scholarly journals Genetic Variation in Selected Individuals Based on Number of Capsule in M5 Sesame Mutan Lines Detected by RAPD

2019 ◽  
Vol 3 (2) ◽  
pp. 102
Author(s):  
Masruru Kholida ◽  
Taryono Taryono ◽  
Rani Agustina Wulandari
Keyword(s):  
Genetic Variation ◽  
Gamma Rays ◽  
Food Industry ◽  
Rapd Markers ◽  
Mutant Line ◽  
Crop Breeding ◽  
Oilseed Crops ◽  
Research Material ◽  
Mutant Lines ◽  
Individual Line

Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 – 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).  

scholarly journals Detection of changes in growth, yield and genetic variation using RAPD markers among M1V2 and M1V3 generations of irradiated ginger (Zingiber officinale Roscoe)

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 74-82
Author(s):  
Mohd Shari M.A. ◽  
Shamsiah A.
Keyword(s):  
Genetic Variation ◽  
Gamma Rays ◽  
Average Length ◽  
Zingiber Officinale ◽  
Growth And Yield ◽  
Average Weight ◽  
Third Generation ◽  
Zingiber Officinale Roscoe ◽  
Number Of Leaves ◽  
Mutant Lines

The effects of gamma-rays on the growth and yield of twelve mutant lines of Zingiber officinale Roscoe from 2 varieties; namely Bentong and Tanjung Sepat were analysed for a second (M1V2) and a third generation (M1V3). Mutant rhizomes have previously been exposed to different doses (0, 5, 7, 9, 11, and 13 Gy) of gamma-rays that were propagated until the third generation. In the study, the phenotypic characteristic and genetic variation study using Randomly Amplified Polymorphic DNA (RAPD) of ginger mutant lines of M1V2 and M1V3 were analysed and compared with the first generation (M1V1). Parameters such as sprouting rhizome, plant height, the average number of leaves and shoots, average length and width of leaves, and average weights of rhizomes per plant for mutant lines of both varieties were collected. It was observed that the increase in dosage had a negative effect on the growth performance of plants. The number of leaves and shoots, length and width of leaves and average weight of rhizomes for both generations were decreased as the dose increased. The plants from the treatment 5 Gy dose produced the highest ginger rhizome yields of 65.45±1.35 g and 165.0±2.30 g in the Bentong and Tanjung Sepat varieties respectively, after nine months of cultivation in M1V2. While in M1V3, the rhizome yield at 5 Gy showed that the highest rhizome yields were reduced in both varieties of Bentong (45.45±1.25 g) and Tanjung Sepat (125.0±2.30 g). The PCR-based RAPD analysis showed 98.29% of polymorphism which indicated genetic variations between ginger mutant lines. The information provides an important input in determining resourceful management strategies for genetics improvement of ginger.

scholarly journals THE EFFECTS OF GAMMA IRRADIATION AND SOMACLONAL VARIATION ON MORPHOLOGY VARIATION OF MUTANT RODENT TUBER (Typhonium flagelliforme Lodd.) LINES

2015 ◽  
Vol 2 (1) ◽  
pp. 637
Author(s):  
Nesti F. Sianipar ◽  
Ragapadmi Purnamaningsih ◽  
Ireng Darwati ◽  
Danny Laurent ◽  
Chelen .
Keyword(s):  
Genetic Variation ◽  
Gamma Irradiation ◽  
Somatic Cell ◽  
Leaf Area ◽  
Somaclonal Variation ◽  
Plant Height ◽  
Mutant Line ◽  
Leaf Number ◽  
Cell Populations ◽  
Mutant Lines

<p>Rodent tuber (Typhonium flagelliforme Lodd.) is an Indonesian plant with high medicinal potential as anti-cancer. This plant has a low genetic variation in Indonesia. Gamma irradiation can be used to increase genetic variation. This research aimed to explore the effects of gamma irradiation and somaclonal variation on several mutant rodent tuber lines. Six somatic cell populations, which were treated by 6 Gy of gamma irradiation, were successfully regenerated into plantlets. Six mutant lines had been sub cultured into second and third generations by using optimal regeneration media (MS0 supplemented with 0.5 mg/L BAP and 1 mg/L NAA). Rooting of in vitro plantlets had been done by using optimal rooting media MS0 supplemented with 0 mg/L NAA and 0.5 mg/L NAA). Plantlets with good roots were acclimatized and transferred into greenhouse. The morphology of first generation mutant in greenhouse (M1) were characterized and analyzed by using descriptive statistical method. The observed morphological characters including plant height, shoot number, leaf number, and leaf area. A hundred and seventy five (175) mutant lines were obtained on the third generation (MV3) from five somatic cell populations. Mutant line 6-3-x has the highest mean number of shoots, which was 4.62 shoots. Percentage of plant alive after acclimatization in the greenhouse was 78%. Out of 37 M1 mutant lines, morphologically diverse lines were observed with the highest plant height increase: <br />15.5 cm on 6-2-4-1 mutant line, the highest plant shoots increase: 5 shoots on 6-6-7-8 mutant line, the highest leaf number increase: 17 leaves on 6-6-7-8 mutant line, and the highest leaf area increase: 47.24 cm2 on 6-2-5-2 mutant line. In conclusion, gamma irradiation and somaclonal variation could increase genetic variation of mutant rodent tuber lines as shown by morphological data. </p><p><strong>Keywords</strong> : Thyphonium flagelliforme , Gamma-Irradiation Somaclonal-Variation, Morphology-Variation</p>

scholarly journals Mutant Lines of Spring Wheat with Increased Iron, Zinc, and Micronutrients in Grains and Enhanced Bioavailability for Human Health

