scholarly journals Optimize Highly Efficient Genetic Transformation Method of Spring Wheat (Triticum aestivum L.) Cultivars Using Mature Embryo

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (22) ◽  
Author(s):  
Dongjin Kim ◽  
Megan Hager
Keyword(s):  
Triticum Aestivum ◽  
Genetic Transformation ◽  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Transformation Method ◽  
Mature Embryo ◽  
Highly Efficient ◽  
Efficient Genetic Transformation

scholarly journals Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.)

2018 ◽  
Vol 22 ◽  
pp. 293-298
Author(s):  
S. I. Mykhalska ◽  
A. G. Komisarenko ◽  
V. M. Kurchii ◽  
O. M. Tishchenko
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Winter Wheat ◽  
Natural Frequency ◽  
Triticum Aestivum L ◽  
Transformation Method ◽  
In Planta ◽  
Agrobacterium Mediated Transformation ◽  
Pollination Methods

Aim. To optimize the agrobacterium-mediated method of winter wheat transformation (Triticum aestivum L.); to select the conditions and period of inoculation to effectively transfer the genes during pollination. Methods. Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.) during pollination. Results. The conditions for agrobacterium-mediated transformation method of winter wheat during natural (frequency pollination was 1 %) and non-natural (frequency pollination was 4 %) pollination were defined. Conclusions. The possibility of integrating transgenes into the genome of winter wheat plants by the method of Agrobacterium-mediated transformation in planta in the process of forced and natural pollination is demonstrated. It is found that the transformation efficiency to a large extent depends on the plant genotype and the method of carrying out the transformation procedure. The selection of transgenic plants under water deficit conditions allowed to identify the plants with functional transgene. The signs of functioning transgene have been remaining in the next generation of genetically modified winter wheat. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation in planta, transgenic plants, seeds.

scholarly journals Mapping quantitative trait loci associated with common bunt resistance in a spring wheat (Triticum aestivum L.) variety Lillian

2019 ◽  
Vol 132 (11) ◽  
pp. 3023-3033 ◽  
Author(s):  
Firdissa E. Bokore ◽  
Richard D. Cuthbert ◽  
Ron E. Knox ◽  
Arti Singh ◽  
Heather L. Campbell ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Quantitative Trait Loci ◽  
Spring Wheat ◽  
Quantitative Trait ◽  
Triticum Aestivum L ◽  
Common Bunt ◽  
Trait Loci ◽  
Bunt Resistance

Biggar red spring wheat

1991 ◽  
Vol 71 (2) ◽  
pp. 519-522 ◽  
Author(s):  
R. M. DePauw ◽  
K. R. Preston ◽  
T. F. Townley-Smith ◽  
E. A. Hurd ◽  
G. E. McCrystal ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Yield Potential ◽  
High Yield ◽  
Flour Milling ◽  
Wide Adaptation ◽  
Cultivar Description ◽  
End Use ◽  
Milling Properties

Biggar red spring wheat (Triticum aestivum L.) combines high grain yield potential with semidwarf stature and wide adaptation. Biggar has improved end-use suitability relative to HY320 such as harder kernels, better flour milling properties, greater water absorption, and stronger gluten properties. It received registration No. 3089 and is eligible for grades of Canada Prairie Spring (red). Key words: Triticum aestivum, wheat (spring), high yield, cultivar description

EFFECTS OF INCREMENTAL N FERTILIZATION ON GRAIN YIELD AND DRY MATTER ACCUMULATION OF SIX SPRING WHEAT (Triticum aestivum L.) CULTIVARS IN SOUTHERN MANITOBA

1990 ◽  
Vol 70 (1) ◽  
pp. 51-60 ◽  
Author(s):  
D. T. GEHL ◽  
L. D. BAILEY ◽  
C. A. GRANT ◽  
J. M. SADLER
Keyword(s):  
Triticum Aestivum ◽  
Grain Yield ◽  
Spring Wheat ◽  
Dry Matter ◽  
Root Zone ◽  
Temporal Distribution ◽  
Triticum Aestivum L ◽  
N Fertilization ◽  
Yield Response ◽  
Dry Matter Accumulation

