scholarly journals Glucocorticoid-dependent Tubulointerstitial Nephritis with IgM-positive Plasma Cells Presenting with Intracellular Crystalline Inclusions within the Rough Endoplasmic Reticulum

2021 ◽  
Author(s):  
Masanori Minato ◽  
Taichi Murakami ◽  
Naoki Takahashi ◽  
Hiroyuki Ono ◽  
Kenji Nishimura ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Plasma Cells ◽  
Tubulointerstitial Nephritis ◽  
Crystalline Inclusions

scholarly journals Monoclonal antibody 2H1 detects a 60-65 KD membrane polypeptide of the rough endoplasmic reticulum of neurons and selectively stains cells of several rat tissues.

1991 ◽  
Vol 39 (5) ◽  
pp. 635-643 ◽  
Author(s):  
Y J Chen ◽  
W F Hickey ◽  
S G Mezitis ◽  
A Stieber ◽  
E Lavi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Plasma Cells ◽  
Primary Cultures ◽  
White Pulp ◽  
Rat Tissues ◽  
Pc 12 Cells ◽  
Adrenal Medullary Cells ◽  
Medullary Cells

Monoclonal antibody (MAb) 2H1, raised in mice immunized with membrane fractions from cultured rat pheochromocytoma cells (clonal line PC-12), detects a polypeptide from rat brain and PC-12 cell membranes of 60-65 KD apparent molecular mass. The polypeptide has been localized by immunoelectron microscopy in the rough endoplasmic reticulum (RER) of neurons. By light microscopic immunocytochemistry, several rat tissues and two rat-derived cultured cell types show selective patterns of staining with 2H1. In the central nervous system, the antibody stains neuronal cytoplasm; in the spleen, staining is seen only in certain cells of the marginal zone of the white pulp, and in lymph nodes, in plasma cells, and in areas populated by monocytes and macrophages. Whereas astrocytes and adrenal medullary cells in situ are virtually unstained with 2H1, primary cultures of astrocytes and PC-12 cells, which are derived from adrenal medullary cells, stain intensely with 2H1. The strong staining of cultured astrocytes and PC-12 cells with 2H1 suggests that the levels of the 60-65 KD polypeptide are up-regulated during cell proliferation and growth. Only a few hepatocytes stain with 2H1; intestinal epithelial and pancreatic cells are not stained with 2H1. The organelle-specific antibody 2H1 may prove a useful probe in structural and functional studies of membranes of the rough endoplasmic reticulum in neurons, and in certain cells of the immune system.

Distribution, location, and ultrastructure of plasma cells in the uninfected, lactating bovine mammary gland

1984 ◽  
Vol 51 (2) ◽  
pp. 209-217 ◽  
Author(s):  
Stephen C. Nickerson ◽  
J. Woodrow Pankey ◽  
Nancy T. Boddie
Keyword(s):  
Endoplasmic Reticulum ◽  
Mammary Gland ◽  
Rough Endoplasmic Reticulum ◽  
Plasma Cells ◽  
Progressive Increase ◽  
Cytological Analysis ◽  
Bovine Mammary Gland ◽  
Flocculent Material

SummaryQuantitative cytological analysis demonstrated a marked and progressive increase in concentration of subepithelial plasma cells from milk-secreting parenchyma to the distal teat end mucosa in the normal, lactating bovine mammary gland. Parenchymal plasma cells exhibited typical ultrastructure with abundant, flattened stacks of rough endoplasmic reticulum, and hypertrophied Golgi components, while many cells in the teat end mucosa displayed an ergastoplasm distended with fine, flocculent material. Intraepithelial plasma cells were also observed in teat end mucosa. Results suggest that teat end tissues function by recruiting plasma cells to serve as protection against invading mastitis-causing organisms.

scholarly journals IMMUNOGLOBULIN SYNTHESIS AND SECRETION

1970 ◽  
Vol 46 (1) ◽  
pp. 52-63 ◽  
Author(s):  
Daniel Zagury ◽  
Jonathan W. Uhr ◽  
James D. Jamieson ◽  
George E. Palade
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Electron Microscope ◽  
Rough Endoplasmic Reticulum ◽  
Golgi Complex ◽  
Immunoglobulin G ◽  
Plasma Cells ◽  
Polypeptide Chain ◽  
Polypeptide Backbone ◽  
Mouse Myeloma

The subcellular sites of synthesis and route of intracellular transfer of immunoglobulin G (IgG) have been investigated by electron microscope radioautography with precursors used for the polypeptide chain (leucine-3H) and for the carbohydrate moieties (galactose-3H and glucosamine-3H). For this purpose, plasma cells from a mouse myeloma tumor were labeled with appropriate precursors and the distribution of radioautographic grains was determined at the end of the labeling period and after varying times of incubation in unlabeled medium. The results indicated that the polypeptide backbone is synthesized in a region of the cell occupied by the rough endoplasmic reticulum (RER) and is transported from there to the region of the Golgi complex. Galactose is incorporated in IgG primarily at the level of the Golgi complex, whereas the incorporation of glucosamine appears to take place both in the RER and in the Golgi complex. From the Golgi complex, the completed IgG molecules reach the plasma membrane and are discharged extracellularly. The latter route of transport and the mechanism of discharge are not understood but may be mediated via smooth-surfaced vesicles.

Modification of Pineal Ultrastructure by Exogenous Hormones

1967 ◽  
Vol 25 ◽  
pp. 170-171
Author(s):  
R. A. Turner ◽  
A. E. Rodin ◽  
D. K. Roberts
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
List Type ◽  
Type Number ◽  
Pregnant Rats ◽  
Gonadal Atrophy ◽  
Pineal Ultrastructure

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.

Membrane-associated microtubular areays induced in proximal myelinated processes of dorsal root ganglia by large doses of vitamin B6

1986 ◽  
Vol 44 ◽  
pp. 368-369
Author(s):  
V.J. Montpetit ◽  
S. Dancea ◽  
L. Tryphonas ◽  
D.F. Clapin
Keyword(s):  
Animal Model ◽  
Endoplasmic Reticulum ◽  
Dorsal Root Ganglia ◽  
Rough Endoplasmic Reticulum ◽  
Dorsal Root ◽  
Vitamin B6 ◽  
Morphological Changes ◽  
Model System ◽  
Sensory Nerves ◽  
Peripheral Sensory

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.

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