mazE Antitoxin of Toxin Antitoxin System and fbpA as Reliable Targets to Eradication of Neisseria meningitidis

2018 ◽  
Vol 24 (11) ◽  
pp. 1204-1210 ◽  
Author(s):  
Abbas Maleki ◽  
Sobhan Ghafourian ◽  
Iraj Pakzad ◽  
Behzad Badakhsh ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Nucleic Acid ◽  
Neisseria Meningitidis ◽  
Peptide Nucleic Acid ◽  
Iron Uptake ◽  
Drug Target ◽  
Uptake System ◽  
Bacteriostatic Activity ◽  
In Vivo Analysis ◽  
Antisense Peptide

Background: Neisseria meningitidis is considered as a dangerous pathogen threatening human health. Nowadays, the new drug target is focused. Toxin antitoxin (TA) system is recently identified as an antimicrobial drug target. Also, in N. meningitidis, iron-uptake system could be an interesting target for drug discovery. Methods: In this study, fbpA and mazE genes were chosen as new antimicrobial targets and treated with antisense peptide nucleic acid (PNA). Firstly, they were evaluated by bioinformatics and then analyzed by experimental procedures. Secondly, the functionality was evaluated by stress conditions. Results: Our results interestingly demonstrated that when fbpA and mazE loci of N. meningitidis were targeted by antisense PNA, 8 µM concentration of fbpA-PNA as well as 30 µM concentration of mazE-PNA inhibited the growth of N. meningitides and were found to be bacteriostatic, whereas 10 μM concentration of fbpA-PNA showed bacteriocidal activity. Conclusion: Our findings demonstrated the bactriocidal activity of fbpA-PNA and bacteriostatic activity of mazEPNA. Therefore, mazE and fbpA genes should be potent antimicrobial targets but further analysis including in vivo analysis should be performed.

scholarly journals Antimicrobial activity of antisense peptide–peptide nucleic acid conjugates against non-typeableHaemophilus influenzaein planktonic and biofilm forms

2016 ◽  
Vol 72 (1) ◽  
pp. 137-144 ◽  
Author(s):  
Taketo Otsuka ◽  
Aimee L. Brauer ◽  
Charmaine Kirkham ◽  
Erin K. Sully ◽  
Melinda M. Pettigrew ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Antisense Peptide

scholarly journals Detection and identification of specific bacteria in wound biofilms using peptide nucleic acid fluorescent in situ hybridization (PNA FISH)

Microbiology ◽  
2009 ◽  
Vol 155 (8) ◽  
pp. 2603-2611 ◽  
Author(s):  
Sladjana Malic ◽  
Katja E. Hill ◽  
Anthony Hayes ◽  
Steven L. Percival ◽  
David W. Thomas ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Bacterial Species ◽  
Bacterial Load ◽  
Bacterial Biofilm ◽  
Laser Scanning Microscopy ◽  
Individual Species ◽  
Fish Analysis

Biofilms provide a reservoir of potentially infectious micro-organisms that are resistant to antimicrobial agents, and their importance in the failure of medical devices and chronic inflammatory conditions is increasingly being recognized. Particular research interest exists in the association of biofilms with wound infection and non-healing, i.e. chronic wounds. In this study, fluorescent in situ hybridization (FISH) was used in combination with confocal laser scanning microscopy (CLSM) to detect and characterize the spatial distribution of biofilm-forming bacteria which predominate within human chronic skin wounds (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus sp. and Micrococcus sp.). In vitro biofilms were prepared using a constant-depth film fermenter and a reconstituted human epidermis model. In vivo biofilms were also studied using biopsy samples from non-infected chronic venous leg ulcers. The specificity of peptide nucleic acid (PNA) probes for the target organisms was confirmed using mixed preparations of planktonic bacteria and multiplex PNA probing. Identification and location of individual bacterial species within multi-species biofilms demonstrated that P. aeruginosa was predominant. CLSM revealed clustering of individual species within mixed-species biofilms. FISH analysis of archive chronic wound biopsy sections showed bacterial presence and allowed bacterial load to be determined. The application of this standardized procedure makes available an assay for identification of single- or multi-species bacterial populations in tissue biopsies. The technique provides a reliable tool to study bacterial biofilm formation and offers an approach to assess targeted biofilm disruption strategies in vivo.

Approaches for Systemic Delivery of Dystrophin Antisense Peptide Nucleic Acid in the mdx Mouse Model

2020 ◽  
Author(s):  
Camilla Brolin ◽  
Ernest Wee Kiat Lim ◽  
Sylvestre Grizot ◽  
Caroline Holkmann Olsen ◽  
Niloofar Yavari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Mouse Model ◽  
Peptide Nucleic Acid ◽  
Mdx Mouse ◽  
Systemic Delivery ◽  
Antisense Peptide

147: Intra Vesical Admininstration of Antisense Peptide Nucleic Acid Down Regulates Urothelial NGF Expression in Rat Model of Cystitis

2005 ◽  
Vol 173 (4S) ◽  
pp. 40-40
Author(s):  
Pradeep Tyagi ◽  
Michael B. Chancellor ◽  
Naoki Yoshimura ◽  
Leaf Huang
Keyword(s):  
Nucleic Acid ◽  
Rat Model ◽  
Peptide Nucleic Acid ◽  
Antisense Peptide
Sign in / Sign up

Export Citation Format

Share Document