Perichondrial Progenitor Cells in Cartilage Tissue Engineering and Regenerative Medicine

2015 ◽  
Vol 4 (2) ◽  
pp. 122-127
Author(s):  
Victor Lin ◽  
Yu-Chieh Wang ◽  
Deborah Watson
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Progenitor Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

Tissue specific stem/progenitor cells for cartilage tissue engineering: A systematic review of the literature

2019 ◽  
Vol 6 (3) ◽  
pp. 031301 ◽  
Author(s):  
Zita M. Jessop ◽  
Susruta Manivannan ◽  
Yadan Zhang ◽  
Catherine A. Thornton ◽  
Roger Narayan ◽  
...  
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Progenitor Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Review Of The Literature ◽  
Tissue Specific

Hypoxic ADSCs-derived EVs Promote the Proliferation and Chondrogenic Differentiation of Cartilage Stem/progenitor Cells

2020 ◽  
Author(s):  
ke xue ◽  
Yongkang Jiang ◽  
Xiaodie Zhang ◽  
Jun Wu ◽  
Lin Qi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Progenitor Cells ◽  
Extracellular Vesicles ◽  
Chondrogenic Differentiation ◽  
Cartilage Tissue Engineering ◽  
Biomechanical Analysis ◽  
Cartilage Tissue ◽  
Alginate Hydrogel ◽  
Cell Based Therapy

Abstract Background： Cartilage tissue engineering is a promising option for repairing cartilage defects caused by trauma, inflammation and osteoarthritis, although harvesting a large number of seeding cells with stable phenotypes remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles secreted by mesenchymal stem cells may play a major role in cell-cell and tissue-tissue communication by transporting various RNAs and proteins in mesenchymal stem cell-based therapy. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived extracellular vesicles (EVs) on CSPCs proliferation and differentiation. Methods: The characteristics of ADSCs-derived EVs were identified by and flow cytometric analysis. Proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. The effect of ADSC-derived EVs on CSPCs were evaluated in alginate hydrogel culture, and SEM, histological staining, biochemical and biomechanical analysis were performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. Results: The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40–1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induced CSPCs to produce significantly more cartilage matrix and proteoglycan. Conclusions: The present study indicated that hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering.

Marine origin materials on biomaterials and advanced therapies to cartilage tissue engineering and regenerative medicine

2021 ◽  
Author(s):  
Duarte Nuno Carvalho ◽  
Rui Reis ◽  
T. H. Silva
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Regenerative Medicine ◽  
Cartilage Tissue Engineering ◽  
The Body ◽  
Cartilage Tissue ◽  
Repair Capacity ◽  
Marine Origin ◽  
The Past ◽  
Advanced Therapies

The body´s self-repair capacity is limited, including injuries on articular cartilage zones. Over the past few decades, tissue engineering and regenerative medicine (TERM) have focused the studies on the development...

scholarly journals Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 348
Author(s):  
Monika Wasyłeczko ◽  
Wioleta Sikorska ◽  
Andrzej Chwojnowski
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Hybrid Materials ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
General Information ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Natural Polymers ◽  
Cellular Environment

Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

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