Caffeic Acid Phenethyl Ester (CAPE), Active Phenolic Compound of Propolis Attenuates Endothelin, Prostaglandin F2α and U46619 Elicited Contractions of Isolated Human Umbilical Artery

2020 ◽  
Vol 06 ◽  
Author(s):  
Ipek Duman ◽  
Burak Cem Soner ◽  
Salim Yalcin Inan ◽  
Ayse Saide Sahin
Keyword(s):  
Caffeic Acid ◽  
Umbilical Artery ◽  
Prostaglandin F2α ◽  
In Vitro Study ◽  
Study Groups ◽  
Caffeic Acid Phenethyl Ester ◽  
Isometric Tension ◽  
Buffer Solution ◽  
Human Umbilical Artery

Background: Propolis is a product of honeybees that contains a variety of different compounds, including caffeic acid phenethyl ester (CAPE). Propolis and its bioactive compounds are widely used in folk medicine and as a dietary supplement. Previously it has been shown that CAPE has antioxidant, anti-inflammatory, antimicrobial, antiviral, immunomodulatory, and anticancer activity. Objective: This in vitro study was designed to investigate the vasoactive effects of CAPE on quiescent and precontracted human umbilical arteries. Methods: Umbilical artery strips were suspended in aerated organ baths containing a buffer solution. The strips were randomly allocated to study groups (n=8). Via a transducer and computer, changes in isometric tension were recorded. The effects of cumulative CAPE (10-8-10-4M) on basal tone of the artery, and in different groups of strips, the vasodilatory effect of cumulative CAPE on the constriction elicited by endothelin (ET-1), prostaglandin F2α (PGF2α) and U46619, and the effect of incubation with NO synthase inhibitor L-NAME, were recorded. Conclusion: CAPE elicits concentration-dependent relaxation on precontracted human umbilical artery strips depending on the constrictor agent. NO plays significant role in CAPE’s vasorelaxant effect.

Histopathologic effects of meconium on human umbilical artery and vein: In vitro study

1997 ◽  
Vol 6 (6) ◽  
pp. 356-361 ◽  
Author(s):  
Ayse Kafkasli ◽  
Michael A. Belfort ◽  
Gregg Giannina ◽  
Yuri P. Vedernikov ◽  
David L. Schaffner ◽  
...  
Keyword(s):  
Umbilical Artery ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Umbilical Artery

In vitro Study of the Effect of Clonidine on Human Umbilical Artery Motility

1994 ◽  
Vol 38 (1) ◽  
pp. 18-20 ◽  
Author(s):  
L.C. França ◽  
S.P. Cunha ◽  
R.M.F. Castro ◽  
N.Y. Nagata
Keyword(s):  
Umbilical Artery ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Umbilical Artery

Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro

2005 ◽  
Vol 521 (1-3) ◽  
pp. 139-143 ◽  
Author(s):  
Francesca Borrelli ◽  
Inmaculada Posadas ◽  
Raffaele Capasso ◽  
Gabriella Aviello ◽  
Valeria Ascione ◽  
...  
Keyword(s):  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester

Formulation development of folic acid conjugated PLGA nanoparticles for improved cytotoxicity of Caffeic acid phenethyl ester

2021 ◽  
Vol 09 ◽  
Author(s):  
Harshad S Kapare ◽  
Sathiyanarayanan L ◽  
Arulmozhi S ◽  
Kakasaheb Mahadik
Keyword(s):  
Folic Acid ◽  
Drug Release ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Therapeutic Effects ◽  
Formulation Development ◽  
Sustained Drug Release ◽  
Active Constituent

Background: Honey bee propolis is one of the natural product reported in various traditional systems of medicines including Ayurveda. Caffeic acid phenethyl ester (CAPE) is an active constituent of propolis which is well known for its anticancer potential. The therapeutic effects of CAPE are restricted owing to its less aqueous solubility and low bioavailability. Objective: In this study CAPE loaded folic acid conjugated nanoparticle system (CLFPN) was investigated to enhance solubility, achieve sustained drug release and improved cytotoxicity of CAPE. Methods: Formulation development, characterization and optimization were carried out by design of experiment approach. In vitro and in vivo cytotoxicity study was carried out for optimized formulations. Results: Developed nanoparticles showed particle size and encapsulation efficiency of 170 ± 2 - 195 ± 3 nm and 75.66 ± 1.52 - 78.80 ± 1.25 % respectively. Optimized formulation CLFPN showed sustained drug release over a period of 42 h. GI50 concentration was decreased by 46.09% for formulation as compared to CAPE in MCF-7 cells indicating targeting effect of CLFPN. An improved in vitro cytotoxic effect was reflected in in-vivo Daltons Ascites Lymphoma model by reducing tumor cells count. Conclusion: The desired nanoparticle characteristic with improved in vivo and in vitro cytotoxicity was shown by developed formulation. Thus it can be further investigated for biomedical applications.

scholarly journals Adhesion of Two New Glass Fiber Post Systems Cemented with Self-Adhesive Resin Cements

2019 ◽  
Vol 7 (3) ◽  
pp. 80 ◽  
Author(s):  
Esin Özlek ◽  
Prasanna Neelakantan ◽  
Jukka Pekka Matinlinna ◽  
Sema Belli ◽  
Mehmet Ugur ◽  
...  
Keyword(s):  
Bond Strength ◽  
Adhesion Strength ◽  
In Vitro Study ◽  
Study Groups ◽  
Fiber Post ◽  
Resin Cements ◽  
Adhesive Resin ◽  
Root Canals ◽  
Group 2

