Performa Produksi dan Analisis Usaha Puyuh (Coturnix coturnix japonica)  yang diberi Substitusi Black Soldier Fly Larvae (BSFL) pada Pakan Komersil

Black Soldier Fly Larvae (BSFL) is an alternative protein source for livestock. This research aimed to know the effectiveness of BSFL for commercial feed substitution, related to production, productivity, and profitability in quail farm. This study used 80 quails which divided into 4 groups and each group consist of 20 quails. The treatments contained BSFL substitution with different composition, control group (P0) was given 100% commercial feed, (P1) was given a 5% BSFL substitution, (P2) was given a 10% BSFL substitution and (P3) was given a 20% BSFL substitution. Contribution margin (CM) analysis was used to determine the yields of the groups in this study. Results showed that the highest feed consumption rate is in P3, production (egg in total and weight), productivity and yields in P3 is the highest, although there's no difference between P0, P2 and P3 based on the analysis MANOVA. BSFL substitution at 20% is recommended to the farmers to increase profits.

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Feeding live Black Soldier Fly larvae (Hermetia illucens) to laying hens: effects on feed consumption, hen health, hen behaviour and egg quality

Poultry Science ◽

10.1016/j.psj.2021.101400 ◽

2021 ◽

pp. 101400

Author(s):

Fernanda M. Tahamtani ◽

Emma Ivarsson ◽

Viktoria Wiklicky ◽

Cecilia Lalander ◽

Helena Wall ◽

...

Keyword(s):

Laying Hens ◽

Egg Quality ◽

Feed Consumption ◽

Black Soldier Fly ◽

Hermetia Illucens ◽

Black Soldier Fly Larvae ◽

Fly Larvae

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Enrichment of polyunsaturated fatty acids in black soldier fly larvae (Hermetia illucens) fortified with squid liver oil

Journal of Insects as Food and Feed ◽

10.3920/jiff2021.0030 ◽

2021 ◽

pp. 1-12

Author(s):

G. Tirtawijaya ◽

J.-S. Choi

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Animal Feed ◽

Control Group ◽

Feed Conversion ◽

Black Soldier Fly ◽

Hermetia Illucens ◽

Growth Increase ◽

Black Soldier Fly Larvae ◽

Fly Larvae

Black soldier fly larvae (BSFL; Hermetia illucens) are known as an alternative feed for livestock, but their lack of polyunsaturated fatty acids (such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) makes it less valuable. To overcome these problems, BSFL substrate (chicken feed) was fortified with squid liver oil (SLO) at five different concentrations (0, 2.5, 5, 10, and 20%). The growth rate, feed conversion, and nutritional content of BSFL were evaluated at day 15 of rearing. Of the five concentrations, SLO 5% showed the highest growth increase (25.82-fold) among the other treatments (20.63-22.98-fold; P<0.05). The fortification of SLO 5% did not result in differences in survival and feed conversion of larvae compared to larvae fed the control substrate. By rearing BSFL in a substrate containing SLO 5%, the lipid content of the larvae was 32% higher than that of larvae fed the control substrate (P<0.05). The accumulation of lipids was faster in the substrate containing SLO 5% (33.20% for 8 days) than in the control substrate (24.36% for 15 days). The control group of BSFL contained no DHA or EPA, but after rearing on the SLO 5% fortified substrate, the larvae contained DHA at an average level of 2.99 g/100 g lipid and EPA was 2.68 g/100 g lipid. Harvested larvae from SLO 5% treatment was within safe levels of Pb, Cd, As, and Hg (840, 370, 860, and 26.7 μg/kg, respectively), under the EU threshold for animal feed. Based on our results, it concluded that BSFL enriched with PUFAs, DHA and EPA can be considered as important nutritional components of animal feed without excessive heavy metals accumulation by feeding SLO in an appropriate amount.