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Saule Kenzhebayeva ◽  
Alfia Abekova ◽  
Saule Atabayeva ◽  
Gulzira Yernazarova ◽  
Nargul Omirbekova ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Protein Content ◽  
Spring Wheat ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Cereal Products ◽  
Molar Ratios ◽  
Stable Mutant ◽  
Micronutrient Malnutrition ◽  
Mutant Lines

Deficiency of metals, primarily Fe and Zn, affects over half of the world’s population. Human diets dominated by cereal products cause micronutrient malnutrition, which is common in many developing countries where populations depend heavily on staple grain crops such as wheat, maize, and rice. Biofortification is one of the most effective approaches to alleviate malnutrition. Genetically stable mutant spring wheat lines (M7 generation) produced via 100 or 200 Gy gamma treatments to broaden genetic variation for grain nutrients were analyzed for nutritionally important minerals (Ca, Fe, and Zn), their bioavailability, and grain protein content (GPC). Variation was 172.3–883.0 mg/kg for Ca, 40.9–89.0 mg/kg for Fe, and 22.2–89.6 mg/kg for Zn. In mutant lines, among the investigated minerals, the highest increases in concentrations were observed in Fe, Zn, and Ca when compared to the parental cultivar Zhenis. Some mutant lines, mostly in the 100 Gy-derived germplasm, had more than two-fold higher Fe, Zn, and Ca concentrations, lower phytic acid concentration (1.4–2.1-fold), and 6.5–7% higher grain protein content compared to the parent. Variation was detected for the molar ratios of Ca:Phy, Phy:Fe, and Phy:Zn (1.27–10.41, 1.40–5.32, and 1.78–11.78, respectively). The results of this study show how genetic variation generated through radiation can be useful to achieve nutrient biofortification of crops to overcome human malnutrition.

scholarly journals Genetic variability and heritability on Kipas Putih soybean mutant lines using gamma rays irradiation (M3 generation)

2018 ◽  
Vol 122 ◽  
pp. 012041
Author(s):  
Nilahayati ◽  
Rosmayati ◽  
D S Hanafiah ◽  
F Harahap
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Mutant Lines

scholarly journals Detection and analysis of genetic variation in Salicornieae (Chenopodiaceae) using random amplified polymorphic DNA (RAPD) markers

Taxon ◽  
1995 ◽  
Vol 44 (1) ◽  
pp. 53-63 ◽  
Author(s):  
T. Luque ◽  
C. Ruiz ◽  
J. Avalos ◽  
I. L. Calderón ◽  
M. E. Figueroa
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna

Using RAPD markers to identify genetic variation in quackgrass (Elytrigia repens) biotypes

2000 ◽  
Vol 136 (3) ◽  
pp. 253-258
Author(s):  
S MITRA ◽  
P C BHOWMIK ◽  
A IDNURM
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Elytrigia Repens

Genetic Variation in Three Endangered Species of Encholirium (Bromeliaceae) from Cadeia do Espinhaço, Brazil, Setected using RAPD Markers

2006 ◽  
Vol 15 (14) ◽  
pp. 4357-4373 ◽  
Author(s):  
Marcelo Mattos Cavallari ◽  
Rafaela Campostrini Forzza ◽  
Elizabeth Ann Veasey ◽  
Maria Imaculada Zucchi ◽  
Giancarlo Conde Xavier Oliveira
Keyword(s):  
Genetic Variation ◽  
Endangered Species ◽  
Rapd Markers ◽  
Cadeia Do Espinhaço

Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers

1993 ◽  
Vol 2 (3) ◽  
pp. 151-159 ◽  
Author(s):  
I. K. DAWSON ◽  
K. J. CHALMERS ◽  
R. WAUGH ◽  
W. POWELL
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Hordeum Spontaneum

scholarly journals Genetic Variability Studies in M5 Generations of Determinate Early Maturing Cluster Bean [Cyamopsis tetragonoloba (L.) Taub] (MDU-1) Mutants for Yield and Yield Attributing Characters

2022 ◽  
Author(s):  
Devi Suresh ◽  
M. Ananthan ◽  
C. Vanniarajan ◽  
P. Balasubramanian ◽  
T. Sivakumar ◽  
...  
Keyword(s):  
Gamma Rays ◽  
High Yield ◽  
Cyamopsis Tetragonoloba ◽  
Variability Analysis ◽  
Cluster Bean ◽  
Bean Variety ◽  
Early Maturing ◽  
Per Se ◽  
Mutant Lines ◽  
Different Doses

Background: MDU-1 is a high yielding, indeterminate cluster bean variety. In order to develop determinate mutant with high yield, MDU-1 cluster bean variety was irradiated using gamma rays, electron beam and combination of gamma rays and EMS with different doses and the variability induced in M5 generations were studied. Methods: Twenty one best individual plants from M4 generation were selected based on plant height and higher yield and forwarded to M5 generation where they were evaluated in RBD with three replications. Fourteen non-segregating mutant lines with desirable traits were identified in M5 generation and were evaluated for variability along with the parent MDU1. Result: Five best mutant lines namely ACMC-020-04, ACMC-020-11, ACMC-020-08, ACMC-020-10 and ACMC-020-11 were selected from the 14 accessions based on per se performance and variability analysis. The selected mutants needs to be forwarded for stability testing in different environments.

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