Effects of substrate stiffness on the mechanical characteristics of breast cells using atomic force microscopy

Atomic force microscopy was used to study under physiologically adequate conditions the effect of ouabain on the mechanical characteristics of sensory neurons and fibroblasts of 10–12-day old chick embryos. Fibroblasts express only the α1-isoform of Na,K-ATPase, and sensory neurons the α1- and α3-isoforms. It was found that the action of ouabain at a concentration corresponding to the endogenous value leads to an increase in the membrane rigidity of sensory neurons, which is apparently due to the activation of the transducer function of Na,K-ATPase, rather than the pumping function. The endogenous concentration of ouabain did not change the mechanical characteristics of fibroblasts. The results obtained suggest that endogenous ouabain modulates the transducer function of the α3-isoform of Na,K-ATPase of the sensory neuron membrane. Thus, the method of atomic force microscopy allows a comparative study of intracellular signaling cascades in living cells.

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Steel Pile-Up Characterization by Atomic Force Microscopy

Materials Science Forum ◽

10.4028/www.scientific.net/msf.891.78 ◽

2017 ◽

Vol 891 ◽

pp. 78-82 ◽

Cited By ~ 1

Author(s):

Peter Burik ◽

Pavol Zubko ◽

Ladislav Pešek ◽

Lukáš Voleský

Keyword(s):

Mechanical Properties ◽

Atomic Force Microscopy ◽

Young’S Modulus ◽

Mechanical Characteristics ◽

The Real ◽

Force Microscopy ◽

Contact Depth ◽

Steel Sheets ◽

Atomic Force ◽

Pile Up

The Oliver–Pharr method has extensively been adopted for measuring hardness and Young’s modulus by indentation techniques. However, the method assumes that the contact periphery sinks in, which limits the applicability to the materials pile-up [1]. In this work, we characterize the pile-up (shape and height) in steel sheets with different mechanical properties and propose an improved methodology to calculate the real mechanical characteristics of steel sheets with significant pile-up. Pile-up correction of mechanical characteristics is based on ratio of pile-up height and contact depth. Pile-up height was measured by atomic force microscopy (AFM).

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Nanomechanical sub-surface mapping of living biological cells by force microscopy

Nanoscale ◽

10.1039/c9nr03497h ◽

2019 ◽

Vol 11 (27) ◽

pp. 13089-13097 ◽

Cited By ~ 3

Author(s):

Lukas Stühn ◽

Anna Fritschen ◽

Joseph Choy ◽

Martin Dehnert ◽

Christian Dietz

Keyword(s):

Atomic Force Microscopy ◽

Biological Cells ◽

Surface Mapping ◽

Force Microscopy ◽

Nanomechanical Properties ◽

Atomic Force ◽

Breast Cells

Atomic force microscopy allows for the acquisition of depth-resolved nanomechanical properties deduced from maps of force–distance curves recorded on cancerous epithelial breast cells.

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Structural and Mechanical Characteristics of Exosomes from Osteosarcoma Cells Explored by 3D-Atomic Force Microscopy

Nanoscale ◽

10.1039/d0nr09178b ◽

2021 ◽

Author(s):

Ayhan Yurtsever ◽

Takeshi Yoshida ◽

Arash Badami Behjat ◽

Yoshinori Araki ◽

Rikinari Hanayama ◽

...

Keyword(s):

Drug Delivery ◽

Atomic Force Microscopy ◽

Drug Delivery Systems ◽

Mechanical Characteristics ◽

Cell Communication ◽

Delivery Systems ◽

Osteosarcoma Cells ◽

Force Microscopy ◽

Atomic Force ◽

Potential Biomarkers

Exosomes have recently gained interest as mediators of cell-to-cell communication and as potential biomarkers for cancer and other diseases. They also have potential as nanocarriers for drug delivery systems. Therefore,...

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Pile-Up Correction of Mechanical Characteristics of Individual Phases in Various Steel by Depth Sensing Indentation

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.662.7 ◽

2015 ◽

Vol 662 ◽

pp. 7-10 ◽

Cited By ~ 1

Author(s):

Peter Burik ◽

Ladislav Pešek ◽

Lukáš Voleský

Keyword(s):

Atomic Force Microscopy ◽

Elastic Modulus ◽

Grain Boundaries ◽

Mechanical Characteristics ◽

Depth Sensing ◽

The Real ◽

Force Microscopy ◽

Atomic Force ◽

Pile Up ◽

Shape And Size

The Oliver–Pharr method has extensively been adopted for measuring hardness and elastic modulus by indentation techniques. However, the method assumes that the contact periphery sinks in, which limits the applicability to the materials pile-up [1]. This study proposes an improved methodology to calculate the real mechanical characteristics of individual phases in various steels with significant pile-up. Pile-up correction of mechanical characteristics is based on ratio of pile-up height and contact depth. Pile-up height was measured by atomic force microscopy (AFM). The effect of grain boundaries on the shape and size of the pile-up lobes was also discussed.

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