Optimasi Proses Ekstraksi Daun Sirsak (Annona muricata L) Metode MAE (Microwave Assisted Extraction) Dengan Respon Aktivitas Antioksidan Dan Total Fenol

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Latifa Putri Aulia
Keyword(s):  
Optimum Condition ◽  
Extraction Process ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Optimum Point ◽  
A Value ◽  
Assisted Extraction ◽  
Anti Cancer ◽  
Anti Oxidant

Soursop leaf (Annona muriciata L) contain active ingredients that is annonain, saponin, flavonoid, and tannin. Some research even found that in soursop leaf contain bio active substance called acetogenin that act as anti cancer. Commonly extraction process used to extracting substance in the leaf is known as conventional extracting process which has flaws. Hence its need further research in term of extracting in which more optimal that is the usage of microwave (Microwave Assisted Extraction / MAE) MAE extraction is extracting process that using microwave radiation to heat the solvent quick and efficient so the extraction can be done in time to extract selectively from various raw ingredients.Response Surface Methodolgy (RSM) is a an appropriate method to analyze the effects of a single variable and for seeking the optimum condition for multivariable systems efficiently. The purpose of this research is to determine the optimal condition from soursop leaf extraction using MAE extraction with 2 variables that is extraction time and solvent ratio so can be concluded that rough soursop leaf extract with the optimal phenol and characteristics anti oxidant activities. This research using CCD method from RSM (Response Surface Methodology) with 2 unbound variables that time extraction (X1) and solvent ratio (X2). Earned result from this research is quadratic with the equation is a value for antioxidant activity  Y = -2,24087 + 2,17790X1 + 5,25566X2 + 0,12000X1X2 - 0,25875X12 – 0,12373X22. While the total value for phenol is Y = -175,82270 – 8,07421X1 + 42,0810X2 – 2,62317X1X2 – 3,00785X12 -1,39204 X22. The optimum point from each variable is the extracting time 9' and 84" with solvent ratio of 25,19 of ingredient that is simplicia powder of soursop leaf approximately 25gr. From the optimum point we can drawn that optimum condition of anti oxidant activity respond is as much as 75,75% and phenol total value of 276,9 ppm. From the analysis result we can conclude that extracting time and solvent ratio can signnificanly effect the result we get.

scholarly journals Optimization of Anthocyanin Extraction from Cockspur Coral (Erythrina Crista-Galli L.) Petals with Microwave-Assisted Extraction (MAE) using Response Surface Methodology

2021 ◽  
Vol 21 (2) ◽  
pp. 143
Author(s):  
Astrilia Damayanti ◽  
Bayu Triwibowo ◽  
Megawati Megawati ◽  
Miftahuddin Azhari ◽  
Sandra Anggita Fadriana
Keyword(s):  
Response Surface ◽  
Extraction Process ◽  
Operating Conditions ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Surface Method ◽  
Assisted Extraction ◽  
Order Polynomial ◽  
Independent Variable

Cockspur coral (Erythrina crista-galli L) petals are flowers that contain anthocyanins and active compounds of flavonoids and tannins. This study aims to determine the optimum conditions for the anthocyanin extraction process of cockspur coral petals using Microwave-Assisted Extraction (MAE), analyzed through the Response Surface Method (RSM). This process uses ethanol-hydrochloric acid solvents and a Box-Behnken experimental design involving three factors, namely the solvent ratios (w/v) (1:5, 1:15, and 1:25), microwave powers (300, 450, and 600 watts), and extraction times (3, 9, and 15 minutes). As a result, the second-order polynomial model was enhanced and sufficient to explain the variation of the data that denoted the significant correlation with the independent variables and the response. Derringer's desired function methodology was used for optimizing studies and generated ideal conditions for each or combined an independent variable.  The optimum anthocyanin extract of 5.82 mg/L was obtained at a power condition of 325,5 Watts, an extraction time of 3.05 minutes, and a solvent ratio of 20.5. Meanwhile, the operating conditions at a power of 310.8 Watts, a time of 14.94 minutes, and a solvent ratio of 24.96 resulted in the optimum color intensity (IC) of 1040.26. In the meantime, the optimum antioxidant activity was obtained at a power of 585.97 Watts, a time of 4.93 minutes, and a solvent ratio of 5.43 with IC50 of 0.115.

