scholarly journals Determination of Total Xanthone Content in the Preparation of Mangosteen Pericarp Capsules (garcinia mangostana l.) Available on the Market using UV-Visible Spectrophotometry Method

2019 ◽  
Vol 24 (2) ◽  
pp. 98 ◽  
Author(s):  
Delladari Mayefis ◽  
Yandri Anugerah ◽  
Roslinda Rasyid
Keyword(s):  
Limit Of Detection ◽  
Garcinia Mangostana ◽  
Visible Spectrophotometry ◽  
A Value ◽  
Limit Of Quantitation ◽  
Uv Visible ◽  
Market Needs ◽  
Interday Precision ◽  
Maximum Absorption Wavelength

The skin of Mangosteen fruit (Garcinia mangostana L.) is widely used as traditional medicine, for it contains a lot of xanthone compounds. Today, there are many products from mangosteen pericarp extract being sold on the market, one of which is the preparation of mangosteen pericarp capsules. Determining the total xanthone content in the preparation of mangosteen pericarp capsules (Garcinia mangostana L.) circulating on the market needs to be done to calculate the levels of xanthone that are not included in the preparation label. The results of the analysis with UV-Visible Spectrophotometry obtains the maximum absorption wavelength at 243 nm and the linear regression equation of the calibration curve that is y=0,0568+0,0727x with a value of r=0,9999. Validation of the analysis method showed the results of intraday precision obtained % RSD 0,34%, 0,17%, and 0,16%, interday precision of 0,23%, 0,35%, and 0,16%. The accuracy obtained was mean % recovery 95,55%, limit of detection 0,35 μg/ml and limit of quantitation 1,15 μg/ml. The results of the determination of the total xanthone content in the mangosteen pericarp capsules were 100,8 μg/mg in sample A; 197 μg/mg in sample B; and 50,2 μg/mg in sample C.

scholarly journals Verifikasi Metode Penentuan Residu Pestisida Beta Siflutrin dalam Kentang (Solanum tuberosum L) secara Kromatografi Gas (Verification of Method for Determination of Residual Pesticides Beta Siflutrin in Potato (Solanum tuberosum L) with Gas Chromatography)

1970 ◽  
Vol 3 (1) ◽  
pp. 50-55
Author(s):  
Fahrizal Hazra ◽  
Lisa Rosdiana
Keyword(s):  
Gas Chromatography ◽  
Solanum Tuberosum ◽  
Limit Of Detection ◽  
Solanum Tuberosum L ◽  
Nitrogen Gas ◽  
Verification Method ◽  
A Value ◽  
Relative Standard ◽  
Limit Of Quantitation

The determination of residual pesticides beta siflutrin in potato (Solanum tuberosum L) was conducted by Gas Chromatography (GC). The separation was conducted on a DB-1 (30m×0.25mm×0.25µm) coloum, using a nitrogen gas as mobile phase at flow rate of 25 mL/min. The determination of beta siflutrin in potato with GC was based on the verification method. The detection was ECD detector. Verification parameters tested were linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ). Linearity had a correlation coefficient (r) of 0.9992. Accuracy had a recovery value of 99.49%. Precision had a value of relative standard deviation (% RSD) of 2.48%. The limit of detection (LOD) and limit of quantitation (LOQ) obtained was 0.001305 and 0.00435 mg/kg. The result of this research showed that the method of determination of residual pesticides beta siflutrin in potato with GC can be used as a routine analysis.Key words: verification, beta siflutrin, GC, potato

scholarly journals DETERMINATION OF Fe (II) IN KATUK LEAF (Saoropusandrogynus) CAPSULES USING UV-Vis SPECTROPHOTOMETER METHOD

2019 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Tisa Mandala Sari
Keyword(s):  
Iron Content ◽  
Limit Of Detection ◽  
Analysis Method ◽  
Validation Criteria ◽  
Limit Of Quantitation ◽  
Uv Visible ◽  
R Value ◽  
Ion Analysis