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Nutrient and AA digestibility of black soldier fly larvae differing in age using the precision-fed cecectomized rooster assay1

Journal of Animal Science ◽

10.1093/jas/skz363 ◽

2019 ◽

Vol 98 (1) ◽

Cited By ~ 4

Author(s):

Sungho Do ◽

Liz Koutsos ◽

Pamela L Utterback ◽

Carl M Parsons ◽

Maria R C de Godoy ◽

...

Keyword(s):

Protein Quality ◽

Black Soldier Fly ◽

Feed Withdrawal ◽

Randomized Design ◽

Alternative Protein ◽

High Quality Protein ◽

Black Soldier Fly Larvae ◽

Fly Larvae ◽

Nutrient Profiles ◽

The University

Abstract Edible insects such as black soldier fly larvae (BSFL) are alternative protein sources for animal feeds due to their high-protein content and potential low environmental footprint. However, protein quality and AA content may vary across insect species and age. Our objective was to determine the effects of age on nutrient and AA digestibility of BSFL intended for use in pet foods using the precision-fed cecectomized rooster assay. All animal procedures were approved by the University of Illinois Institutional Animal Care and Use Committee prior to experimentation. Twenty-four cecectomized roosters (four roosters per substrate) were randomly assigned to test substrates [BSFL0 = day 0 (day of hatch); BSFL11 = day 11; BSFL14 = day 14; BSFL18 = day 18; BSFL23 = day 23; BSFL29 = day 29]. After 24 h of feed withdrawal, roosters were tube-fed 20 g of test substrates. Following crop intubation, excreta were collected for 48 h. Endogenous corrections for AA were made using five additional cecectomized roosters. All data were analyzed using a completely randomized design and the GLM procedure of SAS 9.4. DM and OM digestibilities were not different among substrates, but acid-hydrolyzed fat digestibility tended to be greater (P < 0.10) for BSFL23 and BSFL29 than BSFL14 and BSFL18. Although all substrates had a high digestibility, BSFL0 and BSFL11 had the lowest (P < 0.05) digestibilities for most indispensable and dispensable AA. Digestible indispensable AA score (DIAAS)-like values were calculated to determine protein quality according to AAFCO nutrient profiles and NRC recommended allowances for dogs and cats. In general, BSFL18 had the highest, and BSFL11 had the lowest DIAAS-like values for most indispensable AA. Threonine, methionine, and tryptophan were often the first-limiting AA. Our results suggest that BSFL are a high-quality protein and AA source, but that age can affect the AA digestibility and protein quality of this alternative protein source.

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Substitution of fish meal with black soldier fly larvae (Hermetia illucens) meal to eggs production and physical quality of quail (Coturnix coturnix japonica) eggs

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/492/1/012014 ◽

2020 ◽

Vol 492 ◽

pp. 012014

Author(s):

Suparman ◽

S Purwanti ◽

N Nahariah

Keyword(s):

Fish Meal ◽

Coturnix Japonica ◽

Coturnix Coturnix Japonica ◽

Black Soldier Fly ◽

Hermetia Illucens ◽

Physical Quality ◽

Coturnix Coturnix ◽

Black Soldier Fly Larvae ◽

Fly Larvae

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228 Standardized Ileal Digestibility of Partially Defatted Black Soldier Fly Larvae Meal in Growing Pig Diets

Journal of Animal Science ◽

10.1093/jas/skab235.221 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 120-121

Author(s):

Fiona Tansil ◽

Lee-Anne Huber ◽

Elijah Kiarie ◽

Daniel A Columbus ◽

Anna-Kate Shoveller

Keyword(s):

