scholarly journals Micro Soft Tissues Visualization Based on X-Ray Phase-Contrast Imaging

2011 ◽  
Vol 5 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Lu Zhang ◽  
Shuqian Luo
Keyword(s):  
Phase Contrast ◽  
Soft Tissues ◽  
Early Stage ◽  
Blood Clot ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Non Invasive

The current imaging methods have a limited ability to visualize microstructures of biological soft tissues. Small lesions cannot be detected at the early stage of the disease. Phase contrast imaging (PCI) is a novel non-invasive imaging technique that can provide high contrast images of soft tissues by the use of X-ray phase shift. It is a new choice in terms of non-invasively revealing soft tissue details. In this study, the lung and hepatic fibrosis models of mice and rats were used to investigate the ability of PCI in microstructures observation of soft tissues. Our results demonstrated that different liver fibrosis stages could be distinguished non-invasively by PCI. The three-dimensional morphology of a segment of blood vessel was constructed. Noteworthy, the blood clot inside the vessel was visualized in three dimensions which provided a precise description of vessel stenosis. Furthermore, the whole lung airways including the alveoli were obtained. We had specifically highlighted its use in the visualization and assessment of the alveoli. To our knowledge, this was the first time for non-invasive alveoli imaging using PCI. This finding may offer a new perspective on the diagnosis of respiratory disease. All the results confirmed that PCI will be a valuable tool in biological soft tissues imaging.

scholarly journals Assisted walking in Malagasy dwarf chamaeleons

2010 ◽  
Vol 6 (6) ◽  
pp. 740-743 ◽  
Author(s):  
Renaud Boistel ◽  
Anthony Herrel ◽  
Gheylen Daghfous ◽  
Paul-Antoine Libourel ◽  
Elodie Boller ◽  
...  
Keyword(s):  
Inner Ear ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Unique Mode ◽  
Morphological Adaptations ◽  
Long Tails

Chamaeleons are well known for their unique suite of morphological adaptations. Whereas most chamaeleons are arboreal and have long tails, which are used during arboreal acrobatic manoeuvres, Malagasy dwarf chamaeleons ( Brookesia ) are small terrestrial lizards with relatively short tails. Like other chamaeleons, Brookesia have grasping feet and use these to hold on to narrow substrates. However, in contrast to other chamaeleons, Brookesia place the tail on the substrate when walking on broad substrates, thus improving stability. Using three-dimensional synchrotron X-ray phase-contrast imaging, we demonstrate a set of unique specializations in the tail associated with the use of the tail during locomotion. Additionally, our imaging demonstrates specializations of the inner ear that may allow these animals to detect small accelerations typical of their slow, terrestrial mode of locomotion. These data suggest that the evolution of a terrestrial lifestyle in Brookesia has gone hand-in-hand with the evolution of a unique mode of locomotion and a suite of morphological adaptations allowing for stable locomotion on a wide array of substrates.

scholarly journals Three-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron RadiationThree-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

2020 ◽  
Author(s):  
Qiang Tao ◽  
Chen-Chen Gao ◽  
Xue-Hong Tong ◽  
Shizhen Yuan ◽  
Tian-tian Wang ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Image Resolution ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Rat Stomach ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional

Abstract Objectives This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using X-ray in-line phase-contrast imaging (XILPCI). The aim of the study was to demonstrate that XILPCI is a micron imaging method for gastric structures. Methods The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution. Results The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastroduodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future. Keywords: Synchrotron radiation phase-contrast imaging, 3-dimensional gastric structure images

X-ray synchrotron phase contrast imaging for structural analysis of soft tissues and biomaterials

2019 ◽  
Author(s):  
B. Singh ◽  
A. K. Agrawal ◽  
Y. S. Kashyap ◽  
M. Shukla ◽  
B. N. Pandey ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Phase Contrast ◽  
Soft Tissues ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray

The Research of Cancer Imaging by X-Ray In-Line Phase Contrast Imaging Technique

2013 ◽  
Vol 718-720 ◽  
pp. 2099-2102
Author(s):  
Qiang Tao ◽  
Shu Qian Luo
Keyword(s):  
Nude Mice ◽  
Phase Contrast ◽  
Soft Tissues ◽  
Ccd Camera ◽  
Biological Tissues ◽  
Image Resolution ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
Noninvasive Technique ◽  
X Ray

The hard X-ray in-line phase contrast imaging (HXILPCI) is a phase contrast technique that generates excellent contrast of biological soft tissues compared to conventional X-ray absorption radiography. We explore the application of HXILPCI in the diagnosis of gastric cancer and pancreatic cancer. These nude mice cancer samples were checked by HXILPCI to obtain projection contrast images of 9μm image resolution with CCD camera. The texture extraction was based on gray level co-occurrence matrix (GLCM). The corresponding morphological features of abnormal and normal tissues are analyzed. The produced phase contrast images of nude mice cancer samples show clearly biological tissues architectures and the size of cancer. The paper results show that HXILPCI can be a potential noninvasive technique to diagnose early cancer.

scholarly journals Towards tender X-rays with Zernike phase-contrast imaging of biological samples at 50 nm resolution

2014 ◽  
Vol 21 (4) ◽  
pp. 790-794 ◽  
Author(s):  
Ismo Vartiainen ◽  
Martin Warmer ◽  
Dennis Goeries ◽  
Eva Herker ◽  
Rudolph Reimer ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
Phase Contrast Imaging ◽  
X Rays ◽  
Contrast Imaging ◽  
Full Field ◽  
X Ray ◽  
Lipid Droplet Formation ◽  
Contrast Microscope ◽  
Science Application

X-ray microscopy is a commonly used method especially in material science application, where the large penetration depth of X-rays is necessary for three-dimensional structural studies of thick specimens with high-Zelements. In this paper it is shown that full-field X-ray microscopy at 6.2 keV can be utilized for imaging of biological specimens with high resolution. A full-field Zernike phase-contrast microscope based on diffractive optics is used to study lipid droplet formation in hepatoma cells. It is shown that the contrast of the images is comparable with that of electron microscopy, and even better contrast at tender X-ray energies between 2.5 keV and 4 keV is expected.

scholarly journals Influence of Data Acquisition Algorithms on X-Ray Phase Contrast Imaging Computed Tomography

2020 ◽  
Vol 3 (4) ◽  
Author(s):  
Collin J. C. Epstein ◽  
Ryan N. Goodner ◽  
R. Derek West ◽  
Kyle R. Thompson ◽  
Amber L. Dagel
Keyword(s):  
Computed Tomography ◽  
Data Acquisition ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Quality Data ◽  
Phase Contrast Imaging ◽  
Ct Reconstruction ◽  
Contrast Imaging ◽  
X Ray ◽  
Phase Stepping

Abstract X-ray phase contrast imaging (XPCI) is a nondestructive evaluation technique that enables high-contrast detection of low-attenuation materials that are largely transparent in traditional radiography. Extending a grating-based Talbot-Lau XPCI system to three-dimensional imaging with computed tomography (CT) imposes two motion requirements: the analyzer grating must translate transverse to the optical axis to capture image sets for XPCI reconstruction, and the sample must rotate to capture angular data for CT reconstruction. The acquisition algorithm choice determines the order of movement and positioning of the two stages. The choice of the image acquisition algorithm for XPCI CT is instrumental to collecting high fidelity data for reconstruction. We investigate how data acquisition influences XPCI CT by comparing two simple data acquisition algorithms and determine that capturing a full phase-stepping image set for a CT projection before rotating the sample results in higher quality data.

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