Associations of Serum Skeletal Alkaline Phosphatase with Elevated C-Reactive Protein and Mortality

The objective of this study was to investigate the potential benefit of branched chain amino acid (BCAA)-enriched total parenteral nutrition (TPN) for malnourished surgical patients with gastrointestinal cancer. Sixty-four malnourished patients with gastrointestinal cancer with elective surgical intervention were equally allocated into two groups to receive isonitrogenous and iso-caloric TPN. All of them must have received TPN soon after surgery and were subsequently randomized and divided into either BCAA-enriched TPN (30% BCAA; intervention group) or standard TPN (24% BCAA; control group). Patient biochemical data, nutritional parameters, and clinical outcome were collected to analyze the significance of BCAA-enriched TPN. After a period of TPN, laboratory data, including white blood cell (WBC), C-reactive protein (CRP), alkaline phosphatase (ALK-P), and gamma-glutamyltransferase (γ-GT) levels, were significantly different between these two groups (all P < 0.05). Decreasing white blood cell and C-reactive protein levels were observed in the intervention group that might partially explain the reduced numbers of infectious complications. Furthermore, elevating alkaline phosphatase and gamma-glutamyltransferase levels being also less prominent might indicate a lesser hepatic burden by using BCAA-enriched TPN. The BCAA-enriched TPN can maintain a patient's serum visceral protein (albumin and prealbumin) and positive nitrogen balance better than standard TPN (all P < 0.05). Furthermore, a prominently decreased frequency of postoperative morbidity was also found in the BCAA-enriched TPN group (P = 0.021) despite no difference regarding surgical mortality. Our observations show that BCAA-enriched TPN may be beneficial in improving the nutritional status and reducing postoperative complications for malnourished patients with gastrointestinal cancer undergoing major surgery.

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Expression Of Nitric Oxide Synthase, Vascular Cell Adhesion Molecule and C- Reactive Protein Receptor In Sickle Cell Leucocytes: Relationship With Chronic Inflammation

Blood ◽

10.1182/blood.v122.21.2243.2243 ◽

2013 ◽

Vol 122 (21) ◽

pp. 2243-2243

Author(s):

Wendell Vilas Boas ◽

Bruno A. V. Cerqueira ◽

Thassila Nogueira Pitanga ◽

Sanzio Silva Santana ◽

Joao Oliveira Reis ◽

...

Keyword(s):

Nitric Oxide ◽

Alkaline Phosphatase ◽

Cell Adhesion Molecule ◽

Biochemical Profile ◽

Vascular Cell Adhesion ◽

C Reactive Protein ◽

Vascular Cell ◽

Reactive Protein ◽

Protein Receptor ◽

Oxide Synthase

Abstract Introduction Chronic and acute inflammatory phenomenon’s can contribute to activate several cells types and may play important role in the steady- and crisis-states of sickle cell anemia (HbSS) patients. Under normal circumstances, the nitric oxide synthase (NOS) constitutively produces low levels of nitric oxide (NO) that are important in the maintenance of vascular homeostasis, but in HbSS this balance is disrupted, mainly because the lack of arginine and the depletion of NO. This molecule also has an anti-inflammatory role in the vascular system and the state of resistance of NO can lead to deregulation of several other factors and interaction with many molecules. The aim of this study was correlate the expression of NOS, vascular cell adhesion molecule (VCAM), c-reactive protein receptor (rCRP) genes and biochemical profile of HbSS patients. Patients and Methods We studied 21 HbSS patients from Northeast Brazil, at steady state and were transfusion free. Blood samples were obtained during regular clinical visits. The expression of NOS, VCAM and rCRP genes were performed by real time PCR with Taqman assays from leucocytes RNA in trizol reagent. Serum biochemical analysis was determined using commercially available biochemical kits. The study was approved by the FIOCRUZ ethical committee and informed consents were signed by patients or official responsible. Results Our results showed a positive correlation among the expression of NOS and VCAM (p= 0.031, r=0.525), NOS and rCRP (p< 0.001, r=0.816) and VCAM and rCRP (p=0.003, r=0.659). Analyses of biochemical profile of HbSS individuals showed a correlation of NOS and serum calcium (p=0.044, r=0.456) as well as NOS and alkaline phosphatase (p=0.036, r=0.472). Discussion and Conclusion In this study, the correlation among genetic expression of NOS, VCAM and rCRP molecules suggests how factors involved to inflammation in HbSS are linked. In response to inflammation, the liver releases a variety of acute phase proteins. One such product, CRP, serves as a dominating diagnostic marker of inflammation. Chronic inflammatory reflects in the up-regulation of VCAM and VOC susceptibility, can increase the release of alkaline phosphatase, as well as deregulation of NOS expression due to impaired constitutive NOS activity with loss of endothelial NOS dimerization, increased NO scavenging by plasma hemoglobin and superoxide, increased arginase activity, and depleted intravascular nitrite reserves. Also, NOS couples electron flux for NO production by binding calmodulin, which is calcium-dependent, which explain the positive correlation between NOS and serum calcium. Furthermore, additional studies will elucidate the mechanism involved with the interaction of this complex network of molecule and the endothelial activation and dysfunction described in the pathophysiology of HbSS chronic and acute inflammatory. Disclosures: No relevant conflicts of interest to declare.

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Deficiency of 25-Hydroxyvitamin D and Dyslipidemia in Indian Subjects

Journal of Lipids ◽

10.1155/2013/623420 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 20

Author(s):

Jaydip Ray Chaudhuri ◽

K. Rukmini Mridula ◽

Alluri Anamika ◽

Demudu Babu Boddu ◽

Pradeep Kumar Misra ◽

...

