experimental composts, manure, and commercial biofertilizers. A comparison of the DGGE and hybridization results after specific PCR and RT-PCR suggested that not all of the different ammonia oxidizer groups detected in compost are equally active. amoA, the gene encoding the active-site-containing subunit of ammonia monooxygenase, was also targeted by PCR, and template concentrations were estimated by competitive PCR. Detection of ammonia-oxidizing bacteria in the composts tested suggested that such materials may not be biologically inert with respect to nitrification and that the fate of nitrogen during composting and compost storage may be affected by the presence of these organisms.

Strength characteristics of aerobic granular sludge

2012 ◽  
Vol 65 (2) ◽  
pp. 309-316 ◽  
Author(s):  
A. Nor-Anuar ◽  
Z. Ujang ◽  
M. C. M. van Loosdrecht ◽  
M. K. de Kreuk ◽  
G. Olsson
Keyword(s):  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Strength Analysis ◽  
Anaerobic Granular Sludge ◽  
Aerobic Granules ◽  
Sludge Flocs ◽  
Conventional Activated Sludge ◽  
Biomass Retention ◽  
Settling Characteristic ◽  
Fast Settling

Aerobic granular sludge has a number of advantages over conventional activated sludge flocs, such as cohesive and strong matrix, fast settling characteristic, high biomass retention and ability to withstand high organic loadings, all aspects leading towards a compact reactor system. Still there are very few studies on the strength of aerobic granules. A procedure that has been used previously for anaerobic granular sludge strength analysis was adapted and used in this study. A new coefficient was introduced, called a stability coefficient (S), to quantify the strength of the aerobic granules. Indicators were also developed based on the strength analysis results, in order to categorize aerobic granules into three levels of strength, i.e. very strong (very stable), strong (stable) and not strong (not stable). The results indicated that aerobic granules grown on acetate were stronger (high density: >150 g T SSL−1 and low S value: 5%) than granules developed on sewage as influent. A lower value of S indicates a higher stability of the granules.

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