scholarly journals Metal Biosorption Studies to Treat Combined Industrial Effluents Using P. chrysosporium

2006 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Sripathi Rao K ◽  
Ravindra P
Keyword(s):  
Large Scale ◽  
Specific Growth ◽  
Industrial Effluent ◽  
Fungal Growth ◽  
Industrial Effluents ◽  
White Rot ◽  
White Rot Fungus ◽  
Metal Biosorption ◽  
Optimum Ph ◽  
Pilot Tests

This work reports the treatability studies conducted on the combined industrial effluent sample by white rot fungus. The selected strain, Phanerochaete chrysosporium, a white rot fungus, was employed in reduction of toxic metals. The specific growth rate of the fungus was found to be in the range of 0.089-0.102 hr -1. Studies conducted on biosorption of metals showed that the dead fungal biomass was found to be more effective than living fungus. The optimum pH for the fungal growth was found to be at 4.5 but enhanced biosorption was at pH 6, especially for maximum reduction of hexavalent chromium to trivalent chromium. Laboratory-scale experiments for metal biosorption with this Basidiomycete showed encouraging results, which could be applied further to pilot tests and large-scale studies.

Biosorption of Combined Industrial Effluents using Phanerochaete Chrysosporium

2006 ◽  
Vol 4 (1) ◽  
Author(s):  
Ravindra Pogaku ◽  
Sripathi Kulkarni
Keyword(s):  
Growth Rate ◽  
Metal Ions ◽  
Phanerochaete Chrysosporium ◽  
Specific Growth ◽  
Industrial Effluent ◽  
Fungal Growth ◽  
Industrial Effluents ◽  
Maximum Reduction ◽  
Optimum Ph ◽  
Biosorption Equilibrium

The biosorption of eight different metals from aqueous solutions of combined industrial effluent by live or dead cells of Phanerochaete chrysosporium was investigated. Growth rate studies of P. chrysosporium were carried out in a selected medium as well as in the effluent. The specific growth rate of the fungus was in the range of 0.089–0.102 h–1 in the medium. The biosorption of metal ions increased as the initial concentration of metal ions increased in the medium. Biosorption equilibrium was established in about 1 h and the concentration of adsorbed metal ions did not change further with time. The equilibrium was well described by Langmuir, Freundlich and Reidlich – Peterson isotherms. The dead fungal biomass was more effective than living fungus for biosorption of metals. The optimum pH for the fungal growth was at 4.5 but enhanced Biosorption was at pH 6 for maximum reduction of hexavalent chromium to trivalent.

scholarly journals Pyrene and Chrysene Tolerance and Biodegradation Capability of Pleurotus Sajor-Caju

2018 ◽  
Vol 12 (1) ◽  
pp. 24-35 ◽  
Author(s):  
Giuliano Saiu ◽  
Stefania Tronci ◽  
Massimiliano Grosso ◽  
Enzo Cadoni ◽  
Nicoletta Curreli
Keyword(s):  
Liquid Medium ◽  
Copper Sulphate ◽  
Negative Impact ◽  
Fungal Growth ◽  
Tween 80 ◽  
White Rot ◽  
White Rot Fungus ◽  
Polycyclic Aromatic ◽  
Pleurotus Sajor Caju

Introduction: The present work focused on the biodegradation capability of a white-rot fungus, the Pleurotus sajor-caju, when exposed to polycyclic aromatic hydrocarbons. Methods: The research was carried out by using in vitro systems developed on Petri dishes, to evaluate the fungal tolerance to pyrene and chrysene, followed by experiments in liquid medium. The first experimental campaign was necessary to evaluate the conditions promoting fungal growth and tolerance (presence of surfactants, peptone, copper sulphate and lecithin) and it was designed and analysed using statistical techniques. Results: It was found that the fungal population growth is strongly inhibited by chrysene presence. On the other hand, pyrene had a mild negative impact on the mycelia growth, which seemed to be positively influenced by the presence of Tween 80 and copper sulphate. Starting from these results, the behaviour of Pleurotus sajor-caju in presence of pyrene was investigated in liquid medium. Results showed that the depletion of pyrene was evident during a period of 20 days, and removal efficiency was greater than 90%.

