scholarly journals О молекулярной природе различий в реакции сенсорных нейронов и фибробластов на уабаин

2021 ◽  
Vol 91 (5) ◽  
pp. 882
Author(s):  
М.М. Халисов ◽  
В.А. Пеннияйнен ◽  
С.А. Подзорова ◽  
А.В. Анкудинов ◽  
К.И. Тимощук ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sensory Neurons ◽  
Intracellular Signaling ◽  
Mechanical Characteristics ◽  
Signaling Cascades ◽  
Force Microscopy ◽  
Atomic Force ◽  
Endogenous Ouabain ◽  
Membrane Rigidity ◽  
Pumping Function

Atomic force microscopy was used to study under physiologically adequate conditions the effect of ouabain on the mechanical characteristics of sensory neurons and fibroblasts of 10–12-day old chick embryos. Fibroblasts express only the α1-isoform of Na,K-ATPase, and sensory neurons the α1- and α3-isoforms. It was found that the action of ouabain at a concentration corresponding to the endogenous value leads to an increase in the membrane rigidity of sensory neurons, which is apparently due to the activation of the transducer function of Na,K-ATPase, rather than the pumping function. The endogenous concentration of ouabain did not change the mechanical characteristics of fibroblasts. The results obtained suggest that endogenous ouabain modulates the transducer function of the α3-isoform of Na,K-ATPase of the sensory neuron membrane. Thus, the method of atomic force microscopy allows a comparative study of intracellular signaling cascades in living cells.

scholarly journals Эффект длины зонда на кантилевере атомно-силового микроскопа в измерениях механических свойств нативных нейронов

2018 ◽  
Vol 44 (15) ◽  
pp. 38 ◽  
Author(s):  
А.В. Анкудинов ◽  
М.М. Халисов ◽  
В.А. Пеннияйнен ◽  
С.А. Подзорова ◽  
К.И. Тимощук ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sensory Neurons ◽  
Young Modulus ◽  
Beam Length ◽  
Qualitative Explanation ◽  
Force Microscopy ◽  
Physiological Conditions ◽  
Atomic Force

AbstractLiving sensory neurons were studied by atomic force microscopy in the PeakForce QNM mode under near-physiological conditions. The dependence of the measured apparent Young modulus of cells on the ratio of the probe height to the beam length on a used cantilever was revealed. A qualitative explanation based on the analysis of beam strains in two limit cases, in which the probe slides over a studied object and the probe sticks to the latter, was given to the obtained result. It was proposed to classify native cells by the character of their interaction with the probe (sliding or sticking).

Probing Mechanical Characteristics of Living Fibroblasts via Atomic Force Microscopy

2019 ◽  
Vol 45 (9) ◽  
pp. 947-950 ◽  
Author(s):  
K. I. Timoshchuk ◽  
M. M. Khalisov ◽  
V. A. Penniyaynen ◽  
B. V. Krylov ◽  
A. V. Ankudinov
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Characteristics ◽  
Force Microscopy ◽  
Atomic Force

Steel Pile-Up Characterization by Atomic Force Microscopy

2017 ◽  
Vol 891 ◽  
pp. 78-82 ◽  
Author(s):  
Peter Burik ◽  
Pavol Zubko ◽  
Ladislav Pešek ◽  
Lukáš Voleský
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Young’S Modulus ◽  
Mechanical Characteristics ◽  
The Real ◽  
Force Microscopy ◽  
Contact Depth ◽  
Steel Sheets ◽  
Atomic Force ◽  
Pile Up

The Oliver–Pharr method has extensively been adopted for measuring hardness and Young’s modulus by indentation techniques. However, the method assumes that the contact periphery sinks in, which limits the applicability to the materials pile-up [1]. In this work, we characterize the pile-up (shape and height) in steel sheets with different mechanical properties and propose an improved methodology to calculate the real mechanical characteristics of steel sheets with significant pile-up. Pile-up correction of mechanical characteristics is based on ratio of pile-up height and contact depth. Pile-up height was measured by atomic force microscopy (AFM).

Structural and Mechanical Characteristics of Exosomes from Osteosarcoma Cells Explored by 3D-Atomic Force Microscopy

Nanoscale ◽  
2021 ◽  
Author(s):  
Ayhan Yurtsever ◽  
Takeshi Yoshida ◽  
Arash Badami Behjat ◽  
Yoshinori Araki ◽  
Rikinari Hanayama ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Atomic Force Microscopy ◽  
Drug Delivery Systems ◽  
Mechanical Characteristics ◽  
Cell Communication ◽  
Delivery Systems ◽  
Osteosarcoma Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Potential Biomarkers

Exosomes have recently gained interest as mediators of cell-to-cell communication and as potential biomarkers for cancer and other diseases. They also have potential as nanocarriers for drug delivery systems. Therefore,...

Nanomechanics of Mice Sensory Neurons as Measured by Atomic Force Microscopy

2013 ◽  
Author(s):  
Marta Martin ◽  
Ouafa Benzina ◽  
Vivien Szabo ◽  
Thierry Cloitre ◽  
Frederic Scamps
Keyword(s):  
Atomic Force Microscopy ◽  
Sensory Neurons ◽  
Force Microscopy ◽  
Atomic Force

Pile-Up Correction of Mechanical Characteristics of Individual Phases in Various Steel by Depth Sensing Indentation

2015 ◽  
Vol 662 ◽  
pp. 7-10 ◽  
Author(s):  
Peter Burik ◽  
Ladislav Pešek ◽  
Lukáš Voleský
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Grain Boundaries ◽  
Mechanical Characteristics ◽  
Depth Sensing ◽  
The Real ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pile Up ◽  
Shape And Size

The Oliver–Pharr method has extensively been adopted for measuring hardness and elastic modulus by indentation techniques. However, the method assumes that the contact periphery sinks in, which limits the applicability to the materials pile-up [1]. This study proposes an improved methodology to calculate the real mechanical characteristics of individual phases in various steels with significant pile-up. Pile-up correction of mechanical characteristics is based on ratio of pile-up height and contact depth. Pile-up height was measured by atomic force microscopy (AFM). The effect of grain boundaries on the shape and size of the pile-up lobes was also discussed.

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