Green-synthesized Zinc Oxide Nanoparticles from Aqueous Root Extract of Dicoma anomala (Sond.) Mitigates Free Radicals and Diabetes-linked Enzymes

2020 ◽  
Vol 10 (6) ◽  
pp. 918-929
Author(s):  
F.O. Balogun ◽  
A.O.T. Ashafa
Keyword(s):  
Zinc Oxide ◽  
Free Radicals ◽  
Zinc Oxide Nanoparticles ◽  
Alpha Amylase ◽  
Oxide Nanoparticles ◽  
Root Extract ◽  
Metal Chelating ◽  
Transmission Electron ◽  
Hydrolyzing Enzymes ◽  
Alpha Glucosidase

Background: The emergence of eco-friendly methods for the synthesis of metallic nanostructures has continued to receive wider acceptance. Objective: The study investigated the effect of biologically-synthesized ZnO nanoparticles on free radicals and carbohydrate-hydrolyzing enzymes. Methods: The characterized nanoparticles, DaZnONPs (Dicoma anomala zinc oxide nanoparticles) were obtained using ultraviolet-visible spectroscopy, transmission electron microscopy, Fourier- transform infrared spectroscopy (FTIR), energy dispersive spectroscopy, and X-ray diffraction technique (XRD). The activity of the synthesized nanostructures against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), metal chelating, alpha-amylase and alpha-glucosidase was determined using standard methods. Results: DaZnONPs were observed to be stable, mostly cubical in shape and within the nanometre size range. Optimum absorption of DaZnONPs was observed at 386 nm. The FTIR analysis indicated the presence of functional groups arising from alkaloids, flavonoids, tannins, and saponins (detected in earlier reports) and indicate potential nucleation and stability of the ZnONPs. XRD result depicted similar patterns of DaZnONPs and standard ZnO spectra, revealing a hexagonal and crystalline nature of the particles in nanometre range as shown by the obtained peaks. DaZnONPs inhibited DPPH (0.54 μgmL-1 and alpha-amylase (104.34 μgmL-1 better than quercetin (349.98 μgmL-1 and acarbose (594. 54 μgmL-1. Meanwhile, the metal chelating effect of DaZnONPs (30.41 μgmL-1 was observed to be insignificantly (p>0.05) at par with quercetin (27.81 μgmL-1. The kinetics of alpha-amylase and alpha-glucosidase enzymes by DaZnOnPs was observed to be non-competitive inhibitions. Conclusion: DaZnONPs (as against the bulk extract) could be explored as possible antioxidative and antihyperglycaemic agents mitigating the adverse effects of free radicals and hyperglycaemia.

scholarly journals Potentials of synthesised Lessertia montana zinc oxide nanoparticles on free radicals-mediated oxidative stress and carbohydrate-hydrolysing enzymes

2021 ◽  
Vol 64 (2) ◽  
pp. 239-249
Author(s):  
Fatai O. Balogun ◽  
Anofi O.T. Ashafa
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Free Radicals ◽  
Zinc Oxide Nanoparticles ◽  
Alpha Amylase ◽  
Oxide Nanoparticles ◽  
Zinc Oxide Nanostructures ◽  
Metal Chelating ◽  
Alpha Glucosidase

The study evaluated the effects of green absorbed zinc oxide nanostructures on oxidative stress-mediated free radicals and carbohydrate-hydrolysing enzymes. The synthesised Lessertia montana zinc oxide nanoparticles were characterised using different spectroscopic, microscopic, and diffraction techniques. The activity of L. montana ZnONPs against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid (ABTS), metal chelating assay, alpha-amylase and alphaglucosidase were determined using standard methods. L. montana ZnONPs were stable nanoparticles (NPs), appeared cubical (predominantly) in shape, and in nanometre range sizes. The synthesised NPs are very active (p < 0.05) against DPPH and alpha-glucosidase (0.120 and 0.037 g/L, respectively) when compared with other samples and controls, quercetin (0.349 g/L) and acarbose (0.065 g/L). However, their interaction with quercetin revealed a good ABTS (0.093 g/L) scavenging and an excellent metal chelating (0.027 g/L) effect compared to other samples. The mode of inhibition of alpha-amylase and alpha-glucosidase enzymes by L. montana ZnONPs was competitive and non-competitive, respectively. The study outcomes revealed that the synthesised ZnONPs possessed the potential to mitigate oxidative stress and diabetes in vitro.

scholarly journals Free Radical Scavenging Activity of Fruit Formulation Mediated Zinc Oxide Nanoparticles

2020 ◽  
Vol 11 (3) ◽  
pp. 4690-4694
Author(s):  
Tahoora Taskeen. L ◽  
Hannah R ◽  
Rajeshkumar S
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Free Radicals ◽  
Zinc Oxide Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Free Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Scavenging Activity

