scholarly journals IN-VITRO CYTOTOXICITY ACTIVITY OF MALAXIS RHEEDEI SW METHANOL EXTRACT AGAINST HELA CELL LINE AND MCF- 7 CELL LINE.

2016 ◽  
Vol 9 (6) ◽  
pp. 244 ◽  
Author(s):  
Renjini Haridas
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Mtt Assay ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Plant Part ◽  
Hela Cell Line ◽  
Cytotoxicity Activity ◽  
Whole Plant ◽  
Ic50 Value

Cancer is a group of diseases caused by loss of cell cycle control. Cancer is associated with abnormal uncontrolled cell growth. The study was aimed to evaluation of the anticancer activity of the Malaxis rheedei Sw. on the HeLa cell line and MCF- 7cell line. The whole plant part of the Malaxis rheedei methanolic extract were tested for its inhibitory effect on HeLa Cell Line and MCF- 7cell line. The cytotoxicity of Malaxis rheedei on HeLa cell and MCF- 7cell line were evaluated by the MTT assay. Malaxis rheedei methanolic extract has significant cytotoxicity effect on MCF- 7cell line in concentration range between 18.75 to 300 µg/ml by using MTT assay and study also showed that inhibitory action on HeLa cell line inconcentration range between 18.75 to 300 µg/ml by using MTT assay. Methanol extract of  the whole plant part of Malaxis rheedei was found to be 7.3%, 16.6%,  25.4%, 36.3% and 47.1%toxic in HeLa cell line and 7.9%, 13.9%, 26%, 48.4% and 66.3% toxic in MCF- 7cell line. IC50 value of Malaxis rheedei on MCF- 7cell was  167.76  µg/ml and IC50 value of Malaxis rheedei on HeLa Cell was not found by MTT assay. From the performed assay, methanol extract of these drug shows greater activity on MCF- 7cell line and little activity on HeLa cell line and that mean Malaxis rheedei can be used as anticancer activity.Keywords: Cytotoxicity Activity, MTT Assay, Malaxis rheedei Sw. , HeLa CellLine, MCF- 7Cell Line.

scholarly journals Synergistic Cytotoxicity Effect by Combination of Methanol Extract of Parijoto Fruit (Medinilla speciosa Reinw. ex. Bl) and Cisplatin Against Hela Cell Line

2020 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Anif Nur Artanti ◽  
Umi Hanik Pujiastuti ◽  
Fea Prihapsara ◽  
Rita Rakhmawati
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
Hela Cell Line ◽  
Agent Based ◽  
Chemotherapy Agent ◽  
Ic50 Value ◽  
Synergistic Cytotoxicity ◽  
Combinational Treatment

As one of the leading causes of death in worldwide, cervical cancer requires the effective therapies to reduce its mortality rate. One of the chemotherapy agents that frequently used in the treatment is cisplatin. However, due to drug resistance and its side effects, an agent that can be combined with cisplatin is needed. Parijoto fruit (Medinilla speciosa Reinw.ex.Bl) contains secondary metabolites compounds that have potential as anticancer. The study aims to determine the cytotoxic effect of methanol extract of Parijoto fruit calculated from the IC50 value and the synergicity of the combinational treatment with cisplatin evaluated from the Combination Index (CI) value and its cell viability by using MTT assay. Results showed that methanol extract of Parijoto fruit (MEP) performed cytotoxic effect on HeLa cell line with IC50 of 209.6 μg/mL while the value of IC50 of cisplatin against HeLa cells amounted to 12.8 μg/mL. The combination of 26.205 ppm (1/8 IC50) of MEP and 1.601 ppm (1/8 IC50) of Cisplatin performed synergistic effect on HeLa cell line with the CI value of 0.69. From the above results, it can be concluded that MEP is potential as co-chemotherapy agent based on the synergistic cytotoxicity effect with cisplatin.Keyword: cytotoxic, Medinilla speciosa, cisplatin, co-chemotherapy, MTT

scholarly journals IN VITRO CYTOTOXICITY POTENTIAL OF ETHANOL EXTRACT OF SYZYGIUM SAMARANGENSE (Wt.)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-32
Author(s):  
Poonkodi K ◽  
Mini R ◽  
Vimaladevi K ◽  
Prabhu V ◽  
Anusuya M ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Hela Cell ◽  
Cell Line ◽  
Ethanol Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Line ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dependent Activity

The present investigation is carried out to study the invitro cytotoxicity of ethanol extract of Syzygium samarangense leaves on HeLa cell line by using MTT assay. Ethanol extract of S. samarangense showed concentration dependent activity on HeLa cell line with IC50 value of 40.5 μg/ml which shows that ethanol extract of S. samarangense posses significant cytoxicity.Moreover the preliminary phytochemical screening showed the presence of fatty acids, alkaloids, flavonoids, terphenoids, saponins, tannins and steroids which are responsible for its cytotoxicity. There are only a few reports are available for cytotoxicity of ethanol extract of S. samarangense.