The aim of this in vitro study was to evaluate the adhesion strength of two new fiber post systems (FiberSite™ Post and Cytec™ Blanco Post) cemented with two different adhesive resin cements (Panavia™ SA and Maxcem™ Elite). Root canals of sixty extracted human mandibular premolars were prepared using ProTaper Universal™ rotary files (Dentsply Sirona Endodontics, York, PA, USA). The root canals were irrigated with 5.25% sodium hypochlorite (NaOCl) during instrumentation. After root canal preparation, the canals were irrigated with 2 mL of 17% EDTA (1 min), followed by 2 mL of 5.25% (5 min) NaOCI, and 2 mL saline. The root canals were dried with paper points and divided randomly into two study groups (n = 30) according to the type of post system: Group 1, FiberSite™ Post (MegaDental, Partanna, Italy); and group 2, Cytec™ Blanco Post (Hahnenkratt, Königsbach-Stein, Germany), with one of the two adhesive resin cements: Subgroup A, Panavia™ SA Cement Plus Automix (Kuraray, Osaka, Japan); subgroup B, Maxcem™ Elite (Kerr, Orange, CA, USA). Following thermocycling, the adhesion strength was evaluated using the push-out adhesion (bond) strength test. Fractographic analysis was performed using stereomicroscope. The data were analyzed using two-way analysis of variance (p = 0.05). The adhesion strength values of both the posts were significantly higher when cemented with subgroup B (Maxcem™ Elite). The highest adhesion strength value was demonstrated by group 1B (FiberSite™ post cemented with Maxcem™ Elite cement). The type of post did not have a significant impact on the bond strength values for either cement material.

In Vivo and In Vitro Antıneoplastic Actions of Caffeic Acid Phenethyl Ester (CAPE): Therapeutic Perspectives

2013 ◽  
Vol 65 (4) ◽  
pp. 515-526 ◽  
Author(s):  
Sumeyya Akyol ◽  
Gulfer Ozturk ◽  
Zeynep Ginis ◽  
Ferah Armutcu ◽  
M. Ramazan Yigitoglu ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester

Responses of nerve-free vessels to vasoactive amines and polypeptides

1965 ◽  
Vol 208 (4) ◽  
pp. 748-753 ◽  
Author(s):  
Avril V. Somlyo ◽  
Chi-Yuan Woo ◽  
Andrew P. Somlyo
Keyword(s):  
Umbilical Artery ◽  
Main Pulmonary Artery ◽  
Umbilical Vessels ◽  
Vasoconstrictor Response ◽  
Human Umbilical Artery ◽  
Vasoconstrictor Effect ◽  
Rhythmic Contractions ◽  
Synthetic Oxytocin ◽  
Indirect Action

Contractile responses of helically cut strips of noninnervated human umbilical artery and vein were determined. Spontaneous, rhythmic contractions were exhibited by both preparations, but were greater in magnitude and duration in umbilical veins. Vasoconstriction elicited by sympathomimetic amines was variable, and generally of a low order. The response to norepinephrine was not potentiated by cocaine (10 µg/ml) but was blocked by Dibenamine (1.0–1.5 µg/ml). Umbilical vasoconstrictor response to tyramine (1.0–10.0 µg/ml) indicated the direct vasoconstrictor effect of this agent. The similar norepinephrine-to-tyramine sensitivity ratios of umbilical vessels and canine main pulmonary artery were interpreted as evidence against the indirect action of tyramine in vitro. Isoproterenol produced no vasodilation in umbilical vessels, suggesting the absence of ß-adrenergic pathways. Oxytocin (>>0.1 mU/ml) was a highly effective umbilical vasoconstrictor. Native and synthetic oxytocin preparations were equiactive and produced tachyphylaxis to each other. Native and synthetic lysine-8-vasopressin (>>0.005 U/ml) and angiotensin amide (>>0.002 µg/ml) produced only minimal and inconsistent vasoconstriction, while serotonin (>>0.004 µg/ml) was as effective as oxytocin.

scholarly journals BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study

2020 ◽  
Vol 10 (14) ◽  
pp. 5008
Author(s):  
Deepti Shrivastava ◽  
Kumar Chandan Srivastava ◽  
Jithendra Kajoor Dayakara ◽  
Mohammed G. Sghaireen ◽  
Ravi Kumar Gudipaneni ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
In Vitro Study ◽  
Study Groups ◽  
Vitro Study ◽  
Polymorphonuclear Neutrophils ◽  
H2o2 Production ◽  
Areca Nut

Arecanutchewing is an established risk factor for oral submucous fibrosis (OSMF), but its role in periodontal disease has not yet been defined. Thisstudy aimed to assess the effect of areca nut extracts (ANE) on the bactericidal activity of crevicular polymorphonuclear neutrophils (cPMNs) in healthy subjects and chronic periodontitis (CP) patients. An in vitro study was designed with an equal number of (n = 30) gingival crevicular fluid (GCF) samples collected from CP patients and healthy subjects. Bactericidal activity and hydrogen peroxide (H2O2) assays were performed with the GCF samples pre-treated with extracts of two varieties of areca nut: ripe and tender. Simultaneously, controls were also carried out with Hank’s balanced salt solution (HBSS) and catechin. Independent t-test and one-way analysis of variance (ANOVA), along with post-hoc analysis, were employed for statistical analysis. In both study groups, a significant reduction (p < 0.01)in the bactericidal activity was noted when the samples treated with the ripe areca nut (rANE) were compared with the tender variant (tANE). Similarly, H2O2 levels were significantly reduced (p < 0.001) in the rANE in contrast to tANE for both study groups. The above results were significant within the group but were found to be non-significant between the study groups, except when it was treated with HBSS (p < 0.001). In the present study, it was found that there was a reduction in the bactericidal activity and H2O2 production of cPMNs in both healthy subjects and CP patients in the presence of areca nut extract. Moreover, the effect of rANE on cPMNs was more detrimental than tANE.

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