scholarly journals Antiangiogenesis activity test of tin leaf (Ficus carica L.) on the number of blood vessels and VEGF expression of chorioallantoic membrane of embryonated chicken eggs

2018 ◽  
Vol 1 (4) ◽  
pp. e00007
Author(s):  
Iwan Sahrial Hamid ◽  
Eduardus Bimo Aksono ◽  
Mohammad Sukmanadi ◽  
Muhammad Thohawi Elziyad Purnama
Keyword(s):  
Blood Vessels ◽  
Chorioallantoic Membrane ◽  
Ficus Carica ◽  
Vegf Expression ◽  
Activity Test ◽  
Chicken Eggs ◽  
Embryonated Chicken

scholarly journals Modeling influenza virus infection in mature Wistar rats

2020 ◽  
Vol 65 (3) ◽  
pp. 159-166
Author(s):  
V. A. Marchenko ◽  
S. V. Barashkova ◽  
I. A. Zelinskaya ◽  
Y. G. Toropova ◽  
E. V. Sorokin ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Cardiovascular System ◽  
Blood Vessels ◽  
Wistar Rats ◽  
Pulmonary Vessels ◽  
Influenza Virus A ◽  
Infectious Titer ◽  
Last Stage ◽  
Chicken Eggs ◽  
Embryonated Chicken

It has now been established that blood vessels are target for influenza, but the mechanism by which the influenza virus affects the cardiovascular system is unknown. The aim – adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats, as these animals are the main experimental model for studying the pathology of the cardiovascular system. Material and methods. Passage of influenza A virus (IAV) in embryonated chicken eggs, intranasal inoculation of rats with virus-containing material s, production of pulmonary homogenate, determination of IAV titer in embryonated chicken eggs, detection of histological changes in lung and pulmonary vessels. Results. The article presents the results of the adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats. The infectious titer of the virus in the homogenates of infected rats lungs at the last stage of adaptation was 7.0 lg EID50/ml. Histological studies revealed pronounced changes in the respiratory tract (spasm of bronchioles, submucosal edema, desquamation of ciliated epithelium of bronchioles) and pulmonary vessels (spasm, desquamation and swelling of endotheliocytes, dissociation and swelling of the elastic membrane and media). In order to identify IAV in blood vessels and lung tissues, an immunohistochemical study was performed using monoclonal antibodies to NP antigen of IAV. Conclusion. The data obtained allow us to conclude that the strain of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) was adapted to mature Wistar rats maintaining virulent properties. The infectious titer of the virus at the last stage of adaptation was 7.0 lg EID50/ml. IAV identification is confirmed by immunohistochemical examination.

An Epidemiological Study of Sheep and Goat pox Outbreaks in the Sudan

Food Biology ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Ahmed Zein Elabdeen Mahmoud ◽  
Abdelmalik Ibrahim Khalafalla ◽  
Muaz Magzob Abdellatif
Keyword(s):  
Virus Isolation ◽  
Chorioallantoic Membrane ◽  
Vero Cells ◽  
Fibroblast Cells ◽  
Chick Embryo Fibroblast ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Species Specific ◽  
Chicken Eggs ◽  
Embryonated Chicken

Sheep and goat pox Outbreaks occurred in different geographic areas of Sudan and most strikingly, were highly species specific. Two outbreaks in Gedarif State in June. 2013 affected no goats and outbreak in Khartoum state in March. 2015 affected no sheep despite communal herding; affected goats were vaccinated with 0240 strain. Clinically, the disease was characterized by fever, depression and eruption of generalized pox lesions. Mortality rate ranged between 5.2 and 6.7% with a mean of 6.1%. Isolation of viruses succeed on Lamb testes cell culture at passage four, the diseases were diagnosed using virus neutralisation test and polymerase chain reaction. Sheeppox and goatpox isolates grew well in lamb testes and Vero cells. In MDBK however, both viruses induced slight CPE that reached 60% in 9 days. On the other hand, both isolates induced no CPE in chick embryo fibroblast cells. Virus isolation attempts failed on chorioallantoic membrane of embryonated chicken eggs.

scholarly journals Evaluation of Anti-angiogenic Activity of Centella asiatica Water Extract on Chick Embryo Chorioallantoic Membrane Induced by bFGF

2013 ◽  
Vol 4 (1) ◽  
pp. 463
Author(s):  
Iwan Sahrial Hamid ◽  
Ngakan Made Rai Widjaja
Keyword(s):  
Growth Factor ◽  
Treatment Group ◽  
Cancer Cells ◽  
Blood Vessels ◽  
Chorioallantoic Membrane ◽  
Water Extract ◽  
Centella Asiatica ◽  
Natural Materials ◽  
Treatment Groups ◽  
Embryonated Chicken

