PERFORMANCE OF CHROM AGAR MEDIUM AND CONVENTIONAL METHODS FOR DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS.

Background:- Methicillin resistant Staphylococcus aureus (MRSA) are responsible for hospital and community acquired infections. There are many laboratory methods for detection of MRSA. Chromogenic media have been used for the last few years for the quick detection of MRSA. Objective:- Aim of this study was to compare the performance of conventional methods and chromogenic media for the detection of MRSA in a tertiary care hospital. Material and method: - 200 consecutive isolates of S. aureus confirmed by conventional methods, collected in a tertiary care hospital were used for this study. Cefoxitin and oxacillin disc diffusion test used as conventional methods and Chromogenic media i.e. oxacillin resistant screen agar base (ORSAB) was used for detection of methicillin resistant Staphylococcus aureus. All confirmed MRSA were checked by gold standard mecA base PCR method. Result: - Out of 200 isolates of Staphylococcus aureus, 50,52 and 47 strains were MRSA by Cefoxitin disc diffusion method, oxacillin disc diffusion method and oxacillin resistant screen agar base (ORSAB) method respectively. Specificity was 100%, 98.66%, 98.66% by Cefoxitin disc diffusion, oxacillin disc diffusion and ORSAB method respectively. Conclusion: - In conclusion, cefoxitin disc diffusion was the best for the phenotypic detection of MRSA because their sensitivity and specificity were better than oxacillin and ORSAB.

Download Full-text

Detection of mrsa using three different methods such as oxacillin agar screening method, cefoxitin disc diffusion method and meca gene by pcr among clinical isolates of staphylococcus aureus from tertiary care hospital in kancheepuram dist

International Journal of Current Advanced Research ◽

10.24327/ijcar.2017.3458.0292 ◽

2017 ◽

Vol 6 (4) ◽

pp. 3455-3458

Author(s):

Mariyam Niyas F ◽

◽

Gopinath P ◽

Keyword(s):

Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Screening Method ◽

Meca Gene ◽

Clinical Isolates ◽

Diffusion Method ◽

Care Hospital ◽

Disc Diffusion ◽

Disc Diffusion Method

Download Full-text

Incidence of Vancomycin Resistant Phenotype of the Methicillin Resistant Staphylococcus aureus Isolated from a Tertiary Care Hospital in Lahore

Antibiotics ◽

10.3390/antibiotics9010003 ◽

2019 ◽

Vol 9 (1) ◽

pp. 3 ◽

Cited By ~ 4

Author(s):

Aqib Saeed ◽

Fatima Ahsan ◽

Muhammad Nawaz ◽

Khadeja Iqbal ◽

Kashif Rehman ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Resistance Gene ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Vancomycin Resistance ◽

Diffusion Method ◽

Care Hospital ◽

Methicillin Resistant ◽

Vancomycin Resistant

Staphylococcus aureus (S. aureus)-associated infections are one of the major threats to public health. The aim of the present study was to determine the antibiotic resistance pattern as well as the genetic characterization of methicillin and vancomycin resistant S. aureus (VRSA) isolated from a tertiary care hospital in Lahore. The S. aureus isolates were isolated from different clinical samples, identified by biochemical testing, and subjected to antibiotic susceptibility testing via the disc diffusion method or broth microdilution method. The methicillin resistance gene (mecA) and vancomycin resistance gene (vanA) were amplified by the polymerase chain reaction. The S. aureus isolates showed high incidences of resistance against methicillin (76%) and moderate incidences of resistance to vancomycin (14%). Isolates were also resistant to several other drugs, such as cefoxitin (76%), ertapenem (83%), ampicillin (81%), tobramycin (78%), moxifloxacin (76%), and tetracycline (74%). An encouraging finding was that 98% of isolates were susceptible to tigecycline, indicating its possible role in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) and VRSA, as well as the multi-drug resistant S. aureus. The mecA gene was detected in 33.3% of tested isolates (10/30), while the vanA gene was also detected in 30% (9/30) of the tested isolates. In conclusion, the frequent presence of methicillin and vancomycin resistance in S. aureus appraises the cautious use of these antibiotics in clinical practices. Furthermore, it is suggested that there should be continuous monitoring of tigecycline treatments in clinical setups in order to delay the development of resistance against it.

Download Full-text

METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) FROM CSOM IN A TERTIARY CARE HOSPITAL

10.36106/ijsr/9600960 ◽

2021 ◽

pp. 1-3

Author(s):

A.V. Kavitha ◽

Thyagarajan Ravinder ◽

Radhika Katragadda ◽

Leela Vajravelu

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Diffusion Method ◽

Care Hospital ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Gram Positive ◽

