in vitro antioxidant and Antihypertensive Properties of Edible Cricket (Brachytrupes Membranaceus) Protein Derived Membrane Peptide Fractions

The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

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Antihypertensive and Antioxidant Activity of Chia Protein Techno-Functional Extensive Hydrolysates

Foods ◽

10.3390/foods10102297 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2297

Author(s):

Alvaro Villanueva-Lazo ◽

Sergio Montserrat-de la Paz ◽

Noelia Maria Rodriguez-Martin ◽

Francisco Millan ◽

Cecilio Carrera ◽

...

Keyword(s):

Food Industry ◽

Antioxidant Properties ◽

Protein Isolate ◽

Absorption Capacity ◽

Intact Protein ◽

Physicochemical Composition ◽

Hydrolysis Degree ◽

Antihypertensive Properties ◽

Better Than

Twelve high-quality chia protein hydrolysates (CPHs) were produced from chia protein isolate (CPI) in a pilot plant of vegetable proteins. To obtain functional hydrolysate, four CPHs were hydrolyzed by the action of Alcalase, an endoprotease, and the other eight CPHs were hydrolyzed by the action of Flavourzyme, an exoprotease. Alcalase-obtained CPHs showed significant antihypertensive properties particularly, the CPH obtained after 15 min of hydrolysis with Alcalase (CPH15A), which showed a 36.2% hydrolysis degree. In addition, CPH15A increased the antioxidant capacity compared to CPI. The CPH15A physicochemical composition was characterized and compared to chia defatted flour (CDF) and CPI, and its techno-functional properties were determined by in vitro experiments through the analysis of its oil absorption capacity, as well as the capacity and stability of foaming and emulsifying, resulting in an emulsifier and stabilizer better than the intact protein. Therefore, the present study revealed that CPH15A has potent antihypertensive and antioxidant properties and can constitute an effective alternative to other plant protein ingredients sources that are being used in the food industry.

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Antioxidant and antihypertensive activities of wonderful cola (Buchholzia coriacea) seed protein and enzymatic protein hydrolysates

Journal of Food Bioactives ◽

10.31665/jfb.2018.3156 ◽

2018 ◽

Vol 3 ◽

pp. 133-143 ◽

Cited By ~ 5

Author(s):

Oluwole S. Ijarotimi ◽

Sunday A. Malomo ◽

Adeola M. Alashi ◽

Ifeanyi D. Nwachukwu ◽

Tayo N. Fagbemi ◽

...

Keyword(s):

Blood Pressure ◽

Protein Hydrolysate ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Protein Isolate ◽

Active Inhibitor ◽

Spontaneously Hypertensive ◽

Reducing Ability ◽

Peptide Fractions

The aim of this work was to produce wonderful cola protein hydrolysate fractions with in vitro antioxidant properties coupled with blood pressure-reducing ability when orally administered to spontaneously hypertensive rats (SHRs). Wonderful cola protein isolate (WCI) was hydrolyzed with pancreatin to produce a hydrolysate (WCH), which was subjected to ultrafiltration separation using 1, 3, 5, and 10 kDa molecular weight cut-off membranes to obtain <1, 1–3, 3–5 and 5–10 kDa peptide fractions, respectively. The <1 and 1–3 kDa fractions had higher contents of arginine when compared to the 3–5 and 5–10 kDa peptides. The WCH and <1 kDa peptide fraction had significantly (p < 0.05) better DPPH radical scavenging (55–67%) and metal chelation (83–93%) activities but lower hydroxyl radical scavenging power (10–32%) than the WCI (46, 46 and 63%, respectively). The <1 kDa had significantly (p < 0.05) higher in vitro inhibition (80%) of angiotensin converting enzyme (ACE) activity while the 5–10 kDa was the most active inhibitor (90%) of renin activity. All peptide fractions so produced had better systolic and diastolic blood pressure-lowering effects than WCH and WCI. However, the <1 kDa fraction produced significantly (p < 0.05) stronger systolic (−33 mmHg) and diastolic (−30 mmHg) blood pressure reductions 6 h after oral gavage to SHRs. Thus, wonderful cola proteins contain encrypted bioactive peptides that may be used to formulate antioxidant and antihypertensive products.