Angiogenesis is a physiological process that involves the formation of new blood vessels. In the cancer growth process, angiogenesis acts by supplying nutrition and oxygen for the cancer cells, carries metabolic waste and biological end product of cancer cells. Therefore the spread of cancer cells to the surrounding cells and tissues is accelerated. The occurrence of angiogenesis shows a change in cancer cell status, from dormant to malignant. Cancer cells induce the formation of angiogenesis through the secretion of various growth factors such as Vascular Endothelial Growth Factor (VEGF) and basic Fibroblast Growth Factor (bFGF).Currently many people turn into treatment using natural materials. One of the natural materials used for cancer therapy is pegagan (Centella asiatica). In this research 25 embryonated chicken eggs (ECE) were divided into five treatment groups. Treatment group I as the control received 60 ng bFGF, treatment group II received neither bFGF nor pegagan leaves (Centella asiatica) water extract, and  treatment groups III-V received 60 ng bFGF and  C. asiatica leaves water extract of 75, 90 and 110 µg.  Nine days old ESE were inoculated with bFGF and extract at the same time and then were incubated at 380C for three days to allow the formation of blood vessels in embryonated chicken egg chorioallantoic membrane.  Observations were performed microscopically and macroscopically by counting the new blood vessels formed around paper disc. Analysis on VEGF expression were conducted to define the role of angiogenesis growth factor.Research results showed a significant difference of treatment with Centella asiatica water extract on the number of new blood vessels formed. Centella asiatica water extract at the dose of 110 µg was the highest in inhibiting the formation of new blood vessels. Centella asiatica water extract also inhibited the expression of VEGF.Keywords : Centella asiatica, antiangiogenic, Corio Allantois Membran, VEGF

scholarly journals Development and Evaluation of a Live Attenuated Egg-Based Camelpox Vaccine

2021 ◽  
Vol 8 ◽  
Author(s):  
Kuandyk Zhugunissov ◽  
Sanat Kilibayev ◽  
Muratbay Mambetaliyev ◽  
Kunsulu Zakarya ◽  
Markhabat Kassenov ◽  
...  
Keyword(s):  
Virus Strain ◽  
Wild Type Strain ◽  
Protective Efficacy ◽  
Chorioallantoic Membrane ◽  
Economic Loss ◽  
Viral Disease ◽  
Wild Type ◽  
Post Vaccination ◽  
Chicken Eggs ◽  
Embryonated Chicken

Camelpox is an infectious viral disease of camels reported in all the camel-breeding areas of Africa, north of the equator, the Middle East and Asia. It causes huge economic loss to the camel industry. We developed a live camelpox virus vaccine candidate using an attenuated strain and evaluated its safety, immunogenicity and protective efficacy in camels. The attenuated virus strain was generated from the camelpox wild-type strain M-96 by 40 consecutive passages on the chorioallantoic membrane of 11-day-old embryonated chicken eggs, henceforth called KM-40 strain. Reversion to virulence of the KM-40 strain was evaluated in camels by three serial passages, confirmed its inability to revert to virulence and its overdose administration was also found safe. Studies of immunogenicity and protective efficacy of the candidate vaccine KM-40 strain in camels was carried out using the dose of 5 x 104.0 EID50. Our data showed complete protection against the challenge infection using the virulent wild-type camelpox virus strain M-96 (dose of 105.0 EID50) which was evaluated at 1, 3, 6 and 12 months post vaccination. In summary, our candidate live attenuated egg-based camelpox vaccine strain KM-40 was found safe, protective, and thus has the potential to use safely in field conditions.

scholarly journals Egg Fluids and Cells of the Chorioallantoic Membrane of Embryonated Chicken Eggs Can Select Different Variants of Influenza A (H3N2) Viruses

Virology ◽  
1995 ◽  
Vol 211 (1) ◽  
pp. 302-306 ◽  
Author(s):  
Charles T. Hardy ◽  
Sarah A. Young ◽  
Robert G. Webster ◽  
Clayton W. Naeve ◽  
Randall J. Owens
Keyword(s):  
Influenza A ◽  
Chorioallantoic Membrane ◽  
Chicken Eggs ◽  
Embryonated Chicken

scholarly journals Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3284
Author(s):  
Muhammad Zahid ◽  
Maria Lodhi ◽  
Zulfiqar Ahmad Rehan ◽  
Hamna Tayyab ◽  
Talha Javed ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Granulation Tissue ◽  
Chorioallantoic Membrane ◽  
Calotropis Procera ◽  
Connective Tissues ◽  
Blood Capillaries ◽  
Chick Chorioallantoic Membrane ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

scholarly journals Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marloes Heijne ◽  
Martina Jelocnik ◽  
Alexander Umanets ◽  
Michael S. M. Brouwer ◽  
Annemieke Dinkla ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Opportunistic Pathogen ◽  
Chlamydia Psittaci ◽  
Phenotypic Analysis ◽  
Pathogenic Potential ◽  
The Family ◽  
Chicken Eggs ◽  
Sequence Types ◽  
Insight Into ◽  
Embryonated Chicken

AbstractChlamydia gallinacea is an obligate intracellular bacterium that has recently been added to the family of Chlamydiaceae. C. gallinacea is genetically diverse, widespread in poultry and a suspected cause of pneumonia in slaughterhouse workers. In poultry, C. gallinacea infections appear asymptomatic, but studies about the pathogenic potential are limited. In this study two novel sequence types of C. gallinacea were isolated from apparently healthy chickens. Both isolates (NL_G47 and NL_F725) were closely related to each other and have at least 99.5% DNA sequence identity to C. gallinacea Type strain 08-1274/3. To gain further insight into the pathogenic potential, infection experiments in embryonated chicken eggs and comparative genomics with Chlamydia psittaci were performed. C. psittaci is a ubiquitous zoonotic pathogen of birds and mammals, and infection in poultry can result in severe systemic illness. In experiments with embryonated chicken eggs, C. gallinacea induced mortality was observed, potentially strain dependent, but lower compared to C. psittaci induced mortality. Comparative analyses confirmed all currently available C. gallinacea genomes possess the hallmark genes coding for known and potential virulence factors as found in C. psittaci albeit to a reduced number of orthologues or paralogs. The presence of potential virulence factors and the observed mortality in embryonated eggs indicates C. gallinacea should rather be considered as an opportunistic pathogen than an innocuous commensal.

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