scholarly journals Chemical Constituents of Snake Fruit (Salacca zalacca (Gaert.) Voss) Peel and in silico Anti-aging Analysis

2019 ◽  
Vol 3 (2) ◽  
pp. 122
Author(s):  
Ermi Girsang ◽  
I Nyoman Ehrich Lister ◽  
Chrismis Novalinda Ginting ◽  
Adrian Khu ◽  
Butter Samin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Palm Tree ◽  
Triterpenoid Saponin ◽  
Fruit Peel ◽  
Wide Range

Background: Skin aging is a condition where skin is unable to retain both its physiological and structural integrity. Plants is the main source of phtytochemicals compound with wide range of biological activities. Through the efforts of ongoing scientific researches, an increasing number of plant extracts and phytochemicals have been showed promising result as anti-aging agent. Snake fruit (Salacca zalacca (Gaert.) Voss) is tropical plant belongs to the palm tree family (Arecaceae) that served as important crop in Indonesia. Despite its utilization, the phytochemical compound available in snake fruit, especially its peel have not been well documented. Present study aimed to elucidate the phytochemical constituent of snake fruit peel and its anti-aging potency.Materials and Methods: Snake fruit peel extract (SPE) was subjected to qualitative phytochemical assay, high performance liquid chromatography, and molecular docking towards protein related in skin aging.Results: The screening showed SPE contained phytochemical compound belong to flavonoid, tannin, phenol, triterpenoid, saponin and alkaloid. Thus, based on the analysis only chlorogenic acid was present in SPE whilst rutin and caffeic acid were not detected. The SPE was contained chlorogenic acid around 1.074 mg/g dry weight. Chlorogenic acid had the high binding affinity towards matrix metalloproteinase (MMP)-1 (-9.4 kcal/mol).Conclusion: Current findings may provide scientific evidence for possible usage of SPE and its compounds as antioxidant and anti-aging agent.Keywords: Salacca zalacca, phytochemical compound, high performance liquid chromatography, anti-aging

scholarly journals Optimasi dan Validasi Metode Kromatografi Cair Kinerja Tinggi untuk Menetapkan Kadar Asam Klorogenat dalam Ekstrak Etanol Daun Yakon (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson)

2020 ◽  
Vol 16 (1) ◽  
pp. 66
Author(s):  
Zuhelmi Aziz ◽  
Liliek Nurhidayati ◽  
Syamsudin Abdillah ◽  
Nancy Dewi Yuliana ◽  
Partomuan Simanjuntak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Biological Activities ◽  
Hplc Method ◽  
Good Precision ◽  
Smallanthus Sonchifolius ◽  
Relative Standard

<p><span id="docs-internal-guid-c5cb41fa-7fff-bcb7-a369-034e4910c35f"><span>Yakon merupakan tanaman yang dapat digunakan untuk pengobatan dan kebutuhan pangan. Salah satu kandungan zat berkhasiat dalam daun yakon adalah asam klorogenat. Asam klorogenat diketahui memiliki aktifitas sebagai antioksidan, antikanker dan antidiabetes.  Penentuan kadar asam klorogenat dalam matriks yang kompleks diperlukan metode yang selektif dengan ketelitian dan ketepatan yang baik. Pada penelitian ini dilakukan optimasi dan validasi metode kromatografi cair kinerja tinggi (KCKT) fase balik untuk penetapan kadar asam klorogenat. Ekstrak dibuat secara ultrasonikasi menggunakan pelarut etanol 95%. Kondisi optimum diperoleh menggunakan fase gerak asam format 0,1% dalam asetonitril–asam format 0,1% dalam air (gradien); fase diam oktadesilsilan (C</span><span><span>18</span></span><span>) pada suhu 30 ºC dan detektor UV pada panjang gelombang 328 nm. Metode KCKT ini memberikan hasil yang memiliki ketelitian yang tinggi dengan simpangan baku relatif 0,79% dan ketepatan yang baik dengan perolehan kembali 97,50%. Kadar asam klorogenat yang diperoleh dalam ekstrak etanol 95% daun yakon sebesar 1,02%.</span></span></p><p> </p><p dir="ltr"><span>Optimization and Validation of High Performance Liquid Chromatography Methods for Determination of Chlorogenic Acid Levels in Ethanol Extracts of Yakon Leaves (</span><span>Smallanthus sonchifolius</span><span> (Poepp. &amp; Endl.) H. Robinson). </span><span>Yacon is a plant that can be used for medication and food needs. One of the bioactive compounds of yacon leaves is chlorogenic acid. Chlorogenic acid has various biological activities, such as antioxidant, anticancer and antidiabetic activities. </span><span>To determine the chlorogenic acid in such complex matrix, such selective methods with good precision and acuracy are required.</span><span> In this study, the optimization and validation of reverse phase high performance liquid chromatography (HPLC) method for chlorogenic acid determination </span><span>were performed</span><span>. The extract was prepared by ultrasonication in 95% ethanol</span><span>.</span><span>The optimized condition for HPLC </span><span>obtained was by using</span><span> mobile phase  0.1% formic acid in acetonitrile – 0.1% formic acid in water with gradient elution, stationary phase octadesylsilane  (C</span><span><span>18</span></span><span>)  at 30 </span><span><span>o</span></span><span>C and UV detector at of 328 nm. The result showed that HPLC method had high precicion with relative standard deviation of 0.79% and high accuracy with recovery of 97.50%. The chlorogenic acid in the ethanol 95% extract of </span><span>Y</span><span>acon leaves was 1,02%.</span></p><div><span><br /></span></div>