Katuk leaf capsules generally use as ASI emitters and katuk leaf capsules also contain iron (II) which to prevent aneemia. Reseaerch on the determination of iron content in katuk leaf capsulse with UV-Visible spectrophotometricmethod has been done. The principle of this research is leuched dry katuk leaf capsulse with HCl 6 M. Then performed the determination rate of iron at 510 nm wavelength by way of reacting iron (II) with 1,10-phenantrolin. So that the complex is formed ferroin orange red. Validation of iron (II) ion analysis method in katuk leaf capsulse has fulfilled the validation criteria that is liniearity with r value = 0,9986, limit of detection (LOD) 0,3588µg/mL, limit of quantitation (LOQ) 1,1960µg/mL, iron (II) content in the sample SI 0,0153µg/mL, SII 0,0225µg/mL, and SIII 0,0268µg/mL. 

scholarly journals Validation of an HPLC Method for Determination of Pentoxifylline in Human Plasma and Its Application to Pharmacokinetic Study

2009 ◽  
Vol 92 (3) ◽  
pp. 837-845 ◽  
Author(s):  
Pattana Sripalakit ◽  
Aurasorn Saraphanchotiwitthaya
Keyword(s):  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Internal Standard ◽  
Hplc Method ◽  
Dose Administration ◽  
Limit Of Quantitation ◽  
Interday Precision

Abstract An HPLC method suitable for routine determination of pentoxifylline in human plasma has been adapted and validated. Sample preparation was done by solid-phase extraction. Chloramphenicol was used as the internal standard. The linear range was from 15400 ng/mL (r2 = 0.9994), with a limit of quantitation of 15 ng/mL. The limit of detection was found to be 5 ng/mL. The intra- and interday accuracy ranged from 98.0 to 110.2 and the coefficient of variation was not more than 8.8 for both intra- and interday precision. The absolute recoveries of pentoxifylline and chloramphenicol from human plasma were >97. The method was validated with excellent specificity, accuracy, precision, recovery, and stability. The pharmacokinetic study of a generic pentoxifylline 400 mg tablet in healthy Thai male volunteers after a single dose administration was determined by this developed assay.

scholarly journals Preconcentration and determination of Metformine Hydrochloride in different samples by cloud point extraction coupling with uv-visible spectrophotometry

2016 ◽  
Vol 13 (2) ◽  
pp. 470-479
Author(s):  
Baghdad Science Journal
Keyword(s):  
Cloud Point ◽  
Calibration Curve ◽  
Cloud Point Extraction ◽  
Limit Of Detection ◽  
Molar Absorptivity ◽  
Basic Medium ◽  
Optimum Conditions ◽  
Visible Spectrophotometry ◽  
Uv Visible

This work was influenced the separation and preconcentration steps were carried out to determination of metformin (MET) in pharmaceutical preparations and human serum samples. Complex formation method and cloud-point extraction (CPE) coupling with UV-Visible spectrophotometry were used to investigated of study target.The results has showed the best optical characteristic for calibration curve and statistical data which were obtained under optimum conditions. The first method is based on the reaction of MET with nickel (II) in alkaline medium an absorption maximum ?)max) at 434nm. ''Beer's low'' is obeyed in the concentration range (10-100µg.ml-1) with molar absorptivity of 3.9x103 L.mol-1.cm-1.The limit of detection and quantitation values were 2.37 and7.11 µg.ml-1 respectively. The second method based on extraction of traces amounts of MET using the cloud-point extraction (CPE). This method implicated for using of a nonionic surfactant (Triton x-114) as an extraction medium which was entrap the hydrophobic complex formed between MET and nickel(ii) in basic medium as reaction system for designing the CPE procedure. The optimum conditions were similar the first method expect the amount of surfactant which was 0.5 ml. The concentrations range of calibration curve from 3.5to100 µg.ml-1 and molar absorptivity of 1.2x104 L.mol-1.cm-1. In this method was access to less of concentrations in Limit of detection and quantitation which were 0.74and 2.22 µg.ml-1 respectively. The precise (RSD %) and accuracy (recovery %) of both methods were ranged between 0.24-0.47, 97.86-98.68 respectively. The data of two methods were appeared high acceptable with standered of British Pharmacopoeia through using statistic methods (f-test and t-test), that they may be used in analysis of MET.

