scholarly journals Cultivation of Malignant Tumor Cells on the Chorioallantoic Membrane of Embryonated Chicken Eggs

1959 ◽  
Vol 35 (8) ◽  
pp. 518-523
Author(s):  
Susumu HOTTA ◽  
Akio OHYAMA ◽  
Hideo AOKI
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Chorioallantoic Membrane ◽  
Chicken Eggs ◽  
Embryonated Chicken

An Epidemiological Study of Sheep and Goat pox Outbreaks in the Sudan

Food Biology ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Ahmed Zein Elabdeen Mahmoud ◽  
Abdelmalik Ibrahim Khalafalla ◽  
Muaz Magzob Abdellatif
Keyword(s):  
Virus Isolation ◽  
Chorioallantoic Membrane ◽  
Vero Cells ◽  
Fibroblast Cells ◽  
Chick Embryo Fibroblast ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Species Specific ◽  
Chicken Eggs ◽  
Embryonated Chicken

Sheep and goat pox Outbreaks occurred in different geographic areas of Sudan and most strikingly, were highly species specific. Two outbreaks in Gedarif State in June. 2013 affected no goats and outbreak in Khartoum state in March. 2015 affected no sheep despite communal herding; affected goats were vaccinated with 0240 strain. Clinically, the disease was characterized by fever, depression and eruption of generalized pox lesions. Mortality rate ranged between 5.2 and 6.7% with a mean of 6.1%. Isolation of viruses succeed on Lamb testes cell culture at passage four, the diseases were diagnosed using virus neutralisation test and polymerase chain reaction. Sheeppox and goatpox isolates grew well in lamb testes and Vero cells. In MDBK however, both viruses induced slight CPE that reached 60% in 9 days. On the other hand, both isolates induced no CPE in chick embryo fibroblast cells. Virus isolation attempts failed on chorioallantoic membrane of embryonated chicken eggs.

scholarly journals Development and Evaluation of a Live Attenuated Egg-Based Camelpox Vaccine

2021 ◽  
Vol 8 ◽  
Author(s):  
Kuandyk Zhugunissov ◽  
Sanat Kilibayev ◽  
Muratbay Mambetaliyev ◽  
Kunsulu Zakarya ◽  
Markhabat Kassenov ◽  
...  
Keyword(s):  
Virus Strain ◽  
Wild Type Strain ◽  
Protective Efficacy ◽  
Chorioallantoic Membrane ◽  
Economic Loss ◽  
Viral Disease ◽  
Wild Type ◽  
Post Vaccination ◽  
Chicken Eggs ◽  
Embryonated Chicken

Camelpox is an infectious viral disease of camels reported in all the camel-breeding areas of Africa, north of the equator, the Middle East and Asia. It causes huge economic loss to the camel industry. We developed a live camelpox virus vaccine candidate using an attenuated strain and evaluated its safety, immunogenicity and protective efficacy in camels. The attenuated virus strain was generated from the camelpox wild-type strain M-96 by 40 consecutive passages on the chorioallantoic membrane of 11-day-old embryonated chicken eggs, henceforth called KM-40 strain. Reversion to virulence of the KM-40 strain was evaluated in camels by three serial passages, confirmed its inability to revert to virulence and its overdose administration was also found safe. Studies of immunogenicity and protective efficacy of the candidate vaccine KM-40 strain in camels was carried out using the dose of 5 x 104.0 EID50. Our data showed complete protection against the challenge infection using the virulent wild-type camelpox virus strain M-96 (dose of 105.0 EID50) which was evaluated at 1, 3, 6 and 12 months post vaccination. In summary, our candidate live attenuated egg-based camelpox vaccine strain KM-40 was found safe, protective, and thus has the potential to use safely in field conditions.

scholarly journals Egg Fluids and Cells of the Chorioallantoic Membrane of Embryonated Chicken Eggs Can Select Different Variants of Influenza A (H3N2) Viruses

Virology ◽  
1995 ◽  
Vol 211 (1) ◽  
pp. 302-306 ◽  
Author(s):  
Charles T. Hardy ◽  
Sarah A. Young ◽  
Robert G. Webster ◽  
Clayton W. Naeve ◽  
Randall J. Owens
Keyword(s):  
Influenza A ◽  
Chorioallantoic Membrane ◽  
Chicken Eggs ◽  
Embryonated Chicken

CYTOLOGICAL AND IMMUNOCYTOCHEMICAL DIAGNOSIS OF TUMOR PLEURITIS

2017 ◽  
Vol 63 (6) ◽  
pp. 894-899
Author(s):  
Viktor Novik ◽  
A. Nefedova ◽  
Ye. Yakubo ◽  
O. Ivanov ◽  
Yekaterina Shalina ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Cytological Study ◽  
False Negative ◽  
Unknown Primary ◽  
Cytological Examination ◽  
Immunocytochemical Studies ◽  
Negative Conclusion ◽  
Primary Localization

Cytological examination of smears from the sediment after centrifugation of pleural fluids was performed in 479 patients who underwent examination and treatment at our institution in 2014-2016. In 249 (52%) patients tumor cells were not detected in smears, in 230 (48%) observations a suspicion (28 observations) or a confident conclusion (202 observations) on the presence of malignant tumor cells in the exudates was cytologically expressed. In 38 cases immunocytochemical studies was additionally performed. In two observations a false-negative conclusion about the absence of tumor cells in smears was expressed. The sensitivity of the cytological study in the diagnosis of malignant pleuritis was 99.0%. Affirmative cytological conclusions on the presence of malignant pleuritis were given in 87.0% of observations, suspicious cytological responses - in 12.0% of cases. Immunocytochemical studies significantly expanded the possibilities of cytological research and were of great importance in the diagnosis of metastases of tumors of unknown primary localization.

Sign in / Sign up

Export Citation Format

Share Document