Evaluation of Eucalyptus grandis Hill ex Maiden biopulping with Ceriporiopsis subvermispora under non-aseptic conditions

Holzforschung ◽  
2008 ◽  
Vol 62 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Fernando Masarin ◽  
André Ferraz
Keyword(s):  
Large Scale ◽  
Corn Steep Liquor ◽  
Eucalyptus Grandis ◽  
Wood Chip ◽  
Wood Chips ◽  
White Rot ◽  
White Rot Fungus ◽  
Growth Promoters ◽  
Ceriporiopsis Subvermispora ◽  
Aseptic Conditions

Abstract In biopulping, efficient wood colonization by a selected white-rot fungus depends on previous wood chip decontamination to avoid the growth of primary molds. Although simple to perform in the laboratory, in large-scale biopulping trials, complete wood decontamination is difficult to achieve. Furthermore, the use of fungal growth promoters such as corn steep liquor enhances the risk of culture contamination. This paper evaluates the ability of the biopulping fungus Ceriporiopsis subvermispora to compete with indigenous fungi in cultures of fresh or poorly decontaminated Eucalyptus grandis wood chips. While cultures containing autoclaved wood chips were completely free of contaminants, primary molds grew rapidly when non-autoclaved wood chips were used, resulting in heavily contaminated cultures, regardless of the C. subvermispora inoculum/wood ratio evaluated (5, 50 and 3000 mg mycelium kg−1 wood). Studies on benomyl-amended medium suggested that the fungi involved competed by consumption of the easily available nutrient sources, with C. subvermispora less successful than the contaminant fungi. The use of acid-washed wood chips decreased the level of such contaminant fungi, but production of manganese peroxidase and xylanases was also decreased under these conditions. Nevertheless, chemithermomechanical pulping of acid-washed samples biotreated under non-aseptic conditions gave similar fibrillation improvements compared to samples subjected to the standard biodegradation process using autoclaved wood chips.

scholarly journals Decolorization of textile industry wastewater in solid state fermentation with Peach-Palm (Bactris gasipaes) residue

2018 ◽  
Vol 78 (4) ◽  
pp. 718-727 ◽  
Author(s):  
J. A. Chicatto ◽  
K. T. Rainert ◽  
M. J. Gonçalves ◽  
C. V. Helm ◽  
D. Altmajer-Vaz ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Industrial Effluent ◽  
Color Removal ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Bactris Gasipaes ◽  
Experimental Conditions ◽  
Peach Palm

Abstract In this work we have assessed the decolorization of textile effluents throughout their treatment in a solid-state fermentation (SSF) system. SSF assays were conducted with peach-palm (Bactris gasipaes) residue using the white rot fungus Ganoderma lucidum EF 31. The influence of the dye concentration and of the amounts of peach-palm residue and liquid phase on both the discoloration efficiency and enzyme production was studied. According to our results, independently of experimental conditions employed, laccase was the main ligninolytic enzyme produced by G. lucidum. The highest laccase activity was obtained at very low effluent concentrations, suggesting the existence of an inhibitory effect of higher concentrations on fungal metabolism. The highest percentage of color removal was reached when 10 grams of peach palm residue was moistened with 60 mL of the final effluent. In control tests carried out with the synthetic dye Remazol Brilliant Blue R (RBBR) decolorization efficiencies about 20% higher than that achieved with the industrial effluent were achieved. The adsorption of RBBR on peach-palm residue was also investigated. Equilibrium tests showed that the adsorption of this dye followed both Langmuir and Freundlich isotherms. Hence, our experimental results indicate that peach-palm residue is suitable substrate for both laccase production and color removal in industrial effluents.

Identification of iron-regulated cellular proteins, Fe3+-reducing and -chelating compounds, in the white-rot fungus Perenniporia medulla-panis

2007 ◽  
Vol 53 (12) ◽  
pp. 1323-1329 ◽  
Author(s):  
Valdeir Arantes ◽  
Adriane M.F. Milagres
Keyword(s):  
Cell Surface ◽  
Growth Phase ◽  
Iron Supplementation ◽  
Fungal Growth ◽  
White Rot ◽  
Cellular Proteins ◽  
White Rot Fungus ◽  
Initial Growth ◽  
Reducing Activity ◽  
Chelating Compounds

In this paper, we present the responses of the white-rot fungus Perenniporia medulla-panis to iron availability with regard to alterations in growth, expression of cellular proteins, Fe3+-reducing activity, and Fe3+chelators production. Iron supplementation stimulated fungal growth but did not result in a significant increase in biomass production. Catechol and hydroxamate derivatives were produced mainly under iron deficiency, and their productions were repressed under iron supplementation conditions. Perenniporia medulla-panis showed several cellular proteins in the range of 10–90 kDa. Some of them showed negative iron-regulation. Iron-supplemented medium also repressed both cell surface and extracellular Fe3+-reducing activities; however, the highest cell surface activity was detected at the initial growth phase, whereas extracellular activity increased throughout the incubation period. No significant production of chelators and extracellular Fe3+-reducing activity were observed within the initial growth phase, suggesting that the reduction of Fe3+to Fe2+is performed by ferrireductases.