Fish Meal ◽

Latin Square ◽

Black Soldier Fly ◽

Promising Alternative ◽

Ileal Digestibility ◽

Growing Pig ◽

Alternative Protein ◽

Black Soldier Fly Larvae ◽

Fly Larvae

Abstract New ingredients need to be characterized both chemically and in vivo, then compared to commonly-used protein ingredients before they can be considered for use in feeds. The objective of the present study was to determine the standardized ileal digestibility (SID, %) of amino acids (AA) of partially defatted black soldier fly larvae meal (BSFLM) (49.7% CP; 14.4% crude fat as fed). Subsequently, these results were compared to the SID AA of common protein ingredients in swine feed, fish meal (FM) and soybean meal (SBM) (NRC 2012), and to published full-fat (FF) BSFLM SID values. Six ileal-cannulated barrows (18.03 ± 0.67 kg BW) were used in a 2x2 Latin square design and fed either a nitrogen-free diet (NFD) or BSFLM-containing diet over two 11-d experimental periods. In each period, barrows were adapted for 7 days to the diet and ileal digesta collection was conducted on day 10 and 11 for 8h per day. Basal endogenous AA losses (EAAL) were calculated from pigs fed the NFD and as expected, the ileal AA content were significantly higher for pigs fed the BSFLM diet than NFD (P < 0.05). SID for AA were then calculated based on the obtained EAAL values following Stein et al., 2007. For essential AA, the SID of BSFLM were all above 82%, the highest being Arg (93.76%), followed by Met (91.32%), and Phe (89.80%). The SID of essential AA were on average 5.0% higher in BSFLM than FM, but 2.7% lower than SBM. When compared to the FF BSFLM, SID of essential AA of BSFLM were 1–5% higher, except for Phe and Thr. These results suggest that partially defatted BSFLM is a promising alternative protein ingredient in growing pig diets, which has a more digestible AA profile than FM, only slightly lower than SBM, and comparable to the FF BSFLM.

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A Molecular Survey of Bacterial Species in the Guts of Black Soldier Fly Larvae (Hermetia illucens) Reared on Two Urban Organic Waste Streams in Kenya

Frontiers in Microbiology ◽

10.3389/fmicb.2021.687103 ◽

2021 ◽

Vol 12 ◽

Author(s):

Marwa Shumo ◽

Fathiya M. Khamis ◽

Fidelis Levi Ombura ◽

Chrysantus M. Tanga ◽

Komi K. M. Fiaboe ◽

...

Keyword(s):

Animal Feed ◽

Bacterial Species ◽

Chicken Manure ◽

Black Soldier Fly ◽

Dependent Sequence ◽

Alternative Protein ◽

Black Soldier Fly Larvae ◽

Fly Larvae ◽

Rdna Amplification ◽

Culture Dependent

Globally, the expansion of livestock and fisheries production is severely constrained due to the increasing costs and ecological footprint of feed constituents. The utilization of black soldier fly (BSF) as an alternative protein ingredient to fishmeal and soybean in animal feed has been widely documented. The black soldier fly larvae (BSFL) used are known to voraciously feed and grow in contaminated organic wastes. Thus, several concerns about their safety for inclusion into animal feed remain largely unaddressed. This study evaluated both culture-dependent sequence-based and 16S rDNA amplification analysis to isolate and identify bacterial species associated with BSFL fed on chicken manure (CM) and kitchen waste (KW). The bacteria species from the CM and KW were also isolated and investigated. Results from the culture-dependent isolation strategies revealed that Providencia sp. was the most dominant bacterial species detected from the guts of BSFL reared on CM and KW. Morganella sp. and Brevibacterium sp. were detected in CM, while Staphylococcus sp. and Bordetella sp. were specific to KW. However, metagenomic studies showed that Providencia and Bordetella were the dominant genera observed in BSFL gut and processed waste substrates. Pseudomonas and Comamonas were recorded in the raw waste substrates. The diversity of bacterial genera recorded from the fresh rearing substrates was significantly higher compared to the diversity observed in the gut of the BSFL and BSF frass (leftovers of the rearing substrates). These findings demonstrate that the presence and abundance of microbiota in BSFL and their associated waste vary considerably. However, the presence of clinically pathogenic strains of bacteria in the gut of BSFL fed both substrates highlight the biosafety risk of potential vertical transmission that might occur, if appropriate pre-and-postharvest measures are not enforced.

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