Gilberto D. Vargas Virucidal activity of green propolis against avipoxvirus in chorioallantoic membrane of embryonated chicken eggs

2011 ◽  
Vol 5 (9) ◽  
Author(s):  
Camila O. Vilela
Keyword(s):  
Chorioallantoic Membrane ◽  
Virucidal Activity ◽  
Chicken Eggs ◽  
Embryonated Chicken

An Epidemiological Study of Sheep and Goat pox Outbreaks in the Sudan

Food Biology ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Ahmed Zein Elabdeen Mahmoud ◽  
Abdelmalik Ibrahim Khalafalla ◽  
Muaz Magzob Abdellatif
Keyword(s):  
Virus Isolation ◽  
Chorioallantoic Membrane ◽  
Vero Cells ◽  
Fibroblast Cells ◽  
Chick Embryo Fibroblast ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Species Specific ◽  
Chicken Eggs ◽  
Embryonated Chicken

Sheep and goat pox Outbreaks occurred in different geographic areas of Sudan and most strikingly, were highly species specific. Two outbreaks in Gedarif State in June. 2013 affected no goats and outbreak in Khartoum state in March. 2015 affected no sheep despite communal herding; affected goats were vaccinated with 0240 strain. Clinically, the disease was characterized by fever, depression and eruption of generalized pox lesions. Mortality rate ranged between 5.2 and 6.7% with a mean of 6.1%. Isolation of viruses succeed on Lamb testes cell culture at passage four, the diseases were diagnosed using virus neutralisation test and polymerase chain reaction. Sheeppox and goatpox isolates grew well in lamb testes and Vero cells. In MDBK however, both viruses induced slight CPE that reached 60% in 9 days. On the other hand, both isolates induced no CPE in chick embryo fibroblast cells. Virus isolation attempts failed on chorioallantoic membrane of embryonated chicken eggs.

scholarly journals Development and Evaluation of a Live Attenuated Egg-Based Camelpox Vaccine

2021 ◽  
Vol 8 ◽  
Author(s):  
Kuandyk Zhugunissov ◽  
Sanat Kilibayev ◽  
Muratbay Mambetaliyev ◽  
Kunsulu Zakarya ◽  
Markhabat Kassenov ◽  
...  
Keyword(s):  
Virus Strain ◽  
Wild Type Strain ◽  
Protective Efficacy ◽  
Chorioallantoic Membrane ◽  
Economic Loss ◽  
Viral Disease ◽  
Wild Type ◽  
Post Vaccination ◽  
Chicken Eggs ◽  
Embryonated Chicken

Camelpox is an infectious viral disease of camels reported in all the camel-breeding areas of Africa, north of the equator, the Middle East and Asia. It causes huge economic loss to the camel industry. We developed a live camelpox virus vaccine candidate using an attenuated strain and evaluated its safety, immunogenicity and protective efficacy in camels. The attenuated virus strain was generated from the camelpox wild-type strain M-96 by 40 consecutive passages on the chorioallantoic membrane of 11-day-old embryonated chicken eggs, henceforth called KM-40 strain. Reversion to virulence of the KM-40 strain was evaluated in camels by three serial passages, confirmed its inability to revert to virulence and its overdose administration was also found safe. Studies of immunogenicity and protective efficacy of the candidate vaccine KM-40 strain in camels was carried out using the dose of 5 x 104.0 EID50. Our data showed complete protection against the challenge infection using the virulent wild-type camelpox virus strain M-96 (dose of 105.0 EID50) which was evaluated at 1, 3, 6 and 12 months post vaccination. In summary, our candidate live attenuated egg-based camelpox vaccine strain KM-40 was found safe, protective, and thus has the potential to use safely in field conditions.

scholarly journals Egg Fluids and Cells of the Chorioallantoic Membrane of Embryonated Chicken Eggs Can Select Different Variants of Influenza A (H3N2) Viruses

Virology ◽  
1995 ◽  
Vol 211 (1) ◽  
pp. 302-306 ◽  
Author(s):  
Charles T. Hardy ◽  
Sarah A. Young ◽  
Robert G. Webster ◽  
Clayton W. Naeve ◽  
Randall J. Owens
Keyword(s):  
Influenza A ◽  
Chorioallantoic Membrane ◽  
Chicken Eggs ◽  
Embryonated Chicken

scholarly journals Simple, Low-cost and Semi-permanent Film for Virus Inactivation Using Avian Coronavirus Model as Challenge

2020 ◽  
Author(s):  
Luiz Alberto Colnago ◽  
Iara Maria Trevisol ◽  
Daiane Voss Rech ◽  
Lucimara Aparecida Forato ◽  
Cirlei Igreja do Nascimento Mitre ◽  
...  
Keyword(s):  
Rna Virus ◽  
Low Cost ◽  
Virus Infectivity ◽  
Virucidal Activity ◽  
Lower Socioeconomic Status ◽  
Lower Socioeconomic ◽  
Dishwasher Detergent ◽  
Chicken Eggs ◽  
Embryonated Chicken ◽  
Virus Quantification

COVID-19 infection, caused by SARS-CoV-2, is inequitably distributed and more lethal among populations with lower socioeconomic status. Direct contact with contaminated surfaces has been one of the virus sources, as it remains infective up to days. Several disinfectants have been shown to inactivate SARS-CoV-2 but they rapidly evaporate, are flammable or toxic and may be scarce or inexistent for the vulnerable populations. Therefore, we are proposing a simple, easy to prepare, low-cost and efficient antiviral films, made with wide available dishwasher detergent, which can be spread in hands and inanimate surfaces and maintains virucidal activity for longer periods than the current sanitizers. Avian coronavirus (ACoV) was used as model of challenge to test the antivirus efficacy of proposed films. Polystyrene microplates were covered with a thin layer of detergent formula. After drying, the films were exposed to different virus doses for 10 minutes and virus infectivity were determined using embryonated chicken eggs and RNA virus quantification in allantoic fluids by RT-qPCR. The films showed to inactive the ACoV (ranging from 103.66 to 106.66 EID50), which is chemically and morphologically similar to SARSCoV-2 and may constitute an excellent alternative to minimize the spread of Covid-19.

scholarly journals Simple, Low-Cost and Long-Lasting Film for Virus Inactivation Using Avian Coronavirus Model as Challenge

2020 ◽  
Vol 17 (18) ◽  
pp. 6456
Author(s):  
Luiz Alberto Colnago ◽  
Iara Maria Trevisol ◽  
Daiane Voss Rech ◽  
Lucimara Aparecida Forato ◽  
Cirlei Igreja do Nascimento Mitre ◽  
...  
Keyword(s):  
Rna Virus ◽  
Low Cost ◽  
Virus Infectivity ◽  
Virucidal Activity ◽  
Lower Socioeconomic Status ◽  
Lower Socioeconomic ◽  
Dishwashing Detergent ◽  
Chicken Eggs ◽  
Embryonated Chicken ◽  
Virus Quantification

The COVID-19 infection, caused by SARS-CoV-2, is inequitably distributed and more lethal among populations with lower socioeconomic status. Direct contact with contaminated surfaces has been among the virus sources, as it remains infective up to days. Several disinfectants have been shown to inactivate SARS-CoV-2, but they rapidly evaporate, are flammable or toxic and may be scarce or inexistent for vulnerable populations. Therefore, we are proposing simple, easy to prepare, low-cost and efficient antiviral films, made with a widely available dishwashing detergent, which can be spread on hands and inanimate surfaces and is expected to maintain virucidal activity for longer periods than the current sanitizers. Avian coronavirus (ACoV) was used as model of the challenge to test the antivirus efficacy of the proposed films. Polystyrene petri dishes were covered with a thin layer of detergent formula. After drying, the films were exposed to different virus doses for 10 min and virus infectivity was determined using embryonated chicken eggs, and RNA virus quantification in allantoic fluids by RT-qPCR. The films inactivated the ACoV (ranging from 103.7 to 106.7 EID50), which is chemically and morphologically similar to SARS-CoV-2, and may constitute an excellent alternative to minimize the spread of COVID-19.

scholarly journals Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marloes Heijne ◽  
Martina Jelocnik ◽  
Alexander Umanets ◽  
Michael S. M. Brouwer ◽  
Annemieke Dinkla ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Opportunistic Pathogen ◽  
Chlamydia Psittaci ◽  
Phenotypic Analysis ◽  
Pathogenic Potential ◽  
The Family ◽  
Chicken Eggs ◽  
Sequence Types ◽  
Insight Into ◽  
Embryonated Chicken

AbstractChlamydia gallinacea is an obligate intracellular bacterium that has recently been added to the family of Chlamydiaceae. C. gallinacea is genetically diverse, widespread in poultry and a suspected cause of pneumonia in slaughterhouse workers. In poultry, C. gallinacea infections appear asymptomatic, but studies about the pathogenic potential are limited. In this study two novel sequence types of C. gallinacea were isolated from apparently healthy chickens. Both isolates (NL_G47 and NL_F725) were closely related to each other and have at least 99.5% DNA sequence identity to C. gallinacea Type strain 08-1274/3. To gain further insight into the pathogenic potential, infection experiments in embryonated chicken eggs and comparative genomics with Chlamydia psittaci were performed. C. psittaci is a ubiquitous zoonotic pathogen of birds and mammals, and infection in poultry can result in severe systemic illness. In experiments with embryonated chicken eggs, C. gallinacea induced mortality was observed, potentially strain dependent, but lower compared to C. psittaci induced mortality. Comparative analyses confirmed all currently available C. gallinacea genomes possess the hallmark genes coding for known and potential virulence factors as found in C. psittaci albeit to a reduced number of orthologues or paralogs. The presence of potential virulence factors and the observed mortality in embryonated eggs indicates C. gallinacea should rather be considered as an opportunistic pathogen than an innocuous commensal.

scholarly journals Simplified Method for Efficient Intravascular Inoculation of Chicken Embryos

1976 ◽  
Vol 4 (1) ◽  
pp. 104-105
Author(s):  
C. L. Kelling ◽  
I. A. Schipper
Keyword(s):  
Vascular Trauma ◽  
Chicken Embryos ◽  
Simplified Method ◽  
Chicken Eggs ◽  
Embryonated Chicken ◽  
Embryonic Death

The simple syringe-stabilizer unit described in this note provides a means for rapid intravascular inoculation of embryonated chicken eggs with minimal embryonic death from vascular trauma.

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