Keyword(s):

Vitamin D ◽

Alkaline Phosphatase ◽

Vitamin D Deficiency ◽

Serum Glucose ◽

C Reactive Protein ◽

Hydroxyvitamin D ◽

Reactive Protein ◽

Calcium Phosphorus ◽

25 Hydroxyvitamin D ◽

Chemiluminescent Microparticle Immunoassay

Background. Vitamin D deficiency is widespread throughout the world. Several reports have incriminated vitamin D deficiency as the cause of rickets, osteomalacia, and other chronic diseases. Recent studies have suggested a possible link between deficiency of 25-hydroxyvitamin D and dyslipidemia.Aim. To investigate the association between 25-hydroxyvitamin D deficiency and dyslipidemia in Indian subjects.Methodology. We recruited 150 asymptomatic consecutive subjects from patients’ attendees at the Departments of Neurology and Medicine in Yashoda Hospital, Hyderabad, India. Study period was from October 2011 to March 2012. All subjects underwent 25-hydroxyvitamin D assay by chemiluminescent microparticle immunoassay, fasting blood sugar and lipid profile, calcium, phosphorus, alkaline phosphatase, and C-reactive protein (CRP).Results. Out of 150 subjects, men were 82 (54.6%), and mean age was 49.4 (±15.6) years. Among risk factors, hypertension was noted in 63/150 (42%), 25-hydroxyvitamin D deficiency in 59/150 (39.3%), diabetes in 45/150 (30%), dyslipidemia in 60 (40%), smoking in 35/150 (23.3%), and alcoholism in 27/150 (18%). Deficiency of 25-hydroxyvitamin D was significantly associated with dyslipidemia (P=0.0001), mean serum glucose (P=0.0002) mean CRP (P=0.04), and mean alkaline phosphatase (P=0.01). Multivariate analysis showed that 25-hydroxyvitamin D deficiency was independently associated with dyslipidemia (odds ratio: 1.9; 95% CI : 1.1–3.5).Conclusions. We found that deficiency of 25-hydroxyvitamin D was independently associated with dyslipidemia in Indian subjects.

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Alkaline phosphatase and percentage body fat predict circulating C-reactive protein in premenopausal women

Biomarkers ◽

10.3109/1354750x.2010.509811 ◽

2010 ◽

Vol 15 (8) ◽

pp. 663-670 ◽

Cited By ~ 10

Author(s):

Fatima Nayeem ◽

Karl E. Anderson ◽

Manubai Nagamani ◽

James J. Grady ◽

Lee-Jane W. Lu

Keyword(s):

Alkaline Phosphatase ◽

Body Fat ◽

Premenopausal Women ◽

C Reactive Protein ◽

Percentage Body Fat ◽

Reactive Protein

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Association between plasma alkaline phosphatase and C-reactive protein in Hong Kong Chinese

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2008.111 ◽

2008 ◽

Vol 46 (4) ◽

Cited By ~ 36

Author(s):

Bernard M.Y. Cheung ◽

Kwok Leung Ong ◽

Roberta V. Cheung ◽

Louisa Y.F. Wong ◽

Nelson M.S. Wat ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Hong Kong ◽

Hong Kong Chinese ◽

C Reactive Protein ◽

Reactive Protein ◽

Plasma Alkaline Phosphatase

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Dynamics of biochemical and immunological blood markers in patients with pseudoarthrosis of the femoral neck after total hip arthroplasty

Regulatory Mechanisms in Biosystems ◽

10.15421/021766 ◽

2017 ◽

Vol 8 (3) ◽

pp. 423-426 ◽

Cited By ~ 1

Author(s):

S. E. Bondarenko ◽

M. Akonjom ◽

V. A. Filipenko ◽

D. V. Morozenko ◽

A. Badnaoui ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Total Hip Arthroplasty ◽

Femoral Neck ◽

Hip Arthroplasty ◽

Sialic Acids ◽

Control Group ◽

C Reactive Protein ◽

Total Hip ◽

Reactive Protein ◽

Chondroitin Sulfates

The importance of biomarkers to predict recovery following total hip arthroplasty (THA) is still unclear to clinicians. To assess the preoperative biomarkers of patients with pseudoarthrosis of the femoral neck and their dynamics in the early postoperative period after THA, 50 patients aged 33 to 82 years old, 18 males and 32 females diagnosed with pseudoarthrosis of the femoral neck after failed internal fixation and failed conservative management were studied. The control group consisted of 30 healthy people aged 27 to 50 years, 13 males, 17 females. Patients’ blood was examined for biochemical markers upon admission, and then on the 7th and 14th days after surgery. Their blood serum content of total protein, albumin, glycoproteins, sialic acids, chondroitin sulfates, haptoglobin, glucose, cholesterol, triglycerides, ALT, AST, alkaline phosphatase, GGT, acid phosphatase, thymol index; interleukins (IL-1, IL-4 and IL-6.) and C-reactive protein was measured. The content of glycoproteins in the blood exceeded normal by 2.3 times, chondroitin sulfate by 4.7 times, sialic acids by 1.5 times, haptoglobin by 55.8%, fibrinogen by 19.1%, globulin by 19,6%, alkaline phosphatase activity by 72.3%, IL-1 by 94.7 and IL-6 by 3 times, C-reactive protein by 2.6 times. After THA there was a gradual decrease in blood biochemical and immunological markers. The most informative laboratory markers were glycoproteins, chondroitin sulfates, sialic acids, haptoglobin, activity of alkaline phosphatase, IL-1, IL-6 and IL-4, and C-reactive protein. Subsequent research is required to validate these dynamics.

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