Kinetic and thermodynamic studies for evaluation of adsorption capacity of fungal dead biomass for direct dye

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Asma Hassan ◽  
Haq Nawaz Bhatti ◽  
Munawar Iqbal ◽  
Arif Nazir
Keyword(s):  
Dye Degradation ◽  
Carbon Sources ◽  
Industrial Effluents ◽  
Nitrogen Sources ◽  
White Rot ◽  
White Rot Fungus ◽  
Dead Biomass ◽  
Degradation Of Dyes ◽  
Freundlich Adsorption Isotherm ◽  
Pseudo Second Order

AbstractThis study focuses on evaluation of degradation aptitude of white rot fungus (Coriolus versicolor) against Indosol Turquoise FBL dye. The outcome of numerous parameters including pH, temperature, carbon sources, nitrogen sources, C/N ratio and effect of dye concentration were studied. Maximum decolorization (99.896%) of Indosol Turquoise FBL was obtained by C. versicolor under optimized conditions. After three days, the maximum dye degradation (98%) was observed at pH 4 and 30 °C. Six carbon sources fructose, glucose, maltose, sucrose, rice bran and wheat bran were used and 96.66% degradation was observed by maltose at its optimum growth concentration (0.1 g/100 mL). Various nitrogen sources were employed for decolorization but ammonium nitrate decolorized dye up to 98.05%. The activity of three different enzymes laccase, Lignin peroxidase (LiP) and Manganese peroxidase (MnP) were calculated. The dead biomass of White rot fungus (WRF) was used for biosorption experiments. Maximum q (36 mg/g) was obtained at pH 2, at 30 °C using 0.05 g biosorbent. An increase in the q value was observed with increase in dye concentration. Freundlich adsorption isotherm and pseudo second order kinetics were followed by the data. It can be concluded that C. versicolor could be an efficient source for degradation of dyes from industrial effluents.

scholarly journals The use of Phanerochaete chrysosporium for modification of bentonite for preconcentration and determination of heavy metals

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sevgi Kocaoba ◽  
Mehtap Duman Parlak ◽  
Munevver Arisoy
Keyword(s):  
Metal Ions ◽  
Phanerochaete Chrysosporium ◽  
Solid Phase ◽  
Atomic Absorption Spectrophotometry ◽  
Sample Volume ◽  
White Rot ◽  
White Rot Fungus ◽  
Sorption Data ◽  
Metal Biosorption

AbstractIn this study, a solid phase extraction method was successfully applied in the preconcentration and determination of trace levels of Cu(II) and Cd(II) ions and Phanerochaete chrysosporium (white rot fungus) modified bentonite was used as adsorbent. After the biosorption of Cu(II) and Cd(II) ions, metal concentrations in the samples were determined by atomic absorption spectrophotometry. pH, adsorbent amount, eluent type, sample volume, and flow rate, which are effective in the adsorption of metal ions, have been studied. 1 M HCl was used for desorption of these metal ions retained (recovery 95–100%). In addition, the effect of interfered ions has also been investigated. Sorption data were examined according to Langmuir and Freundlich adsorption equations. The results obtained show that the applied method has a high metal biosorption capacity, and Cu(II) and Cd(II) ions are successfully recovered. It was also successful in applying the proposed enrichment method to real water samples.Recovery values between 92.3% and 97.3% were obtained for the studied metal ions. According to the results, the proposed method can be successfully applied to water analysis at 95% confidence interval.

Characterization of a Laccase from White Rot Fungus Cerrena unicolor

2013 ◽  
Vol 442 ◽  
pp. 120-124
Author(s):  
Bei Bei Zhang ◽  
Yong Jian Shen ◽  
Chang Xu ◽  
Ying Li ◽  
Ming Hu Jiang
Keyword(s):  
Dye Decolorization ◽  
White Rot ◽  
White Rot Fungus ◽  
Optimum Temperature ◽  
Complex Chemical ◽  
Chemical Structures ◽  
Cerrena Unicolor ◽  
Sds Page ◽  
Optimum Ph

Dyes are usually difficult to be decolorized due to their complex chemical structures. In this work, laccase was purified from the white rot fungus Cerrena unicolor to evaluate its application in dye decolorization. SDS-PAGE analysis showed the purified laccase to be a monomeric protein of 63.7 kDa. The optimum pH for the oxidation of 2,2-azinobis-(3-ethylbenzthiaoline-6-sufonic acid) (ABTS) was 2.2 and the optimum temperature was 50°C. The activity of the purified enzyme was strongly inhibited by sodium azide and partially inhibited by cysteine, dithiothreitol. The Km values of the purified laccase for the substrate ABTS, syringaldazine and 2,6-dimethoxyphenol were 0.217, 0.306 and 0.199 mmol/L.

scholarly journals Diverse application and future prospects for commercial cultivation of microalgae species: A review

2019 ◽  
Vol 6 (4) ◽  
pp. 427-432
Author(s):  
Nishesh Sharma ◽  
Ajay Singh ◽  
Felicia Lalremruati ◽  
_ Vanlalmalsawmi ◽  
Rohit Sharma
Keyword(s):  
Natural Resources ◽  
Industrial Development ◽  
Large Scale ◽  
Algal Species ◽  
Industrial Effluent ◽  
Industrial Effluents ◽  
Polluted Water ◽  
Widespread Application ◽  
Commercial Cultivation ◽  
Wide Range

Industrial revolutions, advancements in health care, pharmaceuticals, transportation can be attributed to advancements made in the field of science and technology. Environment and natural resources has paid a heavy cost for most of industrial development. Rapid depletion of non-renewable sources of energy eventually leading towards the energy crisis, direct or indirect release of industrial effluents into soil and natural water bodies, global warming are among major consequences of industrialization. Ever since these environmental concerns have been recognized substantial studies have been conducted to minimize, control pollution and restore environment and natural resources. Among several measures cultivation of algae on large scale stands out to be a multipurpose solution. Inherent potential of microalgae species to accumulate lipids makes algae an efficient source of biofuel. Beside this ability of algae to detoxify polluted water and industrial effluent support utilization of algae for environment management and restoration. Efficient CO2 fixation, ability to tolerate wide range of environmental conditions, minimal nutritional requirements further support commercial cultivation of algal species to achieve their widespread application. However, efforts are required to develop large scale cultivation protocols (beyond the range of photobioreactors) so as to achieve practical applicability of algae and their products. Alongwith, cultivation protocols there is simultaneous need of either selection of naturally occurring high yielding strains / species or genetic improvement. Standardization of optimum cultivation conditions along with harvesting procedure is equally important.

scholarly journals PURIFICATION AND CHARACTERIZATION OF LACCASE INVOLVED IN THE DECOLOURIZATION OF SYNTHETIC DYES AND 2,3,7,8-TCDD CONGENER DEGRADATION BY THE WHITE ROT FUNGUS ISOLATED FROM BAVI FOREST OF VIETNAM

2018 ◽  
Vol 55 (4C) ◽  
pp. 180 ◽  
Author(s):  
Phung Khac Huy Chu
Keyword(s):  
Enzymatic Activity ◽  
Metal Ions ◽  
Textile Industry ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Sds Page ◽  
Extracellular Laccase ◽  
Optimum Ph

The fungal strain FBV40 was isolated from soil containing decayed wood in Ba Vi National Forest and capable of producing an extracellular laccase in the TSH1 medium. Two isozyme such as Lac1 and Lac2 were purified were estimated to be 55 and 60 kDa by SDS-PAGE. The optimum pH and temperature for the enzymatic activity of Lac1 were 3.0 and 60°C with ABTS using as the substrate. Kinetic constants Km and Vmax of Lac 1 were 0.3 µM and 200,000 µM/mins with ABTS as substrate. Cl-, SDS, and EDTA at any concentration (2 mM; 5 mM and 10 mM) strongly inhibited the activity of laccase. The enzyme was stable in the presence of several metal ions including Ni2+ (1 mM), Cu2+ (1 mM and 3 mM), Ca2+ (3 mM and 4 mM); in the presence of Cu2+ (2 mM) and Ca2+ (0.5 mM, 1.0 mM and 2.0 mM), laccase even showed the increase in the activity. The presence of metal ions Mn2+, Mg2+, Fe2+ completely inhibited the enzymatic activity at any examined concentration. The crude enzyme, as well as Lac 1, was able to decolourization MN.FBN dye from the textile industry from Ministry of Defence. This strain was able to degrade 2,3,7,8-TCDD isotop with initial concentration 2,000 ng-TEQ/L at rates over 46.8 % after ten days cultivation in the TSH1 medium. In the presence of three strains FBV40, FBVLa1 and FBD154 with the ratio 1:1:1, the degradation of this congener was achieved more than 95 % at the same time cultivation.

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