scholarly journals Optimasi dan Validasi Metode Kromatografi Cair Kinerja Tinggi untuk Menetapkan Kadar Asam Klorogenat dalam Ekstrak Etanol Daun Yakon (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson)

2020 ◽  
Vol 16 (1) ◽  
pp. 67
Author(s):  
Zuhelmi Aziz ◽  
Liliek Nurhidayati ◽  
Syamsudin Abdillah ◽  
Nancy Dewi Yuliana ◽  
Partomuan Simanjuntak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Biological Activities ◽  
Hplc Method ◽  
Good Precision ◽  
Smallanthus Sonchifolius ◽  
Relative Standard

<p><span id="docs-internal-guid-c5cb41fa-7fff-bcb7-a369-034e4910c35f"><span>Yakon merupakan tanaman yang dapat digunakan untuk pengobatan dan kebutuhan pangan. Salah satu kandungan zat berkhasiat dalam daun yakon adalah asam klorogenat. Asam klorogenat diketahui memiliki aktifitas sebagai antioksidan, antikanker dan antidiabetes.  Penentuan kadar asam klorogenat dalam matriks yang kompleks diperlukan metode yang selektif dengan ketelitian dan ketepatan yang baik. Pada penelitian ini dilakukan optimasi dan validasi metode kromatografi cair kinerja tinggi (KCKT) fase balik untuk penetapan kadar asam klorogenat. Ekstrak dibuat secara ultrasonikasi menggunakan pelarut etanol 95%. Kondisi optimum diperoleh menggunakan fase gerak asam format 0,1% dalam asetonitril–asam format 0,1% dalam air (gradien); fase diam oktadesilsilan (C</span><span><span>18</span></span><span>) pada suhu 30 ºC dan detektor UV pada panjang gelombang 328 nm. Metode KCKT ini memberikan hasil yang memiliki ketelitian yang tinggi dengan simpangan baku relatif 0,79% dan ketepatan yang baik dengan perolehan kembali 97,50%. Kadar asam klorogenat yang diperoleh dalam ekstrak etanol 95% daun yakon sebesar 1,02%.</span></span></p><p> </p><p dir="ltr"><span>Optimization and Validation of High Performance Liquid Chromatography Methods for Determination of Chlorogenic Acid Levels in Ethanol Extracts of Yakon Leaves (</span><span>Smallanthus sonchifolius</span><span> (Poepp. &amp; Endl.) H. Robinson). </span><span>Yacon is a plant that can be used for medication and food needs. One of the bioactive compounds of yacon leaves is chlorogenic acid. Chlorogenic acid has various biological activities, such as antioxidant, anticancer and antidiabetic activities. </span><span>To determine the chlorogenic acid in such complex matrix, such selective methods with good precision and acuracy are required.</span><span> In this study, the optimization and validation of reverse phase high performance liquid chromatography (HPLC) method for chlorogenic acid determination </span><span>were performed</span><span>. The extract was prepared by ultrasonication in 95% ethanol</span><span>.</span><span>The optimized condition for HPLC </span><span>obtained was by using</span><span> mobile phase  0.1% formic acid in acetonitrile – 0.1% formic acid in water with gradient elution, stationary phase octadesylsilane  (C</span><span><span>18</span></span><span>)  at 30 </span><span><span>o</span></span><span>C and UV detector at of 328 nm. The result showed that HPLC method had high precicion with relative standard deviation of 0.79% and high accuracy with recovery of 97.50%. The chlorogenic acid in the ethanol 95% extract of </span><span>Y</span><span>acon leaves was 1,02%.</span></p><div><span><br /></span></div>

scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

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