scholarly journals Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai)

2021 ◽  
Vol 6 (3) ◽  
pp. 61858
Author(s):  
Andra Jausa Salsabila ◽  
Budi Setiadi Daryono ◽  
Aprilia Sufi Subiastuti

Indonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed by making triploid orange watermelons. The new watermelon cultivar (F₁) is the result of crossing 'Jelita' (♀) and 'BallyBall' (♂) which are expected to provide a combination of characters that can produce large and sweet fruits. The purpose of this study was to identify ISSR markers that were associated with ploidy levels of horticultural crops, particularly watermelon. The watermelons were growns on the agricultural land of Kebondalem Hamlet, Madurejo Village, Prambanan District, D.I.Y. and analyzed at the Laboratory of Biotechnology PAU, UGM and Laboratory of Genetics and Breeding, Faculty of Biology UGM. Analysis of genetic variation was carried out using the PCR-ISSR method and the DNA bandwidth was calculated using Paint Apps, Microsoft Excel 2013, and Microsoft Word 2013. The results of crossing 'Jelita' and 'King Quality 'is sterile. The result of 'Jelita' with 'BallyBall' produces tillers until sterile harvest time. The results of the analysis of genetic variation using the PCR-ISSR method indicate that the ISSR BI, B3, B5, and CBTC 1 molecular markers cannot be used to distinguish ploidy from the tested watermelons. 

Author(s):  
Meysam Madadi ◽  
Zabihollah Zamani ◽  
Reza Fatahi

Pomegranate is one of the most important horticultural crops in Iran, and has been cultivated for thousands of years in this country. At this period due to selection of superior cultivars from nature or mutation emerged in these cultivars, and their vegetative propagation, substantial genetic variation has occurred within and among the cultivars. Thus, each cultivar may consist of different clones. According to this issue, diversity within four commercial cultivars of pomegranate was analyzed. Two molecular marker systems including ISSR and SSR were used to evaluate variability between 36 samples of four commercial cultivars. ISSR markers produced 114 amplification products, out of which 97 were polymorphic (83.23%). Mean resolving power was 2.96 for ISSR markers. 19 SSR molecular markers were used, 15 of which amplified polymorphic products, while the remaining ones monomorphic., The number of polymorphic alleles per locus ranged from two to four (average 3.6). The observed and expected heterozygosities ranged from 0.04 to 0.92 and 0.14 to 0.62, respectively. In addition, mean polymorphic information content was 0.45 for SSR loci. Our results showed that commercial Iranian pomegranate have different clones. Therefore, ISSR and SSR markers can be a useful tools for detecting clones of each cultivar.


1986 ◽  
Vol 25 (4) ◽  
pp. 839-853
Author(s):  
Sarfraz Khan Qureshi

Taxation of the agricultural sector is a major instrument for mobilization of the surplus to finance development projects within the agricultural sector and/or the rest of the economy. For many years, the need for a heavier taxation of agricultural land has formed part of the conventional wisdom regarding the ways of extracting agricultural surplus and increasing the tempo of agricultural development in poor countries. Land taxes have both equity and efficiency properties that gladden the hearts of both economists and vocal politicians belonging to urban areas. Taxes on land promote efficiency in the allocation of scarce resources by creating incentives for farmers to increase their effort and reduce their consumption, thus expanding the amount of agricultural produce available to the non-agricultural sectors of the economy. A tax on land has an important redistributive function because its incidence falls squarely on the landlord and is shifted neither forward to consumers nor backwards to suppliers of agricultural inputs; nor does it introduce distortions in the allocation of productive resources.


2020 ◽  
Author(s):  
Hanny Puput Eliyarista Saragih

Perangkat lunak (software) adalah istilah khusus untuk data yang diformat, dan disimpan secara digital. Perangkat lunak tersusun atas program yang menentukan apa yang harus dilakukan oleh komputer. Komputer harus melaksanakan instruksi yang terdapat dalam program untuk mendapatkan hasil yang berguna. Ada dua macam perangkat lunak yaitu sistem software dan application software. Sistem software berguna untuk mengatur file, me-load dan mengeksekusi program serta menerima perintah dari mouse atau keyboard. Sistem software biasa disebut dengan sistem operasi (operating system). Sedang application software adalah software yang digunakan untuk menyelesaikan suatu pekerjaan. Contoh application software adalah Microsoft Word, Microsoft Excel.