scholarly journals Validasi Metode Spektrofotometri Visible Untuk Penentuan Kadar Formaldehida Pada Pembalut Wanita Yang Beredar Di Pasaran

2017 ◽  
Vol 2 (1) ◽  
pp. 9-16
Author(s):  
Cicik Herlina Yulianti ◽  
Vika Ayu Devianti ◽  
M.A. Hanny Ferry Fernanda
Keyword(s):  
Limit Of Detection ◽  
Visible Spectrophotometry ◽  
Accuracy And Precision ◽  
Validation Criteria ◽  
Limit Of Quantitation ◽  
The Government ◽  
Nash Reagent ◽  
Positive Results ◽  
Specific Reagent

ABSTRAKPembalut menjadi kebutuhan wanita yang sangat penting karena digunakan untuk menyerap cairan darah ketika mengalami menstruasi. Pada pembuatan pembalut wanita dimungkinkan adanya pemakaian formaldehida. Oleh karena itu, pembalut wanita termasuk salah satu alat kesehatan yang kandungan dan bahan penyusunnya diatur oleh pemerintah. Pada penelitian ini menggunakan metode spektrofotometri visibel untuk penentuan kadar formaldehida dalam pembalut wanita sekali pakai. Sebelum digunakan, maka metode spektrofotometri visibel ini harus divalidasi terlebih dahulu untuk memastikan bahwa metode spektrofotometri yang digunakan dapat memberikan hasil yang akurat. Tujuan penelitian ini adalah melakukan validasi metode spektrofotometri visibel untuk penetapan kadar formaldehida dalam pembalut wanita sekali pakai menggunakan pereaksi nash sebagai reagen spesifik. Metode penelitian yang digunakan adalah pembuatan dan pembakuan larutan baku formaldehida, menentukan panjang gelombang maksimal, pembuatan kurva kalibrasi, melakukan uji linieritas, uji LOD dan LOQ, serta uji kesesuaian dan kecermatan, dan menentukan kadar formaldehida pada pembalut wanita. Hasil dari penelitian ini adalah bahwa metode spektrofotometri visibel memiliki selektifitas, linieritas,batas deteksi dan kuantitasi, presisi dan akurasi yang baik. Kadar rata-rata formaldehida pada ke lima sampel pembalut sebesar 2,88 mg/kg - 4,05 mg/kg.Kata kunci: pembalut, formaldehida, validasi, spektrofotometri visibelABSTRACTSanitary napkins are a very important woman’s need to absorb blood fluids when menstruating. In the manufacture of sanitary napkins may contain formaldehyde additives. Therefore, sanitary napkins are one of the medical devices whose composition is regulated by the government. In this study to identify the use of formaldehyde in sanitary napkins was carried out by visible spectrophotometry using nash reagent. This method should be validated in advance to ensure that the method used can provide accurate data. The aim of this research is to validate visible spectrophotometry method for determination of formaldehyde content indisposable sanitary napkins using nash reagent as specific reagent. Validation of UV – Vis spectrophotometry method for determination of formaldehyde showed that Nash reagent was suitable to determine formaldehyde. This method is linear with correlation coefficient (r2) of 0,99967. The validation characteristics include accuracy and precision, linearity, limit of detection, and limit of quantitation. The acceptance validation criteria were found in all case. Qualitative determination in five sanitary napkins samples showed positive results and the quantitative analysis confirmed that the average content of formaldehyde in five sanitary napkins samples was 2,88 mg/kg – 4,05 mg/kg.Keywords: sanitary napkins, formaldehyde, validation, visible spectrophotometry

Simultaneous Determination of Four Herbicide and Pesticide Residues in Chinese Green Tea Using QuEChERS Purification Procedure and UPLC-MS/MS Analysis

2013 ◽  
Vol 634-638 ◽  
pp. 1586-1590
Author(s):  
Su Fang Wang ◽  
Shou Jie Zhang ◽  
Chun Hong Dong ◽  
Guo Qing Wang ◽  
Jun Feng Guo ◽  
...  
Keyword(s):  
Formic Acid ◽  
Simultaneous Determination ◽  
Green Tea ◽  
Limit Of Detection ◽  
Multiple Reaction Monitoring ◽  
Graphitized Carbon Black ◽  
Ms Analysis ◽  
Relative Standard ◽  
Limit Of Quantitation