To determine the free radical scavenging activity of fruit formulation mediated zinc oxide nanoparticle. Zinc oxide nanoparticles are widely known for their antimicrobial agent, anti-diabetic, anti-inflammatory, wound healing, antioxidant and optic properties. Oxidative degradation of lipids, proteins, food and cosmetics involves a radical-chain reaction with release of free radicals. These free radicals are extremely reactive and can even attack relatively stable molecules like the DNA bases. With a recent rise in diseases associated with increased oxidative stress, a good antioxidant seems to be the need of the hour. Synthesis of zinc oxide nanoparticles using Punica granatum and Elettaria cardamomum extract. Characterisation of the nanoparticles using UV–Visible spectroscopy and Scanning electron microscope. It is followed by the DPPH radical scavenging assay to determine the antioxidant activity. With an increase in the concentration of zinc oxide nanoparticles, there was an increase in the percentage of inhibition. When compared to the standard, the zinc oxide nanoparticles showed increased antioxidant activity even in small concentrations. Punica granatum and Elettaria cardamomum mediated Zinc oxide nanoparticles showed considerable antioxidant property even in small quantity when compared with standard vitamin C. Further studies need to be carried out to check for its potency as a prophylactic agent.

scholarly journals Bioactivities of Geranium wallichianum Leaf Extracts Conjugated with Zinc Oxide Nanoparticles

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 38 ◽  
Author(s):  
Banzeer Ahsan Abbasi ◽  
Javed Iqbal ◽  
Riaz Ahmad ◽  
Layiq Zia ◽  
Sobia Kanwal ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Energy Dispersive ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
X Ray ◽  
Transmission Electron

This study attempts to obtain and test the bioactivities of leaf extracts from a medicinal plant, Geranium wallichianum (GW), when conjugated with zinc oxide nanoparticles (ZnONPs). The integrity of leaf extract-conjugated ZnONPs (GW-ZnONPs) was confirmed using various techniques, including Ultraviolet–visible spectroscopy, X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, energy-dispersive spectra (EDS), scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The size of ZnONPs was approximately 18 nm, which was determined by TEM analysis. Additionally, the energy-dispersive spectra (EDS) revealed that NPs have zinc in its pure form. Bioactivities of GW-ZnONPs including antimicrobial potentials, cytotoxicity, antioxidative capacities, inhibition potentials against α-amylase, and protein kinases, as well as biocompatibility were intensively tested and confirmed. Altogether, the results revealed that GW-ZnONPs are non-toxic, biocompatible, and have considerable potential in biological applications.

Zinc oxide nanoparticles as novel alpha-amylase inhibitors

2008 ◽  
Vol 104 (9) ◽  
pp. 094907 ◽  
Author(s):  
Sandip Dhobale ◽  
Trupti Thite ◽  
S. L. Laware ◽  
C. V. Rode ◽  
Soumya J. Koppikar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Alpha Amylase ◽  
Oxide Nanoparticles ◽  
Amylase Inhibitors

LASER-INDUCED PHOTOLUMINESCENT STUDIES OF Al-DOPED ZINC OXIDE NANOPARTICLES

2010 ◽  
Vol 09 (05) ◽  
pp. 439-445
Author(s):  
DHIRAJ KUMAR ◽  
SUNIL KUMAR ◽  
H. S. BHATTI
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Nitrogen Laser ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
Time Resolved ◽  
X Ray ◽  
Aluminum Ions ◽  
Transmission Electron

In this paper, addition of aluminum in zinc oxide is incorporated using low-temperature chemical synthesis route. Aluminum ions help in crystallization of zinc oxide nanoparticles. Characterization of the synthesized nanoparticles of zinc oxide has been done using Transmission electron microscope (TEM), and X-ray diffraction (XRD) analysis, Energy-resolved photoluminescence (PL) spectra and Time-resolved laser-induced photoluminescence (TRPL) at room temperature. Transmission electron microscopic observations and X-Ray diffraction studies indicate highly crystalline nature and particle size of the order of 20 nm in ZnO:Al . Time-resolved laser-induced photoluminescence measurements have been done using pulsed nitrogen laser as an excitation source, operated at wavelength 337.1 nm and having high peak output power of 1 MW. The results show that at higher concentrations of Al doping in host ZnO phosphor, emission intensity is more by several orders of magnitude and lifetime shortening indicates that these nanoparticles are more efficient as compared with lower concentrations of dopant.