scholarly journals Anticancer Evaluation of Plants from Indonesian Tropical Rain Forests

2011 ◽  
Vol 2 (3) ◽  
pp. 310
Author(s):  
Jamilah Abbas
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Line ◽  
Stem Bark ◽  
Tropical Rainforests ◽  
Hela Cell Line ◽  
Tropical Rain Forests ◽  
Rain Forests ◽  
Anticancer Activities ◽  
Ic50 Value

The anticancer activities of medicinal plants from Indonesia’s tropical rainforests were investigated against Hela cell line. Maytenfoliol from leaves of Calophyllumtetrapterum 3-epibetulinic acid from stem bark of C. tomentosum Wight and D.A-friedo-oleanan-3-on from stem bark of C. moonii showed anticancer activity. The compound 3-epibetulinic acid showed the more potent anticancer activity than maytenfoliol and D.A-friedo-oleanan-3- with thean IC50 value were 3.17 μg/mL, 4.89 μg/mL and 5.63 μg/mL, respectively.Keywords: Anticancer evaluation, Hela cell line, Calophyllumtetrapterum. C. tomentosum Wight and C. moonii

scholarly journals Invitro Cytotoxicity and Glucose Uptake Activity of Mushroom Pleurotus Eous in Methanol Extract Using L-6 Cell Line

2019 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Glucose Uptake ◽  
Cell Line ◽  
Cell Lines ◽  
Mtt Assay ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Colorimetric Assay ◽  
Antidiabetic Drug ◽  
Pleurotus Eous ◽  
Uptake Activity

The aim of the present study is to evaluate the cytotoxicity and glucose uptake activity of methanolic extract of pleurotus eous mushroom using L6 cell lines. An MTT assay is a colorimetric assay based on assessing the cell metabolic activityThe results showed that the extracts did not confer any cytotoxicity. The effects were observed in treated cells up to 6.25 mg/ml extract as measured with MTT. Also the mushroom extracts shows better glucose uptake potential. The results were compared with Insulin a standard antidiabetic drug. Insulin enhance the glucose uptake upto 97.8%. The L6 cell lines enhance the glucose uptake by 57.4% at 65µg concentration.

scholarly journals MTT Assay of Cissus Quadrangularison Hela Cell Line

2019 ◽  
Vol 8 (2S8) ◽  
pp. 1715-1718
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Mtt Assay ◽  
Hela Cell Line ◽  
Indigenous Plants

Approval of the helpful restorative properties of different indigenous plants has increased monstrous significance as they can be utilized as an elective wellspring of lead mixes in pharmacological ventures to handle numerous advanced issues, for example, tranquilize obstruction in microorganisms and nonparticularity in chemotherapeutic operators. Cissusquadrangularis Linn.is one such plant which has been generally utilized for therapeutic purposes in customary frameworks of prescription. Thus this work looks to approve the anticancer property of methanolic and ethanolic concentrates of the plant against HeLa cell line. The IC50 fixation was recorded at 62.5 ug/ml for methanolic separate and 125ug/ml for ethanolic remove. The outcomes unmistakably uncovered that the methanolic concentrate of Cissusquadrangularis was progressively powerful on HeLa cell line when contrasted with ethanolic extricate.

scholarly journals Cytotoxic Activity of Pentacyclic Triterpene-3-Heptadecanoate Ester against Hela Cell Line and Its Docking Study

2017 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Sri Mulyani Mulyadi ◽  
Tri Yuliati ◽  
Maulana Tegar ◽  
Imam Ardhila
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Docking Study ◽  
Heptadecanoic Acid ◽  
Hela Cell Line ◽  
Activity Assay ◽  
Pentacyclic Triterpene ◽  
Acetate Esters ◽  
Mtt Method ◽  
Ic50 Value

Pentacyclic triterpene-3-heptadecanoate (12,13-dihydro-α-amyrin-20,30-en-3-heptadecanoate)ester refers to the compound as a result of reaction between pentacyclic triterpene-3-ol(12,13-dihydro-α-amyrin-20,30-en-3-ol) and heptadecanoic acid. This research was aimed to conductcytotoxicity test of pentacyclic triterpene-3-heptadecanoate; 12,13-dihydro-α-amyrin-20,30-en-3-oland 12,13-dihydro-α-amyrin-20,30-en-3-acetate esters against Hela cell line. The activity assay wascarried out using MTT method. The results indicated that IC50 value of 12,13-dihydro-α-amyrin-3-heptadecanoate; 12,13-dihydro-α-amyrin-20,30-en-3-acetate and 12,13-dihydro-α-amyrin-20,30-en-3-ol were 111.0, 151.1 and 944.4 μg/ml, respectively. The compound of 12,13-dihydro-α-amyrin-20,30-en-3-heptadecanoate had the lowest IC50 value, suggesting it has a potency to be synthesized as ananticancer drug.

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