A 3-yr study was conducted on three Orthic Black Chernozemic soils to determine the effects of incremental N fertilization on grain yield and dry matter accumulation and distribution of six spring wheat (Triticum aestivum L.) cultivars. Urea (46–0–0) was sidebanded at seeding in 40 kg N ha−1 increments from 0 to 240 kg ha−1 in the first year and from 0 to 200 kg ha−1 in the 2 subsequent years. Nitrogen fertilization increased the grain and straw yields of all cultivars in each experiment. The predominant factor affecting the N response and harvest index of each cultivar was available moisture. At two of the three sites, 91% of the interexperiment variability in mean maximum grain yield was explained by variation in root zone moisture at seeding. Mean maximum total dry matter varied by less than 12% among cultivars, but mean maximum grain yield varied by more than 30%. Three semidwarf cultivars, HY 320, Marshall and Solar, had consistently higher grain yield and grain yield response to N than Glenlea and Katepwa, two standard height cultivars, and Len, a semidwarf. The mean maximum grain yield of HY 320 was the highest of the cultivars on test and those of Katepwa and Len the lowest. Len produced the least straw and total dry matter. The level of N fertilization at maximum grain yield varied among cultivars, sites and years. Marshall and Solar required the highest and Len the lowest N rates to achieve maximum grain yield. The year-to-year variation in rates of N fertilization needed to produce maximum grain yield on a specific soil type revealed the limitations of N fertility recommendations based on "average" amounts and temporal distribution of available moisture.Key words: Wheat (spring), N response, standard height, semidwarf, grain yield

scholarly journals The genetics of late maturity alpha-amylase (LMA) in North American spring wheat (Triticum aestivum L.)

2021 ◽  
pp. 1-10
Author(s):  
Chang Liu ◽  
Rehana S. Parveen ◽  
Samuel R. Revolinski ◽  
Kimberly A. Garland Campbell ◽  
Michael O. Pumphrey ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Spring Wheat ◽  
North American ◽  
Genome Wide Association Study ◽  
Triticum Aestivum L ◽  
Preharvest Sprouting ◽  
Alpha Amylase ◽  
Reduced Height ◽  
Increased Risk ◽  
Late Maturity

Abstract Genetic susceptibility to late maturity alpha-amylase (LMA) in wheat (Triticum aestivum L.) results in increased alpha-amylase activity in mature grain when cool conditions occur during late grain maturation. Farmers are forced to sell wheat grain with elevated alpha-amylase at a discount because it has an increased risk of poor end-product quality. This problem can result from either LMA or preharvest sprouting, grain germination on the mother plant when rain occurs before harvest. Whereas preharvest sprouting is a well-understood problem, little is known about the risk LMA poses to North American wheat crops. To examine this, LMA susceptibility was characterized in a panel of 251 North American hard spring wheat lines, representing ten geographical areas. It appears that there is substantial LMA susceptibility in North American wheat since only 27% of the lines showed reproducible LMA resistance following cold-induction experiments. A preliminary genome-wide association study detected six significant marker-trait associations. LMA in North American wheat may result from genetic mechanisms similar to those previously observed in Australian and International Maize and Wheat Improvement Center (CIMMYT) germplasm since two of the detected QTLs, QLMA.wsu.7B and QLMA.wsu.6B, co-localized with previously reported loci. The Reduced height (Rht) loci also influenced LMA. Elevated alpha-amylase levels were significantly associated with the presence of both wild-type and tall height, rht-B1a and rht-D1a, loci in both cold-treated and untreated samples.

HARMFUL EFFECT OF WORKED-DOWN WINTER WHEAT ON SPRING-SEEDED WHEAT AND RAPESEED

1983 ◽  
Vol 63 (1) ◽  
pp. 299-301 ◽  
Author(s):  
S. FREYMAN ◽  
G. B. SCHAALJE
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Spring Wheat ◽  
Detrimental Effect ◽  
Brassica Campestris ◽  
Harmful Effect ◽  
Triticum Aestivum L ◽  
Causative Effect ◽  
Effect On Yield

Where winter wheat (Triticum aestivum L. ’Norstar’) was worked-down on 1 May and the plots reseeded to spring wheat immediately, no detrimental effect on yield of spring wheat was found. However, delaying this action until 15 May reduced the yields of spring-seeded wheat because of the harmful effect of decomposing winter wheat and late seeding. Moisture depletion by winter wheat was eliminated as a causative effect by light irrigations during May. Yields of rapeseed (Brassica campestris L. ’Candle’) were not so severely reduced by worked-down winter wheat. The harmful effect was significant only with 30 May cultivation and seeding date.Key words: Phytotoxicity, Triticum aestivum, Brassica campestris, worked-down

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