A method for simultaneous determination of residuals of four herbicides and pesticides, simazine, carboxin, diflubenzuron and rotenone, in Chinese green tea was developed. In the proposed method, the tea powder was placed in a centrifuge tube with a plug, extracted in saturated aqueous sodium chloride solution and acetonitrile, agitated using vortex oscillator, and then centrifuged 5 min at 4000 rpm. The supernatant solution was purified by primary secondary amine (PSA) sorbent, C18 power, and graphitized carbon black powder, respectively. Then the purified extracts were dissolved with acetonitrile:0.1% formic acid aqueous solution (40:60, V/V) and agitated, filtered using a syringe with 0.22 μm nylon filter prior to UPLC-MS/MS analysis. The UPLC analysis was performed on an ACQUITY UPLC® HSS T3 column (2.1 mm×100 mm, 1.8 µm), using acetonitrile-0.1% formic acid as mobile phase with the flow rate as 0.3 mL•min-1. Injection volume was 10 µL. Positive ionization mode was applied, and the ions were monitored in the multiple reaction monitoring (MRM) mode with curtain gas 0.069 MPa, collision gas 0.052 MPa, ESI ion spray voltage 5000 V, temperature 550 °C, nebulizer gas 0.24 MPa, and turbo gas 0.28 MPa. The limit of detection (LOD) and limit of quantitation (LOQ) of the proposed method are 1 μg•kg-1and 5 μg•kg-1, respectively. The average recoveries of the four pesticides at 10, 20, and 50 µg•kg-1spiking levels range from 77.4% to 95.3%. TheSupersSuperscript textcript textrelative standard deviation (RSD) (n=6) range form 11.83% to 4.52%.

scholarly journals DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF HYDROQUINONE IN BULK, MARKETED CREAM AND PREAPARED NLC FORMULATION

2017 ◽  
Vol 9 (5) ◽  
pp. 102
Author(s):  
Sukhjinder Kaur ◽  
Taranjit Kaur ◽  
Gurdeep Kaur ◽  
Shivani Verma
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Pure Form ◽  
Nanostructured Lipid Carrier ◽  
Limit Of Quantification ◽  
Double Beam ◽  
Uv Visible ◽  
Development And Validation

Objective: The aim of the present work was to develop a simple, rapid, accurate and economical UV-visible spectrophotometric method for the determination of hydroquinone (HQ) in its pure form, marketed formulation as well as in the prepared nanostructured lipid carrier (NLC) systems and to validate the developed method.Methods: HQ was estimated at UV maxima of 289.6 nm in pH 5.5 phosphate buffer using UV-Visible double beam spectrophotometer. Following the guidelines of the International Conference on Harmonization (ICH), the method was validated for various analytical parameters like linearity, precision, and accuracy robustness, ruggedness, limit of detection, quantification limit, and formulation analysis.Results: The obtained results of the analysis were validated statistically. Recovery studies were performed to confirm the accuracy of the proposed method. In the developed method, linearity over the concentration range of 5-40 μg/ml of HQ was observed with the correlation coefficient of 0.998 and found in good agreement with Beer Lambert’s law. The precision (intra-day and inter-day) of the method was found within official RCD limits (RSD<2%).Conclusion: The sensitivity of the method was assessed by determining the limit of detection and limit of quantification. It could be concluded from the results obtained that the purposed method for estimation of HQ in pure form, in the marketed ointment and in the prepared NLC-formulation was simple, rapid, accurate, precise and economical. It can be used successfully in the quality control of pharmaceutical formulations and for the routine laboratory analysis.

