scholarly journals Trichoderma reesei CRE1-mediated Carbon Catabolite Repression in Response to Sophorose Through RNA Sequencing Analysis

2016 ◽  
Vol 17 (2) ◽  
pp. 119-131 ◽  
Author(s):  
Amanda Cristina Campos Antoniêto ◽  
Renato Graciano de Paula ◽  
Lílian dos Santos Castro ◽  
Rafael Silva-Rocha ◽  
Gabriela Felix Persinoti ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Sequencing Analysis

Carbon catabolite repression of xylanase I (xyn1) gene expression in Trichoderma reesei

1996 ◽  
Vol 21 (6) ◽  
pp. 1273-1281 ◽  
Author(s):  
Robert L. Mach ◽  
Joseph Strauss ◽  
Susanne Zeilinger ◽  
Martin Schindler ◽  
Christian P. Kubicek
Keyword(s):  
Gene Expression ◽  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression

scholarly journals Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Nozomu Shibata ◽  
Hiroshi Kakeshita ◽  
Kazuaki Igarashi ◽  
Yasushi Takimura ◽  
Yosuke Shida ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Enzyme Production ◽  
Protein Production ◽  
Carbon Catabolite Repression ◽  
Transcriptional Regulator ◽  
Secretory Protein ◽  
Sugar Transporter ◽  
Alpha Tubulin ◽  
High Enzyme

Abstract Background Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon catabolite repression (CCR) has long been a problem. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. Results We found that deletion of an α-tubulin (tubB) in T. reesei enhanced both the amount and rate of secretory protein production. Also, the tubulin-disrupted (ΔtubB) strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. From transcriptome analysis, the ΔtubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the ΔtubB strain. These results suggested that the ΔtubB strain was released from CCR. Conclusion Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. reesei. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. reesei.

Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis

2014 ◽  
Vol 73 ◽  
pp. 93-103 ◽  
Author(s):  
Amanda Cristina Campos Antoniêto ◽  
Lílian dos Santos Castro ◽  
Rafael Silva-Rocha ◽  
Gabriela Felix Persinoti ◽  
Roberto Nascimento Silva
Keyword(s):  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Rna Seq ◽  
Genome Wide

scholarly journals Genetic Modification of Carbon Catabolite Repression in Trichoderma reesei for Improved Protein Production

2009 ◽  
Vol 75 (14) ◽  
pp. 4853-4860 ◽  
Author(s):  
Tiina Nakari-Setälä ◽  
Marja Paloheimo ◽  
Jarno Kallio ◽  
Jari Vehmaanperä ◽  
Merja Penttilä ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Enzyme Production ◽  
Null Allele ◽  
Carbon Catabolite Repression ◽  
Regulatory Gene ◽  
Hydrolytic Enzymes ◽  
Strain Engineering ◽  
Mutant Variant ◽  
Mutant Strains

ABSTRACT The cellulase and hemicellulase genes of the filamentous fungus Trichoderma reesei have been shown to be under carbon catabolite repression mediated by the regulatory gene cre1. In this study, strains were constructed in which the cre1 gene was either completely removed or replaced by a truncated mutant variant, cre1-1, found previously in the Rut-C30 mutant strain with enhanced enzyme production capability. The T. reesei transformants with either deletion or truncation of cre1 had clearly altered colony morphology compared with the parental strains, forming smaller colonies and fewer aerial hyphae and spores. Liquid cultures in a medium with glucose as a carbon source showed that the transformants were derepressed in cellulase and hemicellulase production. Interestingly, they also produced significantly elevated levels of these hydrolytic enzymes in fermentations carried out in a medium inducing the hydrolase genes. This suggests that cre1 acts as a modulator of cellulase and hemicellulase gene expression under both noninducing and inducing conditions. There was no phenotypic difference between the Δcre1 and cre1-1 mutant strains in any of the experiments done, indicating that the cre1-1 gene is practically a null allele. The results of this work indicate that cre1 is a valid target gene in strain engineering for improved enzyme production in T. reesei.

scholarly journals Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei

2020 ◽  
Vol 5 (3) ◽  
pp. 230-235 ◽  
Author(s):  
Lijuan Han ◽  
Kuimei Liu ◽  
Wei Ma ◽  
Yi Jiang ◽  
Shaoli Hou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression

scholarly journals The molecular taxonomy of primate amygdala via single-nucleus RNA-sequencing analysis

2021 ◽  
Author(s):  
Lei Zhang ◽  
Yanyong Cheng ◽  
Shihao Wu ◽  
Yufeng Lu ◽  
Zhenyu Xue ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Molecular Taxonomy ◽  
Sequencing Analysis ◽  
Single Nucleus

scholarly journals CTIM-06. DENDRITIC CELL VACCINE STUDY FOR RECURRENT, HIGH-GRADE GLIOMA: TISSUE-BASED RNA SEQUENCING AND SERUM CYTOKINE LEVELS AS POTENTIAL BIOMARKERS

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii33-ii34
Author(s):  
Macarena De La Fuente ◽  
Tulay Koru-Sengul ◽  
Deborah Heros ◽  
Feng Miao ◽  
Alain Fernandez Marrero ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Rna Sequencing ◽  
Tumor Antigens ◽  
High Grade Glioma ◽  
Treatment Discontinuation ◽  
Sequencing Analysis ◽  
High Grade ◽  
Who Grade ◽  
Cytokine Levels ◽  
Grade 3

Abstract BACKGROUND Glioblastoma is the most common primary malignant brain tumor. Despite multimodality treatment approach, median progression-free survival (PFS) is only 8 months, median overall-survival (OS) 14 months and 5-year survival rate of under 10%. Dendritic cells (DCs) are the professional antigen presenting cells of the immune system. The rationale for sensitizing dendritic cells to a pool of non-selected tumor antigens is based on the marked heterogeneity present within glioblastoma tumor cells. METHODS Phase 1/feasibility study of DC vaccine for recurrent high-grade glioma was conducted. Pooled, non-selected tumor antigens collected via tumor cell lysate were used for DC sensitization. RNA sequencing analysis was performed on all tumor samples. Cytokine levels in serum were detected using a Luminex cytokine panel. RESULTS A total of 20 patients were enrolled onto this study (median age 58yrs, range: 39–74, 65% male). Pathology showed WHO grade IV glioblastoma in 14 (70%) and grade III anaplastic astrocytoma in 6 (30%) patients. IDH wild type in 19 (95%) patients. Treatment emergent adverse events (all grades, regardless of attribution) occurred in more than 15% of the patients (20% fatigue, 15% dizziness, 15% headache, none leading to treatment discontinuation). There were five grade 3–4 and none grade 5 events. One grade 4 event (seizure) probable related to investigational treatment leading to treatment discontinuation. Four grade 3 events (dysphasia, possible related; intracranial hemorrhage unrelated; muscle weakness, unlikely related and hematoma, unrelated). Median PFS was 3.8 months. Median OS was 11 months. RNA sequencing in tumor samples and correlation with cytokine levels in serum is currently been analyzed. CONCLUSION Tumor lysate pulsed DC vaccination demonstrates acceptable safety and tolerability in high-grade glioma patients. Evaluations of integrating molecular profiling RNA sequencing information and cytokine levels to identify potential subset of patients with significant clinical benefit will be provided.

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