Chemicals Constituents of the Algerian Glycyrrhiza glabra L. Root Extracts and their Antioxidant, Antibacterial and Anticholinesterase Activities

2020 ◽  
Vol 16 (8) ◽  
pp. 1215-1224
Author(s):  
Saïd Barek ◽  
Mohammed N. Rahmoun ◽  
Mohammed Aissaoui ◽  
Chawki Bensouici ◽  
Imad A. El Haci ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
1H Nmr ◽  
Radical Scavenging ◽  
Glycyrrhiza Glabra ◽  
Hydrogen Atoms ◽  
Standard Drug ◽  
Root Extracts ◽  
Enzymes Inhibition ◽  
Glycyrrhiza Glabra L ◽  
Different Strains

Background: Glycyrrhiza glabra L. is an important medicinal plant endowed with various pharmacological virtues. This study aimed to investigate the antioxidant, antibacterial, and anticholinesterase activities of the Algerian Glycyrrhiza glabra L. roots extracts. Methods: The chemical composition of both chloroformic (LCh) and ethyl acetate (LAE) extracts were analyzed by RP-HPLC-PDA and 1H NMR spectroscopy. The antioxidant activity was evaluated using hydrogen atoms transfer methods (DPPH) and single electron transfer (ABTS and CUPRAC assays). The antibacterial activity was realized against different strains via the Minimal Inhibitory Concentration (MIC), when the anticholinesterase activity was performed through the acetylcholinesterase and butyrylcholinesterase enzymes inhibition. Results: The chemical analysis revealed the presence of phenolic acids (gallic acid, p-coumaric acid) and a hydroxycinnamic compound (ferulic acid). However, flavonoids were represented by quercetin, rutin (flavonol), and glabridin (isoflavane). The 1H NMR of the L4 fraction from LCh extract allowed to characterize the structure of glabridin. : The antioxidant assays revealed that LCh extract is the best among other extracts with IC50 DPPH of 33.94 μg/mL, IC50 ABTS of 3.45 μg/mL and CUPRAC A0.5 of 21.78 μg/mL. The LCh extract displayed an effective antibacterial activity with MIC’s of 19.5 μg/mL against seven gram positive and negative bacteria strains. The same extract showed a potent butyrylcholinesterase inhibitory activity with IC50= 4.72 ± 0.72 μg/mL, which is too strong than the standard drug. Conclusion: The study demonstrated that G. glabra root extracts had a high antibacterial, and free radical scavenging. It was also able to inhibit cholinesterase enzymes, which confirm the effectiveness of phytoconstituents present in the plant, especially flavonoids.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF ROOT EXTRACTS OF GLYCYRRHIZA GLABRA L

2016 ◽  
Vol 9 (6) ◽  
pp. 85
Author(s):  
Archana Sakthi
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Glycyrrhiza Glabra ◽  
Peroxide Radical ◽  
Scavenging Activity ◽  
Root Extracts ◽  
Nitric Oxide Radical ◽  
Glycyrrhiza Glabra L

ABSTRACT:     Obesity is an international health problem caused by various environmental and genetic factors. Herbal supplements have proved to be active against obesity. Glycyrrhiza glabra belongs to the family of Fabaceae. Glycyrrhiza glabra was used traditionally to treat various diseases. Hence the current study was planned to investigate the phytochemicals and antioxidants present in Glycyrrhiza glabra. Materials and Methods: The preliminary qualitative, quantitative phytochemicals and antioxidants were investigated from the root extracts of Glycyrrhiza glabra. Results: The preliminary qualitative analysis of various extracts of Glycyrrhiza glabra showed the presence of Phenols, Flavonoids, Tannins, Saponins and Quinones. Quantitative analysis of Glycyrrhiza glabra showed elevated amounts of phenols, flavonoids and tannins in ethanolic extracts. The Aqueous, ethanol, hydro alcoholic extracts of Glycyrrhiza glabra showed 211±2.64, 281.66±1.57, 263±14.2 mg of phenols, 152.66±3.05, 187.33±1.52, 183.33±3.05 mg of flavonoids, 21.33±0.57, 41±3.46, 34.3±1.15 mg of Tannins, 42.33±2.05, 63.33±1.52, 36.33±1.57 mg of terpenoids. The antioxidant properties of the ethanolic extract of Glycyrrhiza glabra were evaluated with the 2,2-diphenyl-1-picryl hydrazyl (DPPH), Nitric Oxide radical (NO), Superoxide radical (SO), Hydrogen peroxide radical (H2O2), Hydroxyl radical (HO) Scavenging activity. The IC50 value of Glycyrrhiza glabra is 120 µg/ml for DPPH, 208 µg/ml for (NO), 196 µg/ml for (SO), 148 µg/ml for (H2O2), and 252 µg/ml for (OH). Invitro method suggests that Glycyrrhiza glabra is more powerful against nitric oxide and hydroxyl radicals. Conclusion: This preliminary study shows that Glycyrrhiza glabra is a promising herb with many phytochemicals and antioxidants it can be further investigated for its antiobesity potential.Key words: Glycyrrhiza glabra L., phytochemicals, antioxidant and obesity.

scholarly journals Potential of Antibacterial Compounds from Sweet Root Extract (Glycyrrhiza glabra L) on Bacillus cereus

