scholarly journals Detection of changes in growth, yield and genetic variation using RAPD markers among M1V2 and M1V3 generations of irradiated ginger (Zingiber officinale Roscoe)

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 74-82
Author(s):  
Mohd Shari M.A. ◽  
Shamsiah A.

The effects of gamma-rays on the growth and yield of twelve mutant lines of Zingiber officinale Roscoe from 2 varieties; namely Bentong and Tanjung Sepat were analysed for a second (M1V2) and a third generation (M1V3). Mutant rhizomes have previously been exposed to different doses (0, 5, 7, 9, 11, and 13 Gy) of gamma-rays that were propagated until the third generation. In the study, the phenotypic characteristic and genetic variation study using Randomly Amplified Polymorphic DNA (RAPD) of ginger mutant lines of M1V2 and M1V3 were analysed and compared with the first generation (M1V1). Parameters such as sprouting rhizome, plant height, the average number of leaves and shoots, average length and width of leaves, and average weights of rhizomes per plant for mutant lines of both varieties were collected. It was observed that the increase in dosage had a negative effect on the growth performance of plants. The number of leaves and shoots, length and width of leaves and average weight of rhizomes for both generations were decreased as the dose increased. The plants from the treatment 5 Gy dose produced the highest ginger rhizome yields of 65.45±1.35 g and 165.0±2.30 g in the Bentong and Tanjung Sepat varieties respectively, after nine months of cultivation in M1V2. While in M1V3, the rhizome yield at 5 Gy showed that the highest rhizome yields were reduced in both varieties of Bentong (45.45±1.25 g) and Tanjung Sepat (125.0±2.30 g). The PCR-based RAPD analysis showed 98.29% of polymorphism which indicated genetic variations between ginger mutant lines. The information provides an important input in determining resourceful management strategies for genetics improvement of ginger.

2015 ◽  
Vol 7 (2) ◽  
pp. 964-969 ◽  
Author(s):  
Dileswar Nayak ◽  
N. S. Patil ◽  
L. K. Behera ◽  
D. B. Jadeja

The present investigation was carried out at the Forestry Research Farm, Navsari Agricultural University; Navsari to evaluate the gamma rays at 10 kR, 20 kR and 30 kR induced variability in Jatropha curcas L. on germination, growth and yield for seven Jatropha genotypes (Phule J-1, Urlikanchan, Hansraj, SKN Big, Chhatrapati, Hansot and MPJ-55). The significantly maximum germination percentage (66.96%), seedling survival (74.18%), seedling collar diameter (0.958cm), shoot length (49.442cm), number of leaves per seedling (7.757) and leaf area (37. 58)was observed in Chhatrapati genotype during nursery stage. While low rate of gamma rays treatment (10 kR) had stimulatory effect for germination percentage, seedling survival, seedling collar diameter, shoot length, number of leaves per seedling and leaf area. However, higher gamma rays doses (30 kR) drastically reduced all studied characters. The interaction effect of genotype and gamma rays were significant for number of leaves per seedling. Further, it was not significant in other traits like germination percentage, seedling survival, seedling collar diameter, shoot length and leaf area.


Author(s):  
Eni Sumarni ◽  
Loekas Soesanto ◽  
Noor Farid ◽  
Ardiansyah .

Background: Potato seed production with aeroponic technology can produce a large number of seeds, besides that it is also healthy because it uses plant seeds from tissue culture. However, how the quality of aeroponic seeds produced to become the next seed (growth capacity) has not been studied and scientifically informed. This research aimed to obtain the effect of aeroponic seed yields from the low and high plains based on their size on the growth and yield into advanced seeds. Potato seeds used were from the highland and lowland using aeroponic technology. Methods: This research was conducted in the highland (1000 m above sea level) from April to July 2017. Seeds were classified as S ≤ 1 mg, 1 ≤ Md ≤ 10 g and L ≥ 10 g. This research used a randomized block design with seven repetitions. The tried factors were as follows: 1. The origin of aeroponic seeds (A): A1 (lowland), A2 (highland); 2. The size of aeroponic seeds (U): U1 (S), U2 (M), U3 (L). Plant growth parameters included plant height, number of leaves and number of tubers. Result: The data obtained were analyzed using Duncan’s Multiple Range Test (DMRT) at the 5% level. Seeds of aeroponic yields of various sizes that are planted using drip irrigation have the potential to become advanced seeds. The L-size aeroponic seeds from the lowland produced an average of 5.9 tubers with an average weight of 68.4 g.


2021 ◽  
Vol 8 (1) ◽  
pp. 66-71
Author(s):  
D Jabborova ◽  
Y Enakiev ◽  
Khurshid Sulaymanov ◽  
Dilbar Kadirova ◽  
Ahmad Ali ◽  
...  