scholarly journals Microwave-assisted extraction of phenolic compounds from coffee (Coffea robusta L. Linden) bee pollen

2021 ◽  
Vol 67 (3) ◽  
pp. 37-44
Author(s):  
Le Pham Tan Quoc
Keyword(s):  
Phenolic Compounds ◽  
Extraction Process ◽  
Solvent Ratio ◽  
Scanning Electron Micrographs ◽  
Microwave Assisted Extraction ◽  
Bee Pollen ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Optimal Extraction Condition ◽  
Coffea Robusta

Summary Introduction: Coffee bee pollen contains some precious bioactive compounds, especially phenolic compounds. This material is easily found in many regions in Vietnam and is quite useful for human health. Objective: The main aim of this study was to determine the best extraction conditions for the total polyphenol content (TPC) and antioxidant capacity (AC) of coffee (Coffea robusta L. Linden) bee pollen with microwave-assisted extraction (MAE). Methods: TPC and AC of extract of coffee (C. robusta) bee pollen were determined using Folin-Ciocalteu (FC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. In addition, micromorphology of sample was observed using scanning electron micrographs (SEM). Results: The findings showed that all factors strongly affected the efficiency of the extraction process. The best extraction conditions obtained were ethanol concentration of 60% (v/v), solid to solvent ratio of 1/30 (w/v), extraction time of 7 min, and a microwave power of 314 W. Conclusion: The highest TPC and AC obtained were 13.73 mg GAE/g DW and 10.08 µmol TE/g DW with aqueous ethanol as the solvent at the optimal extraction condition. In addition, the microstructures of the material changed insignificantly under microwave irradiation.

Orthogonal test in optimizing microwave-assisted extraction process of magnolin from Magnoliae Flos

2010 ◽  
Vol 30 (5) ◽  
pp. 567-568
Author(s):  
Xiao-li LI ◽  
Ming-yuan ZHANG ◽  
Wei-quan ZHAO ◽  
Man Li ◽  
Hai-ying TENG ◽  
...  
Keyword(s):  
Extraction Process ◽  
Orthogonal Test ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

scholarly journals Ekstraksi Polifenol Total dari Daun Kadamba (Mitragyna speciosa Korth) Menggunakan Metode Choline Chloride-Sorbitol based Microwave Assisted Extraction

2019 ◽  
Vol 10 ◽  
pp. 131-134
Author(s):  
Ramila Hidayati ◽  
Dewi Rahmawati ◽  
Islamudin Ahmad
Keyword(s):  
Choline Chloride ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Green Solvent ◽  
Microwave Assisted ◽  
Extract Solution ◽  
Mitragyna Speciosa ◽  
Assisted Extraction ◽  
Total Extraction

This research was conducted to determine the influence of the use of the choline chloride-sorbitol based microwave assisted extraction (MAE) method against the total extraction of polyphenols from the leaf Kadamba (Mitragyna speciosa Korth). Simplisia extracted using solvent (green solvent) choline chloride-sorbitol with a ratio of 1:1; 1:2 and 1:3 g/g with sample and solvent ratio of 1:10 g/mL, microwave strength is 50% watt and performed for 10 minutes, the extract solution has been obtained and then done determination of total levels of polyphenols by using folin ciocalteu reagents and further measured its absorption with the UV-VIS spectrophotometer device at a wavelength of 770 nm. Based on the results of the study, obtained the levels of polyphenols from each comparison of solvent used against the sample of 334 mg of GAE/g samples (1:1 g/g solvent); 372 mg GAE/g samples (1:2 g/g solvents) and 299 mg of GAE/g samples (1:3 g/g solvents). Based on the results of the study can be concluded that the method of choline chloride-sorbitol based microwave assisted extraction (MAE) can be used to extract the polyphenols from Kadamba leaves easily, quickly and efficiently.

