Optimization of Anthocyanin Extraction from Cockspur Coral (Erythrina Crista-Galli L.) Petals with Microwave-Assisted Extraction (MAE) using Response Surface Methodology

Cockspur coral (Erythrina crista-galli L) petals are flowers that contain anthocyanins and active compounds of flavonoids and tannins. This study aims to determine the optimum conditions for the anthocyanin extraction process of cockspur coral petals using Microwave-Assisted Extraction (MAE), analyzed through the Response Surface Method (RSM). This process uses ethanol-hydrochloric acid solvents and a Box-Behnken experimental design involving three factors, namely the solvent ratios (w/v) (1:5, 1:15, and 1:25), microwave powers (300, 450, and 600 watts), and extraction times (3, 9, and 15 minutes). As a result, the second-order polynomial model was enhanced and sufficient to explain the variation of the data that denoted the significant correlation with the independent variables and the response. Derringer's desired function methodology was used for optimizing studies and generated ideal conditions for each or combined an independent variable. The optimum anthocyanin extract of 5.82 mg/L was obtained at a power condition of 325,5 Watts, an extraction time of 3.05 minutes, and a solvent ratio of 20.5. Meanwhile, the operating conditions at a power of 310.8 Watts, a time of 14.94 minutes, and a solvent ratio of 24.96 resulted in the optimum color intensity (IC) of 1040.26. In the meantime, the optimum antioxidant activity was obtained at a power of 585.97 Watts, a time of 4.93 minutes, and a solvent ratio of 5.43 with IC50 of 0.115.

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Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery (Apium graveolens L.) leaves by response surface methodology

Czech Journal of Food Sciences ◽

10.17221/266/2015-cjfs ◽

2016 ◽

Vol 34 (No. 4) ◽

pp. 341-349 ◽

Cited By ~ 5

Author(s):

Q. He ◽

Y. Li ◽

P. Zhang ◽

A. Zhang ◽

H. Wu

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Ethanol Concentration ◽

Apium Graveolens ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Inhibition Rate ◽

Microwave Assisted ◽

Assisted Extraction

The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.

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Optimisasi Metode Microwave-Assisted Extraction Terhadap Kadar Kuersetin dari Limbah Kulit Bawang Merah (Allium cepa L.)

FITOFARMAKA: Jurnal Ilmiah Farmasi ◽

10.33751/jf.v10i2.2154 ◽

2020 ◽

Vol 10 (2) ◽

pp. 122-131

Author(s):

Zaldy Rusli ◽

Nina Herlina ◽

Bina Lohita Sari ◽

Salma Hanunah Ulfa

Keyword(s):

Response Surface ◽

Allium Cepa ◽

Response Surface Method ◽

Microwave Assisted Extraction ◽

Design Expert ◽

Microwave Assisted ◽

Surface Method ◽

Assisted Extraction ◽

Allium Cepa L

Peningkatan produksi dan penggunaan bawang merah dimasyarakat tentunya menyebabkan peningkatan jumlah limbah dari kulit bawang merah yang jarang dimanfaatkan masyarakat. Kulit bawang merah memiliki banyak kandungan senyawa aktif yang bermanfaat bagi kesehatan, salah satunya adalah kuersetin yang memiliki manfaat mengatasi hiperglikemia, anti-inflamasi, antioksidan bahkan sebagai antikanker. Penelitian ini bertujuan untuk melakukan optimisasi proses ekstraksi kuersetin yang terkandung dalam kulit bawang merah. Proses ekstraksi dilakukan menggunakan Microwave-Assisted Extraction (MAE) dengan parameter daya microwave, waktu ekstraksi dan konsentrasi etanol. Analisis kadar kuersetin dilakukan menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). Analisis data dilakukan menggunakan Response Surface Method (RSM) dengan menggunakan perangkat lunak Design Expert 12. Kondisi optimum metode MAE untuk mengekstraksi kuersetin dari kulit bawang merah adalah dengan menggunakan microwave dengan daya 450 watt selama 110,57 detik dan dengan menggunakan etanol 89,078%. Pada kondisi tersebut, diperoleh kuersetin dengan kadar 7,3933 mg/g serbuk simplisia kulit bawang merah. Dari data ini, dapat disimpulkan bahwa kulit bawang merah mengandung kuersetin yang dapat dimanfaatkan sebagai bahan obat.