2020 ◽  
Author(s):  
Hanny Puput Eliyarista Saragih

Perangkat lunak (software) adalah istilah khusus untuk data yang diformat, dan disimpan secara digital. Perangkat lunak tersusun atas program yang menentukan apa yang harus dilakukan oleh komputer. Komputer harus melaksanakan instruksi yang terdapat dalam program untuk mendapatkan hasil yang berguna. Ada dua macam perangkat lunak yaitu sistem software dan application software. Sistem software berguna untuk mengatur file, me-load dan mengeksekusi program serta menerima perintah dari mouse atau keyboard. Sistem software biasa disebut dengan sistem operasi (operating system). Sedang application software adalah software yang digunakan untuk menyelesaikan suatu pekerjaan. Contoh application software adalah Microsoft Word, Microsoft Excel


2018 ◽  
Vol 16 (1) ◽  
pp. 103-117
Author(s):  
Nurul Istiqomah ◽  
Nunung Sri Mulyani ◽  
Izza Mafruhah ◽  
Dewi Ismoyowati

Indonesia as an agricultural country has the potential to compete in the agricultural market in the international market, in line with the existence of the ASEAN / ASEAN Free Trade Area (AFTA) Free Market. Ngawi Regency is a fertile area and is one of the buffer zones of the agricultural sector in East Java. Horticulture commodities are one of the main sources in the agricultural sector, because they have high potential and can contribute to the economy of a region. Horticultural commodities in the form of fruits and vegetables are an important food source to meet the nutritional needs of the community. Agriculture with a focus on horticultural crops in Ngawi Regency was developed with a cluster system based on the level of progress, harvest area and by considering agro-climate to map the superior horticultural commodities. The purpose of this study was to map the conditions of horticultural agriculture and analyze problems in the cluster of horticulture plants in Ngawi Regency. The research method is a mixed method using descriptive analysis, Geographic Information System (GIS), and using the Analysis Hierarchy Process (AHP). The conclusion of this study is that the potential development of horticultural clusters in Ngawi Regency requires structuring and developing the location of base commodities in accordance with the conditions of the agro-ecosystem. The development of existing commodities at these base points will make the commodity superior and support the creation of horticultural cluster centers and the development of existing agribusiness in an area. Development of horticulture base commodities for seasonal vegetables and fruits can be adjusted to the LQ results for each sub-district in Ngawi Regency. The results of the Indepth interview processed using AHP obtained results that in fact there were three main factors in the development of clusters, namely production consisting of four derivative factors namely research and development, superior seeds, fertilizers and anti-pest drugs and then marketing with derivative factors namely product standardization, packaging , traditional markets and modern markets. Then the third factor of the institution consists of training, networking, government support and assistance. 


2012 ◽  
Vol 50 (No. 11) ◽  
pp. 486-494
Author(s):  
Z. Chrastinová

In the year before the accession to the European Union, the Slovak agricultural sector reported a loss of SKK 2.4 billion and following a profitable year, the earnings were reduced by SKK 2.8 billion. The situation was caused by a number of reasons, namely reduced sales of agricultural products, damage resulting from adverse weather effects (cold weather, hail, drought and  swine fever), as well as widening of the price gap compared to the year before (increasing input prices in agriculture and decreasing purchase prices of agricultural products, especially in livestock production). Legal entities and natural persons experienced mixed business success. While 51% of legal entities made profit, the figure rose to 76% in the group of natural persons. Both the agricultural cooperatives and trading companies performed with a loss. The loss per hectare of agricultural land (a.l.) was substantially lower in the case of business companies. Natural persons - private farmers were profitable over the period. The gap between the profitable and loss-making enterprises has widened. Some 60% of profitable enterprises owned by legal entities made only a small profit below SKK 0.5 million. The loss-making performance was typical for more productive areas of Slovakia. This was related to stronger effects of adverse climate in 2003.


2020 ◽  
Vol 5 (1) ◽  
pp. 817-825
Author(s):  
Susanna L. Middelberg ◽  
Pieter van der Zwan ◽  
Cobus Oberholster

AbstractThe Zambian government has introduced the farm block development programme (FBDP) to facilitate agricultural land and rural development and encourage private sector investment. This study assessed whether the FBDP achieves these goals. Key obstacles and possible opportunities were also identified and, where appropriate, specific corrective actions were recommended. Qualitative data were collected through semi-structured interviews conducted in Lusaka with various stakeholders of the FBDP. The FBDP is designed to facilitate agricultural land development and encourage private sector investment. However, the programme falls far short in terms of implementation, amidst policy uncertainty and lack of support. This is evident by the insecurity of land tenure which negatively affects small- and medium-scale producers’ access to financing, lack of infrastructure development of these farm blocks, and constraints in the agricultural sector such as low labour productivity and poor access to service expertise. It is recommended that innovative policy interventions should be created to support agricultural development. This can be achieved by following a multistakeholder approach through involving private, public and non-profit sectors such as non-governmental organisations (NGOs) and donors.


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