Preparation of Template Free Zinc Oxide Nanoparticles Using Sol–Gel Chemistry

2008 ◽  
Vol 8 (8) ◽  
pp. 4224-4226 ◽  
Author(s):  
Mohan Raja ◽  
A. M. Shanmugaraj ◽  
Sung Hun Ryu
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Sodium Hydroxide Solution ◽  
Sol Gel ◽  
Oxide Nanoparticles ◽  
Zno Nanoparticle ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Controlled Morphology ◽  
Template Free

Zinc oxide (ZnO) nanoparticle has been synthesized via sol–gel chemistry using zinc acetate as a precursor with sodium hydroxide solution through microemulsion technique. X-ray diffraction (XRD) and transmission electron microscopy (TEM) studies reveal that the resultant zinc oxide nanoparticles are wurtzite type with the controlled morphology of nano triangular and half ellipsoid structures. Room temperature photoluminescence measurement of zinc oxide nanoparticles exhibits a strong green band at around 375 nm with excitation energy of 3.30 eV indicating oxygen vacancies on the surface of the nanoparticles.

scholarly journals Laboratory and Commercial Synthesized Zinc Oxide Nanoparticles Adsorption onto Coconut Husk: Characterization, Isotherm, Kinetic, and Thermodynamic Studies

2020 ◽  
Vol 11 (1) ◽  
pp. 7871-7889
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Batch Adsorption ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Maximum Capacity ◽  
Maximum Percentage ◽  
Coconut Husk ◽  
Transmission Electron ◽  
Percentage Removal

The accelerating application of zinc oxide nanoparticles (ZnO-NPs) has called for attention to their potential environmental and human health risks. This work aimed to investigate the sorption efficiency of laboratory and commercial synthesized nanocrystalline zinc oxide onto raw coconut husk in a batch adsorption study. Characterization of samples was performed by employing spectroscopies techniques such as X-ray Diffraction Spectroscopy, Field Emission Scanning Electron Microscopy, Transmission Electron Spectroscopy, Fourier transform IR Spectroscopy, and Brunauer–Emmett–Teller. A spherical shaped nanocrystalline ZnO with a mean crystallite and particle size of 14.7 nm and 24 nm by XRD and TEM was synthesized as compared to the commercial ZnO-NPs of size < 50 nm. The maximum percentage removal of 88% (0.13 mg/g) and 90% (0.16 mg/g) for laboratory synthesized and commercial ZnO-NPs respectively was recorded at an optimum contact time of 80 minutes. The data also indicated 2.0 g sorbent mass and pH of 8 as the optimum conditions for maximum percentage removal of these nanoparticles. Both Langmuir and Freundlich models fitted best for laboratory synthesized ZnO-NPs with a maximum capacity of 0.797 mg/g, whereas Langmuir isotherm model alone with a maximum capacity of 0.710 mg/g fitted well for commercial ZnO-NPs. The n-value from the Freundlich model, as well as separation factor (RL) were greater than unity suggesting a favorable adsorption process. The study obeyed pseudo-second-order, which was exothermic with a high degree of freedom of sorbent-sorbate interaction. The results suggested that coconut husk is potentially scalable for removing ZnO-NPs from wastewater.

scholarly journals Cytotoxic and Antioxidant Activity of Zinc Oxide Nanoparticles Synthesised Using Maranta Arundinacea Root Extract

2020 ◽  
Vol 11 (3) ◽  
pp. 4372-4377
Author(s):  
Aparna J ◽  
Rajeshkumar S
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Large Scale ◽  
Oxide Nanoparticles ◽  
Root Extract ◽  
Scale Production ◽  
Synthesis Of Nanoparticles ◽  
Large Scale Production ◽  
Uv Visible

The main aim of this study was to employ Maranta arundinacea root extract in the synthesis of Zinc oxide nanoparticles and check the antioxidant activity and cytotoxic effect. The use of nanotechnology in medicine especially for drug delivery is shown to have numerous benefits. Nanoparticles are being used to lessen toxicity and facet results that capsules may additionally impose on the patient. The zinc oxide nanoparticles synthesized using different plant extract used for many biomedical applications such as anticancer activity, antibacterial activity, antioxidant activity, antifungal activity and many more applications used in medicine ( Diagnosis and drug) Plant mediated organic synthesis of nanoparticles has been gaining importance because of its simplicity and eco friendliness. Maranta arundinacea mediated Zinc oxide nanoparticles were initially synthesised and characterised by UV-Visible spectrophotometer and TEM. Further the Maranta arundinacea mediated ZnoNPs were subjected to DPPH assay to determine the antioxidant activity. Maranta arundinacea mediated ZnoNPs were biosynthesised with ease and showed good antioxidant and cytotoxic activity . This study concludes that Maranta arundinacea mediated ZnoNPs nanoparticles have the potential to be used as an effective antioxidant. Hence, it may be employed in large scale production and may be used in many medicinal applications where there is a need for antioxidant.

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