2019 ◽  
Vol 22 (4) ◽  
pp. 105-111
Author(s):  
Gandi Sogandi ◽  
Wan Syurya Tri Darma ◽  
Raudatul Jannah
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Bioactive Compounds ◽  
Glycyrrhiza Glabra ◽  
Diffusion Method ◽  
Root Extract ◽  
Antibacterial Compounds ◽  
Herbal Plants ◽  
Glycyrrhiza Glabra L

The high number of poisoning food and diarrheal diseases caused by Bacillus cereus bacteria has been treated with chemical drugs and traditionally herbal plants. One of the commonly used herbal plants is sweet root (Glycyrrhiza glabra L.). This study aims to extract and fractionate sweet root plants, determine the antibacterial activity and identify the types of bioactive compounds as antibacterial compounds. The extraction process uses a maceration technique and fractionation using buthanol, ethyl acetate, and hexan solvents. Antibacterial activity was carry out by the diffusion method and identification of bioactive compounds by GCMS analysis. This study showed that greatest antibacterial activity was found in ethyl acetate fraction with a strong category and MIC value of 12,5%. This study for the first time also revealed that the types of bioactive compounds from sweet root plants (Glycyrrhiza glabra L.) as antibacterial compounds are n-Hexadecanoic as a fatty acid group and 4H-Pyran 4-one 2.3 dihydro-3, 5-dihydroxy-6 methyl from the flavonoid group.

Free radical-scavenging, antioxidant and immunostimulating effects of a licorice infusion (Glycyrrhiza glabra L.)

2010 ◽  
Vol 122 (3) ◽  
pp. 508-517 ◽  
Author(s):  
José Cheel ◽  
Pierre Van Antwerpen ◽  
Lenka Tůmová ◽  
Gabriela Onofre ◽  
Doris Vokurková ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Glycyrrhiza Glabra ◽  
Glycyrrhiza Glabra L

scholarly journals STUDY OF POTENTIAL ANTITUSSIVE ACTIVITY OF GLYCYRRHIZA GLABRA GRANULES BY USING A COUGH MODEL INDUCED BY SULPHUR DIOXIDE GAS IN MICE

2019 ◽  
pp. 262-267
Author(s):  
Mayuri Shitole ◽  
Vijaykumar Pawar
Keyword(s):  
Body Weight ◽  
Sulphur Dioxide ◽  
Glycyrrhiza Glabra ◽  
Control Group ◽  
Common Symptom ◽  
Standard Drug ◽  
Cough Reflex ◽  
Glycyrrhiza Glabra L ◽  
Antitussive Activity ◽  
Respiratory Depressant

In respiratory diseases cough is most common symptom. When cough becomes severe, opioids are potent, but they have side effects like sedation, constipation. Therefore, there is a necessary to have effective antitussive formulation, which does not have respiratory depressant activity. The present study was carried out to analysis antitussive activity of Glycyrrhiza glabra L. using a cough model induced by sulphur dioxide gas in experimental mice. The effect of the granules of Glycyrrhiza glabra extract on SO2 gas induced cough in experimental animals have very significant effects at the level of p<0.01 in inhibiting the cough reflex at a dose of 200 mg/kg body weight, in comparison with the control group. Mice were showed an inhibition of 41.17%, in cough on treatment with Glycyrrhiza glabra granules at 60 min experiment. The antitussive activity of the granules was comparable to that of codeine sulphate (10, 15, 20 mg/kg body weight), a standard anti-tussive agent. Codeine sulphate, as a standard drug for suppression of cough, produced 25.29%, 33.33%, 47.13% inhibition in cough at a dose of 10 mg/kg, 15 mg/kg and 20 mg/kg respectively, whereas, codeine sulphate (20 mg/kg) showed maximum 47.13% (p<0.001) inhibition at 60 min of the experiment.

Synthesis, Characterization and Evaluation of Azetidinylquinolines and Thiazolidinylquinolines as Antibacterial Agents

2014 ◽  
Vol 7 (3) ◽  
pp. 2536-2543
Author(s):  
Shalabh Sharma ◽  
Kuldeep K Saxena
Keyword(s):  
Antibacterial Activity ◽  
Spectral Data ◽  
1H Nmr ◽  
Elemental Analysis ◽  
Antibacterial Agents ◽  
Mass Spectral Data ◽  
Standard Drug ◽  
Mass Spectral ◽  
New Compounds

Several molecules were synthesized like 8-ethoxy-4-methyl-2-amino-(3’-chloro-2’-oxo-4’-substitutedaryl-1’-azeti-dinyl)-quinolines 8-12 and 8-ethoxy-4-methyl-2-amino-(2’-substitutedaryl-4’-oxo-1’,3’-thiazolidin-3’-yl)quinolines 13-17 from 8-ethoxy-4-methyl-2-(substitutedarylidenylimino amino)-quinolines 3-7, and screened to evaluate their antibacterial activity against numerous strains of bacteria        in vitro. The structures of new compounds were established on the basis of their elemental analysis, IR, 1H-NMR and mass spectral data. The results showed that three compounds 10, 11 and 16 were found to exhibit promising antibacterial activity as compared to the standard drug amphicillin.   

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