scholarly journals Optimization of Microwave-Assisted Extraction of Antioxidants from Bamboo Shoots of Phyllostachys pubescens

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 215 ◽  
Author(s):  
Gualtiero Milani ◽  
Francesca Curci ◽  
Maria Maddalena Cavalluzzi ◽  
Pasquale Crupi ◽  
Isabella Pisano ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Extraction Process ◽  
Biologically Active ◽  
Total Phenolic ◽  
Phyllostachys Pubescens ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Bamboo Shoots ◽  
Assisted Extraction ◽  
Time And Energy

Bamboo is a well-known medicinal plant in Southeast Asia that recently has attracted attention for its high polyphenol content and its medical and nutraceutical applications. In this work, polyphenols have been recovered for the first time by microwave-assisted extraction (MAE) from an unusual Italian cultivar of Phyllostachys pubescens bamboo shoots. The effects of three independent variables, such as extraction time, temperature, and solid/liquid ratio, on polyphenol recovery yield were investigated and successfully optimized through the response surface methodology. We demonstrated that MAE is an excellent polyphenols extraction technique from bamboo shoots because the total phenolic content obtained under microwave irradiation optimal conditions (4 min at 105 °C with 6.25 mg/mL ratio) was about eight-fold higher than that obtained with the conventional extraction method. Furthermore, higher total flavonoid content was also obtained under MAE. Consistent with these results, MAE enhanced the extract antioxidant properties with significant improved DPPH, ABTS, and FRAP scavenging ability. Therefore, this innovative extraction process enhances the recovery of biologically active compounds from Phyllostachys pubescens bamboo shoots with a dramatic reduction of time and energy consumption, which paves the way for its industrial application in functional food production.

scholarly journals Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery (Apium graveolens L.) leaves by response surface methodology

2016 ◽  
Vol 34 (No. 4) ◽  
pp. 341-349 ◽  
Author(s):  
Q. He ◽  
Y. Li ◽  
P. Zhang ◽  
A. Zhang ◽  
H. Wu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Apium Graveolens ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Inhibition Rate ◽  
Microwave Assisted ◽  
Assisted Extraction

The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.

scholarly journals EKSTRAKSI TANIN DARI KULIT KAYU AKASIA DENGAN MENGGUNAKAN MICROWAVE: PENGARUH DAYA MICROWAVE, WAKTU EKSTRAKSI DAN JENIS PELARUT

2017 ◽  
Vol 6 (3) ◽  
pp. 52-57
Author(s):  
Iriany ◽  
Florentina Pandiangan ◽  
Christina Eka P
Keyword(s):  
Atomic Absorption ◽  
Adsorption Capacity ◽  
Microwave Power ◽  
Polar Solvent ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Power Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

Tannin is one of complex polyphenol compound which soluble in polar solvent. Tannin could be extracted from acacia bark. This study aims to examine microwave-assisted extraction of tannins under influence of microwave power, extraction time, and solvent and its application to adsorb Cd and Cu. Tannin was extracted from acacia bark with feed-to-solvent ratio (1:20 g/ml). Power of microwave was adjusted 100 W, 180 W, 300 W, 450 W, and 600 W for 1 minute, 3 minutes and 5 minutes respectively using aquadest and ethanol as the solvent. The extracts were analyzed using UV-Vis spectrophotometer. Furthermore, the extract with the highest yield of tannins are used as the adsorbent. The concentration of Cd and cu were analyzed using Atomic Absorption Spectrophotometer. The result showed the highest yield of tannin 26.606 mg/g at 100 W and 3 minutes extraction time using ethanol as the solvent. The result showed that the adsorption capacity of adsorbent from tannin was 3.81 mg/g and 2.26 mg/g for Cd and Cu respectively.

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