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Optimization of microwave-assisted extraction process for blue honeysuckle (Lonicera coerulea L.) using response surface methodology

Korean Journal of Food Preservation ◽

10.11002/kjfp.2017.24.5.623 ◽

2017 ◽

Vol 24 (5) ◽

pp. 623-630 ◽

Cited By ~ 1

Author(s):

Daehee Park ◽

Jae-Jun Lee ◽

Jongjin Park ◽

Sanghwan Park ◽

Wonyoung Lee

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Extraction Process ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Blue Honeysuckle

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Optimasi Proses Ekstraksi Daun Sirsak (Annona muricata L) Metode MAE (Microwave Assisted Extraction) Dengan Respon Aktivitas Antioksidan Dan Total Fenol

JURNAL AGROINDUSTRI HALAL ◽

10.30997/jah.v4i1.1142 ◽

2018 ◽

Vol 4 (1) ◽

Author(s):

Latifa Putri Aulia

Keyword(s):

Optimum Condition ◽

Extraction Process ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Optimum Point ◽

A Value ◽

Assisted Extraction ◽

Anti Cancer ◽

Anti Oxidant

Soursop leaf (Annona muriciata L) contain active ingredients that is annonain, saponin, flavonoid, and tannin. Some research even found that in soursop leaf contain bio active substance called acetogenin that act as anti cancer. Commonly extraction process used to extracting substance in the leaf is known as conventional extracting process which has flaws. Hence its need further research in term of extracting in which more optimal that is the usage of microwave (Microwave Assisted Extraction / MAE) MAE extraction is extracting process that using microwave radiation to heat the solvent quick and efficient so the extraction can be done in time to extract selectively from various raw ingredients.Response Surface Methodolgy (RSM) is a an appropriate method to analyze the effects of a single variable and for seeking the optimum condition for multivariable systems efficiently. The purpose of this research is to determine the optimal condition from soursop leaf extraction using MAE extraction with 2 variables that is extraction time and solvent ratio so can be concluded that rough soursop leaf extract with the optimal phenol and characteristics anti oxidant activities. This research using CCD method from RSM (Response Surface Methodology) with 2 unbound variables that time extraction (X1) and solvent ratio (X2). Earned result from this research is quadratic with the equation is a value for antioxidant activity Y = -2,24087 + 2,17790X1 + 5,25566X2 + 0,12000X1X2 - 0,25875X12 – 0,12373X22. While the total value for phenol is Y = -175,82270 – 8,07421X1 + 42,0810X2 – 2,62317X1X2 – 3,00785X12 -1,39204 X22. The optimum point from each variable is the extracting time 9' and 84" with solvent ratio of 25,19 of ingredient that is simplicia powder of soursop leaf approximately 25gr. From the optimum point we can drawn that optimum condition of anti oxidant activity respond is as much as 75,75% and phenol total value of 276,9 ppm. From the analysis result we can conclude that extracting time and solvent ratio can signnificanly effect the result we get.

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Microwave-assisted extraction of phenolic compounds from coffee (Coffea robusta L. Linden) bee pollen

Herba Polonica ◽

10.2478/hepo-2021-0015 ◽

2021 ◽

Vol 67 (3) ◽

pp. 37-44

Author(s):

Le Pham Tan Quoc

Keyword(s):

Phenolic Compounds ◽

Extraction Process ◽

Solvent Ratio ◽

Scanning Electron Micrographs ◽

Microwave Assisted Extraction ◽

Bee Pollen ◽

Microwave Assisted ◽

Assisted Extraction ◽

Optimal Extraction Condition ◽

Coffea Robusta

Summary Introduction: Coffee bee pollen contains some precious bioactive compounds, especially phenolic compounds. This material is easily found in many regions in Vietnam and is quite useful for human health. Objective: The main aim of this study was to determine the best extraction conditions for the total polyphenol content (TPC) and antioxidant capacity (AC) of coffee (Coffea robusta L. Linden) bee pollen with microwave-assisted extraction (MAE). Methods: TPC and AC of extract of coffee (C. robusta) bee pollen were determined using Folin-Ciocalteu (FC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. In addition, micromorphology of sample was observed using scanning electron micrographs (SEM). Results: The findings showed that all factors strongly affected the efficiency of the extraction process. The best extraction conditions obtained were ethanol concentration of 60% (v/v), solid to solvent ratio of 1/30 (w/v), extraction time of 7 min, and a microwave power of 314 W. Conclusion: The highest TPC and AC obtained were 13.73 mg GAE/g DW and 10.08 µmol TE/g DW with aqueous ethanol as the solvent at the optimal extraction condition. In addition, the microstructures of the material changed insignificantly under microwave irradiation.

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