Design of a novel optical sensor for determination of trace amounts of copper by UV–visible spectrophotometry in real samples

2017 ◽  
Vol 32 (3) ◽  
pp. e4110
Author(s):  
Eslam Pourbasheer ◽  
Somayeh Morsali ◽  
Zhila Azari ◽  
Mohammad Ali Karimi ◽  
Mohammad Reza Ganjali
Keyword(s):  
Optical Sensor ◽  
Real Samples ◽  
Visible Spectrophotometry ◽  
Uv Visible

scholarly journals STABILITY INDICATING HPLC METHOD FOR DETERMINATION OF VILAZODONE HYDROCHLORIDE

2017 ◽  
Vol 9 (4) ◽  
pp. 123
Author(s):  
Birva A. Athavia ◽  
Zarna R. Dedania ◽  
Ronak R. Dedania ◽  
S. M. Vijayendra Swamy ◽  
Chetana B. Prajapati
Keyword(s):  
Limit Of Detection ◽  
Degradation Products ◽  
Hplc Method ◽  
Alkaline Condition ◽  
Forced Degradation ◽  
Molecular Formula ◽  
Stability Indicating ◽  
Dry Heat ◽  
Limit Of Quantitation

Objective: The aim and objective of this study was to develop and validate Stability Indicating HPLC method for determination of Vilazodone Hydrochloride.Methods: The method was carried out on a Phenomenex, C18 (250x4.6 mm, 5 µm) Column using a mixture of Acetonitrile: Water (50:50v/v), pH adjusted to 3.3 with Glacial Acetic Acid for separation. The flow rate was adjusted at 1 ml/min and Detection was carried out at 240 nm.Results: The retention time of vilazodone hydrochloride was found to be 2.3 min. The calibration curve was found to be linear in the range 25-75µg/ml with a correlation coefficient (R2=0.996). The limit of detection and limit of quantitation were found to be 4.78µg/ml and 14.48µg/ml respectively. The % recovery of vilazodone hydrochloride was found to be in the range of 98.21±0.08 % to 99.07±0.64%. The proposed method was successfully applied for the estimation of vilazodone hydrochloride in marketed tablet formulation.Vilazodone Hydrochloride was subjected to forced degradation under Acidic, Alkaline, Oxidation, Dry Heat and Photolytic degradation conditions. Vilazodone hydrochloride showed 3.12% degradation under acidic condition, 4.78% under alkaline condition, 7.8% under oxidation condition, 3.53% under dry heat condition and 4.9% under photolytic condition.Acid degradation impurity was identified and characterised by LC-MS/MS was found to be 1-(4-Penten-1-yl) piperazine having molecular weight 154.253 (m/z 155.08) and Molecular Formula C9H18N2.Conclusion: A simple, precise, rapid and accurate Stability Indicating HPLC method has been developed and validated for the determination of Vilazodone Hydrochloride in presence of its degradation products as per the ICH Guidelines. 

scholarly journals SENSITIVE DETERMINATION OF RELATED SUBSTANCES IN PIOGLITAZONE HYDROCHLORIDE BY HPLC

2017 ◽  
Vol 9 (2) ◽  
pp. 34
Author(s):  
N. Balaji ◽  
Sayeeda Sultana
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Internal Diameter ◽  
Related Substances ◽  
Relative Standard ◽  
Limit Of Quantitation

Objective: An efficient, high performance liquid chromatographic method has been developed and validated for the quantification of related substances in pioglitazone hydrochloride drug substance.Methods: This method includes the determination of three related substances in pioglitazone hydrochloride. The mobile phase A is 0.1% w/v triethylamine in water with pH 2.5 adjusted by dilute phosphoric acid. The mobile phase B is premixed and degassed mixtures of acetonitrile and methanol. The flow rate was 1 ml/min. The elution used was gradient mode. The HPLC column used for the analysis was symmetry C18 with a length of 250 mm, the internal diameter of 4.6 mm and particle size of 5.0 microns.Results: The developed method was found to be linear with the range of 0.006-250% with a coefficient of correlation 0.99. The precision study revealed that the percentage relative standard deviation was within the acceptable limit. The limit of detection and limit of quantitation of the impurities was less than 0.002%and 0.006% with respect to pioglitazone hydrochloride test concentration of 2000 µg/ml respectively. This method has been validated as per ICH guidelines Q2 (R1).Conclusion: A reliable, economical HPLC method was magnificently established for quantitative analysis of related substances of pioglitazone hydrochloride drug substance.

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