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Antioxidant, Antidiabetic, and Antihypertensive Properties of Echinacea purpurea Flower Extract and Caffeic Acid Derivatives Using In Vitro Models

Journal of Medicinal Food ◽

10.1089/jmf.2016.3790 ◽

2017 ◽

Vol 20 (2) ◽

pp. 171-179 ◽

Cited By ~ 25

Author(s):

Shiow-Ying Chiou ◽

Jih-Min Sung ◽

Po-Wei Huang ◽

Sheng-Dun Lin

Keyword(s):

Caffeic Acid ◽

Echinacea Purpurea ◽

In Vitro Models ◽

Caffeic Acid Derivatives ◽

Flower Extract ◽

Antihypertensive Properties

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Demonstration of in vitro anticancer properties of peptide fractions from a snow crab by-products hydrolysate after separation by electrodialysis with ultrafiltration membranes

Separation and Purification Technology ◽

10.1016/j.seppur.2011.01.037 ◽

2011 ◽

Vol 78 (3) ◽

pp. 321-329 ◽

Cited By ~ 46

Author(s):

Alain Doyen ◽

Lucie Beaulieu ◽

Linda Saucier ◽

Yves Pouliot ◽

Laurent Bazinet

Keyword(s):

Snow Crab ◽

Ultrafiltration Membranes ◽

Anticancer Properties ◽

Peptide Fractions ◽

By Products

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EFFECTS OF CASTRATION, AND TESTOSTERONE IN VITRO, ON THE HYPOTHALAMIC SYNTHESIS OF DIFFERENT PEPTIDE FRACTIONS

Journal of Endocrinology ◽

10.1677/joe.0.0640155 ◽

1975 ◽

Vol 64 (1) ◽

pp. 155-161 ◽

Cited By ~ 2

Author(s):

J. A. MOGUILEVSKY ◽

M. A. ENERO ◽

B. SZWARCFARB ◽

D. DOSORETZ

Keyword(s):

Luteinizing Hormone ◽

Incubation Medium ◽

Ammonium Acetate ◽

Follicle Stimulating Hormone ◽

The Other ◽

Ovariectomized Rats ◽

Small Peptides ◽

Other Hand ◽

Peptide Fractions

SUMMARY The incorporation of [3H]tyrosine ([3H]tyr) into different hypothalamic peptide fractions isolated from normal and castrated rats on a Sephadex G-25 column has been studied in vitro. Luteinizing hormone releasing factor (LH-RF) activity was determined in the different fractions by measuring their ability to elicit release of radioimmunoassayable luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in ovariectomized rats treated with oestrogen and progesterone. For further purification, the fraction with LH-RF activity was applied to a CM-Sephadex G-25 column eluted with a gradient of ammonium acetate. The large radioactive peptides emerged from the Sephadex G-25 column in fraction S-1, while the small peptides with LH-RF activity were eluted in fraction S-2. Gonadectomy significantly increased the incorporation of [3H]tyr into the peptides of fractions S-2. Only in the purified fraction with LH-RF activity was the radioactivity incorporated higher in gonadectomized than in normal rats. The enhanced incorporation in fraction CM-3 observed after castration implies an increase in the hypothalamic synthesis of peptides with LH-RF activity. The addition of testosterone (2 μg/ml) to the incubation medium of hypothalamus from gonadectomized rats, corrected these modifications. Gonadectomy decreased the incorporation of tyrosine into the large peptides, and incubation with testosterone corrected this change. The modifications in the incorporation of [3H]tyr into the large and small peptides produced by castration appear to indicate that gonadectomy, as well as stimulating the production of LH-RF, enhances the synthesis of other hypothalamic peptides while inhibiting the synthesis of proteins. On the other hand an increase in the breakdown of large peptides into small peptides cannot be excluded.

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