Suppurative Otitis Media ◽

Methicillin Resistant ◽

Sensitivity Pattern

Introduction: Chronic Suppurative Otitis Media (CSOM) is one of the major cause of preventable hearing loss if treated promptly. Emergence of resistant strains in the world is of great concern. The aim of the present study was to determine the aetiology and antibiotic sensitivity pattern of bacterial isolates from CSOM cases with special emphasis on Methicillin Resistant Staphylococcus aureus. Materials and methods: Ear swabs were collected from 212 patients attending otorhinolaryngology department, GKMC, Chennai was processed in Microbiology lab. Direct gram staining was done and then inoculated into Blood, MacConkey and Nutrient agar. Bacterial identification was done using standard microbiological techniques. Antibiotic susceptibility was done by Kirby Bauer disc diffusion technique. Methicillin Resistant Staphylococcus aureus (MRSA) was detected by Cefoxitin disc diffusion method as per CLSI guidelines. Results: 60 Gram positive bacteria were isolated from collected ear swabs. Staphylococcus aureus 46(26.59%) was the most common bacteria among Gram positive organism followed by CONS 9(5.20%) and Enterococci 5(2.89%). Staphylococcus aureus showed 41(89.13%) was sensitive to amikacin and 40(86.95%) to ofloxacin. All the Gram Positive cocci were 100% sensitive to Vancomycin and Linezolid. 19.53% were confirmed as MRSA by cefoxitin disc diffusion method. Conclusion: Microbial pattern can vary in different location in different periods of time. Hence knowledge of appropriate aetiology and antimicrobial resistance pattern of CSOM helps in rational use of antibiotics and control of drug resistance.MRSA is emerging as an important pathogen in CSOM. Empirical antibiotics should be directed to gram positives, and especially, MRSA should be taken into consideration.

Download Full-text

Prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Tertiary-Care Hospital in Eastern Nepal

Journal of Nepal Medical Association ◽

10.31729/jnma.309 ◽

2008 ◽

Vol 47 (170) ◽

Cited By ~ 12

Author(s):

Namrata Kumari ◽

TM Mohapatra ◽

YI Sigh

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Susceptibility ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Diffusion Method ◽

Care Hospital ◽

Susceptibility Pattern ◽

Methicillin Resistant ◽

Mrsa Strains

Nosocomial infection is a major problem in the world today. Methicillin-resistant Staphylococcus aureus (MRSA) strains, usually resistant to several antibiotics, shows a particular ability to spread inhospitals and is now present in most of the countries.The aim of the present study was to determine the prevalence of MRSA infections and theirantimicrobial susceptibility pattern in our hospital located in eastern Nepal.Identification of Staphylococcus aureus was confirmed by standard methods and the antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method. Interpretation criteriawere those of the national committee for clinical laboratory standards.During a period of one year, out of a total of 750 Staphylococcus aureus strains isolated from variousclinical samples, 196 (26.14%) were found to be Methicillin-resistant. Seventy percent isolates of MRSA were from inpatient departments and amongst them only 10% of the isolates were from intensive care units (ICU). More than 65% of MRSA were found to be resistant to Penicillin, Cephalosporins, Ciprofloxacin, Gentamicin Erythromycin and Tetracycline, while 47.96% of them were resistant to Amikacin. Many MRSA strains were multidrug-resistant. However, no strains were resistant toVancomycin.To reduce the prevalence of MRSA, the regular surveillance of hospital acquired infection, isolationnursing of patients who carry MRSA, monitoring of antimicrobial susceptibility pattern andformulation of a definite antibiotic policy may be helpful.Key words:eastern Nepal, resistant, tertiary-care hospital

Download Full-text

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS;

The Professional Medical Journal ◽

10.29309/tpmj/2019.26.04.3360 ◽

2019 ◽

Vol 26 (04) ◽

Author(s):

Aneela Khawaja ◽

Faiqa Arshad ◽

Rabiya Jamil

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistance ◽

Tertiary Care ◽

Blood Agar ◽

Diffusion Method ◽

Care Hospital ◽

Disc Diffusion ◽

Methicillin Resistant ◽

Sensitivity Pattern

Introduction: The versatility of Staphylococcus aureus has been transformed as “Methicillin resistant Staphylococcus aureus”. The most challenging are the disastrous virulence patterns being expressed due to the selection pressure of antibiotics. For assessing the prevalence of methicillin resistant Staphylococcus aureus; screening by cefoxitin disc (30µg) diffusion method is still a realistic approach among conventional phenotypic methods, being applied in most of the laboratories. This reliable and feasible technique contributes significantly for MRSA detection. This study has been designed to evaluate the prevalence and identify the sensitivity pattern of methicillin resistant Staphylococcus aureus isolates from different clinical specimens in a tertiary care hospital. Study Design: Descriptive study. Setting: Pathology Department, Microbiology Laboratory, PGMI. Period: January 2015 to December 2015. Materials & Methods: A total 713 clinical isolates of Staphylococcus aureus were processed. Identification and confirmation of Staphylococcus aureus was done by colony morphology on blood agar, gram stain, catalase, coagulase and DNA-ase tests. Screening for methicillin resistance was done using cefoxitin disc (30mg, OXOID); while different antibiotic discs were used to assess the sensitivity profile by Modified Kirby-Bauer Disc Diffusion method according to CLSI guidelines (2016). Results: Out of 713 Staphylococcus aureus isolates, 92 (12.90%) isolates were labelled as methicillin resistant by cefoxitin disc diffusion test. Out of 92 MRSA isolates, 57 (14.65%) were recovered from male patients and 35 (10.80%) from female patients. While, 60 (65.22%) MRSA isolates showed hemolysis on blood agar. Among 92 MRSA isolates, 41 (44.57%) were recovered from pus specimen. Resistance to trimethoprim/sulfamethoxazole was highest (65.22%) after penicillin (100%); while all the MRSA isolates were 100% sensitive to both vancomycin and linezolid. Conclusion: The prevalence of MRSA in hospital care settings is of great clinical concern. To combat this public health threat effectively, continuous surveillance of health-care associated infections, along with local antibiotic sensitivity pattern of MRSA; as well as formulation of a definite antibiotic policy is required.

Download Full-text