Ginger (Zingiber officinale Roscoe) is an important medicinal crop grown for its aromatic rhizome which is used as a spice, food, flavouring agent and medicine. It has been characterised for its hypoglycemic, hypotensive, antioxidant and antibiotic properties. This study was conducted to determine the impact of plant growth-promoting potential of bacterial strain Bacillus subtilis L2 on plant growth and physiological properties of ginger. The experiment was carried out in randomised block design with three replications in pot experiments. The plants were grown in greenhouse conditions for three months. The results showed that at 8 and 12 weeks after planting (WAP) bacterial inoculation increased plant height, leaf length, number of leaves per plant and leaf width. Inoculation with B. subtilis L2 significantly increased plant height by 16, 20 and 18% compared to control at 4, 8 and 12 WAP. At 8 and 12 WAP, leaf length significantly raised by B. subtilis L2 as compared to uninoculated control. B. subtilis L2 significantly increased the number of leaves per plant and leaf width by 30 and 21% respectively when comparing with non-inoculated plants at 8 WAP. The percentage increase in chlorophyll content resulted from the inoculation with B. subtilis L2 over the control was 10.5%, 15.5% and 18.4% at 4, 8 and 12 WAP respectively. It is concluded that there is a significant positive effect of inoculation with B. subtilis L2 on the growth of ginger. B. subtilis L2 strain can be used as a potential agent or bio-fertiliser for stimulation of ginger growth.


2019 ◽  
Vol 3 (2) ◽  
pp. 102
Author(s):  
Masruru Kholida ◽  
Taryono Taryono ◽  
Rani Agustina Wulandari

Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 – 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).  


2010 ◽  
Vol 22 (5) ◽  
pp. 406 ◽  
Author(s):  
Nilesh Pawar ◽  
Sandeep Pai ◽  
Mansingraj Nimbalkar ◽  
Firdose Kolar ◽  
Ghansham Dixit

2019 ◽  
pp. 79-100
Author(s):  
Firas S. Abubaker ◽  
Ali M. Khalifa ◽  
Hasan B. Albaba ◽  
Suliman A. Jadallh

The research was carried out by the Department of Horticulture - Faculty of Agriculture - University of Omar Al-Mukhtar - White during the 2013-2014 and 2014-2015 planting seasons. To study the effect of four planting distances (5, 7.5, 10, 12.5 cm) on the behavior of two varieties of garlic (Egyptian "municipal", Chinese). The design of full randomized splitters was used once in three replicates. The mean of the treatments was measured according to the Duncan test at a significant level of 5%. The results showed that the gradual increase of the planting distance (5, 7.5, 10, 12.5 cm) resulted in a gradual and significant increase in the average length of the leaves, the average number of leaves, the paper area and the dry weight of the leaves in both cultivars. The best results for the leaf content of nitrogen and phosphorus at planting distance were 12.5 cm, while potassium was at a distance of 5 cm. The content of the leaves of chlorophyll (A, B) in both cultivars increased by increasing the distance of cultivation with the superiority of the Chinese class on the Egyptian. The results showed that increasing the planting distance to 12.5 cm increased the average weight of the bulb, the exportable yield and the exportable yield of the total crop, while the total yield decreased with the increase of the planting distance. The highest percentage of nitrogen was recorded at a distance of 12.5 cm for the Egyptian variety, while the Chinese category recorded the highest percentage of phosphorus and potassium. The results showed no significant differences in mean weight of the lobes between the four planting distances of the Egyptian and Chinese varieties during the first and second seasons. The best results were achieved for total dissolved solids at a distance of 12.5 cm in the Chinese and Egyptian varieties, respectively.


Author(s):  
Эльвира Алексеевна Алексеева ◽  
О. Г. Димитров ◽  
Л. Н. Шантанова ◽  
С. М. Николаев ◽  
Е. Н. Карева ◽  
...  

Изучены адаптогенные свойства растительного средства «Кардекаим», представляющего собой сухой экстракт из сырья Inula helenium L., Zingiber officinale Roscoe, Elletaria cardamomum (L.) Maton., Caragana spinosa (L.) Wall. ex Hornem. и содержащего в качестве действующих веществ фенольные соединения и терпеноиды. Курсовое превентивное введение кардекаима в дозе 100 мг/кг в течение 7 дней перед острым эмоциональным стрессом оказывает выраженное стресс-протективное действие, препятствуя развитию признаков «триады Селье»: инволюции вилочковой железы (40 %), язвенным повреждениям слизистой оболочки желудка (в 5 раз) и в меньшей степени — гипертрофии надпочечников (p ≤ 0,05), полученный эффект не уступает препарату сравнения — экстракту элеутерококка. Показано, что повышение резистентности к стрессу под влиянием кардекаима сопровождается активацией синтеза белков теплового шока (Hsp-70), не связанной со стресс-индуцированной генерацией NO.


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