scholarly journals Rotating Biological Contactor for Biological Treatment of Poultry Processing Plant Wastewater Using Saccharomyces Cerevisiae

2008 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Dr. Gashem Najafpour ◽  
Punita Nook Naidu ◽  
Azlina Harun Kamaruddin

Biological treatment using attached growth on a rotating biological contactor(RBC) was implemented for wastewater from poultry industries, which containsa high level of organic compounds due to the slaughtering, rendering of bonesand fats, and plucking processes. The wastewater mostly consists of proteins,blood, fat and feathers. Nutrients available in the wastewater may enhance thegrowth of microorganisms, thus, it allows biological treatment to be usedeffectively. On the other hand, there are problems associated with biologicaltreatment processes such as nutrients promoting algae growth. The controlvariables for the attached growth in RBC were wastewater detention time, effectivesurface for attached growth, disc submerged level in the wastewater basin, shaftrotation for aeration, pH control and supplementary nutrients. The attachedgrowth resulted to high COD refPoval. The best treatment was obtained after 24hours with 29 % disc submergence level. The effective surface area for cellgrowth was 10.7 m2 using 60 discs mounted on a shaft. The microorganismused for the attached microbial growth was Saccharomyces cerevisiae. The shaftwas rotating at 11 rpm. The treatment was improved with the addition of 1 v/vof 0.1 molar sodium hydroxide solution to the wastewater for controlling pH. A91 % COD removal was obtained with RBC operating at optimum conditions,with a DO concentration of 3.98 mg/l.Key words: Rotating biological contactors, Aerobic treatment, Poultry processing wastewater,Saccharomyces cereuisiae,Attached growth.

2014 ◽  
Vol 10 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Heather M. Nelson ◽  
Rakesh K. Singh ◽  
Ramesh Y. Avula ◽  
Romeo T. Toledo

Abstract Membrane bioreactor (MBR) provided with spiral wound modules of polyacrylonitrile ultrafiltration membranes was used to treat the wastewater obtained from primary and secondary processing operations of a poultry plant. The membrane bioreactor consisted of 3 tanks; an aerobic bioreactor, anoxic settling tank, and a third tank from which a permeate was drawn across ultrafiltration membranes for final discharge to a municipal sewer or for reuse in the processing of raw product. The Cleaning and backflush schedules were conducted to determine the best regimen for maximum permeate flux and for their effect on retention times in each of the biological treatment tanks. Continuous operation of MBR for 24 h period with no backflushing resulted in flux decay that led to a substantial change in retention times. The best operating cycle was found to be 1 h filtration followed by 120 s backflush or 20 min filtration followed by 20 s backflush. Significant reduction in particle size, COD and BOD (>90%) and reduction of microbial load by 4 – log levels in MBR effluent made it fit for reuse.


1999 ◽  
Vol 65 (9) ◽  
pp. 3828-3833 ◽  
Author(s):  
Ifigenia Geornaras ◽  
Nokuthula F. Kunene ◽  
Alexander von Holy ◽  
John W. Hastings

ABSTRACT Molecular typing has been used previously to identify and trace dissemination of pathogenic and spoilage bacteria associated with food processing. Amplified fragment length polymorphism (AFLP) is a novel DNA fingerprinting technique which is considered highly reproducible and has high discriminatory power. This technique was used to fingerprint 88 Pseudomonas fluorescens andPseudomonas putida strains that were previously isolated from plate counts of carcasses at six processing stages and various equipment surfaces and environmental sources of a poultry abattoir. Clustering of the AFLP patterns revealed a high level of diversity among the strains. Six clusters (clusters I through VI) were delineated at an arbitrary Dice coefficient level of 0.65; clusters III (31 strains) and IV (28 strains) were the largest clusters. More than one-half (52.3%) of the strains obtained from carcass samples, which may have represented the resident carcass population, grouped together in cluster III. By contrast, 43.2% of the strains from most of the equipment surfaces and environmental sources grouped together in cluster IV. In most cases, the clusters in which carcass strains from processing stages grouped corresponded to the clusters in which strains from the associated equipment surfaces and/or environmental sources were found. This provided evidence that there was cross-contamination between carcasses and the abattoir environment at the DNA level. The AFLP data also showed that strains were being disseminated from the beginning to the end of the poultry processing operation, since many strains associated with carcasses at the packaging stage were members of the same clusters as strains obtained from carcasses after the defeathering stage.


1986 ◽  
Vol 18 (7-8) ◽  
pp. 289-296
Author(s):  
C. F. Ouyang ◽  
T. J. Wan

This study investigated and compared the treatment characteristics of three different kinds of biological wastewater treatment plants (including rotating biological contactor, trickling filter and oxidation ditch) which are currently operated in Taiwan. The field investigation of this study concentrated on the following items: the performance of biological oxygen demand (BOD) and suspended solids (SS) removal; the sludge yield rate of BOD removal; the settleability of sludge solids; the properties of sludge thickening; the power consumption and land area requirement per unit volume of wastewater. Finally, based on the results of the field investigation, a comparison of the treatment characteristics of the three different biological treatment processes was evaluated.


Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1341-1351 ◽  
Author(s):  
I King Jordan ◽  
John F McDonald

Abstract The Saccharomyces cerevisiae genome contains five families of long terminal repeat (LTR) retrotransposons, Ty1–Ty5. The sequencing of the S. cerevisiae genome provides an unprecedented opportunity to examine the patterns of molecular variation existing among the entire genomic complement of Ty retrotransposons. We report the results of an analysis of the nucleotide and amino acid sequence variation within and between the five Ty element families of the S. cerevisiae genome. Our results indicate that individual Ty element families tend to be highly homogenous in both sequence and size variation. Comparisons of within-element 5′ and 3′ LTR sequences indicate that the vast majority of Ty elements have recently transposed. Furthermore, intrafamily Ty sequence comparisons reveal the action of negative selection on Ty element coding sequences. These results taken together suggest that there is a high level of genomic turnover of S. cerevisiae Ty elements, which is presumably in response to selective pressure to escape host-mediated repression and elimination mechanisms.


1995 ◽  
Vol 35 (2) ◽  
pp. 73-82 ◽  
Author(s):  
Ifigenia Geornaras ◽  
Amelia De Jesus ◽  
Elsabé Van Zyl ◽  
Alexander Von Holy

1987 ◽  
Vol 7 (12) ◽  
pp. 4441-4452
Author(s):  
M Marshall ◽  
D Mahoney ◽  
A Rose ◽  
J B Hicks ◽  
J R Broach

The product of the Saccharomyces cerevisiae SIR4 gene, in conjunction with at least three other gene products, prevents expression of mating-type genes resident at loci at either end of chromosome III, but not of the same genes resident at the MAT locus in the middle of the chromosome. To address the mechanism of this novel position effect regulation, we have conducted a structural and genetic analysis of the SIR4 gene. We have determined the nucleotide sequence of the gene and found that it encodes a lysine-rich, serine-rich protein of 152 kilodaltons. Expression of the carboxy half of the protein complements a chromosomal nonsense mutation of sir4 but not a complete deletion of the gene. These results suggest that SIR4 protein activity resides in two portions of the molecule, but that these domains need not be covalently linked to execute their biological function. We also found that high-level expression of the carboxy domain of the protein yields dominant derepression of the silent loci. This anti-Sir activity can be reversed by increased expression of the SIR3 gene, whose product is normally also required for maintaining repression of the silent loci. These results are consistent with the hypothesis that SIR3 and SIR4 proteins physically associate to form a multicomponent complex required for repression of the silent mating-type loci.


2015 ◽  
Vol 73 (5) ◽  
pp. 1202-1210 ◽  
Author(s):  
G. Cema ◽  
S. Żabczyński ◽  
A. Ziembińska-Buczyńska

Coke wastewater is known to be relatively difficult for biological treatment. Nonetheless, biofilm-based systems seem to be promising tool for such treatment. That is why a rotating biological contactor (RBC) system focused on the Anammox process was used in this study. The experiment was divided into two parts with synthetic and then real wastewater. It was proven that it is possible to treat coke wastewater with RBC but such a procedure requires a very long start-up period for the nitritation (190 days), as well as for the Anammox process, where stable nitrogen removal over 70% was achieved after 400 days of experiment. Interestingly, it was possible at a relatively low (20.2 ± 2.2 °C) temperature. The polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) based monitoring of the bacterial community showed that its biodiversity decreased when the real wastewater was treated and it was composed mainly of GC-rich genotypes, probably because of the modeling influence of this wastewater and the genotypes specialization.


Author(s):  
Ummu Afiqah Abdul-Rahiman ◽  
Noordiana Nordin ◽  
Noor Azira Abdul-Mutalib ◽  
Maimunah Sanny

Salmonella are widely found in the poultry industry, which subsequently may pose a risk to animal and human health. The aim of this review is to highlight strategies for the prevention and control of Salmonella at each stage in the poultry production chain by monitoring risks from the farm to the retailer. Among the primary approaches for control of Salmonella at the farm level includes the administration of synthetic and natural compounds to live chickens (vaccination and antibiotic), litter management as well as fortification of feed and acidification of drinking water. In the poultry processing plant, multiple hurdle technology and different chilling conditions to reduce Salmonella were discussed. In the retail level, an effective monitoring program to control Salmonella contamination by good manufacturing practices and hazard analysis and critical control points has been reviewed. Overall, we conclude that these approaches play a role in reducing the dissemination of Salmonella in the poultry industry. However, there is no published data related to logistic scheduling of poultry processing.


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