Protective Role of Peroxiredoxin I in Heat-Killed Staphylococcus Aureus-infected Mice

Staphylococcus aureus infection causes oxidative stress in neutrophils. The immune cells use reactive oxygen species (ROS) for carrying out their normal functions while an excess amount of ROS can attack cellular components that lead to cell damage. The present study was aimed to test the protective role of nanoconjugated vancomycin against vancomycin-sensitive Staphylococcus aureus (VSSA) and vancomycin-resistant Staphylococcus aureus (VRSA) infection induced oxidative stress in neutrophils. VSSA- and VRSA-infection were developed in Swiss mice by intraperitoneal injection of 5×106 CFU/mL bacterial solutions. Nanoconjugated vancomycin was treated to VSSA- and VRSA-infected mice at its effective dose for 10 days. Vancomycin was treated to VSSA and VRSA infected mice at similar dose, respectively, for 10 days. The result reveals that in vivo VSSA and VRSA infection significantly increases the level of lipid peroxidation, protein oxidation, oxidized glutathione level, and nitrite generation and decreases the level of reduced glutathione, antioxidant enzyme status, and glutathione-dependent enzymes as compared to control group; which were increased or decreased significantly near to normal in nanoconjugated vancomycin-treated group. These finding suggests the potential use and beneficial protective role of nanoconjugated vancomycin against VSSA and VRSA infection induced oxidative imbalance in neutrophils.

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Role of Neutrophil Leukocytes in Cutaneous Infection Caused by Staphylococcus aureus

Infection and Immunity ◽

10.1128/iai.68.11.6162-6167.2000 ◽

2000 ◽

Vol 68 (11) ◽

pp. 6162-6167 ◽

Cited By ~ 99

Author(s):

Lena Mölne ◽

Margareta Verdrengh ◽

Andrzej Tarkowski

Keyword(s):

Staphylococcus Aureus ◽

Monoclonal Antibody ◽

Great Majority ◽

Skin Lesions ◽

Protective Role ◽

Toxic Shock ◽

Cutaneous Infections ◽

High Prevalence ◽

Toxic Shock Syndrome Toxin

ABSTRACT Despite the high prevalence of cutaneous infections, little is known about the role of host immune responsiveness duringStaphylococcus aureus dermatitis. We have recently described a murine model of infectious dermatitis induced by superantigen-producing S. aureus. To assess the role of neutrophils in staphylococcal dermatitis, mice were given granulocyte-depleting monoclonal antibody prior to and on several occasions following intracutaneous inoculation with staphylococci. The granulocyte-depleted mice that had been intradermally inoculated withS. aureus developed crusted ulcerations which tended not to heal, whereas animals injected with control monoclonal antibody displayed only minor and transient skin lesions. The finding of severe ulcerations in neutropenic mice correlated with a significantly higher burden of bacteria in the blood and skin during the early phase of the infection. Importantly, while mice with an intact granulocyte population showed only limited skin infection, bacteremia occurred in the great majority of the neutrophil-depleted animals. As a consequence, the latter individuals exhibited significantly increased levels of the proinflammatory cytokine interleukin-6 and specific antibodies to staphylococcal cell wall components and toxic shock syndrome toxin-1 in the serum. Our data point to a crucial protective role of granulocytes in S. aureus dermatitis.

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Protective role of sialophorin (CD43)-expressing cells in experimental Staphylococcus aureus infection.

Infection and Immunity ◽

10.1128/iai.62.10.4637-4640.1994 ◽

1994 ◽

Vol 62 (10) ◽

pp. 4637-4640 ◽

Cited By ~ 7

Author(s):

T Bremell ◽

R Holmdahl ◽

A Tarkowski

Keyword(s):

Staphylococcus Aureus ◽

Protective Role ◽

Aureus Infection

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Protective role of NK1.1+ cells in experimental Staphylococcus aureus arthritis

Clinical & Experimental Immunology ◽

10.1046/j.1365-2249.1999.00922.x ◽

1999 ◽

Vol 117 (1) ◽

pp. 63-69 ◽

Cited By ~ 32

Author(s):

NILSSON ◽

BREMELL ◽

TARKOWSKI ◽

CARLSTEN

Keyword(s):

Staphylococcus Aureus ◽

Protective Role

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Protective role of IL-1β against post-arthroplasty Staphylococcus aureus infection

Journal of Orthopaedic Research® ◽

10.1002/jor.21414 ◽

2011 ◽

Vol 29 (10) ◽

pp. 1621-1626 ◽

Cited By ~ 39

Author(s):

Nicholas M. Bernthal ◽

Jonathan R. Pribaz ◽

Alexandra I. Stavrakis ◽

Fabrizio Billi ◽

John S. Cho ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Protective Role ◽

Aureus Infection

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Results of challenge of goats with Staphylococcus aureus into the teat of the udder

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15644 ◽

2018 ◽

Vol 67 (4) ◽

pp. 237

Author(s):

A. TZORA ◽

C. VOIDAROU ◽

A. KARAMOUTSIOS ◽

J. SKOUFOS

Keyword(s):

Staphylococcus Aureus ◽

Protective Role ◽

Aureus Strain ◽

Dairy Herds ◽

Cytological Examination ◽

Lactating Goats ◽

Before And After ◽

Group A ◽

Group B

Objective of the present study was to study the outcome of inoculation of Staphylococcus aureus into the teat duct of female goats, which simulates mammary natural infections. In total, 22 lactating goats were used in the study; 8 animals were challenged with a S. aureus strain at a depth of 2 mm into one teat duct (group A), 8 animals were challenged with the same strain at 6 mm into one teat duct (group B) and 6 animals were challenged directly into one gland cistern (group C). Challenge dose was always 1300 cfu. Animals were examined clinically before and after challenge; milk samples were collected for bacteriological and cytological examination, and milk yield measurements were also performed. Goats in group A or B developed a significantly milder response than animals in group C. It is concluded that the evidence indicates a protective role of the normal teat of the udder of goats and that the results also underline the significance of maintaining healthy teats for prevention